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The map label for this gene is mhpC2 [H]

Identifier: 86748545

GI number: 86748545

Start: 1608246

End: 1609208

Strand: Direct

Name: mhpC2 [H]

Synonym: RPB_1420

Alternate gene names: 86748545

Gene position: 1608246-1609208 (Clockwise)

Preceding gene: 86748544

Following gene: 86748546

Centisome position: 30.16

GC content: 68.74

Gene sequence:

>963_bases
ATGATCGCAATCGGCGCTGTCGCCATGCTCGCCGCGCTGGCGCTGCTCACGCAGGCCGGCGTGCTGTGGCTGCAGGGCAA
ATTTCCGCCGCAGGGCGAGCGGATTGCCGTCAGCGGCGGCAAGCTGCACGTCGTCGATCTCGGCCCCCGCAACCTCGCCA
CGTTGCCGATCGTGATGATCCACGGCGCCAGCTCCAATCTCGGCGCGATGCGCGTGCCGCTCGGCGATGCGCTCGCGCTG
CGGCACCGCGTTCTGCTGATCGATCGGCCCGGCCATGGCTGGAGCAGCCGCGAAGACCTCGCCAATTCCTCGCCCGCGAT
GCAGGCGGAAGCGATCGGCGAGGCGCTCGACGCCCTCGGCGTGTCGCGCGCGATTGTCGTCGTGCATTCGATGGCGGGCG
CACTCGGCGCGCGGCTGGCGCTGGATCAGCCGCAACGTGTTGCGGGGCTGGTCATGCTGGCGCCGGTGACGCATCCGTGG
CGCGGCGGCGTCGGGACCTACAACAGGGTGATGACCACGCCGGTGCTCGGTCCGCTATTGGCCCACACCATCACGCTACC
GCTCGGATCGCTACTCACCGAGCCCGGGGCGCGTTCGGCATTCACACCGCAGACCATGCCGGAGGACTACGTCGACGCCA
GCGCGACTCCGCTGCTGCTGCGACCGCGCGCATTCCTTGCCAATAGCTGGGACTTGACCACGCTGAAGGAGGCCGTGGCC
GAGCAGGCCCCGCGCTACCCGCAGATCACTGCGCCGACCGTGCTGATCCATGGCGACGCCGACGAGATCGTCTCTGTCGA
CATCCATTCGCGCGCCTTCGCCGCCGCGGTTCCGCAGGCGAAGCTGATCGTGCTGCCGGACGTCGGGCACATGGTGCAGA
ATACCGCCCCCGATCTGGTCCAGCGCGAAGTCGAGGCGCTGATTGCAACGGCCGTCACGAAATCGCACGCAGCGATGCAC
TAG

Upstream 100 bases:

>100_bases
ACGGAAAAGGTCGAGGGCCGCGATATCTGAGCGGGACGACGAAACATTCGCGCGCGCACCGCGTAACTCACCCGTGCGAA
AAATCTCCCGGGTCCCGTCC

Downstream 100 bases:

>100_bases
ACTTTCATCCCCGACTACTCACCAAAAGCAGGAGCTGCACATGAGCACGACATTCGGTTCGGCCAAATGGCAGGGCGGCA
TCAAGGACGGCAAGGGCGCG

Product: alpha/beta hydrolase fold

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase 2; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase 2; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase 2 [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIAIGAVAMLAALALLTQAGVLWLQGKFPPQGERIAVSGGKLHVVDLGPRNLATLPIVMIHGASSNLGAMRVPLGDALAL
RHRVLLIDRPGHGWSSREDLANSSPAMQAEAIGEALDALGVSRAIVVVHSMAGALGARLALDQPQRVAGLVMLAPVTHPW
RGGVGTYNRVMTTPVLGPLLAHTITLPLGSLLTEPGARSAFTPQTMPEDYVDASATPLLLRPRAFLANSWDLTTLKEAVA
EQAPRYPQITAPTVLIHGDADEIVSVDIHSRAFAAAVPQAKLIVLPDVGHMVQNTAPDLVQREVEALIATAVTKSHAAMH

Sequences:

>Translated_320_residues
MIAIGAVAMLAALALLTQAGVLWLQGKFPPQGERIAVSGGKLHVVDLGPRNLATLPIVMIHGASSNLGAMRVPLGDALAL
RHRVLLIDRPGHGWSSREDLANSSPAMQAEAIGEALDALGVSRAIVVVHSMAGALGARLALDQPQRVAGLVMLAPVTHPW
RGGVGTYNRVMTTPVLGPLLAHTITLPLGSLLTEPGARSAFTPQTMPEDYVDASATPLLLRPRAFLANSWDLTTLKEAVA
EQAPRYPQITAPTVLIHGDADEIVSVDIHSRAFAAAVPQAKLIVLPDVGHMVQNTAPDLVQREVEALIATAVTKSHAAMH
>Mature_320_residues
MIAIGAVAMLAALALLTQAGVLWLQGKFPPQGERIAVSGGKLHVVDLGPRNLATLPIVMIHGASSNLGAMRVPLGDALAL
RHRVLLIDRPGHGWSSREDLANSSPAMQAEAIGEALDALGVSRAIVVVHSMAGALGARLALDQPQRVAGLVMLAPVTHPW
RGGVGTYNRVMTTPVLGPLLAHTITLPLGSLLTEPGARSAFTPQTMPEDYVDASATPLLLRPRAFLANSWDLTTLKEAVA
EQAPRYPQITAPTVLIHGDADEIVSVDIHSRAFAAAVPQAKLIVLPDVGHMVQNTAPDLVQREVEALIATAVTKSHAAMH

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MhpC family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33615; Mature: 33615

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIGAVAMLAALALLTQAGVLWLQGKFPPQGERIAVSGGKLHVVDLGPRNLATLPIVMI
CEEHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCCCEEEEEEE
HGASSNLGAMRVPLGDALALRHRVLLIDRPGHGWSSREDLANSSPAMQAEAIGEALDALG
ECCCCCCCEEECCCCHHHHHCEEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHH
VSRAIVVVHSMAGALGARLALDQPQRVAGLVMLAPVTHPWRGGVGTYNRVMTTPVLGPLL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
AHTITLPLGSLLTEPGARSAFTPQTMPEDYVDASATPLLLRPRAFLANSWDLTTLKEAVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCEEECCHHHHCCCCCHHHHHHHHH
EQAPRYPQITAPTVLIHGDADEIVSVDIHSRAFAAAVPQAKLIVLPDVGHMVQNTAPDLV
HHCCCCCCCCCCEEEEECCCCCEEEEEECCHHHHHHCCCCEEEEECCCHHHHHCCCHHHH
QREVEALIATAVTKSHAAMH
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIAIGAVAMLAALALLTQAGVLWLQGKFPPQGERIAVSGGKLHVVDLGPRNLATLPIVMI
CEEHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCCCEEEEEEE
HGASSNLGAMRVPLGDALALRHRVLLIDRPGHGWSSREDLANSSPAMQAEAIGEALDALG
ECCCCCCCEEECCCCHHHHHCEEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHH
VSRAIVVVHSMAGALGARLALDQPQRVAGLVMLAPVTHPWRGGVGTYNRVMTTPVLGPLL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
AHTITLPLGSLLTEPGARSAFTPQTMPEDYVDASATPLLLRPRAFLANSWDLTTLKEAVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCEEECCHHHHCCCCCHHHHHHHHH
EQAPRYPQITAPTVLIHGDADEIVSVDIHSRAFAAAVPQAKLIVLPDVGHMVQNTAPDLV
HHCCCCCCCCCCEEEEECCCCCEEEEEECCHHHHHHCCCCEEEEECCCHHHHHCCCHHHH
QREVEALIATAVTKSHAAMH
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA