Click here to switch to the map view.

The map label for this gene is rpoA [H]

Identifier: 86749439

GI number: 86749439

Start: 2667312

End: 2668331

Strand: Direct

Name: rpoA [H]

Synonym: RPB_2319

Alternate gene names: 86749439

Gene position: 2667312-2668331 (Clockwise)

Preceding gene: 86749438

Following gene: 86749440

Centisome position: 50.03

GC content: 63.82

Gene sequence:

>1020_bases
GTGACGATCCAGAAGAATTGGCAAGAACTCATTCGACCGAACAAGCTGCAGGTGACTGCCGGCAGCGATGCCACCCGGTT
CGCGACGCTGGTCGCCGAGCCGCTCGAGCGCGGCTTCGGCCAGACACTCGGCAACGCGCTGCGGCGCGTGCTGCTGTCGT
CGCTGCAGGGCGCGGCGGTGCAGTCGGTGCACATCGACGGCGTGCTGCACGAGTTCTCCTCGATCGCCGGCGTGCGCGAG
GACGTCACCGACATCGTGCTGAACATCAAGGACATCTCGCTGAAGATGCAGGGCGAAGGCCCGAAGCGGATGGTCGTCAA
GAAGCAGGGGCCGGGCGTCGTCACCGCCGGTGACATCCAGACCGTCGGCGACATCGTCGTGCTCAATCCGGACCTGCAGA
TCTGCACCCTCGACGAGGGCGCCGAGATCCGCATGGAATTCACCGTCAACACCGGCAAGGGCTACGTCGCCGCCGAGCGC
AACCGTCCGGAAGACGCGCCGATCGGCCTGATCCCGGTCGACAGCCTGTACTCTCCGGTGCGCAAGGTGTCGTACAAGGT
CGAGAACACCCGCGAGGGTCAGATCCTCGACTACGACAAGCTGACCATGACGGTCGAGACCAACGGCGCGCTGACCCCGG
ACGACGCGGTCGCCTTCGCCGCCCGCATCCTGCAGGACCAGCTCAACGTCTTCGTCAACTTCGAAGAGCCGCGCAAGGAA
GTCACCCAGGAGATCATCCCGGATCTGGCCTTCAACCCGGCCTTCCTCAAGAAGGTGGACGAACTCGAACTGTCGGTGCG
TTCGGCGAACTGCCTGAAGAACGACAACATCGTCTACATCGGCGACCTGGTGCAGAAGTCGGAAGCCGAAATGCTCCGCA
CCCCGAACTTCGGCCGCAAGTCGCTGAACGAGATCAAGGAAGTGCTGGCGCAGATGGGCCTGCATCTCGGCATGGAAGTG
CCGGGCTGGCCGCCGGAGAACATCGACGAGCTCGCCAAGCGCTTCGAAGATCACTACTGA

Upstream 100 bases:

>100_bases
CGCGGCTTTCGATACTTTGGCGCGGATTGATCCGCAGTTTCCATCGCCAGTGACCGCGCCCTTATTTCGGCGGCGATCGA
CCGGCCAGGAAGGTGACACA

Downstream 100 bases:

>100_bases
GAACGACGACGGGATGGCGGACAGCGCAGGCTGTTCGCCGTTCCCGTTTCCGGGCGAACGCAGGCAGCCCACCTGCAACT
CCGACGAACCGTCGGGACAT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTIQKNWQELIRPNKLQVTAGSDATRFATLVAEPLERGFGQTLGNALRRVLLSSLQGAAVQSVHIDGVLHEFSSIAGVRE
DVTDIVLNIKDISLKMQGEGPKRMVVKKQGPGVVTAGDIQTVGDIVVLNPDLQICTLDEGAEIRMEFTVNTGKGYVAAER
NRPEDAPIGLIPVDSLYSPVRKVSYKVENTREGQILDYDKLTMTVETNGALTPDDAVAFAARILQDQLNVFVNFEEPRKE
VTQEIIPDLAFNPAFLKKVDELELSVRSANCLKNDNIVYIGDLVQKSEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEV
PGWPPENIDELAKRFEDHY

Sequences:

>Translated_339_residues
MTIQKNWQELIRPNKLQVTAGSDATRFATLVAEPLERGFGQTLGNALRRVLLSSLQGAAVQSVHIDGVLHEFSSIAGVRE
DVTDIVLNIKDISLKMQGEGPKRMVVKKQGPGVVTAGDIQTVGDIVVLNPDLQICTLDEGAEIRMEFTVNTGKGYVAAER
NRPEDAPIGLIPVDSLYSPVRKVSYKVENTREGQILDYDKLTMTVETNGALTPDDAVAFAARILQDQLNVFVNFEEPRKE
VTQEIIPDLAFNPAFLKKVDELELSVRSANCLKNDNIVYIGDLVQKSEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEV
PGWPPENIDELAKRFEDHY
>Mature_338_residues
TIQKNWQELIRPNKLQVTAGSDATRFATLVAEPLERGFGQTLGNALRRVLLSSLQGAAVQSVHIDGVLHEFSSIAGVRED
VTDIVLNIKDISLKMQGEGPKRMVVKKQGPGVVTAGDIQTVGDIVVLNPDLQICTLDEGAEIRMEFTVNTGKGYVAAERN
RPEDAPIGLIPVDSLYSPVRKVSYKVENTREGQILDYDKLTMTVETNGALTPDDAVAFAARILQDQLNVFVNFEEPRKEV
TQEIIPDLAFNPAFLKKVDELELSVRSANCLKNDNIVYIGDLVQKSEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEVP
GWPPENIDELAKRFEDHY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=330, Percent_Identity=47.5757575757576, Blast_Score=296, Evalue=1e-81,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 37551; Mature: 37420

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQKNWQELIRPNKLQVTAGSDATRFATLVAEPLERGFGQTLGNALRRVLLSSLQGAAV
CCCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
QSVHIDGVLHEFSSIAGVREDVTDIVLNIKDISLKMQGEGPKRMVVKKQGPGVVTAGDIQ
EEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCEEEEEECCCCEEEECCCC
TVGDIVVLNPDLQICTLDEGAEIRMEFTVNTGKGYVAAERNRPEDAPIGLIPVDSLYSPV
CCCCEEEECCCEEEEEECCCCEEEEEEEEECCCEEEEECCCCCCCCCEEEEEHHHHHHHH
RKVSYKVENTREGQILDYDKLTMTVETNGALTPDDAVAFAARILQDQLNVFVNFEEPRKE
HHHHEEECCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCCHHHHH
VTQEIIPDLAFNPAFLKKVDELELSVRSANCLKNDNIVYIGDLVQKSEAEMLRTPNFGRK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHH
SLNEIKEVLAQMGLHLGMEVPGWPPENIDELAKRFEDHY
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TIQKNWQELIRPNKLQVTAGSDATRFATLVAEPLERGFGQTLGNALRRVLLSSLQGAAV
CCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
QSVHIDGVLHEFSSIAGVREDVTDIVLNIKDISLKMQGEGPKRMVVKKQGPGVVTAGDIQ
EEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCEEEEEECCCCEEEECCCC
TVGDIVVLNPDLQICTLDEGAEIRMEFTVNTGKGYVAAERNRPEDAPIGLIPVDSLYSPV
CCCCEEEECCCEEEEEECCCCEEEEEEEEECCCEEEEECCCCCCCCCEEEEEHHHHHHHH
RKVSYKVENTREGQILDYDKLTMTVETNGALTPDDAVAFAARILQDQLNVFVNFEEPRKE
HHHHEEECCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCCHHHHH
VTQEIIPDLAFNPAFLKKVDELELSVRSANCLKNDNIVYIGDLVQKSEAEMLRTPNFGRK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHH
SLNEIKEVLAQMGLHLGMEVPGWPPENIDELAKRFEDHY
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]