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The map label for this gene is glcD [H]

Identifier: 86749149

GI number: 86749149

Start: 2303502

End: 2304929

Strand: Direct

Name: glcD [H]

Synonym: RPB_2026

Alternate gene names: 86749149

Gene position: 2303502-2304929 (Clockwise)

Preceding gene: 86749147

Following gene: 86749150

Centisome position: 43.2

GC content: 67.23

Gene sequence:

>1428_bases
GTGGGCCTGACCATCACCAACACGCCGAAGCGCGCCGAGCCGCAGGCCGTGGCCAGCGCCATTGAAGCGCTCGCGGCGCG
GTTCGGCAACCGTCTCGTCACGTCGCTCGCGGTTCGCGAACAGCACGGCCACACCACCACCTGGCTGCCGAACCAGCCGC
CGGACGCGGTGGTGATGGCGCAGGAGACGGCGGACATCCAGGACGTGGTGCGCATTTGTGCCAAATACCGCGTGCCGGTG
ATCGCGTTCGGCACCGGCACCTCGCTGGAGGGCCACGTCAACGCCCCGGCTGGCGGCATTTCGATCGACCTGCGCGACAT
GAACAAGATCCTCAGCGTTCATGCCGAGGATCTCGACTGCGTGATCCAGCCCGGCGTCACCCGCAAGGCTCTGAACGAGG
ACCTTCGCGACCAGGGCCTGTTCTTCCCGATCGATCCGGGCGCCGACGCCTCGATCGGCGGCATGGCGGCGACGCGCGCC
TCCGGCACCAATGCGGTCCGCTACGGCACCATGCGCGACAACGTGCTGGCGCTGAAAGTCGTTCGCGGCGACGGCGAGAT
CATCACCACCGGCACCCGCGCCAAAAAGTCCGCCGCCGGCTACGACCTGACGCATCTGTTCGTCGGCAGCGAGGGCACGC
TCGGCATCATTTCGGAACTGACCATCAAGCTGCGCGGCATCCCCGAGGTGATCGCGGCGGCCTCGTGCTCGTTTTCGTCG
GTCACCGACGCCTGCCAGGCGGTGATCCTGGCGATCCAGACCGGCATCCCGCTGGCGCGGATCGAGCTGCTCAGCGAGTC
CCAGGTCAGGGCCGTCAACGCCTATTCCAAGCTGACGCTGCCGGAGACGCCGCTGCTGCTGCTGGAATTCCACGGCAGCG
AGGTCGAGGTCGGCGAGCAGTCGAAGAATTTCGGCGCGATCGCCAAGGATTGCGGCGGCGGCGACTTCACCTGGACGACG
CGGCCCGAAGACCGCAACAAGCTCTGGCAGGCCCGGCACGACGCCTATTGGTCGGTGCGGGCGCTGCGGCCCGGCGACGG
CGTCGGCGTGGTCGCCACCGATGTCTGCGTGCCGATCTCCCGGCTGGCCGATTGCGTCGCCGAGACTGAGCAGGACATGG
CGCGGCTCGGCCTGCTGGCGCCGATCGTCGGCCATGTCGGCGACGGCAATTTCCATTGCTCGCTGCTATGCGACGTCAAC
GACGCCGACGAGATGGCGCGCGCCGATGAGTTCATGCACCGTCTGGTCGAGCGGGCGCAGGCAATGGACGGCACCTGCAC
CGGCGAACACGGCATCGGTCAGGGCAAGCAGAAATATCTTCAAGCCGAACTCGGCATCGAGGCGCTGCAGGCGATGCGCG
CGATCAAGCAGGCGCTTGACCCGCAAAACATCTTCAATCCCGGCAAGATCCTGCCGCAAGGGCTTTGA

Upstream 100 bases:

>100_bases
CCGGCGTCGACGTTGCGATCTGCTTCCAAAGTCAAATGCTCCTCCCTTGACCCCTCCGGTATCCTTTGCCACGGTCGATC
CGATCCGGAGGATTTTTATC

Downstream 100 bases:

>100_bases
AGTGTTGATATTTCCGCCTCACCCTGATGTGCGGCGGAAATTGCTGTGAGTTCGCCATCATTCGTTGTTATGCGTTGATA
AGACGGAGCTAATGATCGTT

Product: D-lactate dehydrogenase (cytochrome)

Products: pyruvate; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MGLTITNTPKRAEPQAVASAIEALAARFGNRLVTSLAVREQHGHTTTWLPNQPPDAVVMAQETADIQDVVRICAKYRVPV
IAFGTGTSLEGHVNAPAGGISIDLRDMNKILSVHAEDLDCVIQPGVTRKALNEDLRDQGLFFPIDPGADASIGGMAATRA
SGTNAVRYGTMRDNVLALKVVRGDGEIITTGTRAKKSAAGYDLTHLFVGSEGTLGIISELTIKLRGIPEVIAAASCSFSS
VTDACQAVILAIQTGIPLARIELLSESQVRAVNAYSKLTLPETPLLLLEFHGSEVEVGEQSKNFGAIAKDCGGGDFTWTT
RPEDRNKLWQARHDAYWSVRALRPGDGVGVVATDVCVPISRLADCVAETEQDMARLGLLAPIVGHVGDGNFHCSLLCDVN
DADEMARADEFMHRLVERAQAMDGTCTGEHGIGQGKQKYLQAELGIEALQAMRAIKQALDPQNIFNPGKILPQGL

Sequences:

>Translated_475_residues
MGLTITNTPKRAEPQAVASAIEALAARFGNRLVTSLAVREQHGHTTTWLPNQPPDAVVMAQETADIQDVVRICAKYRVPV
IAFGTGTSLEGHVNAPAGGISIDLRDMNKILSVHAEDLDCVIQPGVTRKALNEDLRDQGLFFPIDPGADASIGGMAATRA
SGTNAVRYGTMRDNVLALKVVRGDGEIITTGTRAKKSAAGYDLTHLFVGSEGTLGIISELTIKLRGIPEVIAAASCSFSS
VTDACQAVILAIQTGIPLARIELLSESQVRAVNAYSKLTLPETPLLLLEFHGSEVEVGEQSKNFGAIAKDCGGGDFTWTT
RPEDRNKLWQARHDAYWSVRALRPGDGVGVVATDVCVPISRLADCVAETEQDMARLGLLAPIVGHVGDGNFHCSLLCDVN
DADEMARADEFMHRLVERAQAMDGTCTGEHGIGQGKQKYLQAELGIEALQAMRAIKQALDPQNIFNPGKILPQGL
>Mature_474_residues
GLTITNTPKRAEPQAVASAIEALAARFGNRLVTSLAVREQHGHTTTWLPNQPPDAVVMAQETADIQDVVRICAKYRVPVI
AFGTGTSLEGHVNAPAGGISIDLRDMNKILSVHAEDLDCVIQPGVTRKALNEDLRDQGLFFPIDPGADASIGGMAATRAS
GTNAVRYGTMRDNVLALKVVRGDGEIITTGTRAKKSAAGYDLTHLFVGSEGTLGIISELTIKLRGIPEVIAAASCSFSSV
TDACQAVILAIQTGIPLARIELLSESQVRAVNAYSKLTLPETPLLLLEFHGSEVEVGEQSKNFGAIAKDCGGGDFTWTTR
PEDRNKLWQARHDAYWSVRALRPGDGVGVVATDVCVPISRLADCVAETEQDMARLGLLAPIVGHVGDGNFHCSLLCDVND
ADEMARADEFMHRLVERAQAMDGTCTGEHGIGQGKQKYLQAELGIEALQAMRAIKQALDPQNIFNPGKILPQGL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=466, Percent_Identity=50, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI37595754, Length=489, Percent_Identity=47.6482617586912, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI119964728, Length=429, Percent_Identity=29.6037296037296, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI4501993, Length=229, Percent_Identity=33.1877729257642, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI1789351, Length=423, Percent_Identity=30.4964539007092, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787977, Length=498, Percent_Identity=25.7028112449799, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994907, Length=174, Percent_Identity=28.735632183908, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994914, Length=142, Percent_Identity=29.5774647887324, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71992373, Length=468, Percent_Identity=46.7948717948718, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71992381, Length=468, Percent_Identity=45.2991452991453, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71992392, Length=380, Percent_Identity=50.5263157894737, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17534361, Length=445, Percent_Identity=25.8426966292135, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17556096, Length=490, Percent_Identity=24.4897959183673, Blast_Score=108, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320027, Length=475, Percent_Identity=37.0526315789474, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6320023, Length=441, Percent_Identity=27.6643990929705, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320764, Length=476, Percent_Identity=24.5798319327731, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI18921117, Length=434, Percent_Identity=28.8018433179723, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24639277, Length=434, Percent_Identity=28.8018433179723, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24639275, Length=434, Percent_Identity=28.8018433179723, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24653753, Length=191, Percent_Identity=31.413612565445, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.2.4

Molecular weight: Translated: 50705; Mature: 50574

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTITNTPKRAEPQAVASAIEALAARFGNRLVTSLAVREQHGHTTTWLPNQPPDAVVMA
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEE
QETADIQDVVRICAKYRVPVIAFGTGTSLEGHVNAPAGGISIDLRDMNKILSVHAEDLDC
CCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEECCCCCCCEEEHHHHHHHHHHCCCCCCE
VIQPGVTRKALNEDLRDQGLFFPIDPGADASIGGMAATRASGTNAVRYGTMRDNVLALKV
EECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCCCCEEEECCCCCCEEEEEE
VRGDGEIITTGTRAKKSAAGYDLTHLFVGSEGTLGIISELTIKLRGIPEVIAAASCSFSS
EECCCCEEECCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHEEECCCHHHHHHHCCCCHH
VTDACQAVILAIQTGIPLARIELLSESQVRAVNAYSKLTLPETPLLLLEFHGSEVEVGEQ
HHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCC
SKNFGAIAKDCGGGDFTWTTRPEDRNKLWQARHDAYWSVRALRPGDGVGVVATDVCVPIS
CCCCCCHHHCCCCCCCEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEEHHHHHHHHH
RLADCVAETEQDMARLGLLAPIVGHVGDGNFHCSLLCDVNDADEMARADEFMHRLVERAQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
AMDGTCTGEHGIGQGKQKYLQAELGIEALQAMRAIKQALDPQNIFNPGKILPQGL
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GLTITNTPKRAEPQAVASAIEALAARFGNRLVTSLAVREQHGHTTTWLPNQPPDAVVMA
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEE
QETADIQDVVRICAKYRVPVIAFGTGTSLEGHVNAPAGGISIDLRDMNKILSVHAEDLDC
CCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEECCCCCCCEEEHHHHHHHHHHCCCCCCE
VIQPGVTRKALNEDLRDQGLFFPIDPGADASIGGMAATRASGTNAVRYGTMRDNVLALKV
EECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCCCCEEEECCCCCCEEEEEE
VRGDGEIITTGTRAKKSAAGYDLTHLFVGSEGTLGIISELTIKLRGIPEVIAAASCSFSS
EECCCCEEECCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHEEECCCHHHHHHHCCCCHH
VTDACQAVILAIQTGIPLARIELLSESQVRAVNAYSKLTLPETPLLLLEFHGSEVEVGEQ
HHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCC
SKNFGAIAKDCGGGDFTWTTRPEDRNKLWQARHDAYWSVRALRPGDGVGVVATDVCVPIS
CCCCCCHHHCCCCCCCEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEEHHHHHHHHH
RLADCVAETEQDMARLGLLAPIVGHVGDGNFHCSLLCDVNDADEMARADEFMHRLVERAQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
AMDGTCTGEHGIGQGKQKYLQAELGIEALQAMRAIKQALDPQNIFNPGKILPQGL
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; ferricytochrome c

Specific reaction: (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]