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The map label for this gene is livK [C]

Identifier: 86747253

GI number: 86747253

Start: 136074

End: 137282

Strand: Direct

Name: livK [C]

Synonym: RPB_0126

Alternate gene names: 86747253

Gene position: 136074-137282 (Clockwise)

Preceding gene: 86747251

Following gene: 86747254

Centisome position: 2.55

GC content: 65.92

Gene sequence:

>1209_bases
ATGGCTGGGCGCTTCGCGGTGAAACTGGTCTCGGTCGCGGTGGCATTGATGGCGAGCGGTTGGCGGGCCGACGCCGCCGA
TCAACCACCGATCCGGATCGGCGACGTCTCCAGCTATTCGGCTCTGCCGATCGGCGCCCGTGGCTATCGGCAGGGCTGGG
AGCTCGCGCGCGACGAGATCAACGGCAAAGGCGGCGTGCTGGGCCGGCAGCTCCAGATCATCTCCCGCGACGACGCCGGC
AAGCCCGACGCGGCCATCACGCAGGCCGCGCAACTGGTCGACGCGGAGAACGTGGATCTGCTGACCGGGACGATCCTCTC
CAATGTCGGCCTCGCCGTCTCCGATTTCGCCAAACGGCGGAAGATCTTCTTCCTGGCCTCGCAGCCCCTGACCGACGCGC
TGATCTGGGACAAGCGCAGCCGCTACACTTACCGGCTGCGGCCTTCGACCTACACCCAGACCACCATCCTGGCCCAGGAA
GCCGCCAAGCTGCCGGCGCGGCGCTGGGCCACGATCGCACCCAACTACGAGTTCGGGCAGGCGGCCGTCTCCAACTTCAA
GCAGGAATTGCGGCGACTCCGACCTGATGTCGAGTTCATTTCCGAGCAATGGCCGCCGCTCGGCAAGATCGATGCGGGCT
CGGTCGTGCAGGCGATGGCCGCCGATAACCCGGAGGCGATCTTCAACGTCACCTTCGGGTCCGATCTCGCCCGGCTGGTG
CGGGAGGGAACCACGCGCAAGACGTTTGCGAACCGCACCGTCGTCAGCCTCCTGACCGGCGAGCCGGAATATCTGGCGCC
GCTGAAGGACGAGGCGCCCGAGGGATGGATCGTGACCGGCTATCCGTGGAACGAGATCGCGACGCCAGAGCACCGCGCCT
TCGTCGAGGCCTATCGGGCGCGCTTCAAGGATGCGCCGAACATCGGTGCGCTGATCGGCTACATCAACACGCTGGTGCTC
GCCAAAGCGATCGAAACCGCCGGCTCGACCGAGACCGAAGCGCTTCTCGCGGCGATGGAGCACTTGAAAATCGACACGCC
GTGCGGGCCGATCTCGTTCCGTGCCGTCGACCATCAGTCCACGCTGGGCATCTACATCGGCAAGCTGGCGGTGAGGGATG
GCCAGGGGGTGATGACCGACTGGCGTTATGTCGACGGCGCCACTCACCAGCCGTCGGACGCCGACGTTCTGGCCAAAATC
AAAGAGTAG

Upstream 100 bases:

>100_bases
CAAGCCTTGCCACTTCTTGGGCGTTGACAATACCTCCAGAACGCATTCTGTTATGTAGTAAGTACTACATTCAATTGCAA
CGGGGTCATGGAGGTTCGGG

Downstream 100 bases:

>100_bases
CGCCGGCCATCGCCCTCATCACAACGCCACACGCCGTGAAAGACTCCGGAATGAAACTGATCGCCCCCCGCAACAACTGG
TATCCGCTGACATGGTCACG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: Transporter Signal Peptide Protein; Amino Acid ABC Transporter Periplasmic Protein; ABC Transporter Periplasmic Protein; ABC Transporter Subunit; Amino Acid ABC Transporter; Histidine Kinase; ABC-Transporter; Branched Amino Acid ABC TRANSPORTER Periplasmic Component; Tat Pathway Signal Sequence Domain-Containing Protein; Aliphatic Amidase Expression-Regulating Protein; ABC-Type Branched-Chain Amino Acid Transport Systems; Branched-Chain Amino Acid ABC Transporter Permease Protein; Signal Protein In Twin-Arginine Translocation Pathway; Exported Protein

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MAGRFAVKLVSVAVALMASGWRADAADQPPIRIGDVSSYSALPIGARGYRQGWELARDEINGKGGVLGRQLQIISRDDAG
KPDAAITQAAQLVDAENVDLLTGTILSNVGLAVSDFAKRRKIFFLASQPLTDALIWDKRSRYTYRLRPSTYTQTTILAQE
AAKLPARRWATIAPNYEFGQAAVSNFKQELRRLRPDVEFISEQWPPLGKIDAGSVVQAMAADNPEAIFNVTFGSDLARLV
REGTTRKTFANRTVVSLLTGEPEYLAPLKDEAPEGWIVTGYPWNEIATPEHRAFVEAYRARFKDAPNIGALIGYINTLVL
AKAIETAGSTETEALLAAMEHLKIDTPCGPISFRAVDHQSTLGIYIGKLAVRDGQGVMTDWRYVDGATHQPSDADVLAKI
KE

Sequences:

>Translated_402_residues
MAGRFAVKLVSVAVALMASGWRADAADQPPIRIGDVSSYSALPIGARGYRQGWELARDEINGKGGVLGRQLQIISRDDAG
KPDAAITQAAQLVDAENVDLLTGTILSNVGLAVSDFAKRRKIFFLASQPLTDALIWDKRSRYTYRLRPSTYTQTTILAQE
AAKLPARRWATIAPNYEFGQAAVSNFKQELRRLRPDVEFISEQWPPLGKIDAGSVVQAMAADNPEAIFNVTFGSDLARLV
REGTTRKTFANRTVVSLLTGEPEYLAPLKDEAPEGWIVTGYPWNEIATPEHRAFVEAYRARFKDAPNIGALIGYINTLVL
AKAIETAGSTETEALLAAMEHLKIDTPCGPISFRAVDHQSTLGIYIGKLAVRDGQGVMTDWRYVDGATHQPSDADVLAKI
KE
>Mature_401_residues
AGRFAVKLVSVAVALMASGWRADAADQPPIRIGDVSSYSALPIGARGYRQGWELARDEINGKGGVLGRQLQIISRDDAGK
PDAAITQAAQLVDAENVDLLTGTILSNVGLAVSDFAKRRKIFFLASQPLTDALIWDKRSRYTYRLRPSTYTQTTILAQEA
AKLPARRWATIAPNYEFGQAAVSNFKQELRRLRPDVEFISEQWPPLGKIDAGSVVQAMAADNPEAIFNVTFGSDLARLVR
EGTTRKTFANRTVVSLLTGEPEYLAPLKDEAPEGWIVTGYPWNEIATPEHRAFVEAYRARFKDAPNIGALIGYINTLVLA
KAIETAGSTETEALLAAMEHLKIDTPCGPISFRAVDHQSTLGIYIGKLAVRDGQGVMTDWRYVDGATHQPSDADVLAKIK
E

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 43929; Mature: 43798

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRFAVKLVSVAVALMASGWRADAADQPPIRIGDVSSYSALPIGARGYRQGWELARDEI
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
NGKGGVLGRQLQIISRDDAGKPDAAITQAAQLVDAENVDLLTGTILSNVGLAVSDFAKRR
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHCC
KIFFLASQPLTDALIWDKRSRYTYRLRPSTYTQTTILAQEAAKLPARRWATIAPNYEFGQ
EEEEEECCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHCEEECCCCCHHH
AAVSNFKQELRRLRPDVEFISEQWPPLGKIDAGSVVQAMAADNPEAIFNVTFGSDLARLV
HHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHH
REGTTRKTFANRTVVSLLTGEPEYLAPLKDEAPEGWIVTGYPWNEIATPEHRAFVEAYRA
HCCCCHHHHHCCCEEEEECCCCHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
RFKDAPNIGALIGYINTLVLAKAIETAGSTETEALLAAMEHLKIDTPCGPISFRAVDHQS
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
TLGIYIGKLAVRDGQGVMTDWRYVDGATHQPSDADVLAKIKE
CCEEEEEEEEEECCCCCEECCEEECCCCCCCCCCHHHHEECC
>Mature Secondary Structure 
AGRFAVKLVSVAVALMASGWRADAADQPPIRIGDVSSYSALPIGARGYRQGWELARDEI
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
NGKGGVLGRQLQIISRDDAGKPDAAITQAAQLVDAENVDLLTGTILSNVGLAVSDFAKRR
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHCC
KIFFLASQPLTDALIWDKRSRYTYRLRPSTYTQTTILAQEAAKLPARRWATIAPNYEFGQ
EEEEEECCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHCEEECCCCCHHH
AAVSNFKQELRRLRPDVEFISEQWPPLGKIDAGSVVQAMAADNPEAIFNVTFGSDLARLV
HHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHH
REGTTRKTFANRTVVSLLTGEPEYLAPLKDEAPEGWIVTGYPWNEIATPEHRAFVEAYRA
HCCCCHHHHHCCCEEEEECCCCHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
RFKDAPNIGALIGYINTLVLAKAIETAGSTETEALLAAMEHLKIDTPCGPISFRAVDHQS
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
TLGIYIGKLAVRDGQGVMTDWRYVDGATHQPSDADVLAKIKE
CCEEEEEEEEEECCCCCEECCEEECCCCCCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA