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The map label for this gene is glsA

Identifier: 86748528

GI number: 86748528

Start: 1594676

End: 1595611

Strand: Direct

Name: glsA

Synonym: RPB_1403

Alternate gene names: 86748528

Gene position: 1594676-1595611 (Clockwise)

Preceding gene: 86748527

Following gene: 86748530

Centisome position: 29.91

GC content: 68.38

Gene sequence:

>936_bases
ATGAACCCTGACCTGCTCGACCACACCGTCGCCGAGATCGCCGCAGAGATGGCGCAGCGGCCCGACCGCGGCGCGGTCGC
GAGCTACATCCCCGAACTGGCTGGCGTCGATCCGCAGCGCTTCGGCCTCGTCGTGATCGACGCCGACGGCCATGTCGCGG
CCGGTGGCGATGCCGATATGCCGTTCTCGATCCAGAGCATCTCCAAGGTGTTCACGCTGACGCTGGCGCTCGGCCTGGCC
GGCGACCGGGTGTGGCGCCGGGTCGGCCGCGAGCCGTCGGGCTCGGCCTTCAATTCCATCGTTCAACTCGAGCGCGAGCG
CGGCATCCCGCGCAATCCCTTCATCAACGCCGGCGCGATCGCGGTCACCGACCTGATCCTGTCGGGCCACCAGCCGCGCG
AGGCGCTCGGCGAGATTCTGCGCTTCATGCAGTTCCTGGCCGGCGACGACAGCATCGTGATCGACGACGCCGTGGCCGCC
TCGGAGCAGCGCACCGGCTTCCGCAACGCGGCGCTGGCGAACTACATGAAGTCCTTCGGCGTGCTCGACAATCCGGTCGA
GTACACGCTCGGCGTCTATTTCCATCATTGCGCGATTGCGATGAGCTGCCGCCAGTTGGCGCTGGCGGGGCGCTTTCTCG
CCCACAACGGCCGCAATCCGTCGACCGGCCACAACGTGGTGTCGACCCAGCGGGCGCGACGCATCAATGCGCTGATGCTG
ACTTGCGGGCACTATGACGGCTCGGGCGAATTCGCCTATCGGGTGGGGCTACCCGGCAAAAGTGGAGTCGGGGGAGGGGT
GCTGGCGGTCGCGCCCGGCAAGGCCTCGATCGCGGTGTGGTCGCCGGGGCTCGATGCGGCGGGCAATTCGCATCTCGGCC
GGATCGCGCTCGAGGCGCTGACCAGGCGGCTGGGCTGGTCGATCTTCGGGGTTTGA

Upstream 100 bases:

>100_bases
TGCCACGCGCGGGGGGCGTACTCGTAATGAAGGCCTGCATCCCCCTCTGCATCCTCTTTTACCTTGCTTTGCGGCGGGGC
TTGCCGTTCTTTCCGCCACC

Downstream 100 bases:

>100_bases
CGGACTCCGGCGCCGAGGCGCGCGTCGCCCGACAAAAGCGAAGGGCCGAACGTCTGAACGCCGGCCCTTCGTCAGGTCTG
GACTGTCGCGCCGCGCGGCG

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNPDLLDHTVAEIAAEMAQRPDRGAVASYIPELAGVDPQRFGLVVIDADGHVAAGGDADMPFSIQSISKVFTLTLALGLA
GDRVWRRVGREPSGSAFNSIVQLERERGIPRNPFINAGAIAVTDLILSGHQPREALGEILRFMQFLAGDDSIVIDDAVAA
SEQRTGFRNAALANYMKSFGVLDNPVEYTLGVYFHHCAIAMSCRQLALAGRFLAHNGRNPSTGHNVVSTQRARRINALML
TCGHYDGSGEFAYRVGLPGKSGVGGGVLAVAPGKASIAVWSPGLDAAGNSHLGRIALEALTRRLGWSIFGV

Sequences:

>Translated_311_residues
MNPDLLDHTVAEIAAEMAQRPDRGAVASYIPELAGVDPQRFGLVVIDADGHVAAGGDADMPFSIQSISKVFTLTLALGLA
GDRVWRRVGREPSGSAFNSIVQLERERGIPRNPFINAGAIAVTDLILSGHQPREALGEILRFMQFLAGDDSIVIDDAVAA
SEQRTGFRNAALANYMKSFGVLDNPVEYTLGVYFHHCAIAMSCRQLALAGRFLAHNGRNPSTGHNVVSTQRARRINALML
TCGHYDGSGEFAYRVGLPGKSGVGGGVLAVAPGKASIAVWSPGLDAAGNSHLGRIALEALTRRLGWSIFGV
>Mature_311_residues
MNPDLLDHTVAEIAAEMAQRPDRGAVASYIPELAGVDPQRFGLVVIDADGHVAAGGDADMPFSIQSISKVFTLTLALGLA
GDRVWRRVGREPSGSAFNSIVQLERERGIPRNPFINAGAIAVTDLILSGHQPREALGEILRFMQFLAGDDSIVIDDAVAA
SEQRTGFRNAALANYMKSFGVLDNPVEYTLGVYFHHCAIAMSCRQLALAGRFLAHNGRNPSTGHNVVSTQRARRINALML
TCGHYDGSGEFAYRVGLPGKSGVGGGVLAVAPGKASIAVWSPGLDAAGNSHLGRIALEALTRRLGWSIFGV

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family

Homologues:

Organism=Homo sapiens, GI156104878, Length=293, Percent_Identity=37.8839590443686, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI20336214, Length=280, Percent_Identity=36.7857142857143, Blast_Score=184, Evalue=9e-47,
Organism=Escherichia coli, GI1787804, Length=291, Percent_Identity=50.8591065292096, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1786693, Length=287, Percent_Identity=41.8118466898955, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17507019, Length=288, Percent_Identity=37.8472222222222, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI193204073, Length=300, Percent_Identity=37, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17532727, Length=275, Percent_Identity=37.4545454545455, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI193204075, Length=302, Percent_Identity=36.4238410596026, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI281363241, Length=291, Percent_Identity=36.7697594501718, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24653162, Length=291, Percent_Identity=36.7697594501718, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24653166, Length=285, Percent_Identity=37.5438596491228, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI281363239, Length=291, Percent_Identity=36.7697594501718, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653158, Length=285, Percent_Identity=37.5438596491228, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI116008307, Length=285, Percent_Identity=37.5438596491228, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653156, Length=285, Percent_Identity=37.5438596491228, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653164, Length=285, Percent_Identity=37.5438596491228, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLSA_RHOP2 (Q2J097)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485024.1
- ProteinModelPortal:   Q2J097
- SMR:   Q2J097
- STRING:   Q2J097
- GeneID:   3908353
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1403
- eggNOG:   COG2066
- HOGENOM:   HBG512335
- OMA:   NALMLTC
- ProtClustDB:   PRK00971
- BioCyc:   RPAL316058:RPB_1403-MONOMER
- HAMAP:   MF_00313
- InterPro:   IPR012338
- InterPro:   IPR015868
- Gene3D:   G3DSA:3.40.710.20
- PANTHER:   PTHR12544
- TIGRFAMs:   TIGR03814

Pfam domain/function: PF04960 Glutaminase; SSF56601 PBP_transp_fold

EC number: =3.5.1.2

Molecular weight: Translated: 32999; Mature: 32999

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPDLLDHTVAEIAAEMAQRPDRGAVASYIPELAGVDPQRFGLVVIDADGHVAAGGDADM
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCEEEEEECCCCEECCCCCCC
PFSIQSISKVFTLTLALGLAGDRVWRRVGREPSGSAFNSIVQLERERGIPRNPFINAGAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
AVTDLILSGHQPREALGEILRFMQFLAGDDSIVIDDAVAASEQRTGFRNAALANYMKSFG
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHCC
VLDNPVEYTLGVYFHHCAIAMSCRQLALAGRFLAHNGRNPSTGHNVVSTQRARRINALML
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEEE
TCGHYDGSGEFAYRVGLPGKSGVGGGVLAVAPGKASIAVWSPGLDAAGNSHLGRIALEAL
EECCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHH
TRRLGWSIFGV
HHHHCCHHCCC
>Mature Secondary Structure
MNPDLLDHTVAEIAAEMAQRPDRGAVASYIPELAGVDPQRFGLVVIDADGHVAAGGDADM
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCEEEEEECCCCEECCCCCCC
PFSIQSISKVFTLTLALGLAGDRVWRRVGREPSGSAFNSIVQLERERGIPRNPFINAGAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
AVTDLILSGHQPREALGEILRFMQFLAGDDSIVIDDAVAASEQRTGFRNAALANYMKSFG
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHCC
VLDNPVEYTLGVYFHHCAIAMSCRQLALAGRFLAHNGRNPSTGHNVVSTQRARRINALML
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEEE
TCGHYDGSGEFAYRVGLPGKSGVGGGVLAVAPGKASIAVWSPGLDAAGNSHLGRIALEAL
EECCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHH
TRRLGWSIFGV
HHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA