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The map label for this gene is glpK

Identifier: 86748886

GI number: 86748886

Start: 2016846

End: 2018351

Strand: Direct

Name: glpK

Synonym: RPB_1763

Alternate gene names: 86748886

Gene position: 2016846-2018351 (Clockwise)

Preceding gene: 86748874

Following gene: 86748889

Centisome position: 37.83

GC content: 68.73

Gene sequence:

>1506_bases
ATGCCTTTCGTGATGGCCATCGATCAGGGCACCACTTCGTCGCGCGCGATCCTGTTCCGTGGCGATATTTCGATCGCGGC
CTCGGCGCAGCAGGAATTCCCGCAGCATTTCCCCGCCTCCGGCTGGGTCGAGCACGAGCCCGAGGACATCTGGGCCTCGA
CGCTGGCGACCTGCCGGGCCGCGATGGAGAAGGCAGGGGCCACCGCCGCCGATATCGCCGCGATCGGCATCACCAACCAG
CGGGAGACCGTGGTGGTGTGGGACGCGGCGTCCGGCAAGGCGATCCACCGCGCCATCGTCTGGCAGGATCGCCGCACCGC
GGAGGTCTGCACCCGCATGAAGGCGGACGGCTATGAGCCGATGATCACCGACAAGACCGGGCTGATCATCGATCCGTATT
TCTCCGGCACCAAGGTCGCCTGGCTACTCGACAACGTGCCCGGCGCGCGCGCCCGCGCCGAGCGCGGCGAACTCAAATTC
GGCACCATCGATTGCTGGCTGCTGTGGCGGCTGACCGGCGGCCGCGTCCACGCCACCGACGCCACCAACGCCTCGCGCAC
GCTGCTGTTCAACATCCACACCGGCGATTGGGACGACGAGCTGCTGCAGCTGCTGCGCGTGCCGCGCTCGATGTTGCCGG
AGGTCAAGGATTCCTCCGCGCATTTCGGCGACAGCGCCTACGAACTGTTCGGTGCCCCGATCGCCATTCGCGGCATCGCC
GGCGACCAGCAGGCCGCCACCATCGGGCAGGCCTGTTTCTCGCCGGGCATGATCAAATCGACTTACGGCACCGGCTGCTT
CGCCCTGCTCATCACCGGCGCGACGCCGGTGAAGTCGCACAACAAGCTGCTCACCACCATCGCCTATCAGCTCGGCGGTA
AGCGTACCTACGCGCTGGAGGGCTCGATCTTCGTCGCGGGCTCGGCGGTGCAATGGCTGCGCGACGGCCTCGGCATCATC
AAGCACGCCAGCGAGACCGGCCCCCTCGCCGACAAATCCGATTCGATGCAGAGCGTTTATCTGGTGCCGGCCTTCGTCGG
CCTCGGCGCGCCGTACTGGAATCCGCGAGTGCGCGGCGCGCTGTTCGGCATGACGCGCAACACCGGCCCGGCCGAGCTGG
CGCACGCCGCGCTGGAGAGCGTCTGCTATCAGACCTACGATCTATGGGCGGCGATGCGCGCCGACTGGTCGGGCGCCGAC
GCCGCGCCGCCGGTGCTGCGCGTCGACGGCGGCATGGCGGCGTCGGACTGGACCATGCAGCGCCTCGCCGATCTCCTCGA
CGCCCCGGTAGACCGCCCCGTGATCCAGGAAACCACCGCGCTCGGCGCCGCGTATCTCGCCGGCCTCTCAGCCGGCGTCT
TCCCCGAGCCGCAGAAATTCGCCGACAACTGGCGCCTCGACCACCGCTTCAGGCCGGCGATGAGCCAGGCGACGCGCGAG
AGGAAGCTGGCGGGATGGGGCAGGGCGGTGAAGGGGCTGCTGGCGAGTGATGAGGGGGAGGGGTAG

Upstream 100 bases:

>100_bases
GGGCGGCGCGCAAGCCGGGCTGCCGATATTGACGCCGCAGGCGTGATCGGGTGGTGTGCGGCGAAACCCTTTCACACTCT
GCAAGCCGGGATGAACGCTC

Downstream 100 bases:

>100_bases
GCAACGATCGCTCTCTCTCTGGAGAGAGACCGCCTTATGAGTCGCGCCCCGATACCCTCCCGTCGTTCGTCATTCCGGGG
CGCGCGAAAGCGCGAACCCG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MPFVMAIDQGTTSSRAILFRGDISIAASAQQEFPQHFPASGWVEHEPEDIWASTLATCRAAMEKAGATAADIAAIGITNQ
RETVVVWDAASGKAIHRAIVWQDRRTAEVCTRMKADGYEPMITDKTGLIIDPYFSGTKVAWLLDNVPGARARAERGELKF
GTIDCWLLWRLTGGRVHATDATNASRTLLFNIHTGDWDDELLQLLRVPRSMLPEVKDSSAHFGDSAYELFGAPIAIRGIA
GDQQAATIGQACFSPGMIKSTYGTGCFALLITGATPVKSHNKLLTTIAYQLGGKRTYALEGSIFVAGSAVQWLRDGLGII
KHASETGPLADKSDSMQSVYLVPAFVGLGAPYWNPRVRGALFGMTRNTGPAELAHAALESVCYQTYDLWAAMRADWSGAD
AAPPVLRVDGGMAASDWTMQRLADLLDAPVDRPVIQETTALGAAYLAGLSAGVFPEPQKFADNWRLDHRFRPAMSQATRE
RKLAGWGRAVKGLLASDEGEG

Sequences:

>Translated_501_residues
MPFVMAIDQGTTSSRAILFRGDISIAASAQQEFPQHFPASGWVEHEPEDIWASTLATCRAAMEKAGATAADIAAIGITNQ
RETVVVWDAASGKAIHRAIVWQDRRTAEVCTRMKADGYEPMITDKTGLIIDPYFSGTKVAWLLDNVPGARARAERGELKF
GTIDCWLLWRLTGGRVHATDATNASRTLLFNIHTGDWDDELLQLLRVPRSMLPEVKDSSAHFGDSAYELFGAPIAIRGIA
GDQQAATIGQACFSPGMIKSTYGTGCFALLITGATPVKSHNKLLTTIAYQLGGKRTYALEGSIFVAGSAVQWLRDGLGII
KHASETGPLADKSDSMQSVYLVPAFVGLGAPYWNPRVRGALFGMTRNTGPAELAHAALESVCYQTYDLWAAMRADWSGAD
AAPPVLRVDGGMAASDWTMQRLADLLDAPVDRPVIQETTALGAAYLAGLSAGVFPEPQKFADNWRLDHRFRPAMSQATRE
RKLAGWGRAVKGLLASDEGEG
>Mature_500_residues
PFVMAIDQGTTSSRAILFRGDISIAASAQQEFPQHFPASGWVEHEPEDIWASTLATCRAAMEKAGATAADIAAIGITNQR
ETVVVWDAASGKAIHRAIVWQDRRTAEVCTRMKADGYEPMITDKTGLIIDPYFSGTKVAWLLDNVPGARARAERGELKFG
TIDCWLLWRLTGGRVHATDATNASRTLLFNIHTGDWDDELLQLLRVPRSMLPEVKDSSAHFGDSAYELFGAPIAIRGIAG
DQQAATIGQACFSPGMIKSTYGTGCFALLITGATPVKSHNKLLTTIAYQLGGKRTYALEGSIFVAGSAVQWLRDGLGIIK
HASETGPLADKSDSMQSVYLVPAFVGLGAPYWNPRVRGALFGMTRNTGPAELAHAALESVCYQTYDLWAAMRADWSGADA
APPVLRVDGGMAASDWTMQRLADLLDAPVDRPVIQETTALGAAYLAGLSAGVFPEPQKFADNWRLDHRFRPAMSQATRER
KLAGWGRAVKGLLASDEGEG

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI4504007, Length=506, Percent_Identity=43.6758893280632, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI189409120, Length=506, Percent_Identity=43.6758893280632, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI42794763, Length=512, Percent_Identity=43.1640625, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI41393575, Length=520, Percent_Identity=42.8846153846154, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI88196792, Length=507, Percent_Identity=30.3747534516765, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=51.7102615694165, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1789987, Length=490, Percent_Identity=25.1020408163265, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790005, Length=490, Percent_Identity=23.469387755102, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=38.0952380952381, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI71986140, Length=525, Percent_Identity=23.6190476190476, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71986147, Length=301, Percent_Identity=25.2491694352159, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321755, Length=484, Percent_Identity=34.9173553719008, Blast_Score=265, Evalue=2e-71,
Organism=Drosophila melanogaster, GI17864214, Length=464, Percent_Identity=43.1034482758621, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24655555, Length=503, Percent_Identity=37.9721669980119, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24655560, Length=503, Percent_Identity=37.9721669980119, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24655570, Length=503, Percent_Identity=37.9721669980119, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24655574, Length=503, Percent_Identity=37.9721669980119, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24655565, Length=503, Percent_Identity=37.9721669980119, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24654573, Length=397, Percent_Identity=43.8287153652393, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI19922058, Length=530, Percent_Identity=31.1320754716981, Blast_Score=251, Evalue=8e-67,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=32.1792260692464, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24656367, Length=510, Percent_Identity=27.0588235294118, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21357867, Length=522, Percent_Identity=25.6704980842912, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24656372, Length=346, Percent_Identity=27.1676300578035, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI45552909, Length=243, Percent_Identity=27.9835390946502, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_RHOP2 (Q2IZ89)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485382.1
- ProteinModelPortal:   Q2IZ89
- SMR:   Q2IZ89
- STRING:   Q2IZ89
- GeneID:   3909750
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1763
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   GWVEHEP
- ProtClustDB:   CLSK818559
- BioCyc:   RPAL316058:RPB_1763-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54058; Mature: 53927

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 11-11 BINDING 15-15 BINDING 81-81 BINDING 133-133 BINDING 242-242 BINDING 264-264 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFVMAIDQGTTSSRAILFRGDISIAASAQQEFPQHFPASGWVEHEPEDIWASTLATCRA
CCEEEEECCCCCCCCEEEEECCCEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AMEKAGATAADIAAIGITNQRETVVVWDAASGKAIHRAIVWQDRRTAEVCTRMKADGYEP
HHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCHHHHEEECCCCHHHHHHHHHCCCCCC
MITDKTGLIIDPYFSGTKVAWLLDNVPGARARAERGELKFGTIDCWLLWRLTGGRVHATD
EEECCCCCEEECCCCCCEEEEEEECCCCCHHHCCCCCEEECEEEEEEEEEECCCEEEECC
ATNASRTLLFNIHTGDWDDELLQLLRVPRSMLPEVKDSSAHFGDSAYELFGAPIAIRGIA
CCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHCCCEEEEECC
GDQQAATIGQACFSPGMIKSTYGTGCFALLITGATPVKSHNKLLTTIAYQLGGKRTYALE
CCHHHHHHHHHHCCCCCEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEC
GSIFVAGSAVQWLRDGLGIIKHASETGPLADKSDSMQSVYLVPAFVGLGAPYWNPRVRGA
CCEEEECHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCEE
LFGMTRNTGPAELAHAALESVCYQTYDLWAAMRADWSGADAAPPVLRVDGGMAASDWTMQ
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHH
RLADLLDAPVDRPVIQETTALGAAYLAGLSAGVFPEPQKFADNWRLDHRFRPAMSQATRE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
RKLAGWGRAVKGLLASDEGEG
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PFVMAIDQGTTSSRAILFRGDISIAASAQQEFPQHFPASGWVEHEPEDIWASTLATCRA
CEEEEECCCCCCCCEEEEECCCEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AMEKAGATAADIAAIGITNQRETVVVWDAASGKAIHRAIVWQDRRTAEVCTRMKADGYEP
HHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCHHHHEEECCCCHHHHHHHHHCCCCCC
MITDKTGLIIDPYFSGTKVAWLLDNVPGARARAERGELKFGTIDCWLLWRLTGGRVHATD
EEECCCCCEEECCCCCCEEEEEEECCCCCHHHCCCCCEEECEEEEEEEEEECCCEEEECC
ATNASRTLLFNIHTGDWDDELLQLLRVPRSMLPEVKDSSAHFGDSAYELFGAPIAIRGIA
CCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHCCCEEEEECC
GDQQAATIGQACFSPGMIKSTYGTGCFALLITGATPVKSHNKLLTTIAYQLGGKRTYALE
CCHHHHHHHHHHCCCCCEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEC
GSIFVAGSAVQWLRDGLGIIKHASETGPLADKSDSMQSVYLVPAFVGLGAPYWNPRVRGA
CCEEEECHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCEE
LFGMTRNTGPAELAHAALESVCYQTYDLWAAMRADWSGADAAPPVLRVDGGMAASDWTMQ
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHH
RLADLLDAPVDRPVIQETTALGAAYLAGLSAGVFPEPQKFADNWRLDHRFRPAMSQATRE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
RKLAGWGRAVKGLLASDEGEG
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA