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The map label for this gene is hisF [H]

Identifier: 86747535

GI number: 86747535

Start: 452773

End: 453540

Strand: Direct

Name: hisF [H]

Synonym: RPB_0409

Alternate gene names: 86747535

Gene position: 452773-453540 (Clockwise)

Preceding gene: 86747534

Following gene: 86747536

Centisome position: 8.49

GC content: 65.49

Gene sequence:

>768_bases
ATGTTCAAGGTCCGCGTCATTCCCTGCCTCGACGTCAAGGACGGCCGCGTCGTCAAGGGCGTCAACTTCGTCGATCTGCG
CGACGCCGGTGATCCGGTGGAAGCTGCGATCGCTTATGACGCCGCCGGCGCCGACGAATTGTGCTTCCTCGATATCACCG
CGACCCATGAGAACCGCGGCATCATGCTCGACGTGGTGCGACGCACGGCGGAAGCCTGCTTCATGCCGGTGACGGTCGGC
GGCGGTGTCCGCACCGTCGACGACATCAAGACGCTGCTGCGCTCCGGCGCCGACAAGGTCTCGATCAATTCCGCCGCGGT
GGCGCGCCGCGAGTTCGTCAAGGAAGCGGCGGAGAAATTCGGCGACCAGTGCATCGTGGTGGCGATCGACGCCAAGCGCG
TACCGGGCCGCGATCGTTGGGAGATCTTCACCCATGGCGGCCGCAAGGGCACCGGCATCGACGCCATCGAATTCGCGCAG
GAAGTAGTGTCGCTCGGCGCCGGCGAGATCCTGCTGACCTCGATGGATCGCGACGGTACGCGATCGGGGTTCGATCTGCC
GCTGACCCGCGCGATCGCCGACAGCATCCAGGTCCCGGTGATCGCCTCGGGCGGTGTCGGCAATCTCGATCATCTGGTCG
ACGGCATCCGCGACGGCCACGCCACGGCGGTTCTGGCGGCGTCGATCTTCCATTTCGGCGAGTACACCATTCGTCAGGCC
AAGGATCATATGGTTCGCTGCGGGCTGCCGATGCGGCTCGATCCCTAG

Upstream 100 bases:

>100_bases
GGAAGGCGCGATCGCCGGCCGTGCGCTGTATGACGGCCGGCTCGACCCGGCGGAAGCGCTGGCGCTGATCGGCGCCGCCA
GAGCGGCTTGAGGACGAGCG

Downstream 100 bases:

>100_bases
ATTCGGGCCGGCGGAGGCGGTAGTCTCGCACCGGCAAAACCGGCAGAGTAGGCGTATGGCCCGTTTCACCCTTCACGATC
TCGAAGCCACCATCGAGGCC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MFKVRVIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAIAYDAAGADELCFLDITATHENRGIMLDVVRRTAEACFMPVTVG
GGVRTVDDIKTLLRSGADKVSINSAAVARREFVKEAAEKFGDQCIVVAIDAKRVPGRDRWEIFTHGGRKGTGIDAIEFAQ
EVVSLGAGEILLTSMDRDGTRSGFDLPLTRAIADSIQVPVIASGGVGNLDHLVDGIRDGHATAVLAASIFHFGEYTIRQA
KDHMVRCGLPMRLDP

Sequences:

>Translated_255_residues
MFKVRVIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAIAYDAAGADELCFLDITATHENRGIMLDVVRRTAEACFMPVTVG
GGVRTVDDIKTLLRSGADKVSINSAAVARREFVKEAAEKFGDQCIVVAIDAKRVPGRDRWEIFTHGGRKGTGIDAIEFAQ
EVVSLGAGEILLTSMDRDGTRSGFDLPLTRAIADSIQVPVIASGGVGNLDHLVDGIRDGHATAVLAASIFHFGEYTIRQA
KDHMVRCGLPMRLDP
>Mature_255_residues
MFKVRVIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAIAYDAAGADELCFLDITATHENRGIMLDVVRRTAEACFMPVTVG
GGVRTVDDIKTLLRSGADKVSINSAAVARREFVKEAAEKFGDQCIVVAIDAKRVPGRDRWEIFTHGGRKGTGIDAIEFAQ
EVVSLGAGEILLTSMDRDGTRSGFDLPLTRAIADSIQVPVIASGGVGNLDHLVDGIRDGHATAVLAASIFHFGEYTIRQA
KDHMVRCGLPMRLDP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=39.2996108949416, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=29.2887029288703, Blast_Score=101, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=312, Percent_Identity=32.3717948717949, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27389; Mature: 27389

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVRVIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAIAYDAAGADELCFLDITATHENRG
CEEEEEEEEECCCCCCEEECCCEEEECCCCCCHHEEEEECCCCCCCEEEEEEEEECCCCC
IMLDVVRRTAEACFMPVTVGGGVRTVDDIKTLLRSGADKVSINSAAVARREFVKEAAEKF
EEHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHH
GDQCIVVAIDAKRVPGRDRWEIFTHGGRKGTGIDAIEFAQEVVSLGAGEILLTSMDRDGT
CCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
RSGFDLPLTRAIADSIQVPVIASGGVGNLDHLVDGIRDGHATAVLAASIFHFGEYTIRQA
CCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KDHMVRCGLPMRLDP
HHHHEECCCCCCCCC
>Mature Secondary Structure
MFKVRVIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAIAYDAAGADELCFLDITATHENRG
CEEEEEEEEECCCCCCEEECCCEEEECCCCCCHHEEEEECCCCCCCEEEEEEEEECCCCC
IMLDVVRRTAEACFMPVTVGGGVRTVDDIKTLLRSGADKVSINSAAVARREFVKEAAEKF
EEHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHH
GDQCIVVAIDAKRVPGRDRWEIFTHGGRKGTGIDAIEFAQEVVSLGAGEILLTSMDRDGT
CCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
RSGFDLPLTRAIADSIQVPVIASGGVGNLDHLVDGIRDGHATAVLAASIFHFGEYTIRQA
CCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KDHMVRCGLPMRLDP
HHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA