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The map label for this gene is livM [H]

Identifier: 86749392

GI number: 86749392

Start: 2622773

End: 2623771

Strand: Direct

Name: livM [H]

Synonym: RPB_2272

Alternate gene names: 86749392

Gene position: 2622773-2623771 (Clockwise)

Preceding gene: 86749391

Following gene: 86749393

Centisome position: 49.19

GC content: 68.07

Gene sequence:

>999_bases
ATGACGCCTGCCTCCGACACGGCCGGACCGGTCGCCACCGCCGGCCTGCGCAACGACCTGATCGCAGCCGCGGCGATCGC
CGGTCTCGGCGCGATCGGCTATTTCGTCTTCCCGGACGATCTCGCCTTCCTCACTCGCGTCATCGGTCTCGCATTTCTCG
TCATCTCGCTCGATCTCGTCACCGGCTATTGCGGCGTCGCGACGCTCGGCCACGCGGCGCTGTTCGGCGTCGGCGCCTAT
GCGGCCGGCAACGCCTGTCTGGCCGGCGTCACCGATCCGATCCTGATGCTGCTGGTCGGCATCGTCGCCGGCACCGCGAC
GGGGCTGATCTCCGGCGTGCTGGTCACGCGGTTTCGCGGCCTGCCGCAACTGGTGCTGTCGATCGCCTTCGGCCAATTGG
TCGCCGCGCTCGCCAACAAGCTGTCGTCGATCACCGGCGGCAGCGACGGTCTCGCCGGGATATCGCCGGGCCCGGTGTTC
GGCATGTTCAATTTCGACATGTTCGGCCGCACCGGCTACGTGTTCTCCGTCGCGGTGCTGCTGATCGCGATGGTGGTGCT
GCTGCGCTTCGCGCGCTCGCCGTTCGCGCTGATGTGCCGCGCGATCAAGGACGACACGTTGCGCGCGCGGATGATCGGCG
TCGCCGTCTATCCGCGGCTGGTGGTGATGTATTGCGTGGCCGGCGCCGTCGCCGGCCTGGGCGGCGCGCTGACGGCGATC
AACACCGGCGTGGTCGGCCTCGACAGCGTCGGCTTCGAGCGCTCGGCGGAAGCGCTGATCATGCTCATCCTGGGCGGCGC
CGGCAATCTGTGGGGCGCTCTGATCGGCGCCATCCTGTTCGAGCTGTTCCATCACTACGTATCCGCCGCCAGCCCGTTCC
ATTGGATGATCCTGATCGGCCTGCTGCTGATCGTGGTGGTGCTGTTCTTCCCCAAGGGCCTCGTCAACGAGGTCGTGCGG
CGGGTCGGCGAGTTTCGTCAGGCGAGGGGCGCGCGATGA

Upstream 100 bases:

>100_bases
GCAAGTATCTGGCCCCGGAATTCGGCGAGTTCTTCTTCTACGTTGCGGTGATCGTGATCGTGTACATCTTCCCCCACGGC
CTGTTCGGAAGGGCCCATTG

Downstream 100 bases:

>100_bases
CCGCCTTGCTCGACGTGCAGCAATTGTCCAAATCGTTCGGCGGCCTGCGGGTCACCGACGACGTGTCGTTCACGCTGTCG
AGCGGCGATCGCGTCGCGCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTPASDTAGPVATAGLRNDLIAAAAIAGLGAIGYFVFPDDLAFLTRVIGLAFLVISLDLVTGYCGVATLGHAALFGVGAY
AAGNACLAGVTDPILMLLVGIVAGTATGLISGVLVTRFRGLPQLVLSIAFGQLVAALANKLSSITGGSDGLAGISPGPVF
GMFNFDMFGRTGYVFSVAVLLIAMVVLLRFARSPFALMCRAIKDDTLRARMIGVAVYPRLVVMYCVAGAVAGLGGALTAI
NTGVVGLDSVGFERSAEALIMLILGGAGNLWGALIGAILFELFHHYVSAASPFHWMILIGLLLIVVVLFFPKGLVNEVVR
RVGEFRQARGAR

Sequences:

>Translated_332_residues
MTPASDTAGPVATAGLRNDLIAAAAIAGLGAIGYFVFPDDLAFLTRVIGLAFLVISLDLVTGYCGVATLGHAALFGVGAY
AAGNACLAGVTDPILMLLVGIVAGTATGLISGVLVTRFRGLPQLVLSIAFGQLVAALANKLSSITGGSDGLAGISPGPVF
GMFNFDMFGRTGYVFSVAVLLIAMVVLLRFARSPFALMCRAIKDDTLRARMIGVAVYPRLVVMYCVAGAVAGLGGALTAI
NTGVVGLDSVGFERSAEALIMLILGGAGNLWGALIGAILFELFHHYVSAASPFHWMILIGLLLIVVVLFFPKGLVNEVVR
RVGEFRQARGAR
>Mature_331_residues
TPASDTAGPVATAGLRNDLIAAAAIAGLGAIGYFVFPDDLAFLTRVIGLAFLVISLDLVTGYCGVATLGHAALFGVGAYA
AGNACLAGVTDPILMLLVGIVAGTATGLISGVLVTRFRGLPQLVLSIAFGQLVAALANKLSSITGGSDGLAGISPGPVFG
MFNFDMFGRTGYVFSVAVLLIAMVVLLRFARSPFALMCRAIKDDTLRARMIGVAVYPRLVVMYCVAGAVAGLGGALTAIN
TGVVGLDSVGFERSAEALIMLILGGAGNLWGALIGAILFELFHHYVSAASPFHWMILIGLLLIVVVLFFPKGLVNEVVRR
VGEFRQARGAR

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=301, Percent_Identity=26.9102990033223, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34233; Mature: 34102

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPASDTAGPVATAGLRNDLIAAAAIAGLGAIGYFVFPDDLAFLTRVIGLAFLVISLDLV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHH
TGYCGVATLGHAALFGVGAYAAGNACLAGVTDPILMLLVGIVAGTATGLISGVLVTRFRG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPQLVLSIAFGQLVAALANKLSSITGGSDGLAGISPGPVFGMFNFDMFGRTGYVFSVAVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHCCCHHHHHHHHH
LIAMVVLLRFARSPFALMCRAIKDDTLRARMIGVAVYPRLVVMYCVAGAVAGLGGALTAI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
NTGVVGLDSVGFERSAEALIMLILGGAGNLWGALIGAILFELFHHYVSAASPFHWMILIG
HCCCCCCCCCCCCHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLLIVVVLFFPKGLVNEVVRRVGEFRQARGAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TPASDTAGPVATAGLRNDLIAAAAIAGLGAIGYFVFPDDLAFLTRVIGLAFLVISLDLV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHH
TGYCGVATLGHAALFGVGAYAAGNACLAGVTDPILMLLVGIVAGTATGLISGVLVTRFRG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPQLVLSIAFGQLVAALANKLSSITGGSDGLAGISPGPVFGMFNFDMFGRTGYVFSVAVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHCCCHHHHHHHHH
LIAMVVLLRFARSPFALMCRAIKDDTLRARMIGVAVYPRLVVMYCVAGAVAGLGGALTAI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
NTGVVGLDSVGFERSAEALIMLILGGAGNLWGALIGAILFELFHHYVSAASPFHWMILIG
HCCCCCCCCCCCCHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLLIVVVLFFPKGLVNEVVRRVGEFRQARGAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]