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The map label for this gene is livJ [C]

Identifier: 86748699

GI number: 86748699

Start: 1772103

End: 1773332

Strand: Reverse

Name: livJ [C]

Synonym: RPB_1574

Alternate gene names: 86748699

Gene position: 1773332-1772103 (Counterclockwise)

Preceding gene: 86748700

Following gene: 86748698

Centisome position: 33.26

GC content: 62.11

Gene sequence:

>1230_bases
ATGAAAAGATTGATCGCTGCCATGGTGATTGCGCTGCCGATGTTCGGCTCCGCGGCGCTGGCCCAGGAAACCGTCAAGAT
CGGCTATATCGATCCGCTGTCCGGCGGCGGCGCCAGCGTCGGCGAAGGCGGCCTCAAGACCTTCCAGTATCTCGCCGACG
AGCTCAACGCCAAGGGCGGCATTCTCGGCAAGAAGGTCGAGATCGTTCCGCTCGACAACAAGACCAACCCGCAGGAAAGC
CTGATCCAGGCGCAGAAGGCGATCGATTCCGGCGTCCGCTACATCACCCAGGGCAACGGTTCCTCGGTCGCCGGCGCGCT
GGCGGACTTCGTCACCAAATACAACGAGCGCAACCCCGGCAAGGAAGTGCTGTACTTCAATTACGCCGCGGTCGACCCGG
TGCTGACCAACGAAAAGTGCAGCTTCTGGCACTTCCGCTGGGACGCGAATTCCGACATCAAGATGGAAGCGCTGACCAAC
TACATGAAGGATCAGCCTTCGATCAAGAAAGTCTATCTGATCAATCAGGACTATTCGTTCGGCCAGTCGGTCCGCACCAC
CGCCCGGGCGATGCTGTCCAAGAAGCGCTCGGACATCCAGATCGTCGGCGACGAACTGCATCCGCTGCTGAAGATCACCG
ACTTCGCCCCCTACATCGCCAAGATCAAGGCGTCCGGCGCCGACACCGTGGTGACCGGCAATTGGGGCCAGGACATCGCG
CTGCTGCTCAAGGCCGCCGCCGATGCCGGCCTCAAGGTGAGCTGGTACACCTATTACGCCGGCGGCGCCGGCGGCCCGAC
TGCGATCAAGCAGACCGGCCTCAACCACCAGGTGTTCCAGATCACCGAAGGCTTCGCCAACGTCGCCCACAAGGAATCGC
AGGACTTCGAAAAGGCGTTCCGCGCCAAGGTCGACCTGAGCCTGTGGTACCCGCGCGCCGTCAACGAAATGCGGATGTTC
GCCGCCGCGGCCGATAAGGCCAAGTCGGTCGATCCGGTCAAGGTCGCTCAGGCGCTCGAGGGCATGAAGTTCGAAGTGTT
CAACGGCGGCCAGGGTGAAATGCGGAAGGACGACCACCAGTTCTTCCAGCCGATCTACGTCTCGTCGTTCGGCGACATCC
CGGCGGGCCAGTTCGACGAAGAGAAGACCGGCTGGGGCTGGAAGCAGGTCGCCAAGATCGACACCGCCCAGACCATGCTG
CCGACCACCTGCAAGATGGACCGGCCGTAA

Upstream 100 bases:

>100_bases
TTTTGCAGAAGCGCAATCGGCGGGTTGAAACTTCCGACCGATCTGCGCTAACACCCTTGCAAGATCAAGAAGCCCCTCAG
GGGATTTAGGGAGAAACAGA

Downstream 100 bases:

>100_bases
CAACGTTTGAAGCGCTGCTCCCCCCTCTCCCCGCTTGCGGGGAGAGGGCTGGGGTGACGGGTCGCCTCCACAAGACGCGG
GCTCCGCGTTCTGTCGAGAC

Product: ABC transporter, periplasmic branched chain amino acid binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKRLIAAMVIALPMFGSAALAQETVKIGYIDPLSGGGASVGEGGLKTFQYLADELNAKGGILGKKVEIVPLDNKTNPQES
LIQAQKAIDSGVRYITQGNGSSVAGALADFVTKYNERNPGKEVLYFNYAAVDPVLTNEKCSFWHFRWDANSDIKMEALTN
YMKDQPSIKKVYLINQDYSFGQSVRTTARAMLSKKRSDIQIVGDELHPLLKITDFAPYIAKIKASGADTVVTGNWGQDIA
LLLKAAADAGLKVSWYTYYAGGAGGPTAIKQTGLNHQVFQITEGFANVAHKESQDFEKAFRAKVDLSLWYPRAVNEMRMF
AAAADKAKSVDPVKVAQALEGMKFEVFNGGQGEMRKDDHQFFQPIYVSSFGDIPAGQFDEEKTGWGWKQVAKIDTAQTML
PTTCKMDRP

Sequences:

>Translated_409_residues
MKRLIAAMVIALPMFGSAALAQETVKIGYIDPLSGGGASVGEGGLKTFQYLADELNAKGGILGKKVEIVPLDNKTNPQES
LIQAQKAIDSGVRYITQGNGSSVAGALADFVTKYNERNPGKEVLYFNYAAVDPVLTNEKCSFWHFRWDANSDIKMEALTN
YMKDQPSIKKVYLINQDYSFGQSVRTTARAMLSKKRSDIQIVGDELHPLLKITDFAPYIAKIKASGADTVVTGNWGQDIA
LLLKAAADAGLKVSWYTYYAGGAGGPTAIKQTGLNHQVFQITEGFANVAHKESQDFEKAFRAKVDLSLWYPRAVNEMRMF
AAAADKAKSVDPVKVAQALEGMKFEVFNGGQGEMRKDDHQFFQPIYVSSFGDIPAGQFDEEKTGWGWKQVAKIDTAQTML
PTTCKMDRP
>Mature_409_residues
MKRLIAAMVIALPMFGSAALAQETVKIGYIDPLSGGGASVGEGGLKTFQYLADELNAKGGILGKKVEIVPLDNKTNPQES
LIQAQKAIDSGVRYITQGNGSSVAGALADFVTKYNERNPGKEVLYFNYAAVDPVLTNEKCSFWHFRWDANSDIKMEALTN
YMKDQPSIKKVYLINQDYSFGQSVRTTARAMLSKKRSDIQIVGDELHPLLKITDFAPYIAKIKASGADTVVTGNWGQDIA
LLLKAAADAGLKVSWYTYYAGGAGGPTAIKQTGLNHQVFQITEGFANVAHKESQDFEKAFRAKVDLSLWYPRAVNEMRMF
AAAADKAKSVDPVKVAQALEGMKFEVFNGGQGEMRKDDHQFFQPIYVSSFGDIPAGQFDEEKTGWGWKQVAKIDTAQTML
PTTCKMDRP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=283, Percent_Identity=23.6749116607774, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 44831; Mature: 44831

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLIAAMVIALPMFGSAALAQETVKIGYIDPLSGGGASVGEGGLKTFQYLADELNAKGG
CHHHHHHHHHHHHCCCCHHHHHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
ILGKKVEIVPLDNKTNPQESLIQAQKAIDSGVRYITQGNGSSVAGALADFVTKYNERNPG
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCC
KEVLYFNYAAVDPVLTNEKCSFWHFRWDANSDIKMEALTNYMKDQPSIKKVYLINQDYSF
CEEEEEEEEECCCEEECCCCCEEEEEECCCCCEEHHHHHHHHHCCCCCEEEEEEECCCCC
GQSVRTTARAMLSKKRSDIQIVGDELHPLLKITDFAPYIAKIKASGADTVVTGNWGQDIA
CHHHHHHHHHHHHCCCCCEEEECCHHCCHHHHHCCCHHHHHEECCCCCEEEECCCCHHHH
LLLKAAADAGLKVSWYTYYAGGAGGPTAIKQTGLNHQVFQITEGFANVAHKESQDFEKAF
HHHHHHHCCCCEEEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RAKVDLSLWYPRAVNEMRMFAAAADKAKSVDPVKVAQALEGMKFEVFNGGQGEMRKDDHQ
HHEECEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCHHH
FFQPIYVSSFGDIPAGQFDEEKTGWGWKQVAKIDTAQTMLPTTCKMDRP
HHCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKRLIAAMVIALPMFGSAALAQETVKIGYIDPLSGGGASVGEGGLKTFQYLADELNAKGG
CHHHHHHHHHHHHCCCCHHHHHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
ILGKKVEIVPLDNKTNPQESLIQAQKAIDSGVRYITQGNGSSVAGALADFVTKYNERNPG
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCC
KEVLYFNYAAVDPVLTNEKCSFWHFRWDANSDIKMEALTNYMKDQPSIKKVYLINQDYSF
CEEEEEEEEECCCEEECCCCCEEEEEECCCCCEEHHHHHHHHHCCCCCEEEEEEECCCCC
GQSVRTTARAMLSKKRSDIQIVGDELHPLLKITDFAPYIAKIKASGADTVVTGNWGQDIA
CHHHHHHHHHHHHCCCCCEEEECCHHCCHHHHHCCCHHHHHEECCCCCEEEECCCCHHHH
LLLKAAADAGLKVSWYTYYAGGAGGPTAIKQTGLNHQVFQITEGFANVAHKESQDFEKAF
HHHHHHHCCCCEEEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RAKVDLSLWYPRAVNEMRMFAAAADKAKSVDPVKVAQALEGMKFEVFNGGQGEMRKDDHQ
HHEECEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCHHH
FFQPIYVSSFGDIPAGQFDEEKTGWGWKQVAKIDTAQTMLPTTCKMDRP
HHCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA