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The map label for this gene is sir1 [H]

Identifier: 86748526

GI number: 86748526

Start: 1592161

End: 1593813

Strand: Direct

Name: sir1 [H]

Synonym: RPB_1401

Alternate gene names: 86748526

Gene position: 1592161-1593813 (Clockwise)

Preceding gene: 86748525

Following gene: 86748527

Centisome position: 29.86

GC content: 64.43

Gene sequence:

>1653_bases
ATGTACGCTTATGACGAGATCGACCAGACGCTGGTCAATGAACGGGTCACCGAATTTCGCGACCAGGTGCGGCGCCGCCT
CAGCGGTGAACTGACCGAGGACGAGTTCAAGCCGTTGCGGCTGATGAACGGCGTCTATCTGCAGCTCCACGCCTATATGT
TCCGCGTCGCGATCCCTTACGGCACGCTGTCGTCGGCGCAGCTGCGCAAGCTCGCACACGTCGCCCGCAAATACGATCGC
GGCTACGGCCATTTCACCACCCGGCAGAACATCCAGTTCAATTGGATCGCGCTGAAGGATCTGCCCGACGCGCTCGCCGA
TCTCGCCGAGGTCGGCATCCACGCGATGCAGACCTCCGGCAACTGCACGCGCAACGTCACTGCCGATCAATGGGCCGGTG
TCGCGCCGGGCGAGGTCGAGGATCCCCGCGTCTGGGCCGAAATCCTGCGCCAGCACACCGCGCTGCATCCGGAATTCTCG
TTTCTGCCGCGCAAGTTCAAATTCGCCATCACCGCAGCCGATCACGACCGCGCGGCCATCAAGGTGCACGATATCGGCCT
GAAGCTGATCAGGAACGAGCAGGGCGAGACCGGTTTCGAGGTGCTGGTCGGCGGCGGGCTCGGCCGTTCGCCGTTCATCG
CCAAGACCATCAAGCCGTTCGTCGCCGGCCGCGACATCCTCAGCTATGTCGAGGCGATCCTGCGCGTTTACAATCAGTAC
GGCCGCCGCGACAACATCTACAAGGCGCGCATCAAGATCCTGGTCCACGAACTCGGCATCGAGAAATTCGCCGCCGAGGT
CGAGCAGACGTGGCGCCAGATCGCCGAAGGTCCGCTGACGCTCGACGACGAGATGATCGAGGACATCCGCGCGCGCTTCG
TCTATCCGGCCTATGAGAGGTTGTCGGACGACCCGGCCGAGTTGCGGGCCGCCGCCGATCCGAAATTCGAGGCGTGGCGC
AGCAATTCGGTCGCGCCGCATCGCCAGCCCGGCTACGCCATCGTCACGCTGTCGCTGAAGCCGGTCGGTGGCCCGCCCGG
CGACGCCACCGCCGATCAGATGGACGCGATTGCCGATCTCGCCGACAGATACTCATTCGGCGAAATCCGCGTCGGCCACG
AGCAGAATCTGGCGCTGCCGCATGTCGCCCAGCGCGATCTGCCGGCGCTGTGGAGCGCGCTCGACGCGCTCGGCGTTGCC
ACGCCGAACGTCAATCTGGTCAGCGACATCATCGCCTGCCCGGGGCTCGACTATTGCTCGCTCGCCAATGCCCGCTCGAT
CCCGATCGCACAGGAGCTGACGCGGCGCTTTTCCAATCACGAGACCGCCAAGCTGATCGGCCGGCTGCACGTCAACATCT
CCGGCTGCATCAACGCCTGCGGCCATCACCATGTCGGCCATATCGGGATTCTCGGCGTCGAGAAGAACGACCAGGAGGTC
TATCAGATCACCATCGGCGGCCGCGCCGACGAGCACGCCAGGCTGGGCGAATTGATCGGGCCCGCGGTGCCCTATGCCGA
GGTCCCCGACGTCATCGAAGACATCGTCGAGGCCTATCTGGCGTTGCGCGACAAGCCGGAGGAGCTGTTCGTCGACACCG
TCAAGCGGCTCGGCGTGCAGCCTTTCAAGGAGCGGGTCTATGCCACTCGTTAA

Upstream 100 bases:

>100_bases
CGACGACACCGGCGCCATCCTTCCCGGCAATCTGCGCGAGCAGATCCGCAACAGCGGTGTCACCATCGAACTGCCGGCTC
ACGCCTGAGGCTTGTGTTTC

Downstream 100 bases:

>100_bases
GCACGGCGCGATCGTCGCCGACGACTACGTCGCGGTCGCCGACGACGCCGACCTTCCCGCGGGCGGTGCGGTGCTGGTTT
CGGCGGCGCGCTTGTTCGCT

Product: sulfite reductase, hemoprotein subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MYAYDEIDQTLVNERVTEFRDQVRRRLSGELTEDEFKPLRLMNGVYLQLHAYMFRVAIPYGTLSSAQLRKLAHVARKYDR
GYGHFTTRQNIQFNWIALKDLPDALADLAEVGIHAMQTSGNCTRNVTADQWAGVAPGEVEDPRVWAEILRQHTALHPEFS
FLPRKFKFAITAADHDRAAIKVHDIGLKLIRNEQGETGFEVLVGGGLGRSPFIAKTIKPFVAGRDILSYVEAILRVYNQY
GRRDNIYKARIKILVHELGIEKFAAEVEQTWRQIAEGPLTLDDEMIEDIRARFVYPAYERLSDDPAELRAAADPKFEAWR
SNSVAPHRQPGYAIVTLSLKPVGGPPGDATADQMDAIADLADRYSFGEIRVGHEQNLALPHVAQRDLPALWSALDALGVA
TPNVNLVSDIIACPGLDYCSLANARSIPIAQELTRRFSNHETAKLIGRLHVNISGCINACGHHHVGHIGILGVEKNDQEV
YQITIGGRADEHARLGELIGPAVPYAEVPDVIEDIVEAYLALRDKPEELFVDTVKRLGVQPFKERVYATR

Sequences:

>Translated_550_residues
MYAYDEIDQTLVNERVTEFRDQVRRRLSGELTEDEFKPLRLMNGVYLQLHAYMFRVAIPYGTLSSAQLRKLAHVARKYDR
GYGHFTTRQNIQFNWIALKDLPDALADLAEVGIHAMQTSGNCTRNVTADQWAGVAPGEVEDPRVWAEILRQHTALHPEFS
FLPRKFKFAITAADHDRAAIKVHDIGLKLIRNEQGETGFEVLVGGGLGRSPFIAKTIKPFVAGRDILSYVEAILRVYNQY
GRRDNIYKARIKILVHELGIEKFAAEVEQTWRQIAEGPLTLDDEMIEDIRARFVYPAYERLSDDPAELRAAADPKFEAWR
SNSVAPHRQPGYAIVTLSLKPVGGPPGDATADQMDAIADLADRYSFGEIRVGHEQNLALPHVAQRDLPALWSALDALGVA
TPNVNLVSDIIACPGLDYCSLANARSIPIAQELTRRFSNHETAKLIGRLHVNISGCINACGHHHVGHIGILGVEKNDQEV
YQITIGGRADEHARLGELIGPAVPYAEVPDVIEDIVEAYLALRDKPEELFVDTVKRLGVQPFKERVYATR
>Mature_550_residues
MYAYDEIDQTLVNERVTEFRDQVRRRLSGELTEDEFKPLRLMNGVYLQLHAYMFRVAIPYGTLSSAQLRKLAHVARKYDR
GYGHFTTRQNIQFNWIALKDLPDALADLAEVGIHAMQTSGNCTRNVTADQWAGVAPGEVEDPRVWAEILRQHTALHPEFS
FLPRKFKFAITAADHDRAAIKVHDIGLKLIRNEQGETGFEVLVGGGLGRSPFIAKTIKPFVAGRDILSYVEAILRVYNQY
GRRDNIYKARIKILVHELGIEKFAAEVEQTWRQIAEGPLTLDDEMIEDIRARFVYPAYERLSDDPAELRAAADPKFEAWR
SNSVAPHRQPGYAIVTLSLKPVGGPPGDATADQMDAIADLADRYSFGEIRVGHEQNLALPHVAQRDLPALWSALDALGVA
TPNVNLVSDIIACPGLDYCSLANARSIPIAQELTRRFSNHETAKLIGRLHVNISGCINACGHHHVGHIGILGVEKNDQEV
YQITIGGRADEHARLGELIGPAVPYAEVPDVIEDIVEAYLALRDKPEELFVDTVKRLGVQPFKERVYATR

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=249, Percent_Identity=26.9076305220884, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322597, Length=559, Percent_Identity=22.1824686940966, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 61517; Mature: 61517

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAYDEIDQTLVNERVTEFRDQVRRRLSGELTEDEFKPLRLMNGVYLQLHAYMFRVAIPY
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTLSSAQLRKLAHVARKYDRGYGHFTTRQNIQFNWIALKDLPDALADLAEVGIHAMQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHEECCC
NCTRNVTADQWAGVAPGEVEDPRVWAEILRQHTALHPEFSFLPRKFKFAITAADHDRAAI
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCEEEEEEEECCCCCCEE
KVHDIGLKLIRNEQGETGFEVLVGGGLGRSPFIAKTIKPFVAGRDILSYVEAILRVYNQY
EEECCCHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRRDNIYKARIKILVHELGIEKFAAEVEQTWRQIAEGPLTLDDEMIEDIRARFVYPAYER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHH
LSDDPAELRAAADPKFEAWRSNSVAPHRQPGYAIVTLSLKPVGGPPGDATADQMDAIADL
CCCCHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHH
ADRYSFGEIRVGHEQNLALPHVAQRDLPALWSALDALGVATPNVNLVSDIIACPGLDYCS
HHHCCCCCEEECCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHH
LANARSIPIAQELTRRFSNHETAKLIGRLHVNISGCINACGHHHVGHIGILGVEKNDQEV
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHEEEHHHHHHHCCCCCCCEEEEEEECCCCCEE
YQITIGGRADEHARLGELIGPAVPYAEVPDVIEDIVEAYLALRDKPEELFVDTVKRLGVQ
EEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
PFKERVYATR
HHHHHHCCCC
>Mature Secondary Structure
MYAYDEIDQTLVNERVTEFRDQVRRRLSGELTEDEFKPLRLMNGVYLQLHAYMFRVAIPY
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTLSSAQLRKLAHVARKYDRGYGHFTTRQNIQFNWIALKDLPDALADLAEVGIHAMQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHEECCC
NCTRNVTADQWAGVAPGEVEDPRVWAEILRQHTALHPEFSFLPRKFKFAITAADHDRAAI
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCEEEEEEEECCCCCCEE
KVHDIGLKLIRNEQGETGFEVLVGGGLGRSPFIAKTIKPFVAGRDILSYVEAILRVYNQY
EEECCCHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRRDNIYKARIKILVHELGIEKFAAEVEQTWRQIAEGPLTLDDEMIEDIRARFVYPAYER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHH
LSDDPAELRAAADPKFEAWRSNSVAPHRQPGYAIVTLSLKPVGGPPGDATADQMDAIADL
CCCCHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHH
ADRYSFGEIRVGHEQNLALPHVAQRDLPALWSALDALGVATPNVNLVSDIIACPGLDYCS
HHHCCCCCEEECCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHH
LANARSIPIAQELTRRFSNHETAKLIGRLHVNISGCINACGHHHVGHIGILGVEKNDQEV
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHEEEHHHHHHHCCCCCCCEEEEEEECCCCCEE
YQITIGGRADEHARLGELIGPAVPYAEVPDVIEDIVEAYLALRDKPEELFVDTVKRLGVQ
EEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
PFKERVYATR
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA