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The map label for this gene is mdeA [H]

Identifier: 86749784

GI number: 86749784

Start: 3049965

End: 3051245

Strand: Reverse

Name: mdeA [H]

Synonym: RPB_2667

Alternate gene names: 86749784

Gene position: 3051245-3049965 (Counterclockwise)

Preceding gene: 86749785

Following gene: 86749783

Centisome position: 57.23

GC content: 66.2

Gene sequence:

>1281_bases
ATGACCGAACGCAGCCCGGGATTTGCCACGCTCGCGGTTCACGCCGGCGCGCAGCCCGATCCCACCACCGGGGCGCGGGC
GACGCCGATCTACCAGACCACCTCCTTCGTGTTCAACGACGCCGACCACGCCGCCTCGCTGTTCGGCCTGCAGGCGTTCG
GCAACATCTACACCCGCATCACCAATCCGACGACGGCGGTGCTCGAGGAGCGCGTCGCCGCGCTCGAAGGCGGCACCGCG
GCGCTCGCGACCGCGTCCGGCCACGCCGCGCAGCTCGTGGTGATGCAGCAATTGCTGATGCCCGGCGACGAATTCATCGC
CGCGCGAAAACTCTACGGCGGCTCGATCAACCAGTTCACCCACGCCTTCAAGAGCTTCGGCTGGAACGTGGTGTGGGCCG
ATCCCGACGACATCGACAGCTTCCAGCGCGCGGTGACGCCGAAGACCAAGGCGATCTTCATCGAATCGATCGCCAATCCG
GCGGGCTCCATCACCGATATCGAGGCGATCGCCGAAGTCGCGCGCAGTGCCGGCGTGCCGCTGATCGTCGACAACACCCT
GGCGACGCCCTATCTGATCCGCCCGATCGACCACGGCGCCGACATCGTCGTGCATTCGCTGACGAAATTTCTCGGCGGCC
ACGGCAATTCGCTCGGCGGCATCATCGTCGACGCCGGCACCTTCGACTGGTCGAAGGGCGGCAAATATCCGATGCTGAGC
GAGCCGCGGCCGGAATATCACGGGCTGAAGCTGCAGGAGACGTTCGGCAATTTCGCCTTCGCGATCGCCTGCCGCGTGCT
CGGCCTGCGCGACCTCGGCCCGGCGCTGTCGCCGTTCAACGCCTTCCTGCTGATGACCGGCATCGAGACGCTGCCGCTGC
GGATGCAGAAGCATTGCGAGAACGCCAAGGCGATCGCCGAATTCCTGGCGACCCACAAGGCGGTGTCGGCGGTGAACTAT
TCCGGCCTGGCGTCGAGCAAGTACAATGCGCTGGCCCGCAAATACGCGCCGAAGGGCGCCGGCGCGGTGTTCACCTTCAG
CCTCAAGGGTGGCTACCAGGCCGGCGTCGATCTGGTCTCCAATGTGAAGCTGTTCTCGCATCTCGCCAATGTCGGCGACA
CCCGTTCGCTGATCATCCATCCGGCCTCGACCACCCACAGCCAGCTCGACGACGCGCAGAAGACGGCGGCCGGCGCCGCG
CCGGACATGGTGCGGGTGTCGATCGGCATCGAGGACAAGGAAGATCTGATCGCGGATCTCGACGGAGCGCTCGGCGGCTG
A

Upstream 100 bases:

>100_bases
CCTGACGCCGCCTCGCCCGTCGCTGCACCTCGCTTGACCTTCCGCCGGGTTGCGCTCAGATAGCGCCAAAGCATCAGCCA
GCTCGACGGAGGGATCGACC

Downstream 100 bases:

>100_bases
CGCCTGGCTGCGGCCGGGGCGGCCGGGAGGCGGCAATGAGTATCGGCCATCTCGAAATGCGCAGGATCGAGCAGCTCAGC
AACACCATTTTCGGTGTGGC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTERSPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRITNPTTAVLEERVAALEGGTA
ALATASGHAAQLVVMQQLLMPGDEFIAARKLYGGSINQFTHAFKSFGWNVVWADPDDIDSFQRAVTPKTKAIFIESIANP
AGSITDIEAIAEVARSAGVPLIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSLGGIIVDAGTFDWSKGGKYPMLS
EPRPEYHGLKLQETFGNFAFAIACRVLGLRDLGPALSPFNAFLLMTGIETLPLRMQKHCENAKAIAEFLATHKAVSAVNY
SGLASSKYNALARKYAPKGAGAVFTFSLKGGYQAGVDLVSNVKLFSHLANVGDTRSLIIHPASTTHSQLDDAQKTAAGAA
PDMVRVSIGIEDKEDLIADLDGALGG

Sequences:

>Translated_426_residues
MTERSPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRITNPTTAVLEERVAALEGGTA
ALATASGHAAQLVVMQQLLMPGDEFIAARKLYGGSINQFTHAFKSFGWNVVWADPDDIDSFQRAVTPKTKAIFIESIANP
AGSITDIEAIAEVARSAGVPLIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSLGGIIVDAGTFDWSKGGKYPMLS
EPRPEYHGLKLQETFGNFAFAIACRVLGLRDLGPALSPFNAFLLMTGIETLPLRMQKHCENAKAIAEFLATHKAVSAVNY
SGLASSKYNALARKYAPKGAGAVFTFSLKGGYQAGVDLVSNVKLFSHLANVGDTRSLIIHPASTTHSQLDDAQKTAAGAA
PDMVRVSIGIEDKEDLIADLDGALGG
>Mature_425_residues
TERSPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRITNPTTAVLEERVAALEGGTAA
LATASGHAAQLVVMQQLLMPGDEFIAARKLYGGSINQFTHAFKSFGWNVVWADPDDIDSFQRAVTPKTKAIFIESIANPA
GSITDIEAIAEVARSAGVPLIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSLGGIIVDAGTFDWSKGGKYPMLSE
PRPEYHGLKLQETFGNFAFAIACRVLGLRDLGPALSPFNAFLLMTGIETLPLRMQKHCENAKAIAEFLATHKAVSAVNYS
GLASSKYNALARKYAPKGAGAVFTFSLKGGYQAGVDLVSNVKLFSHLANVGDTRSLIIHPASTTHSQLDDAQKTAAGAAP
DMVRVSIGIEDKEDLIADLDGALGG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=410, Percent_Identity=31.7073170731707, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI299473759, Length=411, Percent_Identity=27.7372262773723, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI25453487, Length=410, Percent_Identity=27.8048780487805, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1790375, Length=413, Percent_Identity=30.7506053268765, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1789383, Length=424, Percent_Identity=25.9433962264151, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71980708, Length=418, Percent_Identity=31.5789473684211, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=410, Percent_Identity=31.219512195122, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=410, Percent_Identity=29.7560975609756, Blast_Score=181, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=45.3703703703704, Blast_Score=335, Evalue=7e-93,
Organism=Saccharomyces cerevisiae, GI6319307, Length=428, Percent_Identity=31.5420560747664, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=441, Percent_Identity=27.2108843537415, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=430, Percent_Identity=25.3488372093023, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321170, Length=362, Percent_Identity=25.6906077348066, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=30.7328605200946, Blast_Score=185, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 44997; Mature: 44866

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERSPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRI
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEHHHEECCCHHHHHHHHHHHHHHHHEEE
TNPTTAVLEERVAALEGGTAALATASGHAAQLVVMQQLLMPGDEFIAARKLYGGSINQFT
CCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHH
HAFKSFGWNVVWADPDDIDSFQRAVTPKTKAIFIESIANPAGSITDIEAIAEVARSAGVP
HHHHHCCCEEEECCCCCHHHHHHHCCCCHHEEEEEHHCCCCCCHHHHHHHHHHHHHCCCC
LIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSLGGIIVDAGTFDWSKGGKYPMLS
EEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCC
EPRPEYHGLKLQETFGNFAFAIACRVLGLRDLGPALSPFNAFLLMTGIETLPLRMQKHCE
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHC
NAKAIAEFLATHKAVSAVNYSGLASSKYNALARKYAPKGAGAVFTFSLKGGYQAGVDLVS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHH
NVKLFSHLANVGDTRSLIIHPASTTHSQLDDAQKTAAGAAPDMVRVSIGIEDKEDLIADL
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
DGALGG
HHCCCC
>Mature Secondary Structure 
TERSPGFATLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHAASLFGLQAFGNIYTRI
CCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEHHHEECCCHHHHHHHHHHHHHHHHEEE
TNPTTAVLEERVAALEGGTAALATASGHAAQLVVMQQLLMPGDEFIAARKLYGGSINQFT
CCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHH
HAFKSFGWNVVWADPDDIDSFQRAVTPKTKAIFIESIANPAGSITDIEAIAEVARSAGVP
HHHHHCCCEEEECCCCCHHHHHHHCCCCHHEEEEEHHCCCCCCHHHHHHHHHHHHHCCCC
LIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSLGGIIVDAGTFDWSKGGKYPMLS
EEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCC
EPRPEYHGLKLQETFGNFAFAIACRVLGLRDLGPALSPFNAFLLMTGIETLPLRMQKHCE
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHC
NAKAIAEFLATHKAVSAVNYSGLASSKYNALARKYAPKGAGAVFTFSLKGGYQAGVDLVS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHH
NVKLFSHLANVGDTRSLIIHPASTTHSQLDDAQKTAAGAAPDMVRVSIGIEDKEDLIADL
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
DGALGG
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]