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The map label for this gene is 86749022

Identifier: 86749022

GI number: 86749022

Start: 2172720

End: 2174495

Strand: Direct

Name: 86749022

Synonym: RPB_1899

Alternate gene names: NA

Gene position: 2172720-2174495 (Clockwise)

Preceding gene: 86749021

Following gene: 86749023

Centisome position: 40.75

GC content: 63.23

Gene sequence:

>1776_bases
GTGTCGATCGCCGAGAGCCGGCCGAAGCGGCGTTTTCAACTGCTGCTGATCAAGCCGTCGCACTATGGCGAGGACGGCTA
CGTGATCCGCTGGTGGCGCGCCACGATCCCCTCGAATTCGCTGGCCAGCGTCTACGGCATCGCCCAGGAAAGCGCTGCGC
GCCAGGTGCTCGGCCCTGATGTCGAGATCGACATCGAGGCAATGGACGAGTTCAACACCAGGATCGATATTCCGAAGCTG
TTGAAACGGCTCGCCGCGCACGGCAATTTCGGCATGGTCGGCCTGATCGGCGTGCAGTCGAACCAGTATCCACGCGCGCT
CGACATCGCGAAGCCGTTCCGGGACGCCGGCATTCCGGTCGCGATCGGCGGTTTTCACGTCTCGGGTTGTTTGTCGATGC
TGGACGGCAAGGCGGTCGATCTCGACGCCTGCCGCGACCTCGGCGTGTCGATCTTCGCCGGGGAGGCGGAGGGCCGGATC
GATCTGCTGCTGCAGGATGCGGCGAAGGGCCGATTGCAGCCGCTGTACAACTACCTCGACGATCTGCCCGGCATCGAAGG
CACGCCGGTGCCGTTTCTGCCGATCGCCAACGTCAAGCGTACGCTCGGCCTGTCGACCTCGTTCGATGCCGGCCGCGGCT
GCCCGTATCAATGTTCGTTCTGTACGATCATCAATGTGCAGGGACGCAAGTCGCGCTACCGATCGGCCGACGATGTCGAG
GCGCTGGTGCGCGCCAACTGGCAGCAGGGCGTCTCCAAGTTCTTCATCACCGACGACAATTTCGCCCGCAACAAGGATTG
GGAGGCGATCCTCGACCGGCTGATCTGGCTGAAGGAAGAGCAAGGCATCCCGCTCGGCCTGATGATCCAGGTCGACACGC
TGTGCCACAAGATCCCGAACTTCGTCGAGAAATCCAAGCGCGCCGGCGTCACCCGCGTCTTTCTCGGCCTCGAAAGCATC
AACCCGGAGAATCTGCTGCAGGCCAAGAAGCGGCAGAACAAGATCACCGAATATCGCGCGATGCTGCTGGCCTGGCGGGC
CCAGGGCGTGATCACGCTGGCGGGCTACATCCTCGGCTTTCCGGCCGACACGCCGCAGAGCATCCTCGCCGATCTCGATA
TCATCAAGCGCGAATTGCCGATCGACATTCTGGAGTTCTTCTGCCTGACGCCGCTGCCGGGCTCGGAAGATCATCAGACG
CTGTGGAAGGCCGGGATCGCGATGGATGCCGACCTCAACAAATACGATATCGAACACGTCTGCACCGCCCATCCGAAGAT
GTCGCGGCAGGCGTGGGAAGATATCTATCAGCAGGCCTGGCGGCACTATTACGAACCTGCACATATCGAGACGCTGCTGC
GCCGCGCCGCAAAGAGCGGGATCAAGCTCGGCAGCATGGTCAAGCTGCTGCTGATCTTCTCGCTGGCGGTGTCGCTGGAG
AATGTTCATCCGTTGCAGAGCGGTATCTTCCGACTGCGGCATCCGTCGGAACGGCGGCCCGGCCTGCCGGCCGAACATGG
GCTATTGTTCTGGCCGCGGCTCGCGCTCGAACTGGCGCGCAAGAGCGGCGTGGTCCTGTCGACCGCGTTCGGGCTGAGCA
AGGTCGCCTACCGGGTGGCGCGCGACCCGATGCGTCATGACTACACCGATGCGGCGCTGACGCCGGTATCGGACGACGAC
GAGACCGAACTCGAGATGTTCACCCAAACCACCGCTGCAAAACAGGCGGTGGCGCACCGGAACAAGGTGCTGAGCCTGAC
CCGCGGCGCGGCGTAG

Upstream 100 bases:

>100_bases
GCTTGCGGCCGCGCGTCGTTCCCGGCAGCATCCGGCCGAATTCGTTTGTTCTCCGCCCGTCAGACTTCGTGTCACGACTG
CATTTGCAGGAGGATAGCTG

Downstream 100 bases:

>100_bases
CGCGGACCGTGGGCTGCGCCCGCAGCCAAGCGTCTGGCCCTAATTCACGATCGCGGTGCGACGGCGGCGAGCGGCGCAGC
GCATTCCCGGCCATGTCCAG

Product: radical SAM family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSIAESRPKRRFQLLLIKPSHYGEDGYVIRWWRATIPSNSLASVYGIAQESAARQVLGPDVEIDIEAMDEFNTRIDIPKL
LKRLAAHGNFGMVGLIGVQSNQYPRALDIAKPFRDAGIPVAIGGFHVSGCLSMLDGKAVDLDACRDLGVSIFAGEAEGRI
DLLLQDAAKGRLQPLYNYLDDLPGIEGTPVPFLPIANVKRTLGLSTSFDAGRGCPYQCSFCTIINVQGRKSRYRSADDVE
ALVRANWQQGVSKFFITDDNFARNKDWEAILDRLIWLKEEQGIPLGLMIQVDTLCHKIPNFVEKSKRAGVTRVFLGLESI
NPENLLQAKKRQNKITEYRAMLLAWRAQGVITLAGYILGFPADTPQSILADLDIIKRELPIDILEFFCLTPLPGSEDHQT
LWKAGIAMDADLNKYDIEHVCTAHPKMSRQAWEDIYQQAWRHYYEPAHIETLLRRAAKSGIKLGSMVKLLLIFSLAVSLE
NVHPLQSGIFRLRHPSERRPGLPAEHGLLFWPRLALELARKSGVVLSTAFGLSKVAYRVARDPMRHDYTDAALTPVSDDD
ETELEMFTQTTAAKQAVAHRNKVLSLTRGAA

Sequences:

>Translated_591_residues
MSIAESRPKRRFQLLLIKPSHYGEDGYVIRWWRATIPSNSLASVYGIAQESAARQVLGPDVEIDIEAMDEFNTRIDIPKL
LKRLAAHGNFGMVGLIGVQSNQYPRALDIAKPFRDAGIPVAIGGFHVSGCLSMLDGKAVDLDACRDLGVSIFAGEAEGRI
DLLLQDAAKGRLQPLYNYLDDLPGIEGTPVPFLPIANVKRTLGLSTSFDAGRGCPYQCSFCTIINVQGRKSRYRSADDVE
ALVRANWQQGVSKFFITDDNFARNKDWEAILDRLIWLKEEQGIPLGLMIQVDTLCHKIPNFVEKSKRAGVTRVFLGLESI
NPENLLQAKKRQNKITEYRAMLLAWRAQGVITLAGYILGFPADTPQSILADLDIIKRELPIDILEFFCLTPLPGSEDHQT
LWKAGIAMDADLNKYDIEHVCTAHPKMSRQAWEDIYQQAWRHYYEPAHIETLLRRAAKSGIKLGSMVKLLLIFSLAVSLE
NVHPLQSGIFRLRHPSERRPGLPAEHGLLFWPRLALELARKSGVVLSTAFGLSKVAYRVARDPMRHDYTDAALTPVSDDD
ETELEMFTQTTAAKQAVAHRNKVLSLTRGAA
>Mature_590_residues
SIAESRPKRRFQLLLIKPSHYGEDGYVIRWWRATIPSNSLASVYGIAQESAARQVLGPDVEIDIEAMDEFNTRIDIPKLL
KRLAAHGNFGMVGLIGVQSNQYPRALDIAKPFRDAGIPVAIGGFHVSGCLSMLDGKAVDLDACRDLGVSIFAGEAEGRID
LLLQDAAKGRLQPLYNYLDDLPGIEGTPVPFLPIANVKRTLGLSTSFDAGRGCPYQCSFCTIINVQGRKSRYRSADDVEA
LVRANWQQGVSKFFITDDNFARNKDWEAILDRLIWLKEEQGIPLGLMIQVDTLCHKIPNFVEKSKRAGVTRVFLGLESIN
PENLLQAKKRQNKITEYRAMLLAWRAQGVITLAGYILGFPADTPQSILADLDIIKRELPIDILEFFCLTPLPGSEDHQTL
WKAGIAMDADLNKYDIEHVCTAHPKMSRQAWEDIYQQAWRHYYEPAHIETLLRRAAKSGIKLGSMVKLLLIFSLAVSLEN
VHPLQSGIFRLRHPSERRPGLPAEHGLLFWPRLALELARKSGVVLSTAFGLSKVAYRVARDPMRHDYTDAALTPVSDDDE
TELEMFTQTTAAKQAVAHRNKVLSLTRGAA

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 65978; Mature: 65847

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAESRPKRRFQLLLIKPSHYGEDGYVIRWWRATIPSNSLASVYGIAQESAARQVLGPD
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
VEIDIEAMDEFNTRIDIPKLLKRLAAHGNFGMVGLIGVQSNQYPRALDIAKPFRDAGIPV
CEEEHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCE
AIGGFHVSGCLSMLDGKAVDLDACRDLGVSIFAGEAEGRIDLLLQDAAKGRLQPLYNYLD
EECCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCCEEEEEEHHHCCHHHHHHHHHH
DLPGIEGTPVPFLPIANVKRTLGLSTSFDAGRGCPYQCSFCTIINVQGRKSRYRSADDVE
HCCCCCCCCCCCEECCCHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCHHHCCCHHHHH
ALVRANWQQGVSKFFITDDNFARNKDWEAILDRLIWLKEEQGIPLGLMIQVDTLCHKIPN
HHHHCCHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHH
FVEKSKRAGVTRVFLGLESINPENLLQAKKRQNKITEYRAMLLAWRAQGVITLAGYILGF
HHHHHHHCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHCC
PADTPQSILADLDIIKRELPIDILEFFCLTPLPGSEDHQTLWKAGIAMDADLNKYDIEHV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHCCEECCCCCHHHHHHH
CTAHPKMSRQAWEDIYQQAWRHYYEPAHIETLLRRAAKSGIKLGSMVKLLLIFSLAVSLE
HCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NVHPLQSGIFRLRHPSERRPGLPAEHGLLFWPRLALELARKSGVVLSTAFGLSKVAYRVA
CCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
RDPMRHDYTDAALTPVSDDDETELEMFTQTTAAKQAVAHRNKVLSLTRGAA
HCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIAESRPKRRFQLLLIKPSHYGEDGYVIRWWRATIPSNSLASVYGIAQESAARQVLGPD
CCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
VEIDIEAMDEFNTRIDIPKLLKRLAAHGNFGMVGLIGVQSNQYPRALDIAKPFRDAGIPV
CEEEHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCE
AIGGFHVSGCLSMLDGKAVDLDACRDLGVSIFAGEAEGRIDLLLQDAAKGRLQPLYNYLD
EECCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCCEEEEEEHHHCCHHHHHHHHHH
DLPGIEGTPVPFLPIANVKRTLGLSTSFDAGRGCPYQCSFCTIINVQGRKSRYRSADDVE
HCCCCCCCCCCCEECCCHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCHHHCCCHHHHH
ALVRANWQQGVSKFFITDDNFARNKDWEAILDRLIWLKEEQGIPLGLMIQVDTLCHKIPN
HHHHCCHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHH
FVEKSKRAGVTRVFLGLESINPENLLQAKKRQNKITEYRAMLLAWRAQGVITLAGYILGF
HHHHHHHCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHCC
PADTPQSILADLDIIKRELPIDILEFFCLTPLPGSEDHQTLWKAGIAMDADLNKYDIEHV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHCCEECCCCCHHHHHHH
CTAHPKMSRQAWEDIYQQAWRHYYEPAHIETLLRRAAKSGIKLGSMVKLLLIFSLAVSLE
HCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NVHPLQSGIFRLRHPSERRPGLPAEHGLLFWPRLALELARKSGVVLSTAFGLSKVAYRVA
CCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
RDPMRHDYTDAALTPVSDDDETELEMFTQTTAAKQAVAHRNKVLSLTRGAA
HCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]