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The map label for this gene is aroE

Identifier: 86748427

GI number: 86748427

Start: 1484431

End: 1485273

Strand: Direct

Name: aroE

Synonym: RPB_1302

Alternate gene names: 86748427

Gene position: 1484431-1485273 (Clockwise)

Preceding gene: 86748426

Following gene: 86748431

Centisome position: 27.84

GC content: 70.94

Gene sequence:

>843_bases
ATGCATCCCGGCGACGGTCACACCGCCGCCTGCCTGATCGGCTGGCCCGCGGCGCATTCGCGCTCGCCGCTGATCCATCA
TTACTGGCTGCGGACGCTCGGCATCCCCGGCGGCTATTCGATCGAATCGGTGCCGCCGGAAGGCTTCGCCGAATTCGTCC
TGAACCTGCGGGCGCACGGCTACAGCGGCGCCAACGTCACCATCCCGCACAAGGAGCGCGCGCTGCAACTGACGCAGCCG
GACGACCGCGCCCGCGCGGTCGGCGCCGCCAATACGCTGTATTACGACGGCGATGTGCTGCGCTCGACCAATACCGACGT
CGAGGGCTTCATCGCCAATCTCGACGCCAGCGCGCCGGGCTGGGATCGTACCCCGCATGCCGTGGTGCTCGGCGCCGGCG
GCTCTGCGCGCGCGGTGCTGTTCGGCCTGCTGGAGCGCGGCATCGAACGCATCGCGCTCGCCAACCGCTCGCTCGAGCGC
GCGCAGGCGCTGGCCGATCTGTTCGGCGAACGCGTTGTGCCGATCGCCTGGACCGACGCGCCGGCGGCGCTGCCGGGCGC
CGGCCTGCTGGTCAACACCACCTCGCTCGGCATGAAGGGGCAGCCGTCGCTCGATCTCGATCTCGAGCCGCTGCCGAGCG
ACGCCACCGTCGCCGATCTGGTCTATGTGCCGCTCGAGACCGAACTGCTCGCCGAGGCGCGCGGCCGCGGCCTGCGCACC
GCCGACGGCCTCGGCATGCTGCTGCATCAGGCGGTGCGCGGCTTCGAATTGTGGTTCGGCGCGCGGCCGCACGTCACCGC
CGAACTGCGCGCTTTGGTCGAAGCGGATCTGGCGGCGAAGTAG

Upstream 100 bases:

>100_bases
AACTGATCGGCGAGCGCGCCCGCAGCGAGGACGGCGGCCGCGGCGTGCGGTTGAGGCAGGCGTCGCCGCCGGCAGGGAGG
GACGACGCGTGACGCGGGCG

Downstream 100 bases:

>100_bases
TTCGCCGCCGCAAAGGTCGCGTGGCGCGATCAGTTGTCTCCGCGCCCCGGTCGCGGCCGGTAGCGCGGCAGCGACCAGCC
GCGCTGCAGCGCTGCGGCGC

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MHPGDGHTAACLIGWPAAHSRSPLIHHYWLRTLGIPGGYSIESVPPEGFAEFVLNLRAHGYSGANVTIPHKERALQLTQP
DDRARAVGAANTLYYDGDVLRSTNTDVEGFIANLDASAPGWDRTPHAVVLGAGGSARAVLFGLLERGIERIALANRSLER
AQALADLFGERVVPIAWTDAPAALPGAGLLVNTTSLGMKGQPSLDLDLEPLPSDATVADLVYVPLETELLAEARGRGLRT
ADGLGMLLHQAVRGFELWFGARPHVTAELRALVEADLAAK

Sequences:

>Translated_280_residues
MHPGDGHTAACLIGWPAAHSRSPLIHHYWLRTLGIPGGYSIESVPPEGFAEFVLNLRAHGYSGANVTIPHKERALQLTQP
DDRARAVGAANTLYYDGDVLRSTNTDVEGFIANLDASAPGWDRTPHAVVLGAGGSARAVLFGLLERGIERIALANRSLER
AQALADLFGERVVPIAWTDAPAALPGAGLLVNTTSLGMKGQPSLDLDLEPLPSDATVADLVYVPLETELLAEARGRGLRT
ADGLGMLLHQAVRGFELWFGARPHVTAELRALVEADLAAK
>Mature_280_residues
MHPGDGHTAACLIGWPAAHSRSPLIHHYWLRTLGIPGGYSIESVPPEGFAEFVLNLRAHGYSGANVTIPHKERALQLTQP
DDRARAVGAANTLYYDGDVLRSTNTDVEGFIANLDASAPGWDRTPHAVVLGAGGSARAVLFGLLERGIERIALANRSLER
AQALADLFGERVVPIAWTDAPAALPGAGLLVNTTSLGMKGQPSLDLDLEPLPSDATVADLVYVPLETELLAEARGRGLRT
ADGLGMLLHQAVRGFELWFGARPHVTAELRALVEADLAAK

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=275, Percent_Identity=33.8181818181818, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787983, Length=263, Percent_Identity=34.2205323193916, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320332, Length=272, Percent_Identity=26.4705882352941, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_RHOP2 (Q2J0J8)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484923.1
- HSSP:   Q9SQT8
- ProteinModelPortal:   Q2J0J8
- SMR:   Q2J0J8
- STRING:   Q2J0J8
- GeneID:   3908175
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1302
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   ESVPPEG
- ProtClustDB:   CLSK820486
- BioCyc:   RPAL316058:RPB_1302-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 29776; Mature: 29776

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: ACT_SITE 71-71

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPGDGHTAACLIGWPAAHSRSPLIHHYWLRTLGIPGGYSIESVPPEGFAEFVLNLRAHG
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCC
YSGANVTIPHKERALQLTQPDDRARAVGAANTLYYDGDVLRSTNTDVEGFIANLDASAPG
CCCCEEEECCHHHEEEECCCCHHHHHHCCCCEEEECCCEEECCCCCHHHEEEECCCCCCC
WDRTPHAVVLGAGGSARAVLFGLLERGIERIALANRSLERAQALADLFGERVVPIAWTDA
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
PAALPGAGLLVNTTSLGMKGQPSLDLDLEPLPSDATVADLVYVPLETELLAEARGRGLRT
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCC
ADGLGMLLHQAVRGFELWFGARPHVTAELRALVEADLAAK
HHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHPGDGHTAACLIGWPAAHSRSPLIHHYWLRTLGIPGGYSIESVPPEGFAEFVLNLRAHG
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCC
YSGANVTIPHKERALQLTQPDDRARAVGAANTLYYDGDVLRSTNTDVEGFIANLDASAPG
CCCCEEEECCHHHEEEECCCCHHHHHHCCCCEEEECCCEEECCCCCHHHEEEECCCCCCC
WDRTPHAVVLGAGGSARAVLFGLLERGIERIALANRSLERAQALADLFGERVVPIAWTDA
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
PAALPGAGLLVNTTSLGMKGQPSLDLDLEPLPSDATVADLVYVPLETELLAEARGRGLRT
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCC
ADGLGMLLHQAVRGFELWFGARPHVTAELRALVEADLAAK
HHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA