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The map label for this gene is ytcI [H]

Identifier: 86749244

GI number: 86749244

Start: 2414132

End: 2415850

Strand: Direct

Name: ytcI [H]

Synonym: RPB_2123

Alternate gene names: 86749244

Gene position: 2414132-2415850 (Clockwise)

Preceding gene: 86749243

Following gene: 86749251

Centisome position: 45.28

GC content: 67.42

Gene sequence:

>1719_bases
ATGACGACATTCCAACAGGCCCGTTCCTTCCTGCTCGACCATCGCACCGACTACTCCGCCGCCGTCGCGGGTTTCCGCTG
GCCCGATCCGGTGCCGTTCAACTGGGCGCTCGACTGGTTCGATGCCGAGTTGGCGACGTCCGGCAGCCGTAACCGTCCCG
CGCTGTGGATCGTCGACGGCACCACCGGCCGGGAGGAGAAGCCGAGCTTCGCGCATCTGTCGAAGCGCTCGAACCAGGTC
GCGAACTATCTGCGTGCCAAGGGCCTGAAGCGCGGCGATCACCTGCTGCTGCTGCTCGGCAACGTCGTGCCGTTGTGGGA
GGTGATGCTGGCGGCGATCAAGCTCGGCCTCGTCACCATCCCGGCGACCACGCTGCTGACGCCGGACGAGTTGCGCGACC
GGCTCGAGCGCGGCCGCGCCAAGGCGGTGGTCGCTGCGCCCGACCAGATCGCCAAATTCAAGGGCCTCGGCGGCGACGAT
CTGCTGCGCGTCGTGGTCGGCGACGCCCAGGACGGCTGGCTCAATTACGACGGAACCGCGCTGCAGCCCGACGTCTTCAC
GCCGGACGGGCCGACGCAGGCTGACGATCCGATGCTGCTGTATTTCACCTCCGGCACCACGGCGAAGCCGAAGCTGGTGC
GGCACAGCCAGCGCAGCTATCCGGTCGGCGCGCTGTCGACGATGTTCTGGCTCGGGCTGCAGCCCGGCGACGTGCATCTC
AACATCTCGTCGCCGGGCTGGGCCAAGCACGCCTGGAGCTGCTTGTTCGCGCCGTGGAACGCCGGCGCCACCGTGTTCGT
CGTCAACCAGCCGCGGTTCGACGCCAAGAGTCTCTTGGCGACGATCGGCCGCTGCGGCGTCACCACGCTGTGCGCGCCGC
CGACGGTGTGGCGGATGTTCATTCAGGAGAAGCTCGCGGACTATCAAGTGTCGCTGCGCGAGGTCTGCGGCGCCGGCGAG
CCGCTCAATCCGGAAGTGATCGATCAGGTCAAGGCCGCCTGGGGCCTCACCATCCGCGATGGCTACGGCCAGACCGAGAC
CACCGCGATGGTTGGCAATTCGCCGGGGCAGGCAGTGAAGATCGGCTCGATGGGCCGGCCGATGCCGGGCTACGTCGTCA
AGATCACCGATGCGGACGGTCATCCGGCCAAGGAAGGCGAGATCACGCTGGCGCTGGGCGACGCGCGCCCGGCCGGCCTG
ATGCAGGGTTATCAAAGCGAGGGCGGCAAGCTCTCGGGCGCCGACGGCGACATCTATCGCTCCGGCGACGTCGCCTTCGC
CGACGACGACGGCTATCTCACCTTCGTCGGCCGCACCGACGACGTGTTCAAATCGTCGGACTATCGGATCTCGCCGTTCG
AACTGGAGAGCGTGCTGCTCGAACACGATGCCGTCGCCGAAGCCGCGGTGGTGCCGAGCCCGGACCCGATCAAGCTGGCG
ATCCCGAAGGCCTATGTGCTGCTGACGGCGGACGCGGAGCGCTCGCGCGACACCGCGCTGTCGATCTTCCGCCACATGCA
GGCGCGGATGGCGCCGTTCAAGCGCATCCGCAAGCTCGAACTGGTCACCGAACTGCCGAAGACGATCTCGGGCAAAATCC
GCCGCGTGCATCTGCGCCGCATCGAGCACGAGCACGACCAGACCGACTCGTTGCGCGGCGTCGAGTTTCGCGAAGAGGAT
TTCCCCGAGTTGAAAGCCGCGCCGACGCAGGAGGGGTGA

Upstream 100 bases:

>100_bases
ATCCTTCGAGACGCCCGGCCTCGCTCTGCGAGCGACGCCAGGCTCCTCAGGATGACGCCGGTTTGCGTAGCAGATGCTGT
CTCCTTGCGACGAGGGCGCC

Downstream 100 bases:

>100_bases
GGGCGGAGCGTACGTGCACCGTACTCTCGTTCGTCATTCCGGGATGCGCCCGCAGGGCGCAGGCCCGGAATCCATAACCC
CCATCCTATCGTTTTGATCA

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MTTFQQARSFLLDHRTDYSAAVAGFRWPDPVPFNWALDWFDAELATSGSRNRPALWIVDGTTGREEKPSFAHLSKRSNQV
ANYLRAKGLKRGDHLLLLLGNVVPLWEVMLAAIKLGLVTIPATTLLTPDELRDRLERGRAKAVVAAPDQIAKFKGLGGDD
LLRVVVGDAQDGWLNYDGTALQPDVFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHL
NISSPGWAKHAWSCLFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYQVSLREVCGAGE
PLNPEVIDQVKAAWGLTIRDGYGQTETTAMVGNSPGQAVKIGSMGRPMPGYVVKITDADGHPAKEGEITLALGDARPAGL
MQGYQSEGGKLSGADGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESVLLEHDAVAEAAVVPSPDPIKLA
IPKAYVLLTADAERSRDTALSIFRHMQARMAPFKRIRKLELVTELPKTISGKIRRVHLRRIEHEHDQTDSLRGVEFREED
FPELKAAPTQEG

Sequences:

>Translated_572_residues
MTTFQQARSFLLDHRTDYSAAVAGFRWPDPVPFNWALDWFDAELATSGSRNRPALWIVDGTTGREEKPSFAHLSKRSNQV
ANYLRAKGLKRGDHLLLLLGNVVPLWEVMLAAIKLGLVTIPATTLLTPDELRDRLERGRAKAVVAAPDQIAKFKGLGGDD
LLRVVVGDAQDGWLNYDGTALQPDVFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHL
NISSPGWAKHAWSCLFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYQVSLREVCGAGE
PLNPEVIDQVKAAWGLTIRDGYGQTETTAMVGNSPGQAVKIGSMGRPMPGYVVKITDADGHPAKEGEITLALGDARPAGL
MQGYQSEGGKLSGADGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESVLLEHDAVAEAAVVPSPDPIKLA
IPKAYVLLTADAERSRDTALSIFRHMQARMAPFKRIRKLELVTELPKTISGKIRRVHLRRIEHEHDQTDSLRGVEFREED
FPELKAAPTQEG
>Mature_571_residues
TTFQQARSFLLDHRTDYSAAVAGFRWPDPVPFNWALDWFDAELATSGSRNRPALWIVDGTTGREEKPSFAHLSKRSNQVA
NYLRAKGLKRGDHLLLLLGNVVPLWEVMLAAIKLGLVTIPATTLLTPDELRDRLERGRAKAVVAAPDQIAKFKGLGGDDL
LRVVVGDAQDGWLNYDGTALQPDVFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHLN
ISSPGWAKHAWSCLFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMFIQEKLADYQVSLREVCGAGEP
LNPEVIDQVKAAWGLTIRDGYGQTETTAMVGNSPGQAVKIGSMGRPMPGYVVKITDADGHPAKEGEITLALGDARPAGLM
QGYQSEGGKLSGADGDIYRSGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESVLLEHDAVAEAAVVPSPDPIKLAI
PKAYVLLTADAERSRDTALSIFRHMQARMAPFKRIRKLELVTELPKTISGKIRRVHLRRIEHEHDQTDSLRGVEFREEDF
PELKAAPTQEG

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=569, Percent_Identity=38.1370826010545, Blast_Score=361, Evalue=1e-99,
Organism=Homo sapiens, GI38505220, Length=529, Percent_Identity=39.6975425330813, Blast_Score=351, Evalue=1e-96,
Organism=Homo sapiens, GI122937307, Length=531, Percent_Identity=41.0546139359699, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI58082049, Length=551, Percent_Identity=35.5716878402904, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI157311624, Length=551, Percent_Identity=35.02722323049, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI157311622, Length=551, Percent_Identity=35.02722323049, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI115511026, Length=541, Percent_Identity=34.5656192236599, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI42544134, Length=399, Percent_Identity=34.8370927318296, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI195947378, Length=434, Percent_Identity=31.1059907834101, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI13375727, Length=522, Percent_Identity=26.6283524904215, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI8923896, Length=607, Percent_Identity=24.2174629324547, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI28416953, Length=521, Percent_Identity=25.1439539347409, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI253970489, Length=373, Percent_Identity=29.2225201072386, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI156151445, Length=535, Percent_Identity=26.9158878504673, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI187761345, Length=417, Percent_Identity=26.6187050359712, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI187761343, Length=417, Percent_Identity=26.6187050359712, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1790505, Length=566, Percent_Identity=27.5618374558304, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI221142682, Length=498, Percent_Identity=25.1004016064257, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786529, Length=540, Percent_Identity=26.2962962962963, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1786810, Length=543, Percent_Identity=26.5193370165746, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1788107, Length=564, Percent_Identity=23.758865248227, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI145693145, Length=499, Percent_Identity=24.8496993987976, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1786801, Length=433, Percent_Identity=24.7113163972286, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788595, Length=285, Percent_Identity=27.0175438596491, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=593, Percent_Identity=26.4755480607083, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71983001, Length=593, Percent_Identity=26.4755480607083, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=503, Percent_Identity=25.4473161033797, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=508, Percent_Identity=24.8031496062992, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71985884, Length=381, Percent_Identity=25.4593175853018, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=490, Percent_Identity=23.6734693877551, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=531, Percent_Identity=25.8003766478343, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=23.015873015873, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=496, Percent_Identity=22.5806451612903, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994694, Length=527, Percent_Identity=23.3396584440228, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71994703, Length=527, Percent_Identity=23.3396584440228, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71994690, Length=527, Percent_Identity=23.3396584440228, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560308, Length=383, Percent_Identity=28.4595300261097, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538037, Length=547, Percent_Identity=23.9488117001828, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=544, Percent_Identity=27.3897058823529, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6319264, Length=544, Percent_Identity=26.4705882352941, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319699, Length=351, Percent_Identity=22.7920227920228, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667955, Length=531, Percent_Identity=28.060263653484, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI62472339, Length=531, Percent_Identity=28.060263653484, Blast_Score=142, Evalue=7e-34,
Organism=Drosophila melanogaster, GI21355181, Length=509, Percent_Identity=27.3084479371316, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24667959, Length=491, Percent_Identity=28.7169042769857, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355225, Length=532, Percent_Identity=26.1278195488722, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18859661, Length=493, Percent_Identity=25.9634888438134, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922652, Length=500, Percent_Identity=24.6, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24583465, Length=532, Percent_Identity=23.8721804511278, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583463, Length=532, Percent_Identity=23.8721804511278, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364798, Length=532, Percent_Identity=23.8721804511278, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24581924, Length=516, Percent_Identity=24.2248062015504, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356947, Length=485, Percent_Identity=23.7113402061856, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648260, Length=360, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24656500, Length=496, Percent_Identity=24.7983870967742, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.1

Molecular weight: Translated: 62715; Mature: 62584

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFQQARSFLLDHRTDYSAAVAGFRWPDPVPFNWALDWFDAELATSGSRNRPALWIVDG
CCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEHHHCHHHHCCCCCCCCEEEEEEC
TTGREEKPSFAHLSKRSNQVANYLRAKGLKRGDHLLLLLGNVVPLWEVMLAAIKLGLVTI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHEEE
PATTLLTPDELRDRLERGRAKAVVAAPDQIAKFKGLGGDDLLRVVVGDAQDGWLNYDGTA
CHHHCCCHHHHHHHHHCCCCEEEEECCHHHHHHCCCCHHHEEEEEECCCCCCCCCCCCCE
LQPDVFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHL
ECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCEEE
NISSPGWAKHAWSCLFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMF
EECCCCCHHHHHEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHCCCEEECCCHHHHHHH
IQEKLADYQVSLREVCGAGEPLNPEVIDQVKAAWGLTIRDGYGQTETTAMVGNSPGQAVK
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCEEEECCCCCCCEEEEECCCCCCEEE
IGSMGRPMPGYVVKITDADGHPAKEGEITLALGDARPAGLMQGYQSEGGKLSGADGDIYR
ECCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEECCCCCCEEE
SGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESVLLEHDAVAEAAVVPSPDPIKLA
CCCEEEECCCCEEEEEECCCHHHCCCCCEECHHHHHHHHHHHHHHHHEEECCCCCCEEEE
IPKAYVLLTADAERSRDTALSIFRHMQARMAPFKRIRKLELVTELPKTISGKIRRVHLRR
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEHEHDQTDSLRGVEFREEDFPELKAAPTQEG
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTFQQARSFLLDHRTDYSAAVAGFRWPDPVPFNWALDWFDAELATSGSRNRPALWIVDG
CCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEHHHCHHHHCCCCCCCCEEEEEEC
TTGREEKPSFAHLSKRSNQVANYLRAKGLKRGDHLLLLLGNVVPLWEVMLAAIKLGLVTI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHEEE
PATTLLTPDELRDRLERGRAKAVVAAPDQIAKFKGLGGDDLLRVVVGDAQDGWLNYDGTA
CHHHCCCHHHHHHHHHCCCCEEEEECCHHHHHHCCCCHHHEEEEEECCCCCCCCCCCCCE
LQPDVFTPDGPTQADDPMLLYFTSGTTAKPKLVRHSQRSYPVGALSTMFWLGLQPGDVHL
ECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCEEE
NISSPGWAKHAWSCLFAPWNAGATVFVVNQPRFDAKSLLATIGRCGVTTLCAPPTVWRMF
EECCCCCHHHHHEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHCCCEEECCCHHHHHHH
IQEKLADYQVSLREVCGAGEPLNPEVIDQVKAAWGLTIRDGYGQTETTAMVGNSPGQAVK
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCEEEECCCCCCCEEEEECCCCCCEEE
IGSMGRPMPGYVVKITDADGHPAKEGEITLALGDARPAGLMQGYQSEGGKLSGADGDIYR
ECCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEECCCCCCEEE
SGDVAFADDDGYLTFVGRTDDVFKSSDYRISPFELESVLLEHDAVAEAAVVPSPDPIKLA
CCCEEEECCCCEEEEEECCCHHHCCCCCEECHHHHHHHHHHHHHHHHEEECCCCCCEEEE
IPKAYVLLTADAERSRDTALSIFRHMQARMAPFKRIRKLELVTELPKTISGKIRRVHLRR
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEHEHDQTDSLRGVEFREEDFPELKAAPTQEG
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; acetate; CoA

Specific reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

General reaction: Acid-thiol ligation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]