Click here to switch to the map view.

The map label for this gene is purK [H]

Identifier: 86748571

GI number: 86748571

Start: 1633396

End: 1634490

Strand: Reverse

Name: purK [H]

Synonym: RPB_1446

Alternate gene names: 86748571

Gene position: 1634490-1633396 (Counterclockwise)

Preceding gene: 86748572

Following gene: 86748570

Centisome position: 30.66

GC content: 68.58

Gene sequence:

>1095_bases
GTGACCGGTCCCGCTCGGCAAGTGCTCAAACCCGGCGACACCATCGGCATTCTCGGCGGCGGCCAGCTCGGCCGGATGCT
GGCGATGGCCGCAGCAAGGCTCGGCCTGCGCTGCAATGTGTTCTCGCCGGACCCGGATTCGCCGGCCTTCGACGTGGTGC
AGAACGCCGTCTGCGCCGAATATGCCGATGTCGAGGCGCTGGAGATGTTCGCCGCCGACGTCGACGTCATCACCTATGAA
TTCGAGAACGTGCCGGCCTCGGCGGCGCTGGTGCTGGCGGCGCGCAAGCCGGTGCTGCCGGACTACAAGATCCTGGAGAC
CACCCAGGATCGCCTCGCCGAGAAGGATTTCGTCACCGGCCTCGGCATCGGCACCGCCGCCTATGCCGACGTCACCTCGG
CGCAGACGCTACGCGCCGCGATCGCCAAGCTCGGCCTGCCCGCAGTGCTGAAGACGCGGCGGTTCGGCTATGACGGCAAG
GGCCAGGTGATCATCCGCGAGGGCGACGATCCCGATGCGGCCTGGGAGAAGCTGGAGACCCGCGCGGCGATTCTCGAGGC
CTTCGTGCCGTTCGAGCGCGAAGTCTCGGTGATCGCCGCGCGCGGCGCCGACGGCCAGGTGGTGTGCTACGATGTCACCG
AGAACGAGCACCGCGACCACATCCTCAAAGTGTCGCGGGTGCCGGCGCCGGTGAGCGACTCCGTCGCCGGCGAGGCACGG
CGGATCGCCACCAGCATCGCCGATGCGCTGAACTATGTCGGCGTGCTGGCGGTCGAGATGTTCGTGGTGCCGGGCGACGG
CGGCGCGACCGTGCTGGTCAACGAGATCGCGCCCCGGGTGCACAATTCCGGGCACTGGACGCTCGACGGCGCCTCGGTGT
CGCAGTTCGAGCAGCACATCCGGGCGATCGCCGGCTGGCCGCTGGCGGAACCGCTACGCCACGGCGCCGTCACCATGACC
AACCTGATCGGCCACGATGTCGACGATTATGCCCGCTGGCTGACGGTTCCCGGCGCCACGGTGCATCTCTACGGCAAGCG
GACGGCTTTGCCGGGCCGTAAGATGGGCCACGTCACCGTGATCGAGCCACGATGA

Upstream 100 bases:

>100_bases
CGAGCGTGCTGGCGCTGACCGACGAGGCGCTGGCGGCACGGCTGAGCGCCTGGCGCAAACAACAGACCGATGGAATTGCC
GCCCGGCCGGAGGGCGCGGC

Downstream 100 bases:

>100_bases
TCGAGCGCGATGATCGAACCGGCCGGAAACGCTGCGCCACGGCCGACCAATGTCGCAAAAACTTATCCTCAACCGAGGTG
GACAGCGGCGGCCCGATCTG

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MTGPARQVLKPGDTIGILGGGQLGRMLAMAAARLGLRCNVFSPDPDSPAFDVVQNAVCAEYADVEALEMFAADVDVITYE
FENVPASAALVLAARKPVLPDYKILETTQDRLAEKDFVTGLGIGTAAYADVTSAQTLRAAIAKLGLPAVLKTRRFGYDGK
GQVIIREGDDPDAAWEKLETRAAILEAFVPFEREVSVIAARGADGQVVCYDVTENEHRDHILKVSRVPAPVSDSVAGEAR
RIATSIADALNYVGVLAVEMFVVPGDGGATVLVNEIAPRVHNSGHWTLDGASVSQFEQHIRAIAGWPLAEPLRHGAVTMT
NLIGHDVDDYARWLTVPGATVHLYGKRTALPGRKMGHVTVIEPR

Sequences:

>Translated_364_residues
MTGPARQVLKPGDTIGILGGGQLGRMLAMAAARLGLRCNVFSPDPDSPAFDVVQNAVCAEYADVEALEMFAADVDVITYE
FENVPASAALVLAARKPVLPDYKILETTQDRLAEKDFVTGLGIGTAAYADVTSAQTLRAAIAKLGLPAVLKTRRFGYDGK
GQVIIREGDDPDAAWEKLETRAAILEAFVPFEREVSVIAARGADGQVVCYDVTENEHRDHILKVSRVPAPVSDSVAGEAR
RIATSIADALNYVGVLAVEMFVVPGDGGATVLVNEIAPRVHNSGHWTLDGASVSQFEQHIRAIAGWPLAEPLRHGAVTMT
NLIGHDVDDYARWLTVPGATVHLYGKRTALPGRKMGHVTVIEPR
>Mature_363_residues
TGPARQVLKPGDTIGILGGGQLGRMLAMAAARLGLRCNVFSPDPDSPAFDVVQNAVCAEYADVEALEMFAADVDVITYEF
ENVPASAALVLAARKPVLPDYKILETTQDRLAEKDFVTGLGIGTAAYADVTSAQTLRAAIAKLGLPAVLKTRRFGYDGKG
QVIIREGDDPDAAWEKLETRAAILEAFVPFEREVSVIAARGADGQVVCYDVTENEHRDHILKVSRVPAPVSDSVAGEARR
IATSIADALNYVGVLAVEMFVVPGDGGATVLVNEIAPRVHNSGHWTLDGASVSQFEQHIRAIAGWPLAEPLRHGAVTMTN
LIGHDVDDYARWLTVPGATVHLYGKRTALPGRKMGHVTVIEPR

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=351, Percent_Identity=35.8974358974359, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788155, Length=365, Percent_Identity=26.5753424657534, Blast_Score=98, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=33.0601092896175, Blast_Score=201, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 38937; Mature: 38806

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPARQVLKPGDTIGILGGGQLGRMLAMAAARLGLRCNVFSPDPDSPAFDVVQNAVCAE
CCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHH
YADVEALEMFAADVDVITYEFENVPASAALVLAARKPVLPDYKILETTQDRLAEKDFVTG
HCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LGIGTAAYADVTSAQTLRAAIAKLGLPAVLKTRRFGYDGKGQVIIREGDDPDAAWEKLET
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHH
RAAILEAFVPFEREVSVIAARGADGQVVCYDVTENEHRDHILKVSRVPAPVSDSVAGEAR
HHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHEEEEECCCCCCCCCHHHHHH
RIATSIADALNYVGVLAVEMFVVPGDGGATVLVNEIAPRVHNSGHWTLDGASVSQFEQHI
HHHHHHHHHHHHHHEEEEEEEEEECCCCCEEEEEHHHHHHCCCCCEEECCCCHHHHHHHH
RAIAGWPLAEPLRHGAVTMTNLIGHDVDDYARWLTVPGATVHLYGKRTALPGRKMGHVTV
HHHHCCCCHHHHHCCCEEEHHHHCCCHHHHHHEEECCCCEEEEEECCCCCCCCCCCCEEE
IEPR
ECCC
>Mature Secondary Structure 
TGPARQVLKPGDTIGILGGGQLGRMLAMAAARLGLRCNVFSPDPDSPAFDVVQNAVCAE
CCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHH
YADVEALEMFAADVDVITYEFENVPASAALVLAARKPVLPDYKILETTQDRLAEKDFVTG
HCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LGIGTAAYADVTSAQTLRAAIAKLGLPAVLKTRRFGYDGKGQVIIREGDDPDAAWEKLET
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHH
RAAILEAFVPFEREVSVIAARGADGQVVCYDVTENEHRDHILKVSRVPAPVSDSVAGEAR
HHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHEEEEECCCCCCCCCHHHHHH
RIATSIADALNYVGVLAVEMFVVPGDGGATVLVNEIAPRVHNSGHWTLDGASVSQFEQHI
HHHHHHHHHHHHHHEEEEEEEEEECCCCCEEEEEHHHHHHCCCCCEEECCCCHHHHHHHH
RAIAGWPLAEPLRHGAVTMTNLIGHDVDDYARWLTVPGATVHLYGKRTALPGRKMGHVTV
HHHHCCCCHHHHHCCCEEEHHHHCCCHHHHHHEEECCCCEEEEEECCCCCCCCCCCCEEE
IEPR
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]