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The map label for this gene is oprF [H]

Identifier: 86747898

GI number: 86747898

Start: 865318

End: 866712

Strand: Direct

Name: oprF [H]

Synonym: RPB_0772

Alternate gene names: 86747898

Gene position: 865318-866712 (Clockwise)

Preceding gene: 86747895

Following gene: 86747899

Centisome position: 16.23

GC content: 69.32

Gene sequence:

>1395_bases
ATGCGCGGACTTTTCAGATCGAGCGGCCAGTGGTGGCGCGGCGCCATCATCCTGATCGCTCTATGGTCGATCGCGGCCTG
GACCACCACGCCGGACGTAGAAGCCGATGTCGCAGCGCGTTCCGTCGCGGCGCTCAAGAGCACCGTGCTGGACAAAGCCC
GGCTGACGCCGGTCGGTCGCGACATCGTCTTCTCCGCCGATGCGTTTTCGGAAGAAGGCCGCCGCAGTGCGGTCGCCGCG
GTCGAGGCCGTCCCCGGCGTCCGGCTGGTGGTCGATCAGACCCGCCTGGTGCCGCAGGCGACGCCGTTCGTCTGGTCGAT
CGAGCGCGACGTCGTTCGCGTCACGCTCGCCGGCAGCGCGCCGTTGCCCGCCAGCCGGGCGCGGCTCGCGGATGCCGCCC
GCGCCGAACTGGCCGGCGTCGAGGTCGCCGACCGCATGACGCTCGCCCGCGGCGCGCCGACCCGCTTCGATGCCGCCGCT
CTGCTGCTGGTCGATCAGATCGGCAAGCTCAAGGAAGGCAAGATCACCCTGTCCGACACCAAGGTCAGTCTCGCCGGCAT
GGCGCGTGATCTCGGCGGTCGCGAGGCCATCGGCGCAGCGCTGCGGAACCTGCCGGAGGGCTATGCGGTCGCCGCGAACG
ACATCAAGGCGCCGCCTTACGTCTTCCAGGCCAACAAGGACCCGGTGGCGTCGACCCTGACCCTGACCGGCTACGTGCCG
GATAATAATGTCCATGCGGCGCTGGCGGCGGCCGCCGGCCGGAAATTCTTCAGCGAGAAGGTGGTCGACCAGCTCAAGGC
CAGCATCGGGGCGCCGCAGGGCTTCGCCGGCGCGGTGGCTCCGGCGCTCGGCGCGCTGTCGCGGCTCTCGACCGGCACGC
TGGTGGTGTCCGACCGCGAGGTGCGGCTGTCCGGCGACGCGCTGTTCGAGGCGGCGGTCAGCCAGATCCGCAGCGAGCTG
AACAAGGACATGCCGCAGGGCTGGACCGTCAAGGCCGAGCTCTCGGTCAAGCCGGCGGCGTCGCCGGTCGATGCCTCGGT
CTGCCAGCAATTGTTCGACGATCTGCTCGGCAAAAGCACGGTGCGCTTCGAATCCGCCAGCGCCACCATCGACAAGGATT
CCGCCGGTCTGCTCGATCGGCTGATCGAGACCGCGATGCGCTGTCCCGGCGCCTCGATCGAGGTCGCCGGCCACACCGAC
AGCGACGGCGAGGCCGGTTTCAACCAGGCGCTGTCGGAGAAGCGCGCGCGCGCGGTGGTCGACTATATGGTCAGGGCCGG
TCTCACGCCCGAGCGCTTCCAGGCGTTCGGCTACGGCAGCAGGATTCCGCTCGCCGGCAACGACAGCGACGCTGGCAAGG
CGAAAAATCGCCGCATCGATTTCGTGATCAGGTGA

Upstream 100 bases:

>100_bases
GTTATAGACGACTGCCGGATTGGCGGCTTTTTTCTTGAATTGCGTTTGACAGCCTTCCAACGGTTTGATTTCTCTCTGCT
TCTGACCCACGGGAAGCGGA

Downstream 100 bases:

>100_bases
CGGCATGATGGCGATGGCGAATTTCTACGGGGGCTGGTTGCTCGGCGCGCTGCTGCTGGGGTTCGCCGCAGGCTGGATCG
CGGTGGTGCAACGCGGCGAG

Product: OmpA/MotB

Products: NA

Alternate protein names: Root adhesin [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRGLFRSSGQWWRGAIILIALWSIAAWTTTPDVEADVAARSVAALKSTVLDKARLTPVGRDIVFSADAFSEEGRRSAVAA
VEAVPGVRLVVDQTRLVPQATPFVWSIERDVVRVTLAGSAPLPASRARLADAARAELAGVEVADRMTLARGAPTRFDAAA
LLLVDQIGKLKEGKITLSDTKVSLAGMARDLGGREAIGAALRNLPEGYAVAANDIKAPPYVFQANKDPVASTLTLTGYVP
DNNVHAALAAAAGRKFFSEKVVDQLKASIGAPQGFAGAVAPALGALSRLSTGTLVVSDREVRLSGDALFEAAVSQIRSEL
NKDMPQGWTVKAELSVKPAASPVDASVCQQLFDDLLGKSTVRFESASATIDKDSAGLLDRLIETAMRCPGASIEVAGHTD
SDGEAGFNQALSEKRARAVVDYMVRAGLTPERFQAFGYGSRIPLAGNDSDAGKAKNRRIDFVIR

Sequences:

>Translated_464_residues
MRGLFRSSGQWWRGAIILIALWSIAAWTTTPDVEADVAARSVAALKSTVLDKARLTPVGRDIVFSADAFSEEGRRSAVAA
VEAVPGVRLVVDQTRLVPQATPFVWSIERDVVRVTLAGSAPLPASRARLADAARAELAGVEVADRMTLARGAPTRFDAAA
LLLVDQIGKLKEGKITLSDTKVSLAGMARDLGGREAIGAALRNLPEGYAVAANDIKAPPYVFQANKDPVASTLTLTGYVP
DNNVHAALAAAAGRKFFSEKVVDQLKASIGAPQGFAGAVAPALGALSRLSTGTLVVSDREVRLSGDALFEAAVSQIRSEL
NKDMPQGWTVKAELSVKPAASPVDASVCQQLFDDLLGKSTVRFESASATIDKDSAGLLDRLIETAMRCPGASIEVAGHTD
SDGEAGFNQALSEKRARAVVDYMVRAGLTPERFQAFGYGSRIPLAGNDSDAGKAKNRRIDFVIR
>Mature_464_residues
MRGLFRSSGQWWRGAIILIALWSIAAWTTTPDVEADVAARSVAALKSTVLDKARLTPVGRDIVFSADAFSEEGRRSAVAA
VEAVPGVRLVVDQTRLVPQATPFVWSIERDVVRVTLAGSAPLPASRARLADAARAELAGVEVADRMTLARGAPTRFDAAA
LLLVDQIGKLKEGKITLSDTKVSLAGMARDLGGREAIGAALRNLPEGYAVAANDIKAPPYVFQANKDPVASTLTLTGYVP
DNNVHAALAAAAGRKFFSEKVVDQLKASIGAPQGFAGAVAPALGALSRLSTGTLVVSDREVRLSGDALFEAAVSQIRSEL
NKDMPQGWTVKAELSVKPAASPVDASVCQQLFDDLLGKSTVRFESASATIDKDSAGLLDRLIETAMRCPGASIEVAGHTD
SDGEAGFNQALSEKRARAVVDYMVRAGLTPERFQAFGYGSRIPLAGNDSDAGKAKNRRIDFVIR

Specific function: Known to stabilize the outer membrane [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=105, Percent_Identity=32.3809523809524, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1787191, Length=105, Percent_Identity=35.2380952380952, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 48964; Mature: 48964

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLFRSSGQWWRGAIILIALWSIAAWTTTPDVEADVAARSVAALKSTVLDKARLTPVGR
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DIVFSADAFSEEGRRSAVAAVEAVPGVRLVVDQTRLVPQATPFVWSIERDVVRVTLAGSA
CEEEECHHHHHCCHHHHHHHHHHCCCEEEEEECHHCCCCCCCEEEEECCCEEEEEEECCC
PLPASRARLADAARAELAGVEVADRMTLARGAPTRFDAAALLLVDQIGKLKEGKITLSDT
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECC
KVSLAGMARDLGGREAIGAALRNLPEGYAVAANDIKAPPYVFQANKDPVASTLTLTGYVP
EEHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCEEEECCCCCCCEEEEEEEECC
DNNVHAALAAAAGRKFFSEKVVDQLKASIGAPQGFAGAVAPALGALSRLSTGTLVVSDRE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCE
VRLSGDALFEAAVSQIRSELNKDMPQGWTVKAELSVKPAASPVDASVCQQLFDDLLGKST
EEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCE
VRFESASATIDKDSAGLLDRLIETAMRCPGASIEVAGHTDSDGEAGFNQALSEKRARAVV
EEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
DYMVRAGLTPERFQAFGYGSRIPLAGNDSDAGKAKNRRIDFVIR
HHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MRGLFRSSGQWWRGAIILIALWSIAAWTTTPDVEADVAARSVAALKSTVLDKARLTPVGR
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DIVFSADAFSEEGRRSAVAAVEAVPGVRLVVDQTRLVPQATPFVWSIERDVVRVTLAGSA
CEEEECHHHHHCCHHHHHHHHHHCCCEEEEEECHHCCCCCCCEEEEECCCEEEEEEECCC
PLPASRARLADAARAELAGVEVADRMTLARGAPTRFDAAALLLVDQIGKLKEGKITLSDT
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECC
KVSLAGMARDLGGREAIGAALRNLPEGYAVAANDIKAPPYVFQANKDPVASTLTLTGYVP
EEHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCEEEECCCCCCCEEEEEEEECC
DNNVHAALAAAAGRKFFSEKVVDQLKASIGAPQGFAGAVAPALGALSRLSTGTLVVSDRE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCE
VRLSGDALFEAAVSQIRSELNKDMPQGWTVKAELSVKPAASPVDASVCQQLFDDLLGKST
EEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCE
VRFESASATIDKDSAGLLDRLIETAMRCPGASIEVAGHTDSDGEAGFNQALSEKRARAVV
EEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
DYMVRAGLTPERFQAFGYGSRIPLAGNDSDAGKAKNRRIDFVIR
HHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1538702 [H]