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The map label for this gene is wcaJ [H]

Identifier: 86749776

GI number: 86749776

Start: 3040048

End: 3041577

Strand: Reverse

Name: wcaJ [H]

Synonym: RPB_2659

Alternate gene names: 86749776

Gene position: 3041577-3040048 (Counterclockwise)

Preceding gene: 86749777

Following gene: 86749773

Centisome position: 57.05

GC content: 63.86

Gene sequence:

>1530_bases
ATGATCGACGCCGCTGCGCAGGCCGCAGCGGGCAATGACACGATCCGGCCGACGGTGGAGCGGCGCAAGCGGCTGTCGCC
GGCGGCGCTCGCTGTGACCAACGAAAAAGTCCCCGAAGCCTATTCCCCGATCGTGATCGCCGGACTGGTGCGGCTCGCCG
ATTTCGTGCTGATCGCCGGCGTGGGCATCGCGCTGTATCTCGGCTACGTCGCGCGCCGCGACGGCGTGCATTGGGAGTAC
ATCGCCGCGATCATCGGAATGACCGTGACGGCCGTGATCAGCTTCCAGGCTGCCGACATCTACCAGGTCCAGGTGTTCCG
CGGCACGCTGAAGCAGATGACGCGGATGATCTCCGCGTGGTCGTTCGTGTTCTTGCTGTTCATCGGCGCATCCTTCTTCG
CCAAGCTCGGCGGCGACGTCTCGCGGCTGTGGCTGGCGTCGTTCTACGTCATCGGGCTCGCGCTATTGATCGTCGGTCGT
CTGGTTCTGCGCAATCTGGTCCGGCACTGGGCGCGCCAGGGCCGGCTCGACCGCCGCACCATCATCGTCGGCTCCGACGA
GAACGGCGAGCGATTGATCAACGCGCTGAAGGCGCAAGAGGACGACGATTCCGACATCCGCCTCCTCGGCGTGTTCGACG
ACCGCAACGATTCCCGCGCCCTCGACACCTGCGCGGGCAGCCCGAAGCTCGGCAAGATCGACGATATTCTCGAATTTGCC
CGACGCACCCGGGTCGATCTGGTGCTGTTCGCGCTTCCGATTTCGGCCGAGACCCGCATCCTCGACATGCTGAAGAAGCT
CTGGGTGCTACCGGTCGACATCCGGCTGTCGGCGCACACCAACAAGCTGCGCTTTCGCCCCCGCGCCTATTCGTATCTCG
GCAACGTGCCGACGCTCGAAGTGTTCGAGGCGCCGATCACCGATTGGGATCAGGTGACGAAGCGATTGTTCGATCACGTC
GTCGGCGGGCTGATCCTGCTCGCGGCCGCGCCGGTGATGGCGCTGGTTGCGCTGGCGATCAAGCTCGACAGCCCGGGCCC
GGTGCTGTTTCGTCAGAAACGGTTCGGTTTCAACAACGAGCGCATCGACGTCTTCAAGTTCCGCTCGATGTATCATCACC
TTGCCGACCCGACCGCGTCGAAGGTGGTGACCAGGAACGATCCGCGCGTCACCCGCGTCGGGAAATTCATCCGCCGCACC
AGCCTCGACGAATTGCCGCAGCTGTTCAACGTGGTGTTCAAGAGCAATCTGTCGCTGGTCGGCCCGCGGCCGCACGCCGT
TCAGGGCAAGCTGCAGCATCGGCTGTTCGACGAGACCGTCGACGGTTACTTCGCCCGCCACCGCGTCAAGCCGGGCATCA
CCGGCTGGGCCCAGATCAACGGTTGGCGCGGCGAGATCGACAACGAAGAGAAGATCCAGAAGCGCGTCGAGTTCGACCTG
TACTACATCGAAAACTGGTCGGTGCTGTTCGACCTGTTCATCCTCCTGAAGACGCCGTGGGCGCTGCTGAAGGGTGAGAA
CGCGTACTGA

Upstream 100 bases:

>100_bases
TTGCGATTTTTACCCTCTAGTCCGCCGAGGATTCGCGCGCGGAAGGGTCGCGCGCCGGCGACAACGGAATCGTGACGTGG
AACCGATCAGCGCCCGCACC

Downstream 100 bases:

>100_bases
CGCACGGAGAACTACGTCTTCCCGTACGACACCCCCATCCCGGCGCGGACGTCGGCCTGGATGCCATAAGGGGCGATCGG
GTAGCGCAGGCCGTTCTTGG

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MIDAAAQAAAGNDTIRPTVERRKRLSPAALAVTNEKVPEAYSPIVIAGLVRLADFVLIAGVGIALYLGYVARRDGVHWEY
IAAIIGMTVTAVISFQAADIYQVQVFRGTLKQMTRMISAWSFVFLLFIGASFFAKLGGDVSRLWLASFYVIGLALLIVGR
LVLRNLVRHWARQGRLDRRTIIVGSDENGERLINALKAQEDDDSDIRLLGVFDDRNDSRALDTCAGSPKLGKIDDILEFA
RRTRVDLVLFALPISAETRILDMLKKLWVLPVDIRLSAHTNKLRFRPRAYSYLGNVPTLEVFEAPITDWDQVTKRLFDHV
VGGLILLAAAPVMALVALAIKLDSPGPVLFRQKRFGFNNERIDVFKFRSMYHHLADPTASKVVTRNDPRVTRVGKFIRRT
SLDELPQLFNVVFKSNLSLVGPRPHAVQGKLQHRLFDETVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQKRVEFDL
YYIENWSVLFDLFILLKTPWALLKGENAY

Sequences:

>Translated_509_residues
MIDAAAQAAAGNDTIRPTVERRKRLSPAALAVTNEKVPEAYSPIVIAGLVRLADFVLIAGVGIALYLGYVARRDGVHWEY
IAAIIGMTVTAVISFQAADIYQVQVFRGTLKQMTRMISAWSFVFLLFIGASFFAKLGGDVSRLWLASFYVIGLALLIVGR
LVLRNLVRHWARQGRLDRRTIIVGSDENGERLINALKAQEDDDSDIRLLGVFDDRNDSRALDTCAGSPKLGKIDDILEFA
RRTRVDLVLFALPISAETRILDMLKKLWVLPVDIRLSAHTNKLRFRPRAYSYLGNVPTLEVFEAPITDWDQVTKRLFDHV
VGGLILLAAAPVMALVALAIKLDSPGPVLFRQKRFGFNNERIDVFKFRSMYHHLADPTASKVVTRNDPRVTRVGKFIRRT
SLDELPQLFNVVFKSNLSLVGPRPHAVQGKLQHRLFDETVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQKRVEFDL
YYIENWSVLFDLFILLKTPWALLKGENAY
>Mature_509_residues
MIDAAAQAAAGNDTIRPTVERRKRLSPAALAVTNEKVPEAYSPIVIAGLVRLADFVLIAGVGIALYLGYVARRDGVHWEY
IAAIIGMTVTAVISFQAADIYQVQVFRGTLKQMTRMISAWSFVFLLFIGASFFAKLGGDVSRLWLASFYVIGLALLIVGR
LVLRNLVRHWARQGRLDRRTIIVGSDENGERLINALKAQEDDDSDIRLLGVFDDRNDSRALDTCAGSPKLGKIDDILEFA
RRTRVDLVLFALPISAETRILDMLKKLWVLPVDIRLSAHTNKLRFRPRAYSYLGNVPTLEVFEAPITDWDQVTKRLFDHV
VGGLILLAAAPVMALVALAIKLDSPGPVLFRQKRFGFNNERIDVFKFRSMYHHLADPTASKVVTRNDPRVTRVGKFIRRT
SLDELPQLFNVVFKSNLSLVGPRPHAVQGKLQHRLFDETVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQKRVEFDL
YYIENWSVLFDLFILLKTPWALLKGENAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=372, Percent_Identity=37.6344086021505, Blast_Score=240, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 57456; Mature: 57456

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDAAAQAAAGNDTIRPTVERRKRLSPAALAVTNEKVPEAYSPIVIAGLVRLADFVLIAG
CCCCHHHHCCCCCCCCCHHHHHHCCCCHHEEEECCCCCCHHCHHHHHHHHHHHHHHHHHH
VGIALYLGYVARRDGVHWEYIAAIIGMTVTAVISFQAADIYQVQVFRGTLKQMTRMISAW
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SFVFLLFIGASFFAKLGGDVSRLWLASFYVIGLALLIVGRLVLRNLVRHWARQGRLDRRT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IIVGSDENGERLINALKAQEDDDSDIRLLGVFDDRNDSRALDTCAGSPKLGKIDDILEFA
EEEECCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHH
RRTRVDLVLFALPISAETRILDMLKKLWVLPVDIRLSAHTNKLRFRPRAYSYLGNVPTLE
HHCCCEEEEEEECCCCHHHHHHHHHHHHEECEEEEEECCCCCEEECCHHHHHHCCCCCHH
VFEAPITDWDQVTKRLFDHVVGGLILLAAAPVMALVALAIKLDSPGPVLFRQKRFGFNNE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCC
RIDVFKFRSMYHHLADPTASKVVTRNDPRVTRVGKFIRRTSLDELPQLFNVVFKSNLSLV
CCHHHHHHHHHHHHCCCCHHCCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEE
GPRPHAVQGKLQHRLFDETVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQKRVEFDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHEEE
YYIENWSVLFDLFILLKTPWALLKGENAY
EEECCHHHHHHHHHHHHCCHHHHCCCCCC
>Mature Secondary Structure
MIDAAAQAAAGNDTIRPTVERRKRLSPAALAVTNEKVPEAYSPIVIAGLVRLADFVLIAG
CCCCHHHHCCCCCCCCCHHHHHHCCCCHHEEEECCCCCCHHCHHHHHHHHHHHHHHHHHH
VGIALYLGYVARRDGVHWEYIAAIIGMTVTAVISFQAADIYQVQVFRGTLKQMTRMISAW
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SFVFLLFIGASFFAKLGGDVSRLWLASFYVIGLALLIVGRLVLRNLVRHWARQGRLDRRT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IIVGSDENGERLINALKAQEDDDSDIRLLGVFDDRNDSRALDTCAGSPKLGKIDDILEFA
EEEECCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHH
RRTRVDLVLFALPISAETRILDMLKKLWVLPVDIRLSAHTNKLRFRPRAYSYLGNVPTLE
HHCCCEEEEEEECCCCHHHHHHHHHHHHEECEEEEEECCCCCEEECCHHHHHHCCCCCHH
VFEAPITDWDQVTKRLFDHVVGGLILLAAAPVMALVALAIKLDSPGPVLFRQKRFGFNNE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCC
RIDVFKFRSMYHHLADPTASKVVTRNDPRVTRVGKFIRRTSLDELPQLFNVVFKSNLSLV
CCHHHHHHHHHHHHCCCCHHCCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEE
GPRPHAVQGKLQHRLFDETVDGYFARHRVKPGITGWAQINGWRGEIDNEEKIQKRVEFDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHEEE
YYIENWSVLFDLFILLKTPWALLKGENAY
EEECCHHHHHHHHHHHHCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]