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The map label for this gene is cobS [H]

Identifier: 86748384

GI number: 86748384

Start: 1440483

End: 1441283

Strand: Reverse

Name: cobS [H]

Synonym: RPB_1259

Alternate gene names: 86748384

Gene position: 1441283-1440483 (Counterclockwise)

Preceding gene: 86748402

Following gene: 86748376

Centisome position: 27.03

GC content: 70.54

Gene sequence:

>801_bases
ATGTCGATCGATTCCCGGCCCCCGCGGCCTTCGCCGACGCTCGCGCAACTGCTCGATGCGATTCGCTTCCTGACCATCCT
GCCGGTGCCGGATTCCGACCGCGCGCCGGAGCCGGACTGGCTGACCCGGGCGTTGGCGTTCTTTCCGATCGTCGGCGTCG
GCATCGGTGTGATCGCAGCGACCGTGCTGCTGATCGGCAATTCGCTGTTCGGCCCCGGGCTCGCCGCGCTGCTGGCGGTC
GCCGCCTCGGTCGTCGTCACCGGGGCGATGCACGAGGACGGCCTCGCCGACACGTTCGACAGTTTCGGCGGCGGCTGGTC
GGTGGCCAAGCGGCTGGAGATCATGAAGGACTCGCGGCTCGGCACCTATGGCGGCCTGGCGCTCGGCCTCGATGTGGCGC
TGCGCGCCATGGCGCTGGCGCAATTGCCGCTGTGGGCCGCGCTCGCCACGCTGGTCGCCTGCCATGCCGCCGCGCGCGCG
ACGCCCGGTTTCGTCAAGCGCAAGCTGTCCTACGCCGGCGACGTCAGCGGCATGAAGGTGAGCTACGCCGAAGAGCCGAT
GCGCAAGGATGAATGGGCGCTGGTGTTGATCACCGTCGCGCTGGCGGCGCTGCCGCTGCTGCTGGTGTCGCCGCTGGCGC
TGATCGCCGGCCTCGGCTTCGGCGCGGCGCTGGCCGCCCTGATAGCCTCATGGGCGAAGCGTCTGCTCGGCGGCTACACC
GGCGACGTGCTCGGCGCCACCGAGCAGATGTTCGAGATCGGGTATCTGCTGGGTGTGGCCGCGGTGCTGGCGGGGAGTTA
G

Upstream 100 bases:

>100_bases
GGCTGCGGATCGCGCAATCACGCCGGCCGGCCATGGCCAAGGTCGCAGTTGACCGCGGCGTGCCGAGGCGCGCAGTAGAG
CCTCCCCGCAGAGGACCCGC

Downstream 100 bases:

>100_bases
TCCGACAACCTCCCGTCATTGCGAGGAGCGAAGCGACGAAGCAATCCAGCGCCGCATGCGGGGCGTGGATTGCTTCGCTT
CGCTCGCAATGACGGAGAGG

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSIDSRPPRPSPTLAQLLDAIRFLTILPVPDSDRAPEPDWLTRALAFFPIVGVGIGVIAATVLLIGNSLFGPGLAALLAV
AASVVVTGAMHEDGLADTFDSFGGGWSVAKRLEIMKDSRLGTYGGLALGLDVALRAMALAQLPLWAALATLVACHAAARA
TPGFVKRKLSYAGDVSGMKVSYAEEPMRKDEWALVLITVALAALPLLLVSPLALIAGLGFGAALAALIASWAKRLLGGYT
GDVLGATEQMFEIGYLLGVAAVLAGS

Sequences:

>Translated_266_residues
MSIDSRPPRPSPTLAQLLDAIRFLTILPVPDSDRAPEPDWLTRALAFFPIVGVGIGVIAATVLLIGNSLFGPGLAALLAV
AASVVVTGAMHEDGLADTFDSFGGGWSVAKRLEIMKDSRLGTYGGLALGLDVALRAMALAQLPLWAALATLVACHAAARA
TPGFVKRKLSYAGDVSGMKVSYAEEPMRKDEWALVLITVALAALPLLLVSPLALIAGLGFGAALAALIASWAKRLLGGYT
GDVLGATEQMFEIGYLLGVAAVLAGS
>Mature_265_residues
SIDSRPPRPSPTLAQLLDAIRFLTILPVPDSDRAPEPDWLTRALAFFPIVGVGIGVIAATVLLIGNSLFGPGLAALLAVA
ASVVVTGAMHEDGLADTFDSFGGGWSVAKRLEIMKDSRLGTYGGLALGLDVALRAMALAQLPLWAALATLVACHAAARAT
PGFVKRKLSYAGDVSGMKVSYAEEPMRKDEWALVLITVALAALPLLLVSPLALIAGLGFGAALAALIASWAKRLLGGYTG
DVLGATEQMFEIGYLLGVAAVLAGS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=34.1463414634146, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27447; Mature: 27315

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDSRPPRPSPTLAQLLDAIRFLTILPVPDSDRAPEPDWLTRALAFFPIVGVGIGVIAA
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TVLLIGNSLFGPGLAALLAVAASVVVTGAMHEDGLADTFDSFGGGWSVAKRLEIMKDSRL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCC
GTYGGLALGLDVALRAMALAQLPLWAALATLVACHAAARATPGFVKRKLSYAGDVSGMKV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
SYAEEPMRKDEWALVLITVALAALPLLLVSPLALIAGLGFGAALAALIASWAKRLLGGYT
EHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
GDVLGATEQMFEIGYLLGVAAVLAGS
CHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIDSRPPRPSPTLAQLLDAIRFLTILPVPDSDRAPEPDWLTRALAFFPIVGVGIGVIAA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TVLLIGNSLFGPGLAALLAVAASVVVTGAMHEDGLADTFDSFGGGWSVAKRLEIMKDSRL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCC
GTYGGLALGLDVALRAMALAQLPLWAALATLVACHAAARATPGFVKRKLSYAGDVSGMKV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
SYAEEPMRKDEWALVLITVALAALPLLLVSPLALIAGLGFGAALAALIASWAKRLLGGYT
EHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
GDVLGATEQMFEIGYLLGVAAVLAGS
CHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA