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The map label for this gene is asnB [H]

Identifier: 86748673

GI number: 86748673

Start: 1744455

End: 1746398

Strand: Direct

Name: asnB [H]

Synonym: RPB_1548

Alternate gene names: 86748673

Gene position: 1744455-1746398 (Clockwise)

Preceding gene: 86748672

Following gene: 86748674

Centisome position: 32.72

GC content: 63.07

Gene sequence:

>1944_bases
ATGTGCGGTGTCGCCGGCGTCGCGAGTGCCCGGGGAGTCCGGGTCGAGTTCGAGATCCTGAAGCGGATGACCGATGCGCT
CGCGCATCGCGGCCCGGATGGAGAGGGGCACTGGCTGTCCGAGGACGGCGAGGTCGCGCTCGGGCACCGACGGCTGTCGA
TCATCGATCTGTCCTCGCACGGCGCTCAGCCCATGGTGTCGGCGTCCGGCAGATTCGCGATGTCCTACAACGGCGAAGTC
TACAACTACCTGGAGCTGCGCGACGAGCTGCTGCGCGCCGGCTATCCGTTCCACGGCCAGTCGGACACCGAAGTCATCGT
CGCGGGTTTCGACCATTGGGGAATCGAAAACACCATATCGAGGCTGGCGGGCATGTTCGTGATCGCGGTCTGGGACCGGC
GCGACAAGACCTTGTCGCTGGTCCGCGACCGCATCGGCATCAAGCCGCTGTACTACTCAGTGATCGACGGTGCGCTGGCT
TTCGCATCCGAACTGCGGCCGATCGTTGTTTGGCGAGGCAGCCTTCCGGAGATCTCCCGTCGCGGCCTCTCGGAATTCCT
GCGGCTCGGATACGTTCCCTCGCCACTGAGCATTTTCGAGGGGGTTTTCAAGCTTCCGCCGGCGACGATCGCCACCTTCA
GCGGTGGGCATCTGTCCGAACCGAAGCCTTACTGGTCGGTCGATGCAACGGTTGCACGCGGTTTCGCCAATCTGAGGATG
GACGAGACCGAGGTTCTCGATCAGCTTGATACCCGGTTGGGGGATATCGTCGCCCAGCATATGGTTTCCGATGTTCCGCT
GGGCGCCTTCCTGTCCGGTGGCATCGATTCCAGCGTCGTGACCGCGCTGATGCAGGCCAGAAGCTCGTCGGCCGTCAAGA
CGTTCTCGATCGGTTTCGACAGTGCCGCTTACAATGAGGCCGGCTACGCCGCCGAGGTTGCGGCGCATCTCGGCACAGAT
CACACCGAACTCTACATCACCGAAGCGGATGCGCTGGCGGTGGTGCCGTCGCTTCCCGACATCTACGATGAGCCTTTCGC
AGACATCTCGCAAATTCCGACCTTCCTCGTCGCCGAGCTGGCGCGCCAAAAGGTCACTGTGAGCCTCTCGGGCGACGGCG
GCGACGAATTGTTCGGCGGCTACAATCGCTACATGTTCGTTTCGAACTACTGGAGCCGGCTCAGCAAGATTCCGCTCCCG
CTGCGACGCGTGGCGGCGTCGGCCATCGCCGCGCCGTCGTCGGCCGGCTGGGACAGGATATTCGACCTGCTTGGACCGAT
GTTGCCTGGGAATGCGCGCCCCGCATTGCCTGGACAAAAGATGCAGAAGATCGGTTCGGTGCTGTCGGCGGCCTCCCTGT
CCGAGTTGCAGGGGCGGCTGATCGCCCAATGGCCCCATCCAGAAGCCGTCATGGCGCAGGGCTTTGGTGCAGGCTCGGCG
CTGATGCTGCCCCCGGCGTCGCAAGTTCCCGATCTTCCCGCCGCGGCGCAGCAGATGGCATGGGATACCAGCAGCTATCT
GGTCGATGATATTCTGACGAAGGTCGATCGTGCCACCATGCGGGTGGGTCTCGAGGCTCGCGTCCCGCTATTGGATCACC
GCCTGGTCGAGTTCGCCTGGGAGATTCCGCTGGACATGAAGTTCCGAGACGGGTCCGGCAAGCACATCCTGAAGCGGTTG
CTCGGTCGCTACGTTCCGCGCGCGATGTTCGACCGTCCCAAGATGGGGTTTGGCGTTCCGGTGGACGACTGGCTCAGGGG
GCCTCTCAGGGAATGGGCGGCGAGCTATCTTGCCGCGGCTCGTCACGGCGATGACTATCTCGACCGCGGCGTCATCGCGG
CGACGTGGAAAGAGCATCTGAACGGCAAGATCAACCGCGGCGGACAGCTTTGGACAATCCTGATGTTTGATATCTGGCAC
CAGAGAATTCGCCAATGGGTGTGA

Upstream 100 bases:

>100_bases
AGAGGCACGCGACCGAGGTGCTGGCGTGGTCCGCCATCGCCGCGCAGTTTTCGCCGATATTGAAGACGATCTCGTCGCGC
CGCCGGGCTGGACCGACATA

Downstream 100 bases:

>100_bases
TCTCGCGGTTGCCCTGGTGGGTGAAACTGGGCGCGAAACTCGGGCTGGCGCGCCTTCCCGTCCCCTATGGATTCTGGAAA
CGTATCGGCATTTTCCGCCA

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MCGVAGVASARGVRVEFEILKRMTDALAHRGPDGEGHWLSEDGEVALGHRRLSIIDLSSHGAQPMVSASGRFAMSYNGEV
YNYLELRDELLRAGYPFHGQSDTEVIVAGFDHWGIENTISRLAGMFVIAVWDRRDKTLSLVRDRIGIKPLYYSVIDGALA
FASELRPIVVWRGSLPEISRRGLSEFLRLGYVPSPLSIFEGVFKLPPATIATFSGGHLSEPKPYWSVDATVARGFANLRM
DETEVLDQLDTRLGDIVAQHMVSDVPLGAFLSGGIDSSVVTALMQARSSSAVKTFSIGFDSAAYNEAGYAAEVAAHLGTD
HTELYITEADALAVVPSLPDIYDEPFADISQIPTFLVAELARQKVTVSLSGDGGDELFGGYNRYMFVSNYWSRLSKIPLP
LRRVAASAIAAPSSAGWDRIFDLLGPMLPGNARPALPGQKMQKIGSVLSAASLSELQGRLIAQWPHPEAVMAQGFGAGSA
LMLPPASQVPDLPAAAQQMAWDTSSYLVDDILTKVDRATMRVGLEARVPLLDHRLVEFAWEIPLDMKFRDGSGKHILKRL
LGRYVPRAMFDRPKMGFGVPVDDWLRGPLREWAASYLAAARHGDDYLDRGVIAATWKEHLNGKINRGGQLWTILMFDIWH
QRIRQWV

Sequences:

>Translated_647_residues
MCGVAGVASARGVRVEFEILKRMTDALAHRGPDGEGHWLSEDGEVALGHRRLSIIDLSSHGAQPMVSASGRFAMSYNGEV
YNYLELRDELLRAGYPFHGQSDTEVIVAGFDHWGIENTISRLAGMFVIAVWDRRDKTLSLVRDRIGIKPLYYSVIDGALA
FASELRPIVVWRGSLPEISRRGLSEFLRLGYVPSPLSIFEGVFKLPPATIATFSGGHLSEPKPYWSVDATVARGFANLRM
DETEVLDQLDTRLGDIVAQHMVSDVPLGAFLSGGIDSSVVTALMQARSSSAVKTFSIGFDSAAYNEAGYAAEVAAHLGTD
HTELYITEADALAVVPSLPDIYDEPFADISQIPTFLVAELARQKVTVSLSGDGGDELFGGYNRYMFVSNYWSRLSKIPLP
LRRVAASAIAAPSSAGWDRIFDLLGPMLPGNARPALPGQKMQKIGSVLSAASLSELQGRLIAQWPHPEAVMAQGFGAGSA
LMLPPASQVPDLPAAAQQMAWDTSSYLVDDILTKVDRATMRVGLEARVPLLDHRLVEFAWEIPLDMKFRDGSGKHILKRL
LGRYVPRAMFDRPKMGFGVPVDDWLRGPLREWAASYLAAARHGDDYLDRGVIAATWKEHLNGKINRGGQLWTILMFDIWH
QRIRQWV
>Mature_647_residues
MCGVAGVASARGVRVEFEILKRMTDALAHRGPDGEGHWLSEDGEVALGHRRLSIIDLSSHGAQPMVSASGRFAMSYNGEV
YNYLELRDELLRAGYPFHGQSDTEVIVAGFDHWGIENTISRLAGMFVIAVWDRRDKTLSLVRDRIGIKPLYYSVIDGALA
FASELRPIVVWRGSLPEISRRGLSEFLRLGYVPSPLSIFEGVFKLPPATIATFSGGHLSEPKPYWSVDATVARGFANLRM
DETEVLDQLDTRLGDIVAQHMVSDVPLGAFLSGGIDSSVVTALMQARSSSAVKTFSIGFDSAAYNEAGYAAEVAAHLGTD
HTELYITEADALAVVPSLPDIYDEPFADISQIPTFLVAELARQKVTVSLSGDGGDELFGGYNRYMFVSNYWSRLSKIPLP
LRRVAASAIAAPSSAGWDRIFDLLGPMLPGNARPALPGQKMQKIGSVLSAASLSELQGRLIAQWPHPEAVMAQGFGAGSA
LMLPPASQVPDLPAAAQQMAWDTSSYLVDDILTKVDRATMRVGLEARVPLLDHRLVEFAWEIPLDMKFRDGSGKHILKRL
LGRYVPRAMFDRPKMGFGVPVDDWLRGPLREWAASYLAAARHGDDYLDRGVIAATWKEHLNGKINRGGQLWTILMFDIWH
QRIRQWV

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=397, Percent_Identity=25.9445843828715, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI168229252, Length=397, Percent_Identity=25.9445843828715, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI168229250, Length=397, Percent_Identity=25.9445843828715, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI296010848, Length=371, Percent_Identity=26.4150943396226, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI296010852, Length=300, Percent_Identity=25.6666666666667, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI296010850, Length=300, Percent_Identity=25.6666666666667, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1786889, Length=407, Percent_Identity=31.2039312039312, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71993933, Length=391, Percent_Identity=27.1099744245524, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25147557, Length=391, Percent_Identity=27.1099744245524, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25147560, Length=393, Percent_Identity=25.1908396946565, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17560178, Length=399, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325403, Length=427, Percent_Identity=26.463700234192, Blast_Score=113, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321563, Length=426, Percent_Identity=26.056338028169, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45553209, Length=403, Percent_Identity=29.2803970223325, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71059; Mature: 71059

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVAGVASARGVRVEFEILKRMTDALAHRGPDGEGHWLSEDGEVALGHRRLSIIDLSSH
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCEEEEEEEECCC
GAQPMVSASGRFAMSYNGEVYNYLELRDELLRAGYPFHGQSDTEVIVAGFDHWGIENTIS
CCCCEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHH
RLAGMFVIAVWDRRDKTLSLVRDRIGIKPLYYSVIDGALAFASELRPIVVWRGSLPEISR
HHHHEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
RGLSEFLRLGYVPSPLSIFEGVFKLPPATIATFSGGHLSEPKPYWSVDATVARGFANLRM
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
DETEVLDQLDTRLGDIVAQHMVSDVPLGAFLSGGIDSSVVTALMQARSSSAVKTFSIGFD
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCC
SAAYNEAGYAAEVAAHLGTDHTELYITEADALAVVPSLPDIYDEPFADISQIPTFLVAEL
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCHHCCCHHHHHHHHHHHHHHH
ARQKVTVSLSGDGGDELFGGYNRYMFVSNYWSRLSKIPLPLRRVAASAIAAPSSAGWDRI
HHCEEEEEECCCCCHHHHCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHH
FDLLGPMLPGNARPALPGQKMQKIGSVLSAASLSELQGRLIAQWPHPEAVMAQGFGAGSA
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCCCCCE
LMLPPASQVPDLPAAAQQMAWDTSSYLVDDILTKVDRATMRVGLEARVPLLDHRLVEFAW
EECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EIPLDMKFRDGSGKHILKRLLGRYVPRAMFDRPKMGFGVPVDDWLRGPLREWAASYLAAA
CCCCEEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
RHGDDYLDRGVIAATWKEHLNGKINRGGQLWTILMFDIWHQRIRQWV
HCCCHHHHCCCHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGVAGVASARGVRVEFEILKRMTDALAHRGPDGEGHWLSEDGEVALGHRRLSIIDLSSH
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCEEEEEEEECCC
GAQPMVSASGRFAMSYNGEVYNYLELRDELLRAGYPFHGQSDTEVIVAGFDHWGIENTIS
CCCCEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHH
RLAGMFVIAVWDRRDKTLSLVRDRIGIKPLYYSVIDGALAFASELRPIVVWRGSLPEISR
HHHHEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
RGLSEFLRLGYVPSPLSIFEGVFKLPPATIATFSGGHLSEPKPYWSVDATVARGFANLRM
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
DETEVLDQLDTRLGDIVAQHMVSDVPLGAFLSGGIDSSVVTALMQARSSSAVKTFSIGFD
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCC
SAAYNEAGYAAEVAAHLGTDHTELYITEADALAVVPSLPDIYDEPFADISQIPTFLVAEL
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCHHCCCHHHHHHHHHHHHHHH
ARQKVTVSLSGDGGDELFGGYNRYMFVSNYWSRLSKIPLPLRRVAASAIAAPSSAGWDRI
HHCEEEEEECCCCCHHHHCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHH
FDLLGPMLPGNARPALPGQKMQKIGSVLSAASLSELQGRLIAQWPHPEAVMAQGFGAGSA
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCCCCCE
LMLPPASQVPDLPAAAQQMAWDTSSYLVDDILTKVDRATMRVGLEARVPLLDHRLVEFAW
EECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EIPLDMKFRDGSGKHILKRLLGRYVPRAMFDRPKMGFGVPVDDWLRGPLREWAASYLAAA
CCCCEEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
RHGDDYLDRGVIAATWKEHLNGKINRGGQLWTILMFDIWHQRIRQWV
HCCCHHHHCCCHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]