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The map label for this gene is livK [C]

Identifier: 86749202

GI number: 86749202

Start: 2364511

End: 2365695

Strand: Direct

Name: livK [C]

Synonym: RPB_2081

Alternate gene names: 86749202

Gene position: 2364511-2365695 (Clockwise)

Preceding gene: 86749200

Following gene: 86749203

Centisome position: 44.35

GC content: 65.91

Gene sequence:

>1185_bases
ATGAGTTTCGCTTCCCATATTTCGCGTCGCCTGTTGCTCGCGGCCGGCGCCGCGTCGCTGGCACTGATCGCTGTCGGGCC
GGCATCGGCCCAGGAGACCCTGAAGGTCGGCCTGGTGGCGGCGATGTCCGGCCAGTCGGCGAAGTCCGGCGAGGCCATCG
TCCGCGGTCTGTCGCTGGCGCTGGACGAGATCAACGCCAAGGGCGGCGTGCTCGGCAAGAAGCTGGAACTGGTGGTGCGC
GACGACGAGAGCAATCCCGCCAAGGGCGTGATCGCCGCGCGCGAGCTGGTGCAGCGCGAGAAGGTCGCCGCTTACTTCGG
CGGCATCGATACGCCGGTGTCGATGGCGATCGTGCCGTTCGCCAATCAGTCCAAGGTGCCGTTCATCGGCGTCTGGGCCG
CCGGTACCAAGATCACCCGCAACGGCGCGCCGGAGAACTACGTGTTCCGCGTCTCCGCGGTCGACGAACTGGTCGACATC
GCGCTGGTCGACTACGCGGTCAAGAAATACGGCGCCAAGAAGCCGGGCATGATCCTCATCAACAATCCCTGGGGCGAATC
CAACGAGGCCGGGCTGAAGAGCGCGCTCGACGCCAAGAAGATGACCGCCGCCGGCATCGAGAAATTCGAGACCGGCGACG
TCGACGTCGTGCCGCAGCTCACCCGGCTGAAGGACGCCGGCGCCGACACGCTGTTCATGGTCGCCAATGTCGCGCCCTCC
GCGCAGGTGGTGAAGTCGCTCGACCGGATGGGCTGGAGCGTGCCGGTGGTGTCGCATTGGGGCCCGGCCGGCGGGCGTTT
CACGGAGTTGGCCGGCCCCAGCGCCGAGAAGGTCCACTTCATCCAGACCTTCAGCTTCTCCGGCAACACCAGCCCGAAAG
CCGTGGCGCTGTTCGACGCGCTGAAGAAGAAATATCCCGAGGTCAAGACGGCCGCCGACGTCACCCCCGCGGTCGGCATC
GCCAATGCCTACGACGCCATGCATCTCACCGCGCTGGCGATCGCCAAGGCCGGCTCGACCGAAGGCCCGAAGGTCCGCGA
AGGCTTCTACCAGATCGGCAGCTATGACGGGCTGATCAAGACCTACAACAAGCCCTTCACCGCCGACAATCACGACGCGC
TGTCGCCCTCGGACTATCTGTTCACCTACTTCAAGGGCGCCGAGATCCTGCCGCTGACGAACTGA

Upstream 100 bases:

>100_bases
GTGGGCTTTCTCGCGAATTCCGGCGGCTTGGCGCCGATCTCCGGGATGGCACAGCGCTTGCTAGCCAGGATGCGATCTTT
CATCCTGCGAGGAGCCCGTG

Downstream 100 bases:

>100_bases
CGGCCCTCTTCCCTCTCCCCGCTCTTGGCGGGGAGAGGGTGGCTCGAAGCGGCGAAGCCGGTTCGAGTCGGGTGAGGGGC
CAGGCACGGCGCCGACCGAT

Product: twin-arginine translocation pathway signal

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSFASHISRRLLLAAGAASLALIAVGPASAQETLKVGLVAAMSGQSAKSGEAIVRGLSLALDEINAKGGVLGKKLELVVR
DDESNPAKGVIAARELVQREKVAAYFGGIDTPVSMAIVPFANQSKVPFIGVWAAGTKITRNGAPENYVFRVSAVDELVDI
ALVDYAVKKYGAKKPGMILINNPWGESNEAGLKSALDAKKMTAAGIEKFETGDVDVVPQLTRLKDAGADTLFMVANVAPS
AQVVKSLDRMGWSVPVVSHWGPAGGRFTELAGPSAEKVHFIQTFSFSGNTSPKAVALFDALKKKYPEVKTAADVTPAVGI
ANAYDAMHLTALAIAKAGSTEGPKVREGFYQIGSYDGLIKTYNKPFTADNHDALSPSDYLFTYFKGAEILPLTN

Sequences:

>Translated_394_residues
MSFASHISRRLLLAAGAASLALIAVGPASAQETLKVGLVAAMSGQSAKSGEAIVRGLSLALDEINAKGGVLGKKLELVVR
DDESNPAKGVIAARELVQREKVAAYFGGIDTPVSMAIVPFANQSKVPFIGVWAAGTKITRNGAPENYVFRVSAVDELVDI
ALVDYAVKKYGAKKPGMILINNPWGESNEAGLKSALDAKKMTAAGIEKFETGDVDVVPQLTRLKDAGADTLFMVANVAPS
AQVVKSLDRMGWSVPVVSHWGPAGGRFTELAGPSAEKVHFIQTFSFSGNTSPKAVALFDALKKKYPEVKTAADVTPAVGI
ANAYDAMHLTALAIAKAGSTEGPKVREGFYQIGSYDGLIKTYNKPFTADNHDALSPSDYLFTYFKGAEILPLTN
>Mature_393_residues
SFASHISRRLLLAAGAASLALIAVGPASAQETLKVGLVAAMSGQSAKSGEAIVRGLSLALDEINAKGGVLGKKLELVVRD
DESNPAKGVIAARELVQREKVAAYFGGIDTPVSMAIVPFANQSKVPFIGVWAAGTKITRNGAPENYVFRVSAVDELVDIA
LVDYAVKKYGAKKPGMILINNPWGESNEAGLKSALDAKKMTAAGIEKFETGDVDVVPQLTRLKDAGADTLFMVANVAPSA
QVVKSLDRMGWSVPVVSHWGPAGGRFTELAGPSAEKVHFIQTFSFSGNTSPKAVALFDALKKKYPEVKTAADVTPAVGIA
NAYDAMHLTALAIAKAGSTEGPKVREGFYQIGSYDGLIKTYNKPFTADNHDALSPSDYLFTYFKGAEILPLTN

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=326, Percent_Identity=22.6993865030675, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI48994941, Length=292, Percent_Identity=22.6027397260274, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41532; Mature: 41401

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFASHISRRLLLAAGAASLALIAVGPASAQETLKVGLVAAMSGQSAKSGEAIVRGLSLA
CCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHH
LDEINAKGGVLGKKLELVVRDDESNPAKGVIAARELVQREKVAAYFGGIDTPVSMAIVPF
HHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
ANQSKVPFIGVWAAGTKITRNGAPENYVFRVSAVDELVDIALVDYAVKKYGAKKPGMILI
CCCCCCCEEEEEECCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
NNPWGESNEAGLKSALDAKKMTAAGIEKFETGDVDVVPQLTRLKDAGADTLFMVANVAPS
ECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCH
AQVVKSLDRMGWSVPVVSHWGPAGGRFTELAGPSAEKVHFIQTFSFSGNTSPKAVALFDA
HHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHH
LKKKYPEVKTAADVTPAVGIANAYDAMHLTALAIAKAGSTEGPKVREGFYQIGSYDGLIK
HHHHCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHH
TYNKPFTADNHDALSPSDYLFTYFKGAEILPLTN
HCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCC
>Mature Secondary Structure 
SFASHISRRLLLAAGAASLALIAVGPASAQETLKVGLVAAMSGQSAKSGEAIVRGLSLA
CHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHH
LDEINAKGGVLGKKLELVVRDDESNPAKGVIAARELVQREKVAAYFGGIDTPVSMAIVPF
HHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
ANQSKVPFIGVWAAGTKITRNGAPENYVFRVSAVDELVDIALVDYAVKKYGAKKPGMILI
CCCCCCCEEEEEECCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
NNPWGESNEAGLKSALDAKKMTAAGIEKFETGDVDVVPQLTRLKDAGADTLFMVANVAPS
ECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCH
AQVVKSLDRMGWSVPVVSHWGPAGGRFTELAGPSAEKVHFIQTFSFSGNTSPKAVALFDA
HHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHH
LKKKYPEVKTAADVTPAVGIANAYDAMHLTALAIAKAGSTEGPKVREGFYQIGSYDGLIK
HHHHCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHH
TYNKPFTADNHDALSPSDYLFTYFKGAEILPLTN
HCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]