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The map label for this gene is cysL [H]

Identifier: 86747305

GI number: 86747305

Start: 193028

End: 193900

Strand: Direct

Name: cysL [H]

Synonym: RPB_0178

Alternate gene names: 86747305

Gene position: 193028-193900 (Clockwise)

Preceding gene: 86747295

Following gene: 86747308

Centisome position: 3.62

GC content: 68.16

Gene sequence:

>873_bases
ATGGCCCTGACATCGGCGCGAATGCGCGCAATCAACGCCGTATTCGAGACCGGCAGCTTTGCCGCGGCCGCGCGGCACAT
CGGCGTGTCGCAATCGGCCGTCGCCCAATCGGTGCGCGAGCTGGAGGCCGAATTCGACGTGGCGCTGTTCGAGCGCCACG
GTTCGAGCCTGATCCCGACCTCGCTGTGCCGCCAGCTCTACGGCGCGACCAGCAAGATGCAGGCGGTGGAAGCCGACGCG
CTGTCGATCCTGCAGCAGCGCGGCGAACTGGCCGGCGGCGAACTGCGCGTCGGCCTCGGCAATTCGATGCCCGGCATGGC
GCTGATCGCCGCGTTCAAGCGGCTGTATCCGAAAGTGCAGGTGGCGATCGAGATCGGGAGCTGGTCGGCGATCGTCGCGG
CCGTGGTCGAACAGCGCGTCGATATCGGCGTGCTGCCCGAGGTGCCGGCGGACAAGCGCTTTCGCCGCGAAATCTGTCTC
GCGCAGCGCGTCGTCGCGCTGTGCCACCCGGACCATCCGCTGCAGGGGCGCGAGCAGGTCGGGATCGCCGAACTGACGCA
ATATCCCCTGGTGTTCCGGTCGCGCGACTCCTCGACGCAACGCACGGTCGACCGCGCCTTCCGCGACAAGGGCTTGCGCC
CGGCGCCGTCACTGATCGTCAACACCCGCGAAGGGATTCTCGAAGCCGTCGCCAATCAGCTCGGCATCGGTTTCATGTGG
GAGCAGGGCTCGAGCCGGACCGACGGCATCGCGCGGATCTCGGTGCCGGAACTCGACGCCGAAGTGCCGGAATGCATCTT
CGCCCTCGCCGGCAAGAAGGGCCGGCTGGTCGAACTGTTCTTCCTCGCCCGCGGCACGCATGGCGTCGCGTAG

Upstream 100 bases:

>100_bases
GTCAACAGCTTTGCTTATGCTCTCCGCGACATTTCCTTTCGAGGCGCGCGACCTTGTCACGAAGACGATGGCCGCCACGG
CCGGGGAGCACCTTGCCGGC

Downstream 100 bases:

>100_bases
TTGGCGTCCTGTCAGTCGCGAAACTTCTTCTTGGGCTTTTTCTTGAAGCCCGGGTTGGGCGCCGACGGATCGGCACGGCG
TGCCTTGCTGACATAGGCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MALTSARMRAINAVFETGSFAAAARHIGVSQSAVAQSVRELEAEFDVALFERHGSSLIPTSLCRQLYGATSKMQAVEADA
LSILQQRGELAGGELRVGLGNSMPGMALIAAFKRLYPKVQVAIEIGSWSAIVAAVVEQRVDIGVLPEVPADKRFRREICL
AQRVVALCHPDHPLQGREQVGIAELTQYPLVFRSRDSSTQRTVDRAFRDKGLRPAPSLIVNTREGILEAVANQLGIGFMW
EQGSSRTDGIARISVPELDAEVPECIFALAGKKGRLVELFFLARGTHGVA

Sequences:

>Translated_290_residues
MALTSARMRAINAVFETGSFAAAARHIGVSQSAVAQSVRELEAEFDVALFERHGSSLIPTSLCRQLYGATSKMQAVEADA
LSILQQRGELAGGELRVGLGNSMPGMALIAAFKRLYPKVQVAIEIGSWSAIVAAVVEQRVDIGVLPEVPADKRFRREICL
AQRVVALCHPDHPLQGREQVGIAELTQYPLVFRSRDSSTQRTVDRAFRDKGLRPAPSLIVNTREGILEAVANQLGIGFMW
EQGSSRTDGIARISVPELDAEVPECIFALAGKKGRLVELFFLARGTHGVA
>Mature_289_residues
ALTSARMRAINAVFETGSFAAAARHIGVSQSAVAQSVRELEAEFDVALFERHGSSLIPTSLCRQLYGATSKMQAVEADAL
SILQQRGELAGGELRVGLGNSMPGMALIAAFKRLYPKVQVAIEIGSWSAIVAAVVEQRVDIGVLPEVPADKRFRREICLA
QRVVALCHPDHPLQGREQVGIAELTQYPLVFRSRDSSTQRTVDRAFRDKGLRPAPSLIVNTREGILEAVANQLGIGFMWE
QGSSRTDGIARISVPELDAEVPECIFALAGKKGRLVELFFLARGTHGVA

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=250, Percent_Identity=27.2, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787879, Length=196, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787601, Length=234, Percent_Identity=26.0683760683761, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI157672245, Length=262, Percent_Identity=27.8625954198473, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787530, Length=258, Percent_Identity=25.1937984496124, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=216, Percent_Identity=24.0740740740741, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31389; Mature: 31257

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTSARMRAINAVFETGSFAAAARHIGVSQSAVAQSVRELEAEFDVALFERHGSSLIPT
CCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
SLCRQLYGATSKMQAVEADALSILQQRGELAGGELRVGLGNSMPGMALIAAFKRLYPKVQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEE
VAIEIGSWSAIVAAVVEQRVDIGVLPEVPADKRFRREICLAQRVVALCHPDHPLQGREQV
EEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHC
GIAELTQYPLVFRSRDSSTQRTVDRAFRDKGLRPAPSLIVNTREGILEAVANQLGIGFMW
CHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHEEECHHHHHHHHHHHHCCEEEE
EQGSSRTDGIARISVPELDAEVPECIFALAGKKGRLVELFFLARGTHGVA
CCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
ALTSARMRAINAVFETGSFAAAARHIGVSQSAVAQSVRELEAEFDVALFERHGSSLIPT
CCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
SLCRQLYGATSKMQAVEADALSILQQRGELAGGELRVGLGNSMPGMALIAAFKRLYPKVQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEE
VAIEIGSWSAIVAAVVEQRVDIGVLPEVPADKRFRREICLAQRVVALCHPDHPLQGREQV
EEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHC
GIAELTQYPLVFRSRDSSTQRTVDRAFRDKGLRPAPSLIVNTREGILEAVANQLGIGFMW
CHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHEEECHHHHHHHHHHHHCCEEEE
EQGSSRTDGIARISVPELDAEVPECIFALAGKKGRLVELFFLARGTHGVA
CCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]