Click here to switch to the map view.

The map label for this gene is rfbD [C]

Identifier: 86748689

GI number: 86748689

Start: 1762856

End: 1763752

Strand: Direct

Name: rfbD [C]

Synonym: RPB_1564

Alternate gene names: 86748689

Gene position: 1762856-1763752 (Clockwise)

Preceding gene: 86748688

Following gene: 86748690

Centisome position: 33.06

GC content: 72.02

Gene sequence:

>897_bases
ATGCGCCTTGCCGTCACAGGCCGTCACGGCCAGGTCGCCCGATCCCTGCTGGAGCGCGCGCCGGCCGATATCGACATCGT
CCCGCTCGCCCGTCCCGAGGTCGACCTGCAGCAGCCCCGCGCCGTCGCCGAGGCGATGGCCGCAGCGCGGCCCGATGCGG
TGGTCAACGCCGCGGCCTACACGGCGGTCGATCTCGCCGAAACCGAGGCCGAGCTGGCTCACCGCATCAACGAAGCCGGC
GCCGGTGCAGTCGCCGCCGCGGCTGCGCGCCTGGGCATTCCGGTGGTGCATCTGTCCACCGACTACGTGTTCGACGGCAG
CCTGTCGCGTCCCTATCGGGAGGACGATGAGACCGGCCCGCTCGGCGTCTATGGCGCCAGCAAGCTCGCCGGCGAACGCG
CGGTGGCGGCCGGCAATCCCGATCACGCGATCCTGCGCACGGCCTGGGTCTACAGCCCGTTCGGCAAGAACTTCGTTCGC
ACCATGCTGACACTCGCCGAGACCCGCGACGAACTCTCTATCGTCTGCGACCAGCGCGGTGCGCCGAGCTACGCGCTCGA
CATCGCGGACGGCATCTTCACGGTGGTTCGCAATCTGCTGGCGCGTCCGGACGACGCGGCGCTACGCGGCGTGTTTCACA
TGACCGGCCGTGGCGACACCGACTGGGCCGGCTTCGCCGAGGCGATCTTTGCCTGCTCGGCCGACGCCGGCGGTCCCGCG
GCGCGGGTGAAGCGGATCGCGACCTCCGACTATCCGACCGCGGCGCGCCGGCCCGCCAATTCCCGCCTCGACACCGCACG
GCTGGCCGCGCGCCACGGCGTGCGGCTGCCGGATTGGCGGGACTCGCTGCCCTCCTGCGTCGACCGGCTGCTGGCGGCGC
GGCGCGTCCACCATTGA

Upstream 100 bases:

>100_bases
CGCGATCGACTGGCCGGTCTCCGCCGACCAGGCGGTGCTGTCGGAGAAGGATATGCAACTGCCGTCGTTCCGGTCTCTCC
CGGCTTACTTCGAGTAGCTC

Downstream 100 bases:

>100_bases
GACAGGCATCGCCATGACCACCGTTTCCAAGGGCATCATTCTCGCAGGCGGCAGTGGGACGCGGCTGTATCCGATGACGG
TGTCGGCCTCTAAGCAGCTG

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MRLAVTGRHGQVARSLLERAPADIDIVPLARPEVDLQQPRAVAEAMAAARPDAVVNAAAYTAVDLAETEAELAHRINEAG
AGAVAAAAARLGIPVVHLSTDYVFDGSLSRPYREDDETGPLGVYGASKLAGERAVAAGNPDHAILRTAWVYSPFGKNFVR
TMLTLAETRDELSIVCDQRGAPSYALDIADGIFTVVRNLLARPDDAALRGVFHMTGRGDTDWAGFAEAIFACSADAGGPA
ARVKRIATSDYPTAARRPANSRLDTARLAARHGVRLPDWRDSLPSCVDRLLAARRVHH

Sequences:

>Translated_298_residues
MRLAVTGRHGQVARSLLERAPADIDIVPLARPEVDLQQPRAVAEAMAAARPDAVVNAAAYTAVDLAETEAELAHRINEAG
AGAVAAAAARLGIPVVHLSTDYVFDGSLSRPYREDDETGPLGVYGASKLAGERAVAAGNPDHAILRTAWVYSPFGKNFVR
TMLTLAETRDELSIVCDQRGAPSYALDIADGIFTVVRNLLARPDDAALRGVFHMTGRGDTDWAGFAEAIFACSADAGGPA
ARVKRIATSDYPTAARRPANSRLDTARLAARHGVRLPDWRDSLPSCVDRLLAARRVHH
>Mature_298_residues
MRLAVTGRHGQVARSLLERAPADIDIVPLARPEVDLQQPRAVAEAMAAARPDAVVNAAAYTAVDLAETEAELAHRINEAG
AGAVAAAAARLGIPVVHLSTDYVFDGSLSRPYREDDETGPLGVYGASKLAGERAVAAGNPDHAILRTAWVYSPFGKNFVR
TMLTLAETRDELSIVCDQRGAPSYALDIADGIFTVVRNLLARPDDAALRGVFHMTGRGDTDWAGFAEAIFACSADAGGPA
ARVKRIATSDYPTAARRPANSRLDTARLAARHGVRLPDWRDSLPSCVDRLLAARRVHH

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=250, Percent_Identity=33.2, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI33519455, Length=250, Percent_Identity=33.2, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1788352, Length=298, Percent_Identity=40.9395973154362, Blast_Score=214, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31774; Mature: 31774

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAVTGRHGQVARSLLERAPADIDIVPLARPEVDLQQPRAVAEAMAAARPDAVVNAAAY
CEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
TAVDLAETEAELAHRINEAGAGAVAAAAARLGIPVVHLSTDYVFDGSLSRPYREDDETGP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCEEECCCCCCCCCCCCCCCC
LGVYGASKLAGERAVAAGNPDHAILRTAWVYSPFGKNFVRTMLTLAETRDELSIVCDQRG
EEECCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCC
APSYALDIADGIFTVVRNLLARPDDAALRGVFHMTGRGDTDWAGFAEAIFACSADAGGPA
CCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCHH
ARVKRIATSDYPTAARRPANSRLDTARLAARHGVRLPDWRDSLPSCVDRLLAARRVHH
HHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLAVTGRHGQVARSLLERAPADIDIVPLARPEVDLQQPRAVAEAMAAARPDAVVNAAAY
CEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
TAVDLAETEAELAHRINEAGAGAVAAAAARLGIPVVHLSTDYVFDGSLSRPYREDDETGP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCEEECCCCCCCCCCCCCCCC
LGVYGASKLAGERAVAAGNPDHAILRTAWVYSPFGKNFVRTMLTLAETRDELSIVCDQRG
EEECCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCC
APSYALDIADGIFTVVRNLLARPDDAALRGVFHMTGRGDTDWAGFAEAIFACSADAGGPA
CCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCHH
ARVKRIATSDYPTAARRPANSRLDTARLAARHGVRLPDWRDSLPSCVDRLLAARRVHH
HHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]