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The map label for this gene is mraW

Identifier: 86749109

GI number: 86749109

Start: 2255736

End: 2256749

Strand: Direct

Name: mraW

Synonym: RPB_1986

Alternate gene names: 86749109

Gene position: 2255736-2256749 (Clockwise)

Preceding gene: 86749107

Following gene: 86749110

Centisome position: 42.31

GC content: 71.3

Gene sequence:

>1014_bases
ATGAGCGAAGCGCGCCCGCGCCATATTCCCGTGCTGGGCCCCGAAGCGGTGACGCTGCTGGCGCCGCGCGATGGCGGAAT
CTATCTCGACGGCACCTTCGGCGCCGGCGGCTATAGCCGGCTGATCCTGGAGGCGGCCGCGACGCGGGTGATCGCGATCG
ATCGCGACCGCAGCGCCATCGCCGGCGGCTTCGACCTGGTCCAAGGCGCCGGCGGGCGGCTGACGCTGGTCGAGGATCGG
TTCTCCAATCTGGCCGAGGTCTGCGCGGCGCAGGGTGCGCCCGCCGTCGACGGCGTGGTGATGGATATCGGCGTGTCGTC
GATGCAGGTCGATCAGGCCGACCGCGGCTTCTCGTTCCGGCAGGACGGCCCGCTCGATATGCGGATGGGCACCGACGGGC
CGACTGCGGCCGACGTCGTCGCGCGCGCGTCCGAAACCGACCTCGCCAACATCATCTACATCTTCGGCGAGGAGAAATTC
TCGCGCCACGTCGCCCGCGCCGTCATCGCCGCCCGCAAGGAGGCGCCGATCACGACCACCAAGGCGCTGGCCGACATCGT
CGCCTCGGTGGTGCGCGCCAAGCCGGGACAGATCCACCCAGCGACGCGCACGTTCCAGGCGCTGCGGATCTTCGTCAACG
AAGAGCTCGACGAATTGTATCAGGCGCTGGCGGCGGCGGAGCGGGTGCTGAAACCGGGCGGGCGGCTCGCGGTGGTGACG
TTCCACTCGCTGGAAGACCGCATCGTCAAGACCTTCCTCGCCGAGCGCAGCCGCGTCGGCGGCGGCTCGCGGCATCTGCC
CGAATTGGCGCAGGCAGCTCCGAGCTTCACCGTGCTGACCAAGCGGCCGACGACCGCCGGCGACGCCGAGGTCGCCGCCA
ATCCGCGCGCGCGCTCGGCCAAGCTGCGCGGCGGCGAACGCACTGCGGCGCCGGCGCACCGGCCCGACGATCTGCCGGGA
TGGCCGACGCTCGCCGGTGTGATGAAGGCGGGCGTGACGAGGGCCGGCCGATGA

Upstream 100 bases:

>100_bases
CGATGCGCCCGTTGCCGCCGACATCGCAGCCGAACTGTGCCGAAGCCGGTCCCCGCACGCCGCGGACCGGTCGGACAGCC
GCGCCGTCAGGGCAGGGCGC

Downstream 100 bases:

>100_bases
TGCGCCTCATTCATGTCGTGGTGATCGGAATGCTGGTGTTCGCCGCGGCCTATGTCTACCGGATCAAGATGGAGTCGACC
GCGCGCACCGAGAAGGTGCT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSEARPRHIPVLGPEAVTLLAPRDGGIYLDGTFGAGGYSRLILEAAATRVIAIDRDRSAIAGGFDLVQGAGGRLTLVEDR
FSNLAEVCAAQGAPAVDGVVMDIGVSSMQVDQADRGFSFRQDGPLDMRMGTDGPTAADVVARASETDLANIIYIFGEEKF
SRHVARAVIAARKEAPITTTKALADIVASVVRAKPGQIHPATRTFQALRIFVNEELDELYQALAAAERVLKPGGRLAVVT
FHSLEDRIVKTFLAERSRVGGGSRHLPELAQAAPSFTVLTKRPTTAGDAEVAANPRARSAKLRGGERTAAPAHRPDDLPG
WPTLAGVMKAGVTRAGR

Sequences:

>Translated_337_residues
MSEARPRHIPVLGPEAVTLLAPRDGGIYLDGTFGAGGYSRLILEAAATRVIAIDRDRSAIAGGFDLVQGAGGRLTLVEDR
FSNLAEVCAAQGAPAVDGVVMDIGVSSMQVDQADRGFSFRQDGPLDMRMGTDGPTAADVVARASETDLANIIYIFGEEKF
SRHVARAVIAARKEAPITTTKALADIVASVVRAKPGQIHPATRTFQALRIFVNEELDELYQALAAAERVLKPGGRLAVVT
FHSLEDRIVKTFLAERSRVGGGSRHLPELAQAAPSFTVLTKRPTTAGDAEVAANPRARSAKLRGGERTAAPAHRPDDLPG
WPTLAGVMKAGVTRAGR
>Mature_336_residues
SEARPRHIPVLGPEAVTLLAPRDGGIYLDGTFGAGGYSRLILEAAATRVIAIDRDRSAIAGGFDLVQGAGGRLTLVEDRF
SNLAEVCAAQGAPAVDGVVMDIGVSSMQVDQADRGFSFRQDGPLDMRMGTDGPTAADVVARASETDLANIIYIFGEEKFS
RHVARAVIAARKEAPITTTKALADIVASVVRAKPGQIHPATRTFQALRIFVNEELDELYQALAAAERVLKPGGRLAVVTF
HSLEDRIVKTFLAERSRVGGGSRHLPELAQAAPSFTVLTKRPTTAGDAEVAANPRARSAKLRGGERTAAPAHRPDDLPGW
PTLAGVMKAGVTRAGR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=333, Percent_Identity=41.7417417417417, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=43.6170212765958, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=46.8152866242038, Blast_Score=246, Evalue=2e-66,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=40.1812688821752, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=40.1812688821752, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_RHOP2 (Q2IYL6)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485605.1
- ProteinModelPortal:   Q2IYL6
- STRING:   Q2IYL6
- GeneID:   3909492
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1986
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- ProtClustDB:   PRK00050
- BioCyc:   RPAL316058:RPB_1986-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35632; Mature: 35501

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 81-81 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEARPRHIPVLGPEAVTLLAPRDGGIYLDGTFGAGGYSRLILEAAATRVIAIDRDRSAI
CCCCCCCCCCEECCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCHH
AGGFDLVQGAGGRLTLVEDRFSNLAEVCAAQGAPAVDGVVMDIGVSSMQVDQADRGFSFR
HCCHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCCCCCC
QDGPLDMRMGTDGPTAADVVARASETDLANIIYIFGEEKFSRHVARAVIAARKEAPITTT
CCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHH
KALADIVASVVRAKPGQIHPATRTFQALRIFVNEELDELYQALAAAERVLKPGGRLAVVT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
FHSLEDRIVKTFLAERSRVGGGSRHLPELAQAAPSFTVLTKRPTTAGDAEVAANPRARSA
EHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCH
KLRGGERTAAPAHRPDDLPGWPTLAGVMKAGVTRAGR
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEARPRHIPVLGPEAVTLLAPRDGGIYLDGTFGAGGYSRLILEAAATRVIAIDRDRSAI
CCCCCCCCCEECCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCHH
AGGFDLVQGAGGRLTLVEDRFSNLAEVCAAQGAPAVDGVVMDIGVSSMQVDQADRGFSFR
HCCHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCCCCCC
QDGPLDMRMGTDGPTAADVVARASETDLANIIYIFGEEKFSRHVARAVIAARKEAPITTT
CCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHH
KALADIVASVVRAKPGQIHPATRTFQALRIFVNEELDELYQALAAAERVLKPGGRLAVVT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
FHSLEDRIVKTFLAERSRVGGGSRHLPELAQAAPSFTVLTKRPTTAGDAEVAANPRARSA
EHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCH
KLRGGERTAAPAHRPDDLPGWPTLAGVMKAGVTRAGR
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA