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The map label for this gene is fsr [H]

Identifier: 86748433

GI number: 86748433

Start: 1490181

End: 1491443

Strand: Direct

Name: fsr [H]

Synonym: RPB_1308

Alternate gene names: 86748433

Gene position: 1490181-1491443 (Clockwise)

Preceding gene: 86748432

Following gene: 86748434

Centisome position: 27.95

GC content: 66.83

Gene sequence:

>1263_bases
GTGAACAAGCCTGTCGCCGTCGATGTGGATGTGGCCGCGCCGGTCACGACGCCGGATCTGGCGTTGCCCAAGACCACCGC
GCCTGCGCCGCCGCCCGCCGGGCCGGCCTATGTGGTGCTCGGCGGCATCAGCTTTTCGCATTTCCTCAACGACACCATGC
AGTCGCTGATTCCGTCGGTGTATCCGATCCTGAAGGCGAACTACGCGCTCGATTTCGGCCAGATCGGACTGATCACGCTG
GCGTTCCAGTTCACCGCCTCGCTGCTGCAGCCGGTGGTCGGCCACATGACCGACAAGAAGGCGCAGCCGTTCTCGCTGGC
GATCGGCATGGGCTCGACCTTCTTCGGCCTGCTGCTGCTCAGCGTCGCCCATTCCTACACCGTGATCCTGATCGCCGCGG
CGATGATCGGGCTCGGCTCGGCGGTGTTTCACCCGGAATCGGCGCGGATCGCGCGGCTCGCCTCCGGCGGGCGCTACGGT
ATGGCGCAATCGGTGTTCCAGGTCGGCGGCAATGTCGGCACCGCGCTCGGCCCGGTGCTGGCGGCGCTGATCGTGGTGCC
GTTCGGGCAGGGTTCGATCGGCTGGTTCTCGTCGATCGCCTTCGTCGCCATCATCGTGCTGTGGCGCATCGGCCTGTGGT
ACAAGCCGCAGGTCGCCGCCAAGAAGAAGATGGTGGTCAAGCCGCATCCCGACGCGCCCGGCCGGCGGCGGGTGATGGTC
GCGCTCGTCGTGCTGGTGGCGCTGCTGTTCTCCAAGCAGCTCTATATGTCGAGCCTGTCGAGCTACTACACCTTCTATCT
GATCGAGAAGTTCCAGGTCTCGACCCAGACCGCGCAGATGTTCCTGTTCCTGTTCCTCGCCGCCACCGCGGCCGGGGTGT
TCTTCGGCGGCCCGCTCGGCGACCGCTTCGGCCGCCGCTACGTGATCTGGTTCTCGATCCTCGGCACGCTGCCGTTCACG
CTGGCGCTGCCCTATGTCGGGCTCACCGGCAGCGCGGTGCTGACCGTGTTCATCGGTTTCATCCTGGCGTCGGCGACGCC
GGCGATCATCGTGTTCGCGCAGGAACTGATGCCGCACCGCTTCGGCATGATCTCGGGGCTGTTCTTCGGCTTCGCCTTCG
GCATCGGCGGCCTCGGCGCCGCGGCGCTGGGCGAGGTCGCCGATCACCGCGGCATCGATTTCGTCTATCAGGTCTGCTCG
TTCCTGCCGGCGCTGGGCCTGCTCGCCGTGCTGCTGCCGAAGATGCCGCGCCACGCCCATTGA

Upstream 100 bases:

>100_bases
GCGTTCTAGTTTCATCGCGGCCGGCACGATTGCCGGCCGTCTTTCGTTGTCGGCCGCCCGGCTCGTCCCGGGCGCCGCGA
ATATCCCGGGAAACGCCTCC

Downstream 100 bases:

>100_bases
GGCGGCACCGAGGCGCCGGATGACCTCTCCCCGCGCGCGGGGAGAGGTCGCCGCGCATCGCAAGATGCGCGGCGGGTGAG
GGGGCGCTTCCGCGCAGCCG

Product: major facilitator transporter

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNKPVAVDVDVAAPVTTPDLALPKTTAPAPPPAGPAYVVLGGISFSHFLNDTMQSLIPSVYPILKANYALDFGQIGLITL
AFQFTASLLQPVVGHMTDKKAQPFSLAIGMGSTFFGLLLLSVAHSYTVILIAAAMIGLGSAVFHPESARIARLASGGRYG
MAQSVFQVGGNVGTALGPVLAALIVVPFGQGSIGWFSSIAFVAIIVLWRIGLWYKPQVAAKKKMVVKPHPDAPGRRRVMV
ALVVLVALLFSKQLYMSSLSSYYTFYLIEKFQVSTQTAQMFLFLFLAATAAGVFFGGPLGDRFGRRYVIWFSILGTLPFT
LALPYVGLTGSAVLTVFIGFILASATPAIIVFAQELMPHRFGMISGLFFGFAFGIGGLGAAALGEVADHRGIDFVYQVCS
FLPALGLLAVLLPKMPRHAH

Sequences:

>Translated_420_residues
MNKPVAVDVDVAAPVTTPDLALPKTTAPAPPPAGPAYVVLGGISFSHFLNDTMQSLIPSVYPILKANYALDFGQIGLITL
AFQFTASLLQPVVGHMTDKKAQPFSLAIGMGSTFFGLLLLSVAHSYTVILIAAAMIGLGSAVFHPESARIARLASGGRYG
MAQSVFQVGGNVGTALGPVLAALIVVPFGQGSIGWFSSIAFVAIIVLWRIGLWYKPQVAAKKKMVVKPHPDAPGRRRVMV
ALVVLVALLFSKQLYMSSLSSYYTFYLIEKFQVSTQTAQMFLFLFLAATAAGVFFGGPLGDRFGRRYVIWFSILGTLPFT
LALPYVGLTGSAVLTVFIGFILASATPAIIVFAQELMPHRFGMISGLFFGFAFGIGGLGAAALGEVADHRGIDFVYQVCS
FLPALGLLAVLLPKMPRHAH
>Mature_420_residues
MNKPVAVDVDVAAPVTTPDLALPKTTAPAPPPAGPAYVVLGGISFSHFLNDTMQSLIPSVYPILKANYALDFGQIGLITL
AFQFTASLLQPVVGHMTDKKAQPFSLAIGMGSTFFGLLLLSVAHSYTVILIAAAMIGLGSAVFHPESARIARLASGGRYG
MAQSVFQVGGNVGTALGPVLAALIVVPFGQGSIGWFSSIAFVAIIVLWRIGLWYKPQVAAKKKMVVKPHPDAPGRRRVMV
ALVVLVALLFSKQLYMSSLSSYYTFYLIEKFQVSTQTAQMFLFLFLAATAAGVFFGGPLGDRFGRRYVIWFSILGTLPFT
LALPYVGLTGSAVLTVFIGFILASATPAIIVFAQELMPHRFGMISGLFFGFAFGIGGLGAAALGEVADHRGIDFVYQVCS
FLPALGLLAVLLPKMPRHAH

Specific function: Confers the resistance against fosmidomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=396, Percent_Identity=56.0606060606061, Blast_Score=404, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44651; Mature: 44651

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPVAVDVDVAAPVTTPDLALPKTTAPAPPPAGPAYVVLGGISFSHFLNDTMQSLIPSV
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
YPILKANYALDFGQIGLITLAFQFTASLLQPVVGHMTDKKAQPFSLAIGMGSTFFGLLLL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHH
SVAHSYTVILIAAAMIGLGSAVFHPESARIARLASGGRYGMAQSVFQVGGNVGTALGPVL
HHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHH
AALIVVPFGQGSIGWFSSIAFVAIIVLWRIGLWYKPQVAAKKKMVVKPHPDAPGRRRVMV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEECCCCCCCCHHHHHH
ALVVLVALLFSKQLYMSSLSSYYTFYLIEKFQVSTQTAQMFLFLFLAATAAGVFFGGPLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DRFGRRYVIWFSILGTLPFTLALPYVGLTGSAVLTVFIGFILASATPAIIVFAQELMPHR
HHHCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FGMISGLFFGFAFGIGGLGAAALGEVADHRGIDFVYQVCSFLPALGLLAVLLPKMPRHAH
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNKPVAVDVDVAAPVTTPDLALPKTTAPAPPPAGPAYVVLGGISFSHFLNDTMQSLIPSV
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
YPILKANYALDFGQIGLITLAFQFTASLLQPVVGHMTDKKAQPFSLAIGMGSTFFGLLLL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHH
SVAHSYTVILIAAAMIGLGSAVFHPESARIARLASGGRYGMAQSVFQVGGNVGTALGPVL
HHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHH
AALIVVPFGQGSIGWFSSIAFVAIIVLWRIGLWYKPQVAAKKKMVVKPHPDAPGRRRVMV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEECCCCCCCCHHHHHH
ALVVLVALLFSKQLYMSSLSSYYTFYLIEKFQVSTQTAQMFLFLFLAATAAGVFFGGPLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DRFGRRYVIWFSILGTLPFTLALPYVGLTGSAVLTVFIGFILASATPAIIVFAQELMPHR
HHHCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FGMISGLFFGFAFGIGGLGAAALGEVADHRGIDFVYQVCSFLPALGLLAVLLPKMPRHAH
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8917080; 9278503 [H]