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The map label for this gene is poxB [H]

Identifier: 86747329

GI number: 86747329

Start: 226211

End: 227941

Strand: Direct

Name: poxB [H]

Synonym: RPB_0203

Alternate gene names: 86747329

Gene position: 226211-227941 (Clockwise)

Preceding gene: 86747322

Following gene: 86747330

Centisome position: 4.24

GC content: 65.22

Gene sequence:

>1731_bases
ATGTCCAATGTTGCTGATCGAATTGTCGAAACCCTGCATCAAGCCGGCGTCGAGCGGATCTTCGGCGTGGTCGGAGACAG
CCTCAACGGACTGACGGAAGCGTTGCGCCAGCACGGCAGCATCGAATGGGTGCACGTTCGCCACGAAGAAGTCGCTGCTT
TCGCGGCCGCCGGGGAATCGCAGATCACCGGCAAGCTCGCGGTTTGCGCCGGATCCTGCGGTCCGGGAAACCTGCATCTG
ATCAATGGCCTGTATGATGCACAGCGGACCCGGACTCCGGTGCTGGCCATCGCGGCGCAGATTCCATCGGCGGAGATCGG
CGGCGGCTACTTCCAGGAAACGCACCCGCAGAATCTGTTTCGCGAATGCAGCGTCTATTGCGAGCTGGTGTCCGATCCGC
ATCAGTTCGACTACGTCCTCGAAAACGCGATCCGGGCCGCGGTCGGTCAGCGGGGTGTCGCCGTCGTCGTGATTCCTGGC
GATGTGGCGCTGCGCGAGGCTTCGACACGTGGGGTGACGCCCGTTGCCGGCCTGCTGCCGCCGACGCCGATCGTGACGCC
GGCCGAGCCGCAGCTCGACGCGCTGGCGGCGCTGCTGAACGGCGCCGGGCGGGTCACGCTGTTCTGCGGCCGCGGCTGTG
CAGGTGCCCACGCGCCGTTGATGGCGCTGGCCGAAGCTCTGAAGAGCCCGATCGTCCACGCGCTGGGCGGCAAGGAACAC
GTCGAATACGACAATCCCTACGACGTCGGGATGACCGGCTTCATCGGCTACGCGTCGGGCTACGAGGCGATGCATGCCTG
CGATGTGCTGTTGATGCTCGGCACCGACTTTCCCTACAAGCAGTTCCTGCCGACCGGCGCGCGAATCGCGCAGGTCGACA
TTCGCGCCGAGAATCTCGGTCGCCGTTGCAAGCTCGCGCTTGGCCTGGTCGGCGGTGTCCACGAAACCATCGAGGCCTTG
CTGCCGAAATTGACGACCAAGACCGACCGCGGGCACCTCGATCACAGCGTGGCACGCTACGTCGCATCCCGGCAGGGCCT
CGACGATCTGGCGAAGGGCACGCCCGGCCGCAAGCCGATCCATCCGCAGTATCTCGCCAAGCTGATCAGCGACGGCGCCG
CCGACGATGCGGTGTTCAGCTTCGACGTCGGAACACCGACGATCTGGGCCGCCCGCTATCTGAAAATGAACGGAAGCCGG
CGTCTGGTCGGCTCTCTGGTGCACGGTTCGATGGCCAACGCGCTGCCGCATGCCATCGGCGTGCAGGCCGCCCAGCCGAG
CCGGCAGGTGATCTCGCTGTCGGGGGATGGTGGCTTCACCATGCTGATGGGAGACCTCATCACGCTCACGCAGATGAAAT
TGCCGGTCAAGGTCGTCATTTTCAACAATGGCGTACTCGGCTTCGTGGCGCTCGAGATGAAGGCGGCGGGATTTGTCGAG
TTGGGCACCGATCTACAGAATCCCGATTTCGCCGCCATGGCGCGTGCGATGGGCATCCATGGCGTGCGGGTTGAGGATCC
CGGCGATCTGCCGGCGGCGGTGGCCGACGTGCTGGCTCATGATGGCCCAGCCGTGCTCGACGTCGTCACCGCGACCCAGG
AGCTGTCGATGCCGCCCACCATCGGCGCCGAACAGGTCAAGGGCTTCAGTCTCTGGCTGCTCCGCGCGGTGATGAGCGGC
CGCGGTGACGAAGTGATTGATCTCGCGAAGCAGAACCTGCTGCCCCGGTAG

Upstream 100 bases:

>100_bases
AACGGTCCGATCTGCGCGACATATCGATGCCGTCGGTCTGCAGGCGTCGATCGTGTCGAATCGCCGTCACGTCTTGGCCG
GCCAACAAGGAGCAGAGCGG

Downstream 100 bases:

>100_bases
AGCGTTTGGCGCCGGCCGACTTGCGGAACGGCGTCGATCTCATGATGGCGTTCTTGGAGGCTCACCACAGGCACTACCTG
CCGGTAAGGGGACGCGAAGC

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MSNVADRIVETLHQAGVERIFGVVGDSLNGLTEALRQHGSIEWVHVRHEEVAAFAAAGESQITGKLAVCAGSCGPGNLHL
INGLYDAQRTRTPVLAIAAQIPSAEIGGGYFQETHPQNLFRECSVYCELVSDPHQFDYVLENAIRAAVGQRGVAVVVIPG
DVALREASTRGVTPVAGLLPPTPIVTPAEPQLDALAALLNGAGRVTLFCGRGCAGAHAPLMALAEALKSPIVHALGGKEH
VEYDNPYDVGMTGFIGYASGYEAMHACDVLLMLGTDFPYKQFLPTGARIAQVDIRAENLGRRCKLALGLVGGVHETIEAL
LPKLTTKTDRGHLDHSVARYVASRQGLDDLAKGTPGRKPIHPQYLAKLISDGAADDAVFSFDVGTPTIWAARYLKMNGSR
RLVGSLVHGSMANALPHAIGVQAAQPSRQVISLSGDGGFTMLMGDLITLTQMKLPVKVVIFNNGVLGFVALEMKAAGFVE
LGTDLQNPDFAAMARAMGIHGVRVEDPGDLPAAVADVLAHDGPAVLDVVTATQELSMPPTIGAEQVKGFSLWLLRAVMSG
RGDEVIDLAKQNLLPR

Sequences:

>Translated_576_residues
MSNVADRIVETLHQAGVERIFGVVGDSLNGLTEALRQHGSIEWVHVRHEEVAAFAAAGESQITGKLAVCAGSCGPGNLHL
INGLYDAQRTRTPVLAIAAQIPSAEIGGGYFQETHPQNLFRECSVYCELVSDPHQFDYVLENAIRAAVGQRGVAVVVIPG
DVALREASTRGVTPVAGLLPPTPIVTPAEPQLDALAALLNGAGRVTLFCGRGCAGAHAPLMALAEALKSPIVHALGGKEH
VEYDNPYDVGMTGFIGYASGYEAMHACDVLLMLGTDFPYKQFLPTGARIAQVDIRAENLGRRCKLALGLVGGVHETIEAL
LPKLTTKTDRGHLDHSVARYVASRQGLDDLAKGTPGRKPIHPQYLAKLISDGAADDAVFSFDVGTPTIWAARYLKMNGSR
RLVGSLVHGSMANALPHAIGVQAAQPSRQVISLSGDGGFTMLMGDLITLTQMKLPVKVVIFNNGVLGFVALEMKAAGFVE
LGTDLQNPDFAAMARAMGIHGVRVEDPGDLPAAVADVLAHDGPAVLDVVTATQELSMPPTIGAEQVKGFSLWLLRAVMSG
RGDEVIDLAKQNLLPR
>Mature_575_residues
SNVADRIVETLHQAGVERIFGVVGDSLNGLTEALRQHGSIEWVHVRHEEVAAFAAAGESQITGKLAVCAGSCGPGNLHLI
NGLYDAQRTRTPVLAIAAQIPSAEIGGGYFQETHPQNLFRECSVYCELVSDPHQFDYVLENAIRAAVGQRGVAVVVIPGD
VALREASTRGVTPVAGLLPPTPIVTPAEPQLDALAALLNGAGRVTLFCGRGCAGAHAPLMALAEALKSPIVHALGGKEHV
EYDNPYDVGMTGFIGYASGYEAMHACDVLLMLGTDFPYKQFLPTGARIAQVDIRAENLGRRCKLALGLVGGVHETIEALL
PKLTTKTDRGHLDHSVARYVASRQGLDDLAKGTPGRKPIHPQYLAKLISDGAADDAVFSFDVGTPTIWAARYLKMNGSRR
LVGSLVHGSMANALPHAIGVQAAQPSRQVISLSGDGGFTMLMGDLITLTQMKLPVKVVIFNNGVLGFVALEMKAAGFVEL
GTDLQNPDFAAMARAMGIHGVRVEDPGDLPAAVADVLAHDGPAVLDVVTATQELSMPPTIGAEQVKGFSLWLLRAVMSGR
GDEVIDLAKQNLLPR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=598, Percent_Identity=24.7491638795987, Blast_Score=126, Evalue=7e-29,
Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=59.6153846153846, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=28.6231884057971, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081685, Length=528, Percent_Identity=27.8409090909091, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1786717, Length=552, Percent_Identity=25.7246376811594, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788716, Length=471, Percent_Identity=23.1422505307856, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17531299, Length=584, Percent_Identity=25.513698630137, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17531301, Length=584, Percent_Identity=25.513698630137, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=551, Percent_Identity=25.2268602540835, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=21.5447154471545, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922626, Length=481, Percent_Identity=25.7796257796258, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 60865; Mature: 60734

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVADRIVETLHQAGVERIFGVVGDSLNGLTEALRQHGSIEWVHVRHEEVAAFAAAGES
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCC
QITGKLAVCAGSCGPGNLHLINGLYDAQRTRTPVLAIAAQIPSAEIGGGYFQETHPQNLF
CCCCEEEEEECCCCCCCEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHH
RECSVYCELVSDPHQFDYVLENAIRAAVGQRGVAVVVIPGDVALREASTRGVTPVAGLLP
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEHHCCCCCCCCHHHCCC
PTPIVTPAEPQLDALAALLNGAGRVTLFCGRGCAGAHAPLMALAEALKSPIVHALGGKEH
CCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHCCCCCC
VEYDNPYDVGMTGFIGYASGYEAMHACDVLLMLGTDFPYKQFLPTGARIAQVDIRAENLG
CCCCCCCCCCCHHHEECCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEEEEHHHCC
RRCKLALGLVGGVHETIEALLPKLTTKTDRGHLDHSVARYVASRQGLDDLAKGTPGRKPI
CHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCC
HPQYLAKLISDGAADDAVFSFDVGTPTIWAARYLKMNGSRRLVGSLVHGSMANALPHAIG
CHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
VQAAQPSRQVISLSGDGGFTMLMGDLITLTQMKLPVKVVIFNNGVLGFVALEMKAAGFVE
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEHHHCCCEE
LGTDLQNPDFAAMARAMGIHGVRVEDPGDLPAAVADVLAHDGPAVLDVVTATQELSMPPT
ECCCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
IGAEQVKGFSLWLLRAVMSGRGDEVIDLAKQNLLPR
CCHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SNVADRIVETLHQAGVERIFGVVGDSLNGLTEALRQHGSIEWVHVRHEEVAAFAAAGES
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCC
QITGKLAVCAGSCGPGNLHLINGLYDAQRTRTPVLAIAAQIPSAEIGGGYFQETHPQNLF
CCCCEEEEEECCCCCCCEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHH
RECSVYCELVSDPHQFDYVLENAIRAAVGQRGVAVVVIPGDVALREASTRGVTPVAGLLP
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEHHCCCCCCCCHHHCCC
PTPIVTPAEPQLDALAALLNGAGRVTLFCGRGCAGAHAPLMALAEALKSPIVHALGGKEH
CCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHCCCCCC
VEYDNPYDVGMTGFIGYASGYEAMHACDVLLMLGTDFPYKQFLPTGARIAQVDIRAENLG
CCCCCCCCCCCHHHEECCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEEEEHHHCC
RRCKLALGLVGGVHETIEALLPKLTTKTDRGHLDHSVARYVASRQGLDDLAKGTPGRKPI
CHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCC
HPQYLAKLISDGAADDAVFSFDVGTPTIWAARYLKMNGSRRLVGSLVHGSMANALPHAIG
CHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
VQAAQPSRQVISLSGDGGFTMLMGDLITLTQMKLPVKVVIFNNGVLGFVALEMKAAGFVE
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEHHHCCCEE
LGTDLQNPDFAAMARAMGIHGVRVEDPGDLPAAVADVLAHDGPAVLDVVTATQELSMPPT
ECCCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
IGAEQVKGFSLWLLRAVMSGRGDEVIDLAKQNLLPR
CCHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]