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The map label for this gene is mcl2 [H]

Identifier: 86747467

GI number: 86747467

Start: 386284

End: 387162

Strand: Direct

Name: mcl2 [H]

Synonym: RPB_0341

Alternate gene names: 86747467

Gene position: 386284-387162 (Clockwise)

Preceding gene: 86747465

Following gene: 86747470

Centisome position: 7.25

GC content: 70.31

Gene sequence:

>879_bases
ATGATCCGACCGCGCCGCAGCCTGTTGTTCATGCCGGGGTCGAATGCGCGCGCGCTGGAGAAGGCCCGCCTGCTGCCGGC
CGACGGCCTGATCCTCGACCTCGAGGACGCCGTGGCGCCGGACGCCAAGGCCGCCGCGCGCGACCAGATCGCAACCGCGG
TCGCCGCCAAGGGATACGGCCACCGCGAGGTGCTGGTGCGGATCAATGCGCTGGATTCGCCGTGGTGGCGCGACGACATC
GCGATGGCCGCCAAGGTCGGCCCCGACGGTATCCTGGTTCCGAAAGTGTCGAGCATCGAGGATATCGCCGCCATCGCCGA
GCGCCTCACCGAGCTCGACGCCGATCCGGCGATCCGGGTCTGGATCATGATCGAGACCGCGCGCGGCGTGTTGCACGCCG
AGGAGCTGGCGGCGCAGACCCACATCGCCGGCACCCGGCTCGCCGGCTTCGTGTTCGGGCCGAACGACATTTCACGCGAG
ACCGGCATCCGGATGCTACCGGGCCGCGCCACGATGATTCCGATGATCAGCCACTGCATCCTCGCCGCCCGCGCGCACGG
CCTCGTGGTGCTCGACGGCCCCTACAGCGACATCGCCAATGTCGACGGCTTCGCCCAGGAATGCGCCCAGGCGCGCGATC
TCGGCTTCGACGGCAAGACGCTGATCCATCCCGGCCAGATCGACGCCTGCAACGCCACCTTCACGCCGCCGGCGGAGGAC
GTGGCGCAGGCCCGCGCGATCCTCGCCGCCTTCGAGCGCCCGGAAAATGCCGGCCGCGGCGCGATCCAGCTCGACGGCCG
CATGGTCGAGCGCCTGCACGCCGAAATCGCCAAAAAGACCATCGCGATCGCCGACGCGATCGAGCGGGCGGGGCGGTGA

Upstream 100 bases:

>100_bases
CCCGCCCGCATAATCACAGGATGCCGCCGCGAACAAGTCTTCCGGCGCGCAGGCCGAGATGATATCGCCATCGTTTTCCG
CGCACCGAGGGAACAGACCG

Downstream 100 bases:

>100_bases
TTTCTACCCACCAACGTCATCGCCGGGCTCGACCCGGCGATCCACCATCTTCGTAAGAAGATGGATGCGCGGGTCAAGCC
CGCGCATGACGGCTGAGACA

Product: Citryl-CoA lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MIRPRRSLLFMPGSNARALEKARLLPADGLILDLEDAVAPDAKAAARDQIATAVAAKGYGHREVLVRINALDSPWWRDDI
AMAAKVGPDGILVPKVSSIEDIAAIAERLTELDADPAIRVWIMIETARGVLHAEELAAQTHIAGTRLAGFVFGPNDISRE
TGIRMLPGRATMIPMISHCILAARAHGLVVLDGPYSDIANVDGFAQECAQARDLGFDGKTLIHPGQIDACNATFTPPAED
VAQARAILAAFERPENAGRGAIQLDGRMVERLHAEIAKKTIAIADAIERAGR

Sequences:

>Translated_292_residues
MIRPRRSLLFMPGSNARALEKARLLPADGLILDLEDAVAPDAKAAARDQIATAVAAKGYGHREVLVRINALDSPWWRDDI
AMAAKVGPDGILVPKVSSIEDIAAIAERLTELDADPAIRVWIMIETARGVLHAEELAAQTHIAGTRLAGFVFGPNDISRE
TGIRMLPGRATMIPMISHCILAARAHGLVVLDGPYSDIANVDGFAQECAQARDLGFDGKTLIHPGQIDACNATFTPPAED
VAQARAILAAFERPENAGRGAIQLDGRMVERLHAEIAKKTIAIADAIERAGR
>Mature_292_residues
MIRPRRSLLFMPGSNARALEKARLLPADGLILDLEDAVAPDAKAAARDQIATAVAAKGYGHREVLVRINALDSPWWRDDI
AMAAKVGPDGILVPKVSSIEDIAAIAERLTELDADPAIRVWIMIETARGVLHAEELAAQTHIAGTRLAGFVFGPNDISRE
TGIRMLPGRATMIPMISHCILAARAHGLVVLDGPYSDIANVDGFAQECAQARDLGFDGKTLIHPGQIDACNATFTPPAED
VAQARAILAAFERPENAGRGAIQLDGRMVERLHAEIAKKTIAIADAIERAGR

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=295, Percent_Identity=27.7966101694915, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI87081764, Length=273, Percent_Identity=34.4322344322344, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17557458, Length=285, Percent_Identity=32.6315789473684, Blast_Score=129, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 31247; Mature: 31247

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPRRSLLFMPGSNARALEKARLLPADGLILDLEDAVAPDAKAAARDQIATAVAAKGYG
CCCCCCCEEEECCCCCHHHHHHHCCCCCCEEEECHHHCCCCHHHHHHHHHHHHHHHCCCC
HREVLVRINALDSPWWRDDIAMAAKVGPDGILVPKVSSIEDIAAIAERLTELDADPAIRV
CEEEEEEEECCCCCCCHHHHHHHEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEE
WIMIETARGVLHAEELAAQTHIAGTRLAGFVFGPNDISRETGIRMLPGRATMIPMISHCI
EEEEECCCCHHHHHHHHHHHHHCHHHEEEEEECCCCCCHHCCCEECCCCCHHHHHHHHHH
LAARAHGLVVLDGPYSDIANVDGFAQECAQARDLGFDGKTLIHPGQIDACNATFTPPAED
HHHHCCCEEEECCCCHHHHCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCHHH
VAQARAILAAFERPENAGRGAIQLDGRMVERLHAEIAKKTIAIADAIERAGR
HHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRPRRSLLFMPGSNARALEKARLLPADGLILDLEDAVAPDAKAAARDQIATAVAAKGYG
CCCCCCCEEEECCCCCHHHHHHHCCCCCCEEEECHHHCCCCHHHHHHHHHHHHHHHCCCC
HREVLVRINALDSPWWRDDIAMAAKVGPDGILVPKVSSIEDIAAIAERLTELDADPAIRV
CEEEEEEEECCCCCCCHHHHHHHEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEE
WIMIETARGVLHAEELAAQTHIAGTRLAGFVFGPNDISRETGIRMLPGRATMIPMISHCI
EEEEECCCCHHHHHHHHHHHHHCHHHEEEEEECCCCCCHHCCCEECCCCCHHHHHHHHHH
LAARAHGLVVLDGPYSDIANVDGFAQECAQARDLGFDGKTLIHPGQIDACNATFTPPAED
HHHHCCCEEEECCCCHHHHCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCHHH
VAQARAILAAFERPENAGRGAIQLDGRMVERLHAEIAKKTIAIADAIERAGR
HHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA