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The map label for this gene is fliF [H]

Identifier: 86748397

GI number: 86748397

Start: 1458328

End: 1459926

Strand: Direct

Name: fliF [H]

Synonym: RPB_1272

Alternate gene names: 86748397

Gene position: 1458328-1459926 (Clockwise)

Preceding gene: 86748396

Following gene: 86748398

Centisome position: 27.35

GC content: 67.1

Gene sequence:

>1599_bases
TTGCAGAGTTTGCTGGGTTTCCTGAAAGGCTTGGGCGCCGCCCGGGTGATGGCCATGATCGCCGTCACCATGGCGCTGCT
CGGCTTTTTCGCCTTCGTCATCATGCGCGTGTCGCAGCCGCAGATGACGACCCTCTATACCGACCTCAGCGTCGAGGACT
CCTCCAGCATCATCAAGGAGCTGGAGCGCCAGGCGATCCCGTTCGAAATGCGCAATGACGGCGCCATTCTGATGGTGCCG
AAAGACAAGGTGACCCGGCTGCGGATGAAGCTGGCCGAGGGCGGACTGCCGAAGGGCGGCGGCGTCGGCTACGAGATCTT
CGACAAATCCGACGCGCTCGGCACCACCAGCTTCGTCCAGAACATCAACCATCTCCGCGCGCTGGAAGGCGAATTGTCCC
GCACCATCCGGGCGATCGACCGGGTTCAGGCGGCGCGGGTGCATCTGGTGCTGCCGGAGCGGCCGTTGTTCTCGCGCGAG
ACGCCGGAGCCGTCGGCGTCGATCGTGCTGAGGGTGCGCGGCGCGCTGGAGCCGCAGCAGGTCCGCGCCATCCGCCATCT
GGTCGCCTCCGCGGTCAACGGCCTGAAGCCGCAGCGGGTCTCGATCGTCGACGAGGGCGGCCAGTTGCTCGCCGACGGCT
CGGCCAGCGCCAATGCCGCCGACGGCGCCGCCGGCGACGAACGCCGCACCAGCTTCGAGAAGCGGATGCGCAACGAGATC
GAGGGCATCGTGTCCTCGGTCGTCGGCTCCGGCCGCGCCCGGGTCCAGGTCACCGCCGATTTCGATTACAACAAGATCAC
CCAGACTTCGGACAAGTTCGACCCCGAAGGCCGGGTGCTGCGCTCCAGCCAGACCCGCGAAGAGCAGGCGCAGACCTCCG
CGGCCGACGGCCAGGTCACCGTCAACAACGAACTGCCGGGCAATCAGGCCCCGGGCGGCGCCGGCGCGCGGGACCAGAGC
AAGAAGTCCGAGGAGACCAACAATTACGAAATCTCCCGCGTCACCAAGACCGAGGTGACCGAGGCCGGCCGGGTCAACCG
GCTGTCGGTCGCGGTGCTGGTCGACGGCAGTTACACCAAAGACGAGAAGGGCGAGCTGGTCTACGCCGAGCGGCCGAAGG
AGCAGCTCGACCGCATCGCCGCGCTGGTGCGCTCGGCGATCGGCTTCGACCAGAAGCGCGGCGACCAGATCGAGGTCGTC
AATCTGAGATTCGCGGAGGCGCCGCAGGTCGAGAAACTGCCGGAGCCCGCCGGCCTGCTCGGCATGCTGCAATTCACCAA
GGACGACATCATGAACATGATTCAGCTCGGCGTGATGTTCGTGCTCGGCCTGGTGGTGCTGTTCATGGTGATCCGCCCGC
TGGTCAAGCGCGTGCTCGCCTCCGACCCGGCGTCCGAAGCCAGCGGCCTGCCGGCGCTGGCCGACGGCAGCCTGCCGCAA
ATCGGCTCCGACGGGCAGGCCGGCACCAGCATGATCGACATCGCCCAGGTGCAGGGCCAGGTTCACGCCCAGTCGGTCCA
CCGCGTCGGCGAACTCGCCGAGCGCAATCCCAACGAAACCGCAGCCATTGTCCGTCAATGGCTGGCCGAGCCGGCATAA

Upstream 100 bases:

>100_bases
CAATTCCGCGTCCGGTTAAGATTCGTTAACCTTATGGAAACCATCCCATAGGCAAGATTTGCCTAGTCGGCTTTGTCGTG
CCGAATCCGAGGGGCTGGTC

Downstream 100 bases:

>100_bases
ACCGACATGGCCGAGACGCCCGCAACAACACCCGGCACCGAAGACATCGCCAGCGTCGTCGCGACGCTCGCGCAGCGTCA
GAACGGCCGCCCCGCCTCGA

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MQSLLGFLKGLGAARVMAMIAVTMALLGFFAFVIMRVSQPQMTTLYTDLSVEDSSSIIKELERQAIPFEMRNDGAILMVP
KDKVTRLRMKLAEGGLPKGGGVGYEIFDKSDALGTTSFVQNINHLRALEGELSRTIRAIDRVQAARVHLVLPERPLFSRE
TPEPSASIVLRVRGALEPQQVRAIRHLVASAVNGLKPQRVSIVDEGGQLLADGSASANAADGAAGDERRTSFEKRMRNEI
EGIVSSVVGSGRARVQVTADFDYNKITQTSDKFDPEGRVLRSSQTREEQAQTSAADGQVTVNNELPGNQAPGGAGARDQS
KKSEETNNYEISRVTKTEVTEAGRVNRLSVAVLVDGSYTKDEKGELVYAERPKEQLDRIAALVRSAIGFDQKRGDQIEVV
NLRFAEAPQVEKLPEPAGLLGMLQFTKDDIMNMIQLGVMFVLGLVVLFMVIRPLVKRVLASDPASEASGLPALADGSLPQ
IGSDGQAGTSMIDIAQVQGQVHAQSVHRVGELAERNPNETAAIVRQWLAEPA

Sequences:

>Translated_532_residues
MQSLLGFLKGLGAARVMAMIAVTMALLGFFAFVIMRVSQPQMTTLYTDLSVEDSSSIIKELERQAIPFEMRNDGAILMVP
KDKVTRLRMKLAEGGLPKGGGVGYEIFDKSDALGTTSFVQNINHLRALEGELSRTIRAIDRVQAARVHLVLPERPLFSRE
TPEPSASIVLRVRGALEPQQVRAIRHLVASAVNGLKPQRVSIVDEGGQLLADGSASANAADGAAGDERRTSFEKRMRNEI
EGIVSSVVGSGRARVQVTADFDYNKITQTSDKFDPEGRVLRSSQTREEQAQTSAADGQVTVNNELPGNQAPGGAGARDQS
KKSEETNNYEISRVTKTEVTEAGRVNRLSVAVLVDGSYTKDEKGELVYAERPKEQLDRIAALVRSAIGFDQKRGDQIEVV
NLRFAEAPQVEKLPEPAGLLGMLQFTKDDIMNMIQLGVMFVLGLVVLFMVIRPLVKRVLASDPASEASGLPALADGSLPQ
IGSDGQAGTSMIDIAQVQGQVHAQSVHRVGELAERNPNETAAIVRQWLAEPA
>Mature_532_residues
MQSLLGFLKGLGAARVMAMIAVTMALLGFFAFVIMRVSQPQMTTLYTDLSVEDSSSIIKELERQAIPFEMRNDGAILMVP
KDKVTRLRMKLAEGGLPKGGGVGYEIFDKSDALGTTSFVQNINHLRALEGELSRTIRAIDRVQAARVHLVLPERPLFSRE
TPEPSASIVLRVRGALEPQQVRAIRHLVASAVNGLKPQRVSIVDEGGQLLADGSASANAADGAAGDERRTSFEKRMRNEI
EGIVSSVVGSGRARVQVTADFDYNKITQTSDKFDPEGRVLRSSQTREEQAQTSAADGQVTVNNELPGNQAPGGAGARDQS
KKSEETNNYEISRVTKTEVTEAGRVNRLSVAVLVDGSYTKDEKGELVYAERPKEQLDRIAALVRSAIGFDQKRGDQIEVV
NLRFAEAPQVEKLPEPAGLLGMLQFTKDDIMNMIQLGVMFVLGLVVLFMVIRPLVKRVLASDPASEASGLPALADGSLPQ
IGSDGQAGTSMIDIAQVQGQVHAQSVHRVGELAERNPNETAAIVRQWLAEPA

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=425, Percent_Identity=36.2352941176471, Blast_Score=238, Evalue=6e-64,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 57575; Mature: 57575

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLLGFLKGLGAARVMAMIAVTMALLGFFAFVIMRVSQPQMTTLYTDLSVEDSSSIIKE
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
LERQAIPFEMRNDGAILMVPKDKVTRLRMKLAEGGLPKGGGVGYEIFDKSDALGTTSFVQ
HHHHCCCEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHH
NINHLRALEGELSRTIRAIDRVQAARVHLVLPERPLFSRETPEPSASIVLRVRGALEPQQ
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHH
VRAIRHLVASAVNGLKPQRVSIVDEGGQLLADGSASANAADGAAGDERRTSFEKRMRNEI
HHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EGIVSSVVGSGRARVQVTADFDYNKITQTSDKFDPEGRVLRSSQTREEQAQTSAADGQVT
HHHHHHHHCCCCEEEEEEECCCCCHHCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCEEE
VNNELPGNQAPGGAGARDQSKKSEETNNYEISRVTKTEVTEAGRVNRLSVAVLVDGSYTK
ECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCEEEEEEEECCCCCC
DEKGELVYAERPKEQLDRIAALVRSAIGFDQKRGDQIEVVNLRFAEAPQVEKLPEPAGLL
CCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHCCCCCHHHH
GMLQFTKDDIMNMIQLGVMFVLGLVVLFMVIRPLVKRVLASDPASEASGLPALADGSLPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCC
IGSDGQAGTSMIDIAQVQGQVHAQSVHRVGELAERNPNETAAIVRQWLAEPA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQSLLGFLKGLGAARVMAMIAVTMALLGFFAFVIMRVSQPQMTTLYTDLSVEDSSSIIKE
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
LERQAIPFEMRNDGAILMVPKDKVTRLRMKLAEGGLPKGGGVGYEIFDKSDALGTTSFVQ
HHHHCCCEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHH
NINHLRALEGELSRTIRAIDRVQAARVHLVLPERPLFSRETPEPSASIVLRVRGALEPQQ
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHH
VRAIRHLVASAVNGLKPQRVSIVDEGGQLLADGSASANAADGAAGDERRTSFEKRMRNEI
HHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EGIVSSVVGSGRARVQVTADFDYNKITQTSDKFDPEGRVLRSSQTREEQAQTSAADGQVT
HHHHHHHHCCCCEEEEEEECCCCCHHCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCEEE
VNNELPGNQAPGGAGARDQSKKSEETNNYEISRVTKTEVTEAGRVNRLSVAVLVDGSYTK
ECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCEEEEEEEECCCCCC
DEKGELVYAERPKEQLDRIAALVRSAIGFDQKRGDQIEVVNLRFAEAPQVEKLPEPAGLL
CCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHCCCCCHHHH
GMLQFTKDDIMNMIQLGVMFVLGLVVLFMVIRPLVKRVLASDPASEASGLPALADGSLPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCC
IGSDGQAGTSMIDIAQVQGQVHAQSVHRVGELAERNPNETAAIVRQWLAEPA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002583; 11259647 [H]