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The map label for this gene is mnmG

Identifier: 86747517

GI number: 86747517

Start: 434355

End: 436235

Strand: Reverse

Name: mnmG

Synonym: RPB_0391

Alternate gene names: 86747517

Gene position: 436235-434355 (Counterclockwise)

Preceding gene: 86747518

Following gene: 86747516

Centisome position: 8.18

GC content: 70.44

Gene sequence:

>1881_bases
ATGCGCAATTCCTACGATGTCATCGTCATTGGCGGCGGCCATGCCGGCTGCGAAGCCGCGGCTGCAGCGGCGCGGCTCGG
CGCCGCCACCGCGCTGGTGACGCATCGCTTCGCCACTGTCGGCGCGATGTCGTGCAATCCGGCGATCGGCGGTCTCGGCA
AGGGGCATCTGGTTCGCGAAGTCGACGCGCTGGACGGGCTGATGGGCCGGGTGGCCGATGCCGGCGGCATCCAGTTCCGG
ATGCTGAACCGGCGCAAGGGCCCGGCGGTGCGCGGACCGCGCGCCCAGGCCGACCGCAAGCTCTACGCCGCCGCGATGCA
GGCCGCGATCCGCGCCACCGCCAATCTCCGCGTGATCGAGGGCGAGGCGGACACGCTGCTGAACGAGGCGGGCCGGGTCA
CCGGGATTCGTCTGGCTGACGGCCGCGAGCTTGTGGCCGGCGCGGTCGTCATCACCACCGGCACCTTTTTGCGCGGGCTG
ATCCATCTCGGCGAACAGTGCTGGCCGGCCGGCCGGATCGATGAGGCGCCGGCGCTCGGGCTGTCGGCCTCGTTCGAGGC
GCTGGGTCTCGCGCTCGGCCGCCTCAAGACCGGGACGCCGCCGCGGCTCGACGGCCGCACCATCGACTGGGGCGCGGTCG
AGATGCAGCCCGGCGACGATCCGCCGGAGCCGTTCTCGGTGCTGACGCCGGCGATCACCACGCCGCAGATCGAATGCGGC
ATCACCCGGACGCTGCCCGCGACCCACGAGGTGATCCGCGCCAATGTGCATCGCTCGCCGATGTATTCCGGGCAGATCCA
GTCGTCGGGGCCGCGCTATTGCCCGTCGATCGAGGACAAGATCGTCAAATTCGGCGACCGCGACGGCCACCAGATCTTCC
TCGAGCCGGAAGGGCTCGACGATCCGACCGTCTATCCCAACGGGATCTCGACCTCGCTGCCCGAAGAGGTGCAGCGCGCG
ATCCTGGCCACGATCCCGGGCCTGGAGCGCACCGTGATGCTGCGGCCGGGCTATGCGATCGAATACGATCATGTCGATCC
GCGCGAGCTGGAGCCGACGTTGCAGACCAAGCGGTTGCGCGGGCTGTATCTGGCCGGCCAGATCAACGGCACCACCGGCT
ATGAAGAGGCCGCGGCGCAGGGCCTGGTCGCCGGGTTGAACGCGGCGCTGGCGGCCGGCGGCGGCGAGGGCGTCGTGTTC
GACCGCGCCGACGGCTATCTCGGGGTGATGATCGACGATCTGGTGACCCGCGGTATCTCCGAGCCGTACCGGATGTTCAC
TTCCCGGGCGGAGTATCGCCTGACGCTGCGGGCCGACAATGCCGATCAGCGGCTGACCGACAAGGGTCTGGCGCTGGGCT
GCGTCGGGACGGAGCGGGCGGCGTTCCACCGCGGCAAAATGGCGGCGCTGGCCGAGGCCAAGGCGCTGGCGCAGTCGCTG
GCGATCACGCCGAACGAGGCGGCCAGGCATGGGCTGTCGCTCAATCGCGACGGCCAGCGGCGCTCGGCGTTCGATCTGCT
GTCCTATCCGGAGATCGAATGGCCGCAGGTGAGGGCGATCTGGCCGGAACTGGCGGCGGTTTCGCCGTCGATCGCCGGGC
ATGTCGAGATCGACGCCAAGTATGCCGTCTATCTGGAGCGCCAGGTCGCCGACGTCGCCGCGTTCCGGCGCGATGAGGGC
CTGCTGCTGACCGATGTCGACTATGCGCAGGTGCCCGGACTGTCGAACGAGGCGCGGGGCCGGCTGGAGCGGCACCGGCC
ACGCACGGTCGGGCAGGCGGGTCGCCTGGACGGTATCACCCCCGCGGCGCTCGGCATTCTGGCGGCCTATCTGCGGCGAG
AAGCGCGGAAGCGGCCGGCGGTGGTGGACGGCGAAGGCTAG

Upstream 100 bases:

>100_bases
GGGGCTGATTCCCGGAGACCAACGCACTCGCGTTTCACGTGAAACACGGCCGAATCCAATACCGCTTCAAAGCCGGGAAA
AGCTGCGCTACAAGCCGGCC

Downstream 100 bases:

>100_bases
GCGAGGCCGCTGTTTCACGTGAAACAGCGCGCCGCCTCGTTTGCGGGGCCGGATCGGCTCGTTGGGCGCCTTGAAGCTCG
TCGATCGACCGGCTTGGGCA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MRNSYDVIVIGGGHAGCEAAAAAARLGAATALVTHRFATVGAMSCNPAIGGLGKGHLVREVDALDGLMGRVADAGGIQFR
MLNRRKGPAVRGPRAQADRKLYAAAMQAAIRATANLRVIEGEADTLLNEAGRVTGIRLADGRELVAGAVVITTGTFLRGL
IHLGEQCWPAGRIDEAPALGLSASFEALGLALGRLKTGTPPRLDGRTIDWGAVEMQPGDDPPEPFSVLTPAITTPQIECG
ITRTLPATHEVIRANVHRSPMYSGQIQSSGPRYCPSIEDKIVKFGDRDGHQIFLEPEGLDDPTVYPNGISTSLPEEVQRA
ILATIPGLERTVMLRPGYAIEYDHVDPRELEPTLQTKRLRGLYLAGQINGTTGYEEAAAQGLVAGLNAALAAGGGEGVVF
DRADGYLGVMIDDLVTRGISEPYRMFTSRAEYRLTLRADNADQRLTDKGLALGCVGTERAAFHRGKMAALAEAKALAQSL
AITPNEAARHGLSLNRDGQRRSAFDLLSYPEIEWPQVRAIWPELAAVSPSIAGHVEIDAKYAVYLERQVADVAAFRRDEG
LLLTDVDYAQVPGLSNEARGRLERHRPRTVGQAGRLDGITPAALGILAAYLRREARKRPAVVDGEG

Sequences:

>Translated_626_residues
MRNSYDVIVIGGGHAGCEAAAAAARLGAATALVTHRFATVGAMSCNPAIGGLGKGHLVREVDALDGLMGRVADAGGIQFR
MLNRRKGPAVRGPRAQADRKLYAAAMQAAIRATANLRVIEGEADTLLNEAGRVTGIRLADGRELVAGAVVITTGTFLRGL
IHLGEQCWPAGRIDEAPALGLSASFEALGLALGRLKTGTPPRLDGRTIDWGAVEMQPGDDPPEPFSVLTPAITTPQIECG
ITRTLPATHEVIRANVHRSPMYSGQIQSSGPRYCPSIEDKIVKFGDRDGHQIFLEPEGLDDPTVYPNGISTSLPEEVQRA
ILATIPGLERTVMLRPGYAIEYDHVDPRELEPTLQTKRLRGLYLAGQINGTTGYEEAAAQGLVAGLNAALAAGGGEGVVF
DRADGYLGVMIDDLVTRGISEPYRMFTSRAEYRLTLRADNADQRLTDKGLALGCVGTERAAFHRGKMAALAEAKALAQSL
AITPNEAARHGLSLNRDGQRRSAFDLLSYPEIEWPQVRAIWPELAAVSPSIAGHVEIDAKYAVYLERQVADVAAFRRDEG
LLLTDVDYAQVPGLSNEARGRLERHRPRTVGQAGRLDGITPAALGILAAYLRREARKRPAVVDGEG
>Mature_626_residues
MRNSYDVIVIGGGHAGCEAAAAAARLGAATALVTHRFATVGAMSCNPAIGGLGKGHLVREVDALDGLMGRVADAGGIQFR
MLNRRKGPAVRGPRAQADRKLYAAAMQAAIRATANLRVIEGEADTLLNEAGRVTGIRLADGRELVAGAVVITTGTFLRGL
IHLGEQCWPAGRIDEAPALGLSASFEALGLALGRLKTGTPPRLDGRTIDWGAVEMQPGDDPPEPFSVLTPAITTPQIECG
ITRTLPATHEVIRANVHRSPMYSGQIQSSGPRYCPSIEDKIVKFGDRDGHQIFLEPEGLDDPTVYPNGISTSLPEEVQRA
ILATIPGLERTVMLRPGYAIEYDHVDPRELEPTLQTKRLRGLYLAGQINGTTGYEEAAAQGLVAGLNAALAAGGGEGVVF
DRADGYLGVMIDDLVTRGISEPYRMFTSRAEYRLTLRADNADQRLTDKGLALGCVGTERAAFHRGKMAALAEAKALAQSL
AITPNEAARHGLSLNRDGQRRSAFDLLSYPEIEWPQVRAIWPELAAVSPSIAGHVEIDAKYAVYLERQVADVAAFRRDEG
LLLTDVDYAQVPGLSNEARGRLERHRPRTVGQAGRLDGITPAALGILAAYLRREARKRPAVVDGEG

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=639, Percent_Identity=44.131455399061, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI19882217, Length=664, Percent_Identity=42.4698795180723, Blast_Score=496, Evalue=1e-140,
Organism=Homo sapiens, GI183227703, Length=679, Percent_Identity=41.5316642120766, Blast_Score=491, Evalue=1e-139,
Organism=Escherichia coli, GI2367273, Length=625, Percent_Identity=50.72, Blast_Score=590, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI17534255, Length=629, Percent_Identity=44.0381558028617, Blast_Score=494, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6321202, Length=638, Percent_Identity=46.0815047021944, Blast_Score=545, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24658174, Length=629, Percent_Identity=46.5818759936407, Blast_Score=525, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_RHOP2 (Q2J358)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484013.1
- ProteinModelPortal:   Q2J358
- SMR:   Q2J358
- STRING:   Q2J358
- GeneID:   3908829
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0391
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   RPAL316058:RPB_0391-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 66861; Mature: 66861

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSYDVIVIGGGHAGCEAAAAAARLGAATALVTHRFATVGAMSCNPAIGGLGKGHLVRE
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
VDALDGLMGRVADAGGIQFRMLNRRKGPAVRGPRAQADRKLYAAAMQAAIRATANLRVIE
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEE
GEADTLLNEAGRVTGIRLADGRELVAGAVVITTGTFLRGLIHLGEQCWPAGRIDEAPALG
CCHHHHHHHCCCEEEEEEECCCEEHEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LSASFEALGLALGRLKTGTPPRLDGRTIDWGAVEMQPGDDPPEPFSVLTPAITTPQIECG
CCCCHHHHHHHHHHCCCCCCCCCCCCEECCCEEEECCCCCCCCCHHHHCCCCCCCCEECC
ITRTLPATHEVIRANVHRSPMYSGQIQSSGPRYCPSIEDKIVKFGDRDGHQIFLEPEGLD
CEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCC
DPTVYPNGISTSLPEEVQRAILATIPGLERTVMLRPGYAIEYDHVDPRELEPTLQTKRLR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHCCCHHHHHHHC
GLYLAGQINGTTGYEEAAAQGLVAGLNAALAAGGGEGVVFDRADGYLGVMIDDLVTRGIS
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHCCC
EPYRMFTSRAEYRLTLRADNADQRLTDKGLALGCVGTERAAFHRGKMAALAEAKALAQSL
HHHHHHHCCCEEEEEEEECCCCHHHCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHH
AITPNEAARHGLSLNRDGQRRSAFDLLSYPEIEWPQVRAIWPELAAVSPSIAGHVEIDAK
CCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCE
YAVYLERQVADVAAFRRDEGLLLTDVDYAQVPGLSNEARGRLERHRPRTVGQAGRLDGIT
EEEEEHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
PAALGILAAYLRREARKRPAVVDGEG
HHHHHHHHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure
MRNSYDVIVIGGGHAGCEAAAAAARLGAATALVTHRFATVGAMSCNPAIGGLGKGHLVRE
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
VDALDGLMGRVADAGGIQFRMLNRRKGPAVRGPRAQADRKLYAAAMQAAIRATANLRVIE
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEE
GEADTLLNEAGRVTGIRLADGRELVAGAVVITTGTFLRGLIHLGEQCWPAGRIDEAPALG
CCHHHHHHHCCCEEEEEEECCCEEHEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LSASFEALGLALGRLKTGTPPRLDGRTIDWGAVEMQPGDDPPEPFSVLTPAITTPQIECG
CCCCHHHHHHHHHHCCCCCCCCCCCCEECCCEEEECCCCCCCCCHHHHCCCCCCCCEECC
ITRTLPATHEVIRANVHRSPMYSGQIQSSGPRYCPSIEDKIVKFGDRDGHQIFLEPEGLD
CEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCC
DPTVYPNGISTSLPEEVQRAILATIPGLERTVMLRPGYAIEYDHVDPRELEPTLQTKRLR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHCCCHHHHHHHC
GLYLAGQINGTTGYEEAAAQGLVAGLNAALAAGGGEGVVFDRADGYLGVMIDDLVTRGIS
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHCCC
EPYRMFTSRAEYRLTLRADNADQRLTDKGLALGCVGTERAAFHRGKMAALAEAKALAQSL
HHHHHHHCCCEEEEEEEECCCCHHHCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHH
AITPNEAARHGLSLNRDGQRRSAFDLLSYPEIEWPQVRAIWPELAAVSPSIAGHVEIDAK
CCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCE
YAVYLERQVADVAAFRRDEGLLLTDVDYAQVPGLSNEARGRLERHRPRTVGQAGRLDGIT
EEEEEHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
PAALGILAAYLRREARKRPAVVDGEG
HHHHHHHHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA