Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 86747516

GI number: 86747516

Start: 433056

End: 433769

Strand: Reverse

Name: gidB

Synonym: RPB_0390

Alternate gene names: 86747516

Gene position: 433769-433056 (Counterclockwise)

Preceding gene: 86747517

Following gene: 86747515

Centisome position: 8.14

GC content: 66.11

Gene sequence:

>714_bases
ATGGCCTCGCGACATTCGCCGCAGACAGCCGCCCAGCCCGACGCTGCCGACAAGGCGCAGGCACTGAGGCTCACCCCCGT
TTCACGTGAAACAGAGACGCGGCTCGATGCCTATCTGGATCTGCTCCGCCTGTGGCAGGCCAAGACCAATCTGGTGGCGC
CGTCGACGCTGCCGCAGCTCTGGACCCGGCATGTCGCCGACTCGCTTCAGCTCCTTGCGCTCGCGCCGACGGCGCGGCGC
TGGCTCGATTTCGGGAGCGGCGGCGGCTTCCCCGGCATCGTGCTGGCCTGCGCAATGGCCGAGCACGACGGCGGCCACGT
CACGCTGGTCGAGCGCAACGCCAAGAAGGCCGCTTTCTTGCGCGAGGCGCTGCGCGTCACCGGCGCGCCGGGCACAGTTA
TGCTCGCGGACATTGGGGATAACGTGGATAGGTTCCCGCGGGCGCTCGATTGCATCACCGCGCGTGCCGTTGCTCCGCTA
CACCAGCTCATCGGTTTCGCCGCGCCGCTGATGACCGAGGGCAGCAAGGTGCTGTTTCTCAAGGGTCAAGATGTAGAAGC
GGAATTGACGGAAGCTACTAAGTATTGGAAGATCGAGCCCCAATTGCACGCCAGCCTGACCGGCGGGCAGGGCTGGATCG
TCGAACTCGACCGCATCGTCCGACACGCCCCCTCCACCACCAACCCAGAAGCCGCCGCGCATGACCGTCATTGA

Upstream 100 bases:

>100_bases
ATCGCAATCACTCCCGGCGCCCGACCAAACAGTGGCTGGAATCGCCGCGGCGGCTTATGTGTAATCCACACCGATCCTCG
AACCCCCGAGACCCCGGCCG

Downstream 100 bases:

>100_bases
TGAAATATTTCAAGGGGATAGGGCGATGACCGAGCCTGGACGCCCACGCATCATCGCGCTCGCCAACCAGAAGGGCGGGG
TCGGCAAGACCACCACGGCG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MASRHSPQTAAQPDAADKAQALRLTPVSRETETRLDAYLDLLRLWQAKTNLVAPSTLPQLWTRHVADSLQLLALAPTARR
WLDFGSGGGFPGIVLACAMAEHDGGHVTLVERNAKKAAFLREALRVTGAPGTVMLADIGDNVDRFPRALDCITARAVAPL
HQLIGFAAPLMTEGSKVLFLKGQDVEAELTEATKYWKIEPQLHASLTGGQGWIVELDRIVRHAPSTTNPEAAAHDRH

Sequences:

>Translated_237_residues
MASRHSPQTAAQPDAADKAQALRLTPVSRETETRLDAYLDLLRLWQAKTNLVAPSTLPQLWTRHVADSLQLLALAPTARR
WLDFGSGGGFPGIVLACAMAEHDGGHVTLVERNAKKAAFLREALRVTGAPGTVMLADIGDNVDRFPRALDCITARAVAPL
HQLIGFAAPLMTEGSKVLFLKGQDVEAELTEATKYWKIEPQLHASLTGGQGWIVELDRIVRHAPSTTNPEAAAHDRH
>Mature_236_residues
ASRHSPQTAAQPDAADKAQALRLTPVSRETETRLDAYLDLLRLWQAKTNLVAPSTLPQLWTRHVADSLQLLALAPTARRW
LDFGSGGGFPGIVLACAMAEHDGGHVTLVERNAKKAAFLREALRVTGAPGTVMLADIGDNVDRFPRALDCITARAVAPLH
QLIGFAAPLMTEGSKVLFLKGQDVEAELTEATKYWKIEPQLHASLTGGQGWIVELDRIVRHAPSTTNPEAAAHDRH

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=170, Percent_Identity=27.6470588235294, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_RHOP2 (Q2J359)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484012.1
- ProteinModelPortal:   Q2J359
- SMR:   Q2J359
- STRING:   Q2J359
- GeneID:   3908828
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0390
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   GGQGWIV
- ProtClustDB:   PRK00107
- BioCyc:   RPAL316058:RPB_0390-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 25713; Mature: 25581

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRHSPQTAAQPDAADKAQALRLTPVSRETETRLDAYLDLLRLWQAKTNLVAPSTLPQL
CCCCCCCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
WTRHVADSLQLLALAPTARRWLDFGSGGGFPGIVLACAMAEHDGGHVTLVERNAKKAAFL
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHH
REALRVTGAPGTVMLADIGDNVDRFPRALDCITARAVAPLHQLIGFAAPLMTEGSKVLFL
HHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KGQDVEAELTEATKYWKIEPQLHASLTGGQGWIVELDRIVRHAPSTTNPEAAAHDRH
ECCCCCHHHHHHHHEEEECCCEEEEEECCCCCEEEHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASRHSPQTAAQPDAADKAQALRLTPVSRETETRLDAYLDLLRLWQAKTNLVAPSTLPQL
CCCCCCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
WTRHVADSLQLLALAPTARRWLDFGSGGGFPGIVLACAMAEHDGGHVTLVERNAKKAAFL
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHH
REALRVTGAPGTVMLADIGDNVDRFPRALDCITARAVAPLHQLIGFAAPLMTEGSKVLFL
HHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KGQDVEAELTEATKYWKIEPQLHASLTGGQGWIVELDRIVRHAPSTTNPEAAAHDRH
ECCCCCHHHHHHHHEEEECCCEEEEEECCCCCEEEHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA