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The map label for this gene is sdhA [C]

Identifier: 86748603

GI number: 86748603

Start: 1667124

End: 1668899

Strand: Direct

Name: sdhA [C]

Synonym: RPB_1478

Alternate gene names: 86748603

Gene position: 1667124-1668899 (Clockwise)

Preceding gene: 86748602

Following gene: 86748604

Centisome position: 31.27

GC content: 63.4

Gene sequence:

>1776_bases
ATGGAACTGCTGCGAACCCATGAGGAATCGGTGATGGCGATGGACGAAATTCTCGACGACATGTCCGAAGTGTCCTGCGA
CGTGCTGGTGATCGGCGGCGGAACCGCCGGGCCGATGGCGGCGCTGAAGGCCAAGCTGAAGAACCCGAAGGCCAATGTGG
TCCTCCTGGAGAAAGCCAACGTCAAGCGCTCCGGCGCGATCTCGATGGGCATGGACGGGCTCAACAACGCGGTGATCCCC
GGCTACGCCACGCCGGAGCAGTACACCAAGGAGATCACCATCGCGAACGACGGCATCGTCGACCAGAAGGCGGTCTACAA
ATATGCAGAAAATTGCTACTCGATCATCGAGGAGCTCGACTCCTTCGGCATCCGCTTCCTGAAGAACGAGAACGGCGACT
TCGCGGTCAAGAAGGTGCACCATATCGGTACCTATGTGCTGCCGATGCCGAACGGCGAGACCGTCAAGAAGGCGTTGTAC
CGGCAGTTGCGCCGCGCCCGCATCCTGATCTCCAACCGCTACATGGCGACGCGGCTGCTGACCGGCAGCGACGGCCGGGT
CGCCGGCGCCGTCAGCGTCAACACCCGCACGGCCGAGATTCTGGTGATCAAGGCCAAGGCGGTGATCCTGTGCATGGGCG
CTGCCGGCCGGCTCGGGCTGCCGACCTCGGGCTACATGTTCGGCACCTACGAGAACGCCGCGAATTCCGGCGACGGCTAT
GCGATGGCGTATCACGCCGGCGCCGCGCTGGCGAACCTCGAATGCTTCCAGATCAATCCGCTGATCAAGGACTACAACGG
TCCGGCCTGCGCCTATGTGGCCGGGCCTTACGGCGCCTTCACCGCCAACAACGAGGGCGCGCGCTTCATCGAATGCGATT
ACTGGTCCGGCCAGATGATGTTGGAGTTCTTCAACGAGCTGCAGTCCGGCAAGGGCCCGGTATTCCTGAAGCTCAACCAT
CTGCACGAAGACACCATCAGCGAAATCGAGACCACGCTGCACAAGGTCGAGCGGCCGACCCGCGGGCTGTTCCACGAAGG
CCGCGGCACTGATTATCGCCAGCAGCCGATCGAGATGCACATCTCGGAGATCGGGTTCTGCTCCGGCCACAGCGCCTCCG
GCGTGTTCGTCGACGAATTCGCCCGCACCACGATTCCCGGGCTGTACGCCGCCGGCGACATGGCCAGCGTGCCGCACAAC
TACATGCTGGGCGCCTTCACCAACGGCGCGGTGGCCGGCGAGCACGCGATGGAGTTCGCCGACAATGCCGATTTCGCCGC
ATTCGACGCCGCCGACGTCGCGCGCGAGCGAGACCGGGTGATGGCGCCGACCAAGCGCGACGACGGCATTCCGCCGAACC
AGATCGAATACAAGGCGCGGCGGCTGGTGAACGATTATCTGCAGCCACCAAAGGTGACGCGCAAGTTCGAGATCGGCCAG
CGTCGGCTTGCCGAAGTCCGCGACGACATGGAGAACCGGATGATCGCCCGCAACGCGCACGAATTGCTGCGCGCGCTGGA
GACTCAGTCGATCCTGGATTGCGCCGACATGGCGGCGCACGCTTCGCTGTATCGCACCGAAAGCCGCTGGGGCCTCTATC
ATCTCCGCACCGACTATCCGGAGAAGGACGACGACAATTGGTTCTGCCACACGCTGCTCAGCAAGAAGGACGGCAAAATG
GCGAGCGAGAAGCGTGCGGTCGAGCCCTATATCGTTCCGATCGCCGACGACGAGAAGGACCTCTACGACAAGCAGCGCGT
GCGCGCCTCCGCCTGA

Upstream 100 bases:

>100_bases
GGACGCTCGTCCGGGGCAATGGTGGCGCCCTGACAACTAATTGCTATCGCGGTCGGGCCCGCCGTCTATTCTGACAGAAA
TTCCAGTTCTCCGCGGTGGA

Downstream 100 bases:

>100_bases
TCTTTCCAATTTTCCAGCCTGGAGTTCAAAATGCCGCTCGCCAGTTACGAAACCTCGGTTCCCGTCGTGGTCGACGAAGC
CAAATGCATCGCCGACAAGG

Product: putative oxidoreductase

Products: FADH2; Fumarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MELLRTHEESVMAMDEILDDMSEVSCDVLVIGGGTAGPMAALKAKLKNPKANVVLLEKANVKRSGAISMGMDGLNNAVIP
GYATPEQYTKEITIANDGIVDQKAVYKYAENCYSIIEELDSFGIRFLKNENGDFAVKKVHHIGTYVLPMPNGETVKKALY
RQLRRARILISNRYMATRLLTGSDGRVAGAVSVNTRTAEILVIKAKAVILCMGAAGRLGLPTSGYMFGTYENAANSGDGY
AMAYHAGAALANLECFQINPLIKDYNGPACAYVAGPYGAFTANNEGARFIECDYWSGQMMLEFFNELQSGKGPVFLKLNH
LHEDTISEIETTLHKVERPTRGLFHEGRGTDYRQQPIEMHISEIGFCSGHSASGVFVDEFARTTIPGLYAAGDMASVPHN
YMLGAFTNGAVAGEHAMEFADNADFAAFDAADVARERDRVMAPTKRDDGIPPNQIEYKARRLVNDYLQPPKVTRKFEIGQ
RRLAEVRDDMENRMIARNAHELLRALETQSILDCADMAAHASLYRTESRWGLYHLRTDYPEKDDDNWFCHTLLSKKDGKM
ASEKRAVEPYIVPIADDEKDLYDKQRVRASA

Sequences:

>Translated_591_residues
MELLRTHEESVMAMDEILDDMSEVSCDVLVIGGGTAGPMAALKAKLKNPKANVVLLEKANVKRSGAISMGMDGLNNAVIP
GYATPEQYTKEITIANDGIVDQKAVYKYAENCYSIIEELDSFGIRFLKNENGDFAVKKVHHIGTYVLPMPNGETVKKALY
RQLRRARILISNRYMATRLLTGSDGRVAGAVSVNTRTAEILVIKAKAVILCMGAAGRLGLPTSGYMFGTYENAANSGDGY
AMAYHAGAALANLECFQINPLIKDYNGPACAYVAGPYGAFTANNEGARFIECDYWSGQMMLEFFNELQSGKGPVFLKLNH
LHEDTISEIETTLHKVERPTRGLFHEGRGTDYRQQPIEMHISEIGFCSGHSASGVFVDEFARTTIPGLYAAGDMASVPHN
YMLGAFTNGAVAGEHAMEFADNADFAAFDAADVARERDRVMAPTKRDDGIPPNQIEYKARRLVNDYLQPPKVTRKFEIGQ
RRLAEVRDDMENRMIARNAHELLRALETQSILDCADMAAHASLYRTESRWGLYHLRTDYPEKDDDNWFCHTLLSKKDGKM
ASEKRAVEPYIVPIADDEKDLYDKQRVRASA
>Mature_591_residues
MELLRTHEESVMAMDEILDDMSEVSCDVLVIGGGTAGPMAALKAKLKNPKANVVLLEKANVKRSGAISMGMDGLNNAVIP
GYATPEQYTKEITIANDGIVDQKAVYKYAENCYSIIEELDSFGIRFLKNENGDFAVKKVHHIGTYVLPMPNGETVKKALY
RQLRRARILISNRYMATRLLTGSDGRVAGAVSVNTRTAEILVIKAKAVILCMGAAGRLGLPTSGYMFGTYENAANSGDGY
AMAYHAGAALANLECFQINPLIKDYNGPACAYVAGPYGAFTANNEGARFIECDYWSGQMMLEFFNELQSGKGPVFLKLNH
LHEDTISEIETTLHKVERPTRGLFHEGRGTDYRQQPIEMHISEIGFCSGHSASGVFVDEFARTTIPGLYAAGDMASVPHN
YMLGAFTNGAVAGEHAMEFADNADFAAFDAADVARERDRVMAPTKRDDGIPPNQIEYKARRLVNDYLQPPKVTRKFEIGQ
RRLAEVRDDMENRMIARNAHELLRALETQSILDCADMAAHASLYRTESRWGLYHLRTDYPEKDDDNWFCHTLLSKKDGKM
ASEKRAVEPYIVPIADDEKDLYDKQRVRASA

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=532, Percent_Identity=24.2481203007519, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790597, Length=605, Percent_Identity=23.1404958677686, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322701, Length=512, Percent_Identity=25, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322416, Length=509, Percent_Identity=24.950884086444, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1 [C]

Molecular weight: Translated: 65481; Mature: 65481

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLRTHEESVMAMDEILDDMSEVSCDVLVIGGGTAGPMAALKAKLKNPKANVVLLEKAN
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCC
VKRSGAISMGMDGLNNAVIPGYATPEQYTKEITIANDGIVDQKAVYKYAENCYSIIEELD
CCCCCCEEECCCCCCCCCCCCCCCHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
SFGIRFLKNENGDFAVKKVHHIGTYVLPMPNGETVKKALYRQLRRARILISNRYMATRLL
HCCEEEEECCCCCEEHHEEHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
TGSDGRVAGAVSVNTRTAEILVIKAKAVILCMGAAGRLGLPTSGYMFGTYENAANSGDGY
ECCCCCEEEEEEECCCEEEEEEEEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCE
AMAYHAGAALANLECFQINPLIKDYNGPACAYVAGPYGAFTANNEGARFIECDYWSGQMM
EEEEECCCHHHCCEEEEECCHHHCCCCCEEEEEECCCCCEEECCCCCEEEEEECCCCHHH
LEFFNELQSGKGPVFLKLNHLHEDTISEIETTLHKVERPTRGLFHEGRGTDYRQQPIEMH
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHH
ISEIGFCSGHSASGVFVDEFARTTIPGLYAAGDMASVPHNYMLGAFTNGAVAGEHAMEFA
HHHHCCCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCCCCCEEEECCCCCCCCHHHHHHC
DNADFAAFDAADVARERDRVMAPTKRDDGIPPNQIEYKARRLVNDYLQPPKVTRKFEIGQ
CCCCCEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
RRLAEVRDDMENRMIARNAHELLRALETQSILDCADMAAHASLYRTESRWGLYHLRTDYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCC
EKDDDNWFCHTLLSKKDGKMASEKRAVEPYIVPIADDEKDLYDKQRVRASA
CCCCCCEEEHHHHCCCCCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MELLRTHEESVMAMDEILDDMSEVSCDVLVIGGGTAGPMAALKAKLKNPKANVVLLEKAN
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCC
VKRSGAISMGMDGLNNAVIPGYATPEQYTKEITIANDGIVDQKAVYKYAENCYSIIEELD
CCCCCCEEECCCCCCCCCCCCCCCHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
SFGIRFLKNENGDFAVKKVHHIGTYVLPMPNGETVKKALYRQLRRARILISNRYMATRLL
HCCEEEEECCCCCEEHHEEHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
TGSDGRVAGAVSVNTRTAEILVIKAKAVILCMGAAGRLGLPTSGYMFGTYENAANSGDGY
ECCCCCEEEEEEECCCEEEEEEEEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCE
AMAYHAGAALANLECFQINPLIKDYNGPACAYVAGPYGAFTANNEGARFIECDYWSGQMM
EEEEECCCHHHCCEEEEECCHHHCCCCCEEEEEECCCCCEEECCCCCEEEEEECCCCHHH
LEFFNELQSGKGPVFLKLNHLHEDTISEIETTLHKVERPTRGLFHEGRGTDYRQQPIEMH
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHH
ISEIGFCSGHSASGVFVDEFARTTIPGLYAAGDMASVPHNYMLGAFTNGAVAGEHAMEFA
HHHHCCCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCCCCCEEEECCCCCCCCHHHHHHC
DNADFAAFDAADVARERDRVMAPTKRDDGIPPNQIEYKARRLVNDYLQPPKVTRKFEIGQ
CCCCCEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
RRLAEVRDDMENRMIARNAHELLRALETQSILDCADMAAHASLYRTESRWGLYHLRTDYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCC
EKDDDNWFCHTLLSKKDGKMASEKRAVEPYIVPIADDEKDLYDKQRVRASA
CCCCCCEEEHHHHCCCCCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: FAD; Succinate [C]

Specific reaction: FAD + Succinate --> FADH2 + Fumarate [C]

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]