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The map label for this gene is phaD [H]

Identifier: 86747277

GI number: 86747277

Start: 163912

End: 165447

Strand: Direct

Name: phaD [H]

Synonym: RPB_0150

Alternate gene names: 86747277

Gene position: 163912-165447 (Clockwise)

Preceding gene: 86747276

Following gene: 86747278

Centisome position: 3.07

GC content: 69.6

Gene sequence:

>1536_bases
ATGAGTCACCTGATCGTCGCCCCGATCATCCTGCCCGCCGTCGTCGCCGCCTTCCTGGTGCTGGTGCTGCGGCACGATCT
GACCAGCCAGCGCATCGTCTCGATGGCGTCGACGCTCGTGCTGCTCGCGATCACGATCTGGCTCTATGTGTCGGCGGCGG
AGGGCGCGCCGACGCCCTATCTGCTCGGCGAATGGCGCGCGCCGTTCGGCATCGTGCTGGTGCTCGACCGCCTCGCGGCG
ACGATGCTGCTGCTGACGGCGCTGCTGGCCGTCGCGGTGCTGATCTATGCGATCGCCGGCTGGGACCATCGCGGCCAGCA
TTTCCATCCGCTGTTCCAGTTTCAGCTGATGGGCCTCAACGGCGCTTTCCTGACCGGCGACGTGTTCAATTTGTTCGTGT
TCTTCGAGGTCATGCTGATCGCCTCCTACGGCCTGATGCTGCATGGCGGCGGCGCGCGGCGGCTGCGCACGGGCTTCCAC
TATATCGCGATCAATCTGGTCGGCTCGACGCTGTTCCTGTTCGCCGTCGGCCTGATCTACGCGGTGACCGGCACGCTCAA
CATGGCGGACCTCGCCGCCCGGGTGGCGCAGATCGCGCCCGGCGACGAAGCCATCCTGCGCGTCGGGGCGCTGCTGCTGC
TGCTGGTGTTCGCGCTCAAGGCCGCGTTGGTGCCGCTGCAATGGTGGCTGCCCTCGGCCTATGCGGCGGCGCCGGGGCCG
GTGGCGGCGCTGTTCATGATGATGACCAAGGTCGGTGCCTATTCGATCGTGCGGGTCTACACTGTAGTGTTCGGCGCCGA
CGCCGGCGCGTTGGCGGACATCGCCGCGCCCTGGGTGGTGCCCGCGGCGCTGGCGACGCTGGCGCTCGGCGCGGTCGGCG
TGCTGGCGAGCCCGACCTTGCTGGGGATGGTCTGCTTCTCGATCGTCTGGTCGATGGGCTCGCTGCTGCTGGCGCTCGGC
CTGTTCGACCCGGTCGGCCTGTCCGCCGGGCTGTATTATCTGCTGCACTCAACGCTGAGCGGCGCCGCGCTGTTCCTGCT
GGCCGATCTGGTCGCCGCGCAGCGCGGTCGCCTCGCCGACCGGCTGGTGCCGGCGCCGCGGCTGCCCCATGCCGATCTGC
TCGCCGGGCTGTTCTTCGTCGCGGCGATCGCCATGACCGGGATGCCGCCGCTGTCGGGTTTTCTCGGCAAGCTGTTGATC
CTCGACGCGACCTGGCGCAGCCCGCTCGCGGTGACGAGCTGGGTGATCCTGCTCGGAAGCAGCCTTCTGGTGATCGTCGG
CTTCGCCCGCGCCGGCAGCACGCTGTTCTGGAAGATCGCCGACCGGCCGGCCGAGCCGGCCGAACCGGCGCGGTCGATCG
CGCTGCCGGTCACGGTGATCGCCGGTCTGCTCGGCGCCACCGCGCTGCTGTCGGTGTTCGCCGGCCCGATCAGCCGCGAA
CTGGCGGCGACGGCGCAACAGACGCTCGACATCAAGGCCTATGTCCGCGCGGTGAACCCCGCCGACCTGGCACTGTCGCG
GGGGGCGCCGCAATGA

Upstream 100 bases:

>100_bases
TGGTGGTGCTGGCGCTGCGCGGCTTCCTCGAGACCGGCACCGAGCGGGTCGAGAAGGGCGAACCGCAGCGCGACGCGCGC
AAGCGCAAGGCGGCGCAGCC

Downstream 100 bases:

>100_bases
CCAGGATGTTTCCGCATCCGATCCTCAGCCTGGTGATCGCCGGCGTGTTCGTCGGCCTGATCAACACGCTGTCGCTCGGG
AGCGTGGTGCTGGGCCTGCT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSHLIVAPIILPAVVAAFLVLVLRHDLTSQRIVSMASTLVLLAITIWLYVSAAEGAPTPYLLGEWRAPFGIVLVLDRLAA
TMLLLTALLAVAVLIYAIAGWDHRGQHFHPLFQFQLMGLNGAFLTGDVFNLFVFFEVMLIASYGLMLHGGGARRLRTGFH
YIAINLVGSTLFLFAVGLIYAVTGTLNMADLAARVAQIAPGDEAILRVGALLLLLVFALKAALVPLQWWLPSAYAAAPGP
VAALFMMMTKVGAYSIVRVYTVVFGADAGALADIAAPWVVPAALATLALGAVGVLASPTLLGMVCFSIVWSMGSLLLALG
LFDPVGLSAGLYYLLHSTLSGAALFLLADLVAAQRGRLADRLVPAPRLPHADLLAGLFFVAAIAMTGMPPLSGFLGKLLI
LDATWRSPLAVTSWVILLGSSLLVIVGFARAGSTLFWKIADRPAEPAEPARSIALPVTVIAGLLGATALLSVFAGPISRE
LAATAQQTLDIKAYVRAVNPADLALSRGAPQ

Sequences:

>Translated_511_residues
MSHLIVAPIILPAVVAAFLVLVLRHDLTSQRIVSMASTLVLLAITIWLYVSAAEGAPTPYLLGEWRAPFGIVLVLDRLAA
TMLLLTALLAVAVLIYAIAGWDHRGQHFHPLFQFQLMGLNGAFLTGDVFNLFVFFEVMLIASYGLMLHGGGARRLRTGFH
YIAINLVGSTLFLFAVGLIYAVTGTLNMADLAARVAQIAPGDEAILRVGALLLLLVFALKAALVPLQWWLPSAYAAAPGP
VAALFMMMTKVGAYSIVRVYTVVFGADAGALADIAAPWVVPAALATLALGAVGVLASPTLLGMVCFSIVWSMGSLLLALG
LFDPVGLSAGLYYLLHSTLSGAALFLLADLVAAQRGRLADRLVPAPRLPHADLLAGLFFVAAIAMTGMPPLSGFLGKLLI
LDATWRSPLAVTSWVILLGSSLLVIVGFARAGSTLFWKIADRPAEPAEPARSIALPVTVIAGLLGATALLSVFAGPISRE
LAATAQQTLDIKAYVRAVNPADLALSRGAPQ
>Mature_510_residues
SHLIVAPIILPAVVAAFLVLVLRHDLTSQRIVSMASTLVLLAITIWLYVSAAEGAPTPYLLGEWRAPFGIVLVLDRLAAT
MLLLTALLAVAVLIYAIAGWDHRGQHFHPLFQFQLMGLNGAFLTGDVFNLFVFFEVMLIASYGLMLHGGGARRLRTGFHY
IAINLVGSTLFLFAVGLIYAVTGTLNMADLAARVAQIAPGDEAILRVGALLLLLVFALKAALVPLQWWLPSAYAAAPGPV
AALFMMMTKVGAYSIVRVYTVVFGADAGALADIAAPWVVPAALATLALGAVGVLASPTLLGMVCFSIVWSMGSLLLALGL
FDPVGLSAGLYYLLHSTLSGAALFLLADLVAAQRGRLADRLVPAPRLPHADLLAGLFFVAAIAMTGMPPLSGFLGKLLIL
DATWRSPLAVTSWVILLGSSLLVIVGFARAGSTLFWKIADRPAEPAEPARSIALPVTVIAGLLGATALLSVFAGPISREL
AATAQQTLDIKAYVRAVNPADLALSRGAPQ

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788829, Length=394, Percent_Identity=26.6497461928934, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788831, Length=399, Percent_Identity=24.5614035087719, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788613, Length=396, Percent_Identity=26.7676767676768, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788827, Length=298, Percent_Identity=28.1879194630872, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693160, Length=376, Percent_Identity=24.2021276595745, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53708; Mature: 53577

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLIVAPIILPAVVAAFLVLVLRHDLTSQRIVSMASTLVLLAITIWLYVSAAEGAPTPY
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LLGEWRAPFGIVLVLDRLAATMLLLTALLAVAVLIYAIAGWDHRGQHFHPLFQFQLMGLN
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEECCC
GAFLTGDVFNLFVFFEVMLIASYGLMLHGGGARRLRTGFHYIAINLVGSTLFLFAVGLIY
CCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AVTGTLNMADLAARVAQIAPGDEAILRVGALLLLLVFALKAALVPLQWWLPSAYAAAPGP
HHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VAALFMMMTKVGAYSIVRVYTVVFGADAGALADIAAPWVVPAALATLALGAVGVLASPTL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGMVCFSIVWSMGSLLLALGLFDPVGLSAGLYYLLHSTLSGAALFLLADLVAAQRGRLAD
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RLVPAPRLPHADLLAGLFFVAAIAMTGMPPLSGFLGKLLILDATWRSPLAVTSWVILLGS
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHCC
SLLVIVGFARAGSTLFWKIADRPAEPAEPARSIALPVTVIAGLLGATALLSVFAGPISRE
HHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LAATAQQTLDIKAYVRAVNPADLALSRGAPQ
HHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
>Mature Secondary Structure 
SHLIVAPIILPAVVAAFLVLVLRHDLTSQRIVSMASTLVLLAITIWLYVSAAEGAPTPY
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LLGEWRAPFGIVLVLDRLAATMLLLTALLAVAVLIYAIAGWDHRGQHFHPLFQFQLMGLN
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEECCC
GAFLTGDVFNLFVFFEVMLIASYGLMLHGGGARRLRTGFHYIAINLVGSTLFLFAVGLIY
CCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AVTGTLNMADLAARVAQIAPGDEAILRVGALLLLLVFALKAALVPLQWWLPSAYAAAPGP
HHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VAALFMMMTKVGAYSIVRVYTVVFGADAGALADIAAPWVVPAALATLALGAVGVLASPTL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGMVCFSIVWSMGSLLLALGLFDPVGLSAGLYYLLHSTLSGAALFLLADLVAAQRGRLAD
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RLVPAPRLPHADLLAGLFFVAAIAMTGMPPLSGFLGKLLILDATWRSPLAVTSWVILLGS
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHCC
SLLVIVGFARAGSTLFWKIADRPAEPAEPARSIALPVTVIAGLLGATALLSVFAGPISRE
HHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LAATAQQTLDIKAYVRAVNPADLALSRGAPQ
HHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]