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The map label for this gene is aadR [H]

Identifier: 86748502

GI number: 86748502

Start: 1569552

End: 1570271

Strand: Direct

Name: aadR [H]

Synonym: RPB_1377

Alternate gene names: 86748502

Gene position: 1569552-1570271 (Clockwise)

Preceding gene: 86748500

Following gene: 86748503

Centisome position: 29.44

GC content: 64.44

Gene sequence:

>720_bases
ATGCCGCACCTCGCTTTTCCGAACTCGACTTGCGACGGGTTTCGTTGCGCGACGCATTGCGCTGTGCGCCCGCTGGCGAT
CTGCGGCGAGCTCGAGCAGGCCGAACACGAGGAGTTCGAGCGCCTCGCGCAGCATGTTCGCTACGGCGCGAAGGAAGCCT
TGTTCTCCGAGGACGAAACCGCCGACGCCGTCTACAGTCTGGTCGAAGGCATCGCCCGGCTCTACAAGCTGCTGCCCGAC
GGCCGACGCCAGATCATCGGATTCGCGCTGCCGGGCGATTTTCTCGGCATGGCGCCGTCCGCGCGGTACTCATTCTCGGC
CGATTCGATCGGCGGCGTGACCGTCTGCAAGTTCTTCCGCGGTCCGTTTCTGCGCTTCATTGAAAACAGGCCGCACATGC
TGATGCGAATGAACGAATTCGCGACCCGGGAGCTCAGCCTTGCGCAGGATCAGATGCTGCTGCTCGGTCGGCGTTCGGCC
GAAGAGAAGGTTGCAGCCTTCCTGGTCGCCTGGCGGGATCGGCTGGCGCGACTGGAAGGCGTCACAAAGACCGTCAGCCT
GCCGATGGGCCGGCAGGACATCGCCGACTTCCTCGGCCTCACGATCGAAACCGTGAGCCGCACCTTCACCAAGCTGGAGC
GCGAAAAGCTCATCGTGATCGTGCCGGACGGGGTTCGCGTCCTCGATCCGAAGCGCTTCGACGCCCTCGCTGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCAACTTGACGATTTGGCGACATATTGGCTTTACGGCCGCATGGCGATCAATCGACGGCAATGCGCGGCAAGAATTTGT
ACGACCGCATGGAGTGATGG

Downstream 100 bases:

>100_bases
CGAGGCTACCTGCAGGCGTGGCCGCCGGGCTATTCAAGGTCCATTGATGCCCGACGTCCGGTCGCTCCACCTCCAAGCCA
GACAGTCTGTCTACGAGATC

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: Anaerobic aromatic degradation regulator [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MPHLAFPNSTCDGFRCATHCAVRPLAICGELEQAEHEEFERLAQHVRYGAKEALFSEDETADAVYSLVEGIARLYKLLPD
GRRQIIGFALPGDFLGMAPSARYSFSADSIGGVTVCKFFRGPFLRFIENRPHMLMRMNEFATRELSLAQDQMLLLGRRSA
EEKVAAFLVAWRDRLARLEGVTKTVSLPMGRQDIADFLGLTIETVSRTFTKLEREKLIVIVPDGVRVLDPKRFDALAAA

Sequences:

>Translated_239_residues
MPHLAFPNSTCDGFRCATHCAVRPLAICGELEQAEHEEFERLAQHVRYGAKEALFSEDETADAVYSLVEGIARLYKLLPD
GRRQIIGFALPGDFLGMAPSARYSFSADSIGGVTVCKFFRGPFLRFIENRPHMLMRMNEFATRELSLAQDQMLLLGRRSA
EEKVAAFLVAWRDRLARLEGVTKTVSLPMGRQDIADFLGLTIETVSRTFTKLEREKLIVIVPDGVRVLDPKRFDALAAA
>Mature_238_residues
PHLAFPNSTCDGFRCATHCAVRPLAICGELEQAEHEEFERLAQHVRYGAKEALFSEDETADAVYSLVEGIARLYKLLPDG
RRQIIGFALPGDFLGMAPSARYSFSADSIGGVTVCKFFRGPFLRFIENRPHMLMRMNEFATRELSLAQDQMLLLGRRSAE
EKVAAFLVAWRDRLARLEGVTKTVSLPMGRQDIADFLGLTIETVSRTFTKLEREKLIVIVPDGVRVLDPKRFDALAAA

Specific function: Transcriptional activator of anaerobic gene expression. For aromatic acid degradation. Also required for the anaerobic degradation of benzoate [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=231, Percent_Identity=26.8398268398268, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26767; Mature: 26636

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHLAFPNSTCDGFRCATHCAVRPLAICGELEQAEHEEFERLAQHVRYGAKEALFSEDET
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCH
ADAVYSLVEGIARLYKLLPDGRRQIIGFALPGDFLGMAPSARYSFSADSIGGVTVCKFFR
HHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHHHH
GPFLRFIENRPHMLMRMNEFATRELSLAQDQMLLLGRRSAEEKVAAFLVAWRDRLARLEG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
VTKTVSLPMGRQDIADFLGLTIETVSRTFTKLEREKLIVIVPDGVRVLDPKRFDALAAA
CHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCHHHHHHHCC
>Mature Secondary Structure 
PHLAFPNSTCDGFRCATHCAVRPLAICGELEQAEHEEFERLAQHVRYGAKEALFSEDET
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCH
ADAVYSLVEGIARLYKLLPDGRRQIIGFALPGDFLGMAPSARYSFSADSIGGVTVCKFFR
HHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHHHH
GPFLRFIENRPHMLMRMNEFATRELSLAQDQMLLLGRRSAEEKVAAFLVAWRDRLARLEG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
VTKTVSLPMGRQDIADFLGLTIETVSRTFTKLEREKLIVIVPDGVRVLDPKRFDALAAA
CHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1522059; 10094687 [H]