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The map label for this gene is metK [H]

Identifier: 86748713

GI number: 86748713

Start: 1789110

End: 1790306

Strand: Direct

Name: metK [H]

Synonym: RPB_1588

Alternate gene names: 86748713

Gene position: 1789110-1790306 (Clockwise)

Preceding gene: 86748712

Following gene: 86748714

Centisome position: 33.56

GC content: 66.5

Gene sequence:

>1197_bases
ATGCGCGCGTCTTATCTGTTCACGAGTGAGTCGGTGTCGGAAGGTCATCCGGACAAGGTCTGCGACCGGATTTCCGACGA
GGTGGTCGATCTGTTCTTCCGCGAGGGGCCGAAAGCCGGCATCGATCCGTGGGCGATTCGGGCGGCGTGCGAGACGCTCG
CCACCACCAACAAGGTGGTGATCGCCGGCGAGACCCGCGGTCCGGCCTCGGTCACCAACGACCAGATCGAGAGCGTCGTC
CGCGCCGCCATCAAGGACATCGGCTACGAGCAGGAAGGCTTCCACTGGGACACCTGCGACATCGAGATCCTGCTGCATCC
GCAATCGGCGGACATCGCGCAGGGCGTCGACGCGCTGCAGCCCGGCACCAACAAGGAAGAGGGCGCCGGCGACCAGGGCA
TCATGTTCGGCTACGCCACCAACGAGACGCCCGATTTGATGCCGGCGCCGATCTTCTACGCCCACAAGATCCTGCGGCTG
ATCTCGGAAGCCCGCCACTCCGGCAAGGAGAAGGTGCTGGGCCCCGACAGCAAGAGCCAGGTCACCATCCAGTACGAGAA
CGGCAAGCCGGTCGGCGTCCGCGAGATCGTGGTGTCGCACCAGCATCTGGTCGAGGACATGACCTCGAACCAGGTCCGCG
AGCGGGTCGAGCCCTATGTCCGCCAGGCGCTGCCGGACGGCTGGATCACCGACAAGACGATCTGGCACATCAATCCCACC
GGCAAGTTCTACATCGGCGGCCCGGACGGCGACACCGGCCTGACCGGCCGCAAGATCATCGTCGACACCTATGGTGGCGC
GGCCCCGCATGGCGGCGGCGCGTTCTCCGGCAAGGACCCGACCAAGGTCGACCGCTCGGCCGCCTATGCGTCGCGCTACC
TCGCCAAGAACATCGTCGCGGCCGGCCTCGCCGACCGCTGCACCCTGCAGCTCGCTTACGCCATCGGCGTGGCGCGGCCG
CTGTCGATCTACATCGACACCCACGGCACCGGGAAGGTTTCGGAAGACAAGCTGGAGAAGGCGGTCGCCGAGGCGATGGA
CCTGACGCCGCGCGGCATCCGCAGCCATCTCGACCTCAACAAGCCGATCTACGCCCGGACCTCGTCCTACGGCCATTTCG
GCCGCACCCCGGACGCCGACGGCGGCTTCTCCTGGGAGAAGACCGATCTTGCCGACGCGCTCAAGCGCGCGGTTTAA

Upstream 100 bases:

>100_bases
TTTACGACGAATCCTACGTCATCTCGCGCCGGCGCGACTGACACTTCAGGGATATCATTCACGAAAACGCCGCCGGGGTC
CGGCGGACAAGGGGTTGGTG

Downstream 100 bases:

>100_bases
CTAGACCGAACAGCCTCTCCCGGTCTTGCCGGGGGAGGTCTCGCCGTCATCCTGAGGTGCGAGCCTCTTCGGCGAGCCTC
GAAGGATGACCCGACGAAGT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MRASYLFTSESVSEGHPDKVCDRISDEVVDLFFREGPKAGIDPWAIRAACETLATTNKVVIAGETRGPASVTNDQIESVV
RAAIKDIGYEQEGFHWDTCDIEILLHPQSADIAQGVDALQPGTNKEEGAGDQGIMFGYATNETPDLMPAPIFYAHKILRL
ISEARHSGKEKVLGPDSKSQVTIQYENGKPVGVREIVVSHQHLVEDMTSNQVRERVEPYVRQALPDGWITDKTIWHINPT
GKFYIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYASRYLAKNIVAAGLADRCTLQLAYAIGVARP
LSIYIDTHGTGKVSEDKLEKAVAEAMDLTPRGIRSHLDLNKPIYARTSSYGHFGRTPDADGGFSWEKTDLADALKRAV

Sequences:

>Translated_398_residues
MRASYLFTSESVSEGHPDKVCDRISDEVVDLFFREGPKAGIDPWAIRAACETLATTNKVVIAGETRGPASVTNDQIESVV
RAAIKDIGYEQEGFHWDTCDIEILLHPQSADIAQGVDALQPGTNKEEGAGDQGIMFGYATNETPDLMPAPIFYAHKILRL
ISEARHSGKEKVLGPDSKSQVTIQYENGKPVGVREIVVSHQHLVEDMTSNQVRERVEPYVRQALPDGWITDKTIWHINPT
GKFYIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYASRYLAKNIVAAGLADRCTLQLAYAIGVARP
LSIYIDTHGTGKVSEDKLEKAVAEAMDLTPRGIRSHLDLNKPIYARTSSYGHFGRTPDADGGFSWEKTDLADALKRAV
>Mature_398_residues
MRASYLFTSESVSEGHPDKVCDRISDEVVDLFFREGPKAGIDPWAIRAACETLATTNKVVIAGETRGPASVTNDQIESVV
RAAIKDIGYEQEGFHWDTCDIEILLHPQSADIAQGVDALQPGTNKEEGAGDQGIMFGYATNETPDLMPAPIFYAHKILRL
ISEARHSGKEKVLGPDSKSQVTIQYENGKPVGVREIVVSHQHLVEDMTSNQVRERVEPYVRQALPDGWITDKTIWHINPT
GKFYIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYASRYLAKNIVAAGLADRCTLQLAYAIGVARP
LSIYIDTHGTGKVSEDKLEKAVAEAMDLTPRGIRSHLDLNKPIYARTSSYGHFGRTPDADGGFSWEKTDLADALKRAV

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=391, Percent_Identity=49.1048593350384, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI4557737, Length=390, Percent_Identity=49.4871794871795, Blast_Score=329, Evalue=2e-90,
Organism=Escherichia coli, GI1789311, Length=398, Percent_Identity=53.7688442211055, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=48.3290488431877, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=48.3290488431877, Blast_Score=330, Evalue=6e-91,
Organism=Caenorhabditis elegans, GI17551082, Length=394, Percent_Identity=46.1928934010152, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=46.4285714285714, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI32565905, Length=387, Percent_Identity=44.702842377261, Blast_Score=290, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI32565911, Length=335, Percent_Identity=47.7611940298507, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI25145635, Length=335, Percent_Identity=45.9701492537313, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=49.1694352159468, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI32565909, Length=86, Percent_Identity=45.3488372093023, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=45.8974358974359, Blast_Score=316, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6323209, Length=394, Percent_Identity=44.1624365482233, Blast_Score=311, Evalue=9e-86,
Organism=Drosophila melanogaster, GI45552159, Length=392, Percent_Identity=48.469387755102, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24580529, Length=392, Percent_Identity=48.469387755102, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24580537, Length=392, Percent_Identity=48.469387755102, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24580533, Length=392, Percent_Identity=48.469387755102, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24580535, Length=392, Percent_Identity=48.469387755102, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24580531, Length=392, Percent_Identity=48.469387755102, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24580527, Length=395, Percent_Identity=48.3544303797468, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24580525, Length=395, Percent_Identity=48.3544303797468, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24580541, Length=394, Percent_Identity=46.1928934010152, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24580539, Length=135, Percent_Identity=41.4814814814815, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43207; Mature: 43207

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASYLFTSESVSEGHPDKVCDRISDEVVDLFFREGPKAGIDPWAIRAACETLATTNKVV
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEE
IAGETRGPASVTNDQIESVVRAAIKDIGYEQEGFHWDTCDIEILLHPQSADIAQGVDALQ
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEEECCCCCHHHHCHHHHC
PGTNKEEGAGDQGIMFGYATNETPDLMPAPIFYAHKILRLISEARHSGKEKVLGPDSKSQ
CCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
VTIQYENGKPVGVREIVVSHQHLVEDMTSNQVRERVEPYVRQALPDGWITDKTIWHINPT
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
GKFYIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYASRYLAKNIVA
CEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AGLADRCTLQLAYAIGVARPLSIYIDTHGTGKVSEDKLEKAVAEAMDLTPRGIRSHLDLN
HCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCC
KPIYARTSSYGHFGRTPDADGGFSWEKTDLADALKRAV
CCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRASYLFTSESVSEGHPDKVCDRISDEVVDLFFREGPKAGIDPWAIRAACETLATTNKVV
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEE
IAGETRGPASVTNDQIESVVRAAIKDIGYEQEGFHWDTCDIEILLHPQSADIAQGVDALQ
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEEECCCCCHHHHCHHHHC
PGTNKEEGAGDQGIMFGYATNETPDLMPAPIFYAHKILRLISEARHSGKEKVLGPDSKSQ
CCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
VTIQYENGKPVGVREIVVSHQHLVEDMTSNQVRERVEPYVRQALPDGWITDKTIWHINPT
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
GKFYIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYASRYLAKNIVA
CEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AGLADRCTLQLAYAIGVARPLSIYIDTHGTGKVSEDKLEKAVAEAMDLTPRGIRSHLDLN
HCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCC
KPIYARTSSYGHFGRTPDADGGFSWEKTDLADALKRAV
CCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA