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The map label for this gene is hisB

Identifier: 86747531

GI number: 86747531

Start: 450081

End: 450674

Strand: Direct

Name: hisB

Synonym: RPB_0405

Alternate gene names: 86747531

Gene position: 450081-450674 (Clockwise)

Preceding gene: 86747528

Following gene: 86747532

Centisome position: 8.44

GC content: 63.47

Gene sequence:

>594_bases
ATGCGCACGGCCACGATCAAGCGCAAAACCAAGGAAACCGACATCGAGGTGACCGTCAACCTCGATGGCGCCGGCGTGTC
CAATGCGGCGACCGGGATCGGCTTCTTCGACCATATGCTCGATCTGCTGGCCAAGCACTCCCGGATCGACATCACGGTGA
AGGCTGTGGGCGACCTGCATGTCGATTTCCACCACACCACCGAGGACGTCGGAATCGCGCTCGGCCAGGCGGTCAGGCAG
GCGCTCGGCAATATGGCGGGCATCATCCGCTATGCCAGCATGCTGATGCCGATGGACGAGACGCTGACCCGGGTGGTGAT
CGACGTTTCGGGCCGGCCGTTCCTGGTGTTCAAGGCGGAGTTTCCGCGTGACAAGATCGGCGAATTCGACACCGAGCTGG
TGCGCGAGTGGTTCCAGGCTTTCGCCATGAATGCGGGCGTGACTCTGCACGTCGAAACCCTATATGGCGAGAACAGCCAT
CATATCGCGGAATCCTGCTTCAAGGGTCTGGCGCGGGCGCTGCGCGCGGCGGTGGCGATCGATCCGCAGGCGGCGGGCGA
AGTGCCCTCGACCAAGGGTCAGCTCGGCGGCTAG

Upstream 100 bases:

>100_bases
ACCATAGGACGAAGATCGGGGCGCGGCCGCAGCCCCCGCAGTAGCGAAGGCGTCACCAGCCTGTTACAAGGACGGCGTTT
TTTACCGGAGAGCCCGCTTC

Downstream 100 bases:

>100_bases
GGCGCCGTCCATCGGCGGAGAAGGCAAAATGCCGGTCTACACAGTTCACGCGCCGCCGCCGGTCGACGACGAGCGTAGCG
CCAAACCGGACCGTTTCGTA

Product: imidazoleglycerol-phosphate dehydratase

Products: NA

Alternate protein names: IGPD

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MRTATIKRKTKETDIEVTVNLDGAGVSNAATGIGFFDHMLDLLAKHSRIDITVKAVGDLHVDFHHTTEDVGIALGQAVRQ
ALGNMAGIIRYASMLMPMDETLTRVVIDVSGRPFLVFKAEFPRDKIGEFDTELVREWFQAFAMNAGVTLHVETLYGENSH
HIAESCFKGLARALRAAVAIDPQAAGEVPSTKGQLGG

Sequences:

>Translated_197_residues
MRTATIKRKTKETDIEVTVNLDGAGVSNAATGIGFFDHMLDLLAKHSRIDITVKAVGDLHVDFHHTTEDVGIALGQAVRQ
ALGNMAGIIRYASMLMPMDETLTRVVIDVSGRPFLVFKAEFPRDKIGEFDTELVREWFQAFAMNAGVTLHVETLYGENSH
HIAESCFKGLARALRAAVAIDPQAAGEVPSTKGQLGG
>Mature_197_residues
MRTATIKRKTKETDIEVTVNLDGAGVSNAATGIGFFDHMLDLLAKHSRIDITVKAVGDLHVDFHHTTEDVGIALGQAVRQ
ALGNMAGIIRYASMLMPMDETLTRVVIDVSGRPFLVFKAEFPRDKIGEFDTELVREWFQAFAMNAGVTLHVETLYGENSH
HIAESCFKGLARALRAAVAIDPQAAGEVPSTKGQLGG

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the imidazoleglycerol-phosphate dehydratase family

Homologues:

Organism=Escherichia coli, GI87082027, Length=194, Percent_Identity=45.8762886597938, Blast_Score=177, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6324776, Length=218, Percent_Identity=42.2018348623853, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS7_RHOP2 (Q2J344)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484027.1
- ProteinModelPortal:   Q2J344
- SMR:   Q2J344
- STRING:   Q2J344
- GeneID:   3908843
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0405
- eggNOG:   COG0131
- HOGENOM:   HBG289010
- OMA:   TLHVETL
- ProtClustDB:   PRK00951
- BioCyc:   RPAL316058:RPB_0405-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00076
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR020568

Pfam domain/function: PF00475 IGPD; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =4.2.1.19

Molecular weight: Translated: 21377; Mature: 21377

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00954 IGP_DEHYDRATASE_1; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTATIKRKTKETDIEVTVNLDGAGVSNAATGIGFFDHMLDLLAKHSRIDITVKAVGDLH
CCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECEEE
VDFHHTTEDVGIALGQAVRQALGNMAGIIRYASMLMPMDETLTRVVIDVSGRPFLVFKAE
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEECCCEEEEEEEC
FPRDKIGEFDTELVREWFQAFAMNAGVTLHVETLYGENSHHIAESCFKGLARALRAAVAI
CCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
DPQAAGEVPSTKGQLGG
CCHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRTATIKRKTKETDIEVTVNLDGAGVSNAATGIGFFDHMLDLLAKHSRIDITVKAVGDLH
CCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECEEE
VDFHHTTEDVGIALGQAVRQALGNMAGIIRYASMLMPMDETLTRVVIDVSGRPFLVFKAE
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEECCCEEEEEEEC
FPRDKIGEFDTELVREWFQAFAMNAGVTLHVETLYGENSHHIAESCFKGLARALRAAVAI
CCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
DPQAAGEVPSTKGQLGG
CCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA