Click here to switch to the map view.

The map label for this gene is cysG [H]

Identifier: 86748524

GI number: 86748524

Start: 1590401

End: 1591837

Strand: Direct

Name: cysG [H]

Synonym: RPB_1399

Alternate gene names: 86748524

Gene position: 1590401-1591837 (Clockwise)

Preceding gene: 86748518

Following gene: 86748525

Centisome position: 29.83

GC content: 69.8

Gene sequence:

>1437_bases
ATGCGTTTTCTTCCGGTCTTTCTCGATCTCGCGGCAGGGCCGGTTGTGCTGGTGGGCGCCGGCGAGATGGCGCGTGCCAA
GCTGCGGCTGCTGCTGTCGGCGGGCGCCGCGATCCGCTGGCATGTCACCGACGGCGACAATGATCTCGCCGGTATCAGCG
AGGCCGACCGCGCGCGCATCACGTTGGCGACGGGGGAACCCGCCGAGGCCGATCTGTCCGGCGCGATCGCGCTGATGTGC
GCCGGCGCCGGCGATCTCGCGGCCCTGCTGGCGGCGAGGGCCCGTGCGCTCGGCGTGCCGGTCAATGTGATGGATGAGCT
GTCGCACTCCACGTTCATCTTTCCGGCGATCGTCGATCGCGGCGATGTGGTGGTCGCGATCGGCACCGGCGGCTCGTCGC
CTGTCGTCGCGCGCCGCGTGCGCGAGATGATCGAGACATTGCTGCCGGCGCGGATCGGCGATCTCGCCGCGCTGATCGGG
CGTTGGCGCAAACCGATCAAGGCGCGGATTGCGGAATTTCCGCTGCGCCGCCGGTTCTGGGAGCGCGTCGTCGACGGCCC
GATCGGCAAGGCGGTGCTCGAAGGGCGCGGCGCCGAGGCCGAGGATCTGCTCGAGGCAATCACCGATCCCTCGGCCTATG
CACGCGATTCTGTTTCAGCGCATACCGAAGGCCACGTCACCCTGGTCGGCGCCGGCCCCGGCGATCCTGATCTCTTGACC
ATCAAGGCGCTGCGCGCGCTGCAGGACGCCGACGTGGTGTTCTACGACGAACTGGTGTCGCCCGAAATTCTCGACCGGAT
CCGCCGCGATGCCGCCCGCGTGCCGGTCGGCCGCCGCATCGGCAAGCCCGGGATCGGCCAGGAGGCGATCAACGCGCTGC
TGATCGAAGCGGCGCAAGCCGGCCAGCGCGCGGTGCGGCTGAAGGGCGGCGATCCGTTCGTGTTCGGCCGCGGCGGTGAA
GAAATCGAGGCGTTGCGCGACGCCGGCGTGTCCTATGCGGTGGTGCCGGGCATCAGCGCGGGCTTCGGTGCGGCGGCGCA
ATTCGAGGTGCCGCTGACCTATCGCCACGAGGCGCTGCGCATCACATTCCTCACCGCGCACAAGGCCAAGGATGCCGAGG
CCGTCGACTGGACGGCGCTCACCGACCAGAAGATGACGGTGGTGGTGTACATGGGCATGGCCGCGGCGCCGACGGTGCGC
GCGGGCCTGCTGACGGCCGGCCGTTCGCCGCAGACGCCGGTCGGCGTGTTCGCCCGGGTAACGCGGCCGGACGCCAAGGC
CGCGGTGGGCACGCTCGACGATCTGCCGGCACTGGTCGAGCAGGTCGAGGTCGGTCCGGCGATCCTGGTGATCGGCGATG
TCGTCGCGCATTCGGCACCGTGGCGCCGGCAACAACTCCAGCAACTCGTGTCGACCTATCAGGTAGCCGCCGAATGA

Upstream 100 bases:

>100_bases
CGCGCGGCGGTGCAAAGGATTGGTTTTCTTTCCGGCAGACTTGTAAGGATTCCAGCTAGAAAATAATTCTGGCGGACCCG
AAAGGGTCAACGGAGCAGCG

Downstream 100 bases:

>100_bases
CCTCTCCACTGCAACAGAAGATCAAGATTACCGGTCCGTCGGTGGTGACGGCGAACCGCACGGTCGACGGCGTGGTGATC
TACCGCACCGCCGATCGCGG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRFLPVFLDLAAGPVVLVGAGEMARAKLRLLLSAGAAIRWHVTDGDNDLAGISEADRARITLATGEPAEADLSGAIALMC
AGAGDLAALLAARARALGVPVNVMDELSHSTFIFPAIVDRGDVVVAIGTGGSSPVVARRVREMIETLLPARIGDLAALIG
RWRKPIKARIAEFPLRRRFWERVVDGPIGKAVLEGRGAEAEDLLEAITDPSAYARDSVSAHTEGHVTLVGAGPGDPDLLT
IKALRALQDADVVFYDELVSPEILDRIRRDAARVPVGRRIGKPGIGQEAINALLIEAAQAGQRAVRLKGGDPFVFGRGGE
EIEALRDAGVSYAVVPGISAGFGAAAQFEVPLTYRHEALRITFLTAHKAKDAEAVDWTALTDQKMTVVVYMGMAAAPTVR
AGLLTAGRSPQTPVGVFARVTRPDAKAAVGTLDDLPALVEQVEVGPAILVIGDVVAHSAPWRRQQLQQLVSTYQVAAE

Sequences:

>Translated_478_residues
MRFLPVFLDLAAGPVVLVGAGEMARAKLRLLLSAGAAIRWHVTDGDNDLAGISEADRARITLATGEPAEADLSGAIALMC
AGAGDLAALLAARARALGVPVNVMDELSHSTFIFPAIVDRGDVVVAIGTGGSSPVVARRVREMIETLLPARIGDLAALIG
RWRKPIKARIAEFPLRRRFWERVVDGPIGKAVLEGRGAEAEDLLEAITDPSAYARDSVSAHTEGHVTLVGAGPGDPDLLT
IKALRALQDADVVFYDELVSPEILDRIRRDAARVPVGRRIGKPGIGQEAINALLIEAAQAGQRAVRLKGGDPFVFGRGGE
EIEALRDAGVSYAVVPGISAGFGAAAQFEVPLTYRHEALRITFLTAHKAKDAEAVDWTALTDQKMTVVVYMGMAAAPTVR
AGLLTAGRSPQTPVGVFARVTRPDAKAAVGTLDDLPALVEQVEVGPAILVIGDVVAHSAPWRRQQLQQLVSTYQVAAE
>Mature_478_residues
MRFLPVFLDLAAGPVVLVGAGEMARAKLRLLLSAGAAIRWHVTDGDNDLAGISEADRARITLATGEPAEADLSGAIALMC
AGAGDLAALLAARARALGVPVNVMDELSHSTFIFPAIVDRGDVVVAIGTGGSSPVVARRVREMIETLLPARIGDLAALIG
RWRKPIKARIAEFPLRRRFWERVVDGPIGKAVLEGRGAEAEDLLEAITDPSAYARDSVSAHTEGHVTLVGAGPGDPDLLT
IKALRALQDADVVFYDELVSPEILDRIRRDAARVPVGRRIGKPGIGQEAINALLIEAAQAGQRAVRLKGGDPFVFGRGGE
EIEALRDAGVSYAVVPGISAGFGAAAQFEVPLTYRHEALRITFLTAHKAKDAEAVDWTALTDQKMTVVVYMGMAAAPTVR
AGLLTAGRSPQTPVGVFARVTRPDAKAAVGTLDDLPALVEQVEVGPAILVIGDVVAHSAPWRRQQLQQLVSTYQVAAE

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=462, Percent_Identity=39.8268398268398, Blast_Score=295, Evalue=5e-81,
Organism=Saccharomyces cerevisiae, GI6322922, Length=233, Percent_Identity=32.1888412017167, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 50444; Mature: 50444

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLPVFLDLAAGPVVLVGAGEMARAKLRLLLSAGAAIRWHVTDGDNDLAGISEADRARI
CCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEE
TLATGEPAEADLSGAIALMCAGAGDLAALLAARARALGVPVNVMDELSHSTFIFPAIVDR
EEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEHEECC
GDVVVAIGTGGSSPVVARRVREMIETLLPARIGDLAALIGRWRKPIKARIAEFPLRRRFW
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHH
ERVVDGPIGKAVLEGRGAEAEDLLEAITDPSAYARDSVSAHTEGHVTLVGAGPGDPDLLT
HHHHCCCCCHHHHCCCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEECCCCCCCEEH
IKALRALQDADVVFYDELVSPEILDRIRRDAARVPVGRRIGKPGIGQEAINALLIEAAQA
HHHHHHHHCCCEEEEHHHCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHC
GQRAVRLKGGDPFVFGRGGEEIEALRDAGVSYAVVPGISAGFGAAAQFEVPLTYRHEALR
CCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCEEECCCEEE
ITFLTAHKAKDAEAVDWTALTDQKMTVVVYMGMAAAPTVRAGLLTAGRSPQTPVGVFARV
EEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCHHHEEEE
TRPDAKAAVGTLDDLPALVEQVEVGPAILVIGDVVAHSAPWRRQQLQQLVSTYQVAAE
CCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRFLPVFLDLAAGPVVLVGAGEMARAKLRLLLSAGAAIRWHVTDGDNDLAGISEADRARI
CCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEE
TLATGEPAEADLSGAIALMCAGAGDLAALLAARARALGVPVNVMDELSHSTFIFPAIVDR
EEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEHEECC
GDVVVAIGTGGSSPVVARRVREMIETLLPARIGDLAALIGRWRKPIKARIAEFPLRRRFW
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHH
ERVVDGPIGKAVLEGRGAEAEDLLEAITDPSAYARDSVSAHTEGHVTLVGAGPGDPDLLT
HHHHCCCCCHHHHCCCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEECCCCCCCEEH
IKALRALQDADVVFYDELVSPEILDRIRRDAARVPVGRRIGKPGIGQEAINALLIEAAQA
HHHHHHHHCCCEEEEHHHCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHC
GQRAVRLKGGDPFVFGRGGEEIEALRDAGVSYAVVPGISAGFGAAAQFEVPLTYRHEALR
CCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCEEECCCEEE
ITFLTAHKAKDAEAVDWTALTDQKMTVVVYMGMAAAPTVRAGLLTAGRSPQTPVGVFARV
EEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCHHHEEEE
TRPDAKAAVGTLDDLPALVEQVEVGPAILVIGDVVAHSAPWRRQQLQQLVSTYQVAAE
CCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA