Click here to switch to the map view.

The map label for this gene is puuB [H]

Identifier: 86747266

GI number: 86747266

Start: 151457

End: 152851

Strand: Direct

Name: puuB [H]

Synonym: RPB_0139

Alternate gene names: 86747266

Gene position: 151457-152851 (Clockwise)

Preceding gene: 86747265

Following gene: 86747268

Centisome position: 2.84

GC content: 67.74

Gene sequence:

>1395_bases
ATGAACCGGCCGCTGAAGACGCCGCCGGTCGCGGCCTACGATCCGCATTACGATCCGCTGGTCAGCGACGGCCCGGGCCA
CAATCGCGACTACGCGCCAACCTACTGGCACGCCACCGCCGGTCCGCTGCCCGCCGACGACGGCCCGGTGACATCCGACA
TCGACGCCGACGTCGCGATCGTCGGGTCCGGCTACACCGGACTCGCCTGCGCGATCTATCTGGCTCGCGAACATGGTATC
AAGCCCATCGTGCTGGAGGCCAATCGCGTCGCCTGGGGCTGCAGCACCCGCAATGGCGGCCAGGGCCAGAACGCCGCCGG
CCGACTGACACGGTCGCAATGGATCGCGCGCTGGGGCATCGACGTCGCAAAGCAGCTTCACGCCGAAATCCGCGACGGCT
TCGACACCTTCGAGGAGTTGATCCGGCAAAGCCCGATCGATTGCGAGCCGCAGCGCGGCGGGCATCTCTACATTGCGCAC
CGCCAGCGCAACCTCGACAAGATCGCCGCCGAAGCTAAGGTGCTGCGCGACGTGTTCGGCGAAAAGACCAGCGTGGTGAG
CGCCGAGCAACTGCGGAGCGACTATCTCAATGAAGCCGAAGCGGTCGGCGCCGTGCTGGAGCCGCTCGGCACCGGCGTGC
ACCCCGCCAAGCTCGCTTTCGGCTATCAGCAGATGGCCCGCGATCTCGGCGTCAGGATTCATCCGGCGAGCCCCGTCGCC
GAAATCCAGTCCCGCGACGGCGCGCTGTATCTGCGCACGCCGGGCGGCACGGTGCGGGCGCGCGCGGTCGGCATCGCCAC
CGGCGCCTACACGGCGCCCGGCCTGACCCCGGCGCTGCGCGGCCGCTGCATGCCGATCCTGTCGAACTCGATCGTGACCC
GGCCGCTGACCGCGGCCGAACTCGACGCCACCGGCTTCAGGACCAAGCTGGTGCTGACCGACACAAGGACGCTGCGCTAC
TACTACCGCCTGCTGCCCGACAACCGCATCCAGATCGGCAGCCGCTCCTCGATCACCGGCGCCGACGCCGATCATCCGAA
GCATCTCCAGCTCCTGATCGACGGGCTGCATCGCAAATTTCCGGCGCTGCAGGGCATCGAGATCGACTATTCGTGGTGGG
GCTGGGTCGACGTCAGCCACGACATGATGCCGCGCATCTTCCAGCCCGATCAGCAGACCAAGATGTTCTACGCGCTCGGC
TATGGCGGCAACGGCGTGTCCTATTCGGCCCAGGCCGGCCGCCGCATGGCGCAGATGATCGCCGGCCAGCACTTCAAGGG
CCAGGACCTGCCGATCTTCACCTCGCCGCTGCCGACCCACACTTTCGAGCCGTTCCGTCGGCTCGGCCAGCGCATGCTGT
ATCGCTGGTACCACATGCGCGACGAGGCGGCGTAG

Upstream 100 bases:

>100_bases
CCGAGGGCGTCGACCTGTTCGGATTCCGCGGCGAGCTGATCTGCAGCAAGAGCGCATTCCGCAAGGACCGGCCGGCGCTG
CCCGCCCAGGGAGCCGCGTG

Downstream 100 bases:

>100_bases
CCGTTCGCTTCCTCGCTGAAAGTCGAGTTTCGCGCCGTCCTAACAACCCGTCTTGGCCGGCCTTGTGCCGGCCATCCACG
AACTGGGCTCACGCCGCAAG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNRPLKTPPVAAYDPHYDPLVSDGPGHNRDYAPTYWHATAGPLPADDGPVTSDIDADVAIVGSGYTGLACAIYLAREHGI
KPIVLEANRVAWGCSTRNGGQGQNAAGRLTRSQWIARWGIDVAKQLHAEIRDGFDTFEELIRQSPIDCEPQRGGHLYIAH
RQRNLDKIAAEAKVLRDVFGEKTSVVSAEQLRSDYLNEAEAVGAVLEPLGTGVHPAKLAFGYQQMARDLGVRIHPASPVA
EIQSRDGALYLRTPGGTVRARAVGIATGAYTAPGLTPALRGRCMPILSNSIVTRPLTAAELDATGFRTKLVLTDTRTLRY
YYRLLPDNRIQIGSRSSITGADADHPKHLQLLIDGLHRKFPALQGIEIDYSWWGWVDVSHDMMPRIFQPDQQTKMFYALG
YGGNGVSYSAQAGRRMAQMIAGQHFKGQDLPIFTSPLPTHTFEPFRRLGQRMLYRWYHMRDEAA

Sequences:

>Translated_464_residues
MNRPLKTPPVAAYDPHYDPLVSDGPGHNRDYAPTYWHATAGPLPADDGPVTSDIDADVAIVGSGYTGLACAIYLAREHGI
KPIVLEANRVAWGCSTRNGGQGQNAAGRLTRSQWIARWGIDVAKQLHAEIRDGFDTFEELIRQSPIDCEPQRGGHLYIAH
RQRNLDKIAAEAKVLRDVFGEKTSVVSAEQLRSDYLNEAEAVGAVLEPLGTGVHPAKLAFGYQQMARDLGVRIHPASPVA
EIQSRDGALYLRTPGGTVRARAVGIATGAYTAPGLTPALRGRCMPILSNSIVTRPLTAAELDATGFRTKLVLTDTRTLRY
YYRLLPDNRIQIGSRSSITGADADHPKHLQLLIDGLHRKFPALQGIEIDYSWWGWVDVSHDMMPRIFQPDQQTKMFYALG
YGGNGVSYSAQAGRRMAQMIAGQHFKGQDLPIFTSPLPTHTFEPFRRLGQRMLYRWYHMRDEAA
>Mature_464_residues
MNRPLKTPPVAAYDPHYDPLVSDGPGHNRDYAPTYWHATAGPLPADDGPVTSDIDADVAIVGSGYTGLACAIYLAREHGI
KPIVLEANRVAWGCSTRNGGQGQNAAGRLTRSQWIARWGIDVAKQLHAEIRDGFDTFEELIRQSPIDCEPQRGGHLYIAH
RQRNLDKIAAEAKVLRDVFGEKTSVVSAEQLRSDYLNEAEAVGAVLEPLGTGVHPAKLAFGYQQMARDLGVRIHPASPVA
EIQSRDGALYLRTPGGTVRARAVGIATGAYTAPGLTPALRGRCMPILSNSIVTRPLTAAELDATGFRTKLVLTDTRTLRY
YYRLLPDNRIQIGSRSSITGADADHPKHLQLLIDGLHRKFPALQGIEIDYSWWGWVDVSHDMMPRIFQPDQQTKMFYALG
YGGNGVSYSAQAGRRMAQMIAGQHFKGQDLPIFTSPLPTHTFEPFRRLGQRMLYRWYHMRDEAA

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=439, Percent_Identity=30.5239179954442, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51246; Mature: 51246

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPLKTPPVAAYDPHYDPLVSDGPGHNRDYAPTYWHATAGPLPADDGPVTSDIDADVAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEE
VGSGYTGLACAIYLAREHGIKPIVLEANRVAWGCSTRNGGQGQNAAGRLTRSQWIARWGI
EECCCHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
DVAKQLHAEIRDGFDTFEELIRQSPIDCEPQRGGHLYIAHRQRNLDKIAAEAKVLRDVFG
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
EKTSVVSAEQLRSDYLNEAEAVGAVLEPLGTGVHPAKLAFGYQQMARDLGVRIHPASPVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCCHH
EIQSRDGALYLRTPGGTVRARAVGIATGAYTAPGLTPALRGRCMPILSNSIVTRPLTAAE
HHHCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCHHHCCCCHHHHCCCHHCCCCCHHH
LDATGFRTKLVLTDTRTLRYYYRLLPDNRIQIGSRSSITGADADHPKHLQLLIDGLHRKF
CCCCCCEEEEEEECCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
PALQGIEIDYSWWGWVDVSHDMMPRIFQPDQQTKMFYALGYGGNGVSYSAQAGRRMAQMI
CCCCCEEEECCEEEEEECCHHHCHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
AGQHFKGQDLPIFTSPLPTHTFEPFRRLGQRMLYRWYHMRDEAA
HCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRPLKTPPVAAYDPHYDPLVSDGPGHNRDYAPTYWHATAGPLPADDGPVTSDIDADVAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEE
VGSGYTGLACAIYLAREHGIKPIVLEANRVAWGCSTRNGGQGQNAAGRLTRSQWIARWGI
EECCCHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
DVAKQLHAEIRDGFDTFEELIRQSPIDCEPQRGGHLYIAHRQRNLDKIAAEAKVLRDVFG
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
EKTSVVSAEQLRSDYLNEAEAVGAVLEPLGTGVHPAKLAFGYQQMARDLGVRIHPASPVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCCHH
EIQSRDGALYLRTPGGTVRARAVGIATGAYTAPGLTPALRGRCMPILSNSIVTRPLTAAE
HHHCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCHHHCCCCHHHHCCCHHCCCCCHHH
LDATGFRTKLVLTDTRTLRYYYRLLPDNRIQIGSRSSITGADADHPKHLQLLIDGLHRKF
CCCCCCEEEEEEECCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
PALQGIEIDYSWWGWVDVSHDMMPRIFQPDQQTKMFYALGYGGNGVSYSAQAGRRMAQMI
CCCCCEEEECCEEEEEECCHHHCHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
AGQHFKGQDLPIFTSPLPTHTFEPFRRLGQRMLYRWYHMRDEAA
HCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]