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The map label for this gene is nodW [H]

Identifier: 86747308

GI number: 86747308

Start: 197228

End: 197860

Strand: Direct

Name: nodW [H]

Synonym: RPB_0181

Alternate gene names: 86747308

Gene position: 197228-197860 (Clockwise)

Preceding gene: 86747305

Following gene: 86747309

Centisome position: 3.7

GC content: 62.56

Gene sequence:

>633_bases
ATGACAGCATCATCCAGCCCATTGATCGTCGTCGTCGATGACGACGCCGGCATCAGGGACGCACTGGACGGCTTGTTCAG
GTCTATCGGAAACCACGTCATCTCGTTCGAATCGGCACAGCATCTGCTCACGGCCGGCGTTCCGAACGACGCCAATTGCA
TGATCCTCGACGTGCGATTGCCGGGCACCAGCGGGCTCGACCTGCAAGCCATGCTCACCAAGCTCGACATCCGTATTCCG
GTGATTTTCATGACCGGCTACGGCGATGTGCCCATGACTGTCCGTGGTATGAAGGCCGGCGCCGTCGACTTTCTGACCAA
GCCGTTTCGCGACCAGGAAATGCTGGACGCCGTGTCGGCGGCGATCGCGCTGGACCAACGGCGGCGCGAGAGTGACGGCC
GCCGCGAAGACGTCCTGAAGCGCTACGCGGCGCTGACGCCGCGCGAAAAGCAGGTGTTCGACATGGTGTCCGCCGGGTCG
CTCAACAAGAACGTCGCCGCCGATCTCGGTTTGAGCGAGATCACCGTCAAGATTCATCGCGGGCGCGTGATGAAGAAGAT
GGGCGCCCGCTCGTTCGCCGACCTGGTGCGGATGGCCGAATTGATCGCCGCGCCCTCGCGAGATCCGACGTAA

Upstream 100 bases:

>100_bases
CTTGTCTGGCCTTGTGCGAGCCGCCCGCGGGGCCGATTGGCATCAGACGCCGGCTGGAATAAGGTTCTGAAATGACGCAA
GGATCGTCTACAAGGCCCGC

Downstream 100 bases:

>100_bases
CACCATCGTATGACTTATAAGGCCCCGGACTCTGCCGCACTGTTCCGTGACGTCGGATCGCGCTGCATCACCCTTGAGGA
TGGCCGAAATGACCATCGGC

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTASSSPLIVVVDDDAGIRDALDGLFRSIGNHVISFESAQHLLTAGVPNDANCMILDVRLPGTSGLDLQAMLTKLDIRIP
VIFMTGYGDVPMTVRGMKAGAVDFLTKPFRDQEMLDAVSAAIALDQRRRESDGRREDVLKRYAALTPREKQVFDMVSAGS
LNKNVAADLGLSEITVKIHRGRVMKKMGARSFADLVRMAELIAAPSRDPT

Sequences:

>Translated_210_residues
MTASSSPLIVVVDDDAGIRDALDGLFRSIGNHVISFESAQHLLTAGVPNDANCMILDVRLPGTSGLDLQAMLTKLDIRIP
VIFMTGYGDVPMTVRGMKAGAVDFLTKPFRDQEMLDAVSAAIALDQRRRESDGRREDVLKRYAALTPREKQVFDMVSAGS
LNKNVAADLGLSEITVKIHRGRVMKKMGARSFADLVRMAELIAAPSRDPT
>Mature_209_residues
TASSSPLIVVVDDDAGIRDALDGLFRSIGNHVISFESAQHLLTAGVPNDANCMILDVRLPGTSGLDLQAMLTKLDIRIPV
IFMTGYGDVPMTVRGMKAGAVDFLTKPFRDQEMLDAVSAAIALDQRRRESDGRREDVLKRYAALTPREKQVFDMVSAGSL
NKNVAADLGLSEITVKIHRGRVMKKMGARSFADLVRMAELIAAPSRDPT

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=123, Percent_Identity=31.7073170731707, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790102, Length=172, Percent_Identity=30.2325581395349, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788222, Length=206, Percent_Identity=24.2718446601942, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22828; Mature: 22697

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASSSPLIVVVDDDAGIRDALDGLFRSIGNHVISFESAQHLLTAGVPNDANCMILDVRL
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHCCCCCCCCEEEEEEEC
PGTSGLDLQAMLTKLDIRIPVIFMTGYGDVPMTVRGMKAGAVDFLTKPFRDQEMLDAVSA
CCCCCCHHHHHHHHHCCEEEEEEEECCCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHH
AIALDQRRRESDGRREDVLKRYAALTPREKQVFDMVSAGSLNKNVAADLGLSEITVKIHR
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCHHHEEEEEHH
GRVMKKMGARSFADLVRMAELIAAPSRDPT
HHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TASSSPLIVVVDDDAGIRDALDGLFRSIGNHVISFESAQHLLTAGVPNDANCMILDVRL
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHCCCCCCCCEEEEEEEC
PGTSGLDLQAMLTKLDIRIPVIFMTGYGDVPMTVRGMKAGAVDFLTKPFRDQEMLDAVSA
CCCCCCHHHHHHHHHCCEEEEEEEECCCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHH
AIALDQRRRESDGRREDVLKRYAALTPREKQVFDMVSAGSLNKNVAADLGLSEITVKIHR
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCHHHEEEEEHH
GRVMKKMGARSFADLVRMAELIAAPSRDPT
HHHHHHHCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]