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The map label for this gene is ubiF [C]

Identifier: 86747495

GI number: 86747495

Start: 409745

End: 410965

Strand: Reverse

Name: ubiF [C]

Synonym: RPB_0369

Alternate gene names: 86747495

Gene position: 410965-409745 (Counterclockwise)

Preceding gene: 86747506

Following gene: 86747494

Centisome position: 7.71

GC content: 70.43

Gene sequence:

>1221_bases
ATGACCGCACCGCGAAGCATTGTGATCGGAGGCGGCGCCTTCGCCGGTCTGGCGCTGGCGCTGGCGCTGCGCCAGGGGCT
CGGCCCCGAGGTCCCGATTGTTGTCGCCGACCCGGCGCTGGCGATGCGGCCGTCGCGCGATCCGCGCGCCACCGCGATCG
TCGCCGCCTGTCGCCGGCTGTTCGAGGCGCTCGGCGTCTGGGACGAGGTGGCGCCGACGGCGCAGCCGATCATCGACATG
GTGGTGACGGATTCGGCGCTCGAGGACGCGACCCGGCCGGTGTTCCTGACCTTCGCCGGCGAAGTCCAGCCGGGCGAGCC
GTTCGCCCATATGGTCGAGAACAAATATCTCAACGAGGCGCTGGCCAGCCGCGCCGAGGCCGCCGGCGTCGAGCTGCGCG
CCACGCCGGTGACATCGTACCAGGCGGGCGATGACGCGACCCGCGTCACGCTCGGCGATGGCAGCGTGATCGACGCCAGC
CTGCTGGTCGCCGCCGACGGCGCCAAGTCGAAGCTGCGCGAACGCGCCGGCATTGCCACCTATGGCTGGGACTACGACCA
GTCCGGCATCGTCGTCATGGTCGAGCACGAGCGCGACCACCATGGCTGCGCCGAGGAGCATTTTTTGCCCGCGGGGCCGT
TTGCGATCCTGCCGCTGACCGGCCGCCGCTCGTCGCTGGTGTGGACCGAAAGCCGCGCCGACGCGGCCAAGATCGTGGCG
CTGTCCGCCAAGGAATTCCAGGCCGAGCTGGAGACGCGGTTCGGGCTGCGGCTCGGCGAGGTCAAGCCGCTGGATGCGCC
CAAGGCGTTTCCGCTCGGCTATTTCGTGGCGCAATCCTTCATCGCGCCGCGGCTGGCGCTGATCGGCGACGCCGCCCATG
TGATTCACCCGATCGCCGGGCAGGGCCTCAATATGGGCCTGCGCGACGTGGCGGCGCTGGCCGAAGTGGTGGTCGATGCG
GCGCGGCTCGGGATCGATCCGGGACAAGCCGACGTGCTGGAGAACTACCAGCGCTGGCGGCGGTTCGACACCATGGCGAT
GGGCGTCGCCACCAACGGGCTGAACCTGCTGTTCTCCAACAAATCGCCGCTGCTGCGCAGCGTCCGCGACATCGGCCTCG
GCATCGTCGACCGGCTGCCGCCGCTGAAGGACGTCTTCATCCGCCAGGCCGCCGGTCTCACCGGCGACACCCCGCGCCTG
CTGAAGGGCGAGGCGCTGTAG

Upstream 100 bases:

>100_bases
AGAACCGGGCGGCAAGCCATGGCTGTTCCTGCTTGTTTCGCCCACCTCCAGCCATGGCGCAAGAACACAGACAAGCGACT
CTGGCGCACGAGTGAGAAAA

Downstream 100 bases:

>100_bases
ACGGCTCTGCGGGCCGAACCATCAGGTCGCGGCCGAAAACTGGACCATGGACCGGTCGACCGGCGCCTGTGAGCTCCTGG
CGGCCTCTTGTTGTCCCAGA

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTAPRSIVIGGGAFAGLALALALRQGLGPEVPIVVADPALAMRPSRDPRATAIVAACRRLFEALGVWDEVAPTAQPIIDM
VVTDSALEDATRPVFLTFAGEVQPGEPFAHMVENKYLNEALASRAEAAGVELRATPVTSYQAGDDATRVTLGDGSVIDAS
LLVAADGAKSKLRERAGIATYGWDYDQSGIVVMVEHERDHHGCAEEHFLPAGPFAILPLTGRRSSLVWTESRADAAKIVA
LSAKEFQAELETRFGLRLGEVKPLDAPKAFPLGYFVAQSFIAPRLALIGDAAHVIHPIAGQGLNMGLRDVAALAEVVVDA
ARLGIDPGQADVLENYQRWRRFDTMAMGVATNGLNLLFSNKSPLLRSVRDIGLGIVDRLPPLKDVFIRQAAGLTGDTPRL
LKGEAL

Sequences:

>Translated_406_residues
MTAPRSIVIGGGAFAGLALALALRQGLGPEVPIVVADPALAMRPSRDPRATAIVAACRRLFEALGVWDEVAPTAQPIIDM
VVTDSALEDATRPVFLTFAGEVQPGEPFAHMVENKYLNEALASRAEAAGVELRATPVTSYQAGDDATRVTLGDGSVIDAS
LLVAADGAKSKLRERAGIATYGWDYDQSGIVVMVEHERDHHGCAEEHFLPAGPFAILPLTGRRSSLVWTESRADAAKIVA
LSAKEFQAELETRFGLRLGEVKPLDAPKAFPLGYFVAQSFIAPRLALIGDAAHVIHPIAGQGLNMGLRDVAALAEVVVDA
ARLGIDPGQADVLENYQRWRRFDTMAMGVATNGLNLLFSNKSPLLRSVRDIGLGIVDRLPPLKDVFIRQAAGLTGDTPRL
LKGEAL
>Mature_405_residues
TAPRSIVIGGGAFAGLALALALRQGLGPEVPIVVADPALAMRPSRDPRATAIVAACRRLFEALGVWDEVAPTAQPIIDMV
VTDSALEDATRPVFLTFAGEVQPGEPFAHMVENKYLNEALASRAEAAGVELRATPVTSYQAGDDATRVTLGDGSVIDASL
LVAADGAKSKLRERAGIATYGWDYDQSGIVVMVEHERDHHGCAEEHFLPAGPFAILPLTGRRSSLVWTESRADAAKIVAL
SAKEFQAELETRFGLRLGEVKPLDAPKAFPLGYFVAQSFIAPRLALIGDAAHVIHPIAGQGLNMGLRDVAALAEVVVDAA
RLGIDPGQADVLENYQRWRRFDTMAMGVATNGLNLLFSNKSPLLRSVRDIGLGIVDRLPPLKDVFIRQAAGLTGDTPRLL
KGEAL

Specific function: Oxygenase That Introduces The Hydroxyl Group At Carbon Five Of 2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol Resulting In The Formation Of 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=381, Percent_Identity=29.3963254593176, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI32967307, Length=320, Percent_Identity=32.1875, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1789273, Length=403, Percent_Identity=36.2282878411911, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1786883, Length=397, Percent_Identity=38.7909319899244, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1789274, Length=392, Percent_Identity=33.6734693877551, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI25146631, Length=434, Percent_Identity=28.5714285714286, Blast_Score=145, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6321694, Length=294, Percent_Identity=28.9115646258503, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920760, Length=384, Percent_Identity=29.1666666666667, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 43218; Mature: 43087

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPRSIVIGGGAFAGLALALALRQGLGPEVPIVVADPALAMRPSRDPRATAIVAACRRL
CCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCCCCCCCHHHHHHHHHHHH
FEALGVWDEVAPTAQPIIDMVVTDSALEDATRPVFLTFAGEVQPGEPFAHMVENKYLNEA
HHHHCCHHHHCCCHHHHHHHHHCCHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
LASRAEAAGVELRATPVTSYQAGDDATRVTLGDGSVIDASLLVAADGAKSKLRERAGIAT
HHHHHHHCCCEEEECCCCCCCCCCCCEEEEECCCCEEEEEEEEEECCHHHHHHHHCCCEE
YGWDYDQSGIVVMVEHERDHHGCAEEHFLPAGPFAILPLTGRRSSLVWTESRADAAKIVA
ECCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCCEEE
LSAKEFQAELETRFGLRLGEVKPLDAPKAFPLGYFVAQSFIAPRLALIGDAAHVIHPIAG
ECHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
QGLNMGLRDVAALAEVVVDAARLGIDPGQADVLENYQRWRRFDTMAMGVATNGLNLLFSN
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEECC
KSPLLRSVRDIGLGIVDRLPPLKDVFIRQAAGLTGDTPRLLKGEAL
CCHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAPRSIVIGGGAFAGLALALALRQGLGPEVPIVVADPALAMRPSRDPRATAIVAACRRL
CCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCCCCCCCHHHHHHHHHHHH
FEALGVWDEVAPTAQPIIDMVVTDSALEDATRPVFLTFAGEVQPGEPFAHMVENKYLNEA
HHHHCCHHHHCCCHHHHHHHHHCCHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
LASRAEAAGVELRATPVTSYQAGDDATRVTLGDGSVIDASLLVAADGAKSKLRERAGIAT
HHHHHHHCCCEEEECCCCCCCCCCCCEEEEECCCCEEEEEEEEEECCHHHHHHHHCCCEE
YGWDYDQSGIVVMVEHERDHHGCAEEHFLPAGPFAILPLTGRRSSLVWTESRADAAKIVA
ECCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCCEEE
LSAKEFQAELETRFGLRLGEVKPLDAPKAFPLGYFVAQSFIAPRLALIGDAAHVIHPIAG
ECHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
QGLNMGLRDVAALAEVVVDAARLGIDPGQADVLENYQRWRRFDTMAMGVATNGLNLLFSN
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEECC
KSPLLRSVRDIGLGIVDRLPPLKDVFIRQAAGLTGDTPRLLKGEAL
CCHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]