Click here to switch to the map view.

The map label for this gene is potH [H]

Identifier: 86748580

GI number: 86748580

Start: 1643800

End: 1644717

Strand: Direct

Name: potH [H]

Synonym: RPB_1455

Alternate gene names: 86748580

Gene position: 1643800-1644717 (Clockwise)

Preceding gene: 86748579

Following gene: 86748581

Centisome position: 30.83

GC content: 66.01

Gene sequence:

>918_bases
ATGAGCGGCGCACGCCGGATCTTCACGCGCCCGGCGCGGCTTGCGGTGATCGCGCCGTATCTGTGGCTGGTGCTGTTCTT
CCTGGTGCCGTTCGGCTTCGTGGTGAAGATCAGCCTGTCGCAGAGCGCGATCGCGCAGCCGCCTTACGTTCCGGTGTTCG
ATCTCGCCGCCGGCTGGGACGCGCTCGCCGCGGCGTTCGCGGCGCTGTCGCTGGACAATTTCCGGCTGCTGATCTCCGAC
AATCTCTATGTGCTGTCCTATCTGCGCAGCCTCACCGTCGCCGCGGTCTCGACCGCGATCCTGTTGCTGATCGGCTATCC
GATCGCCTATGCGATGGCGCGACTGCCGCGCGGCTGGCAGCCCTTCGCGATGATGCTGGTGATCGTGCCGTTCTGGACCT
CGTTCCTGATCCGGATCTACGCCTGGATCAACATCCTGCAGCACGACGGCCTGCTCAATCAGACGCTGCTGGCGCTGCAT
CTGGTGTCGGCGCCGCTGGTGTGGCTGTCGACCGACACCGCGATGTATATCGGCATCGTGTATTCCTACCTGCCGTTCAT
GGTGCTGCCGTTGTATGCGACGTTGGCGAAGCTGCCGCAGGCGCTGAACGAGGCCGCCGCCGACCTCGGCTGTTCGCCGC
GCCGGGCGTTCTGGCTGGTGACGTTTCCGCTGTCGCTGCCCGGCATCGGCGCCGGCGTGCTGCTGTGCTTCATCCCGATC
GTCGGCGAGTTCGTGATTCCGGATCTCTTGGCCGGCTCCGCGTCGCTGATGATCGGGCAGACGCTGTGGCTGGAGTTCTT
CACCAACCGCGACTGGCCGGTGGCCTCGGCGATCGCGGTGGTGCTGCTCGGCGTGCTGTTGCTGCCGCTCCTGATCTACG
AGCGGCTGCAGCGGCGGCAGCTCGAAGGAGAGCGGTGA

Upstream 100 bases:

>100_bases
CGAAGATGGCCAATACCACGCGGCTCGACGTCGACGCCTATCGCGCCGGCGAGCACGTGGTGGCGTGGTTCTCGCCCGAC
GATTGCGTGGTGCTCGACCG

Downstream 100 bases:

>100_bases
TGGCCGCGCGCGCCGCCCGCCTGTCCCGCTTCAACCTCGTGTCGCTGACGCTGGGACTTGCGTTCCTGTATCTGCCGATC
GTCATCATGGTGATCTACTC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSGARRIFTRPARLAVIAPYLWLVLFFLVPFGFVVKISLSQSAIAQPPYVPVFDLAAGWDALAAAFAALSLDNFRLLISD
NLYVLSYLRSLTVAAVSTAILLLIGYPIAYAMARLPRGWQPFAMMLVIVPFWTSFLIRIYAWINILQHDGLLNQTLLALH
LVSAPLVWLSTDTAMYIGIVYSYLPFMVLPLYATLAKLPQALNEAAADLGCSPRRAFWLVTFPLSLPGIGAGVLLCFIPI
VGEFVIPDLLAGSASLMIGQTLWLEFFTNRDWPVASAIAVVLLGVLLLPLLIYERLQRRQLEGER

Sequences:

>Translated_305_residues
MSGARRIFTRPARLAVIAPYLWLVLFFLVPFGFVVKISLSQSAIAQPPYVPVFDLAAGWDALAAAFAALSLDNFRLLISD
NLYVLSYLRSLTVAAVSTAILLLIGYPIAYAMARLPRGWQPFAMMLVIVPFWTSFLIRIYAWINILQHDGLLNQTLLALH
LVSAPLVWLSTDTAMYIGIVYSYLPFMVLPLYATLAKLPQALNEAAADLGCSPRRAFWLVTFPLSLPGIGAGVLLCFIPI
VGEFVIPDLLAGSASLMIGQTLWLEFFTNRDWPVASAIAVVLLGVLLLPLLIYERLQRRQLEGER
>Mature_304_residues
SGARRIFTRPARLAVIAPYLWLVLFFLVPFGFVVKISLSQSAIAQPPYVPVFDLAAGWDALAAAFAALSLDNFRLLISDN
LYVLSYLRSLTVAAVSTAILLLIGYPIAYAMARLPRGWQPFAMMLVIVPFWTSFLIRIYAWINILQHDGLLNQTLLALHL
VSAPLVWLSTDTAMYIGIVYSYLPFMVLPLYATLAKLPQALNEAAADLGCSPRRAFWLVTFPLSLPGIGAGVLLCFIPIV
GEFVIPDLLAGSASLMIGQTLWLEFFTNRDWPVASAIAVVLLGVLLLPLLIYERLQRRQLEGER

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=293, Percent_Identity=49.4880546075085, Blast_Score=247, Evalue=6e-67,
Organism=Escherichia coli, GI1787369, Length=246, Percent_Identity=41.4634146341463, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1787713, Length=240, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33756; Mature: 33625

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGARRIFTRPARLAVIAPYLWLVLFFLVPFGFVVKISLSQSAIAQPPYVPVFDLAAGWD
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHCCCCCCCHHHHHCCHH
ALAAAFAALSLDNFRLLISDNLYVLSYLRSLTVAAVSTAILLLIGYPIAYAMARLPRGWQ
HHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFAMMLVIVPFWTSFLIRIYAWINILQHDGLLNQTLLALHLVSAPLVWLSTDTAMYIGIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHH
YSYLPFMVLPLYATLAKLPQALNEAAADLGCSPRRAFWLVTFPLSLPGIGAGVLLCFIPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
VGEFVIPDLLAGSASLMIGQTLWLEFFTNRDWPVASAIAVVLLGVLLLPLLIYERLQRRQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEGER
CCCCC
>Mature Secondary Structure 
SGARRIFTRPARLAVIAPYLWLVLFFLVPFGFVVKISLSQSAIAQPPYVPVFDLAAGWD
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHCCCCCCCHHHHHCCHH
ALAAAFAALSLDNFRLLISDNLYVLSYLRSLTVAAVSTAILLLIGYPIAYAMARLPRGWQ
HHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFAMMLVIVPFWTSFLIRIYAWINILQHDGLLNQTLLALHLVSAPLVWLSTDTAMYIGIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHH
YSYLPFMVLPLYATLAKLPQALNEAAADLGCSPRRAFWLVTFPLSLPGIGAGVLLCFIPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
VGEFVIPDLLAGSASLMIGQTLWLEFFTNRDWPVASAIAVVLLGVLLLPLLIYERLQRRQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEGER
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]