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The map label for this gene is dapE [H]

Identifier: 86747234

GI number: 86747234

Start: 117568

End: 119049

Strand: Direct

Name: dapE [H]

Synonym: RPB_0107

Alternate gene names: 86747234

Gene position: 117568-119049 (Clockwise)

Preceding gene: 86747233

Following gene: 86747245

Centisome position: 2.21

GC content: 67.34

Gene sequence:

>1482_bases
ATGCGCCGGCTGCTGAGATGGATCCGCAATATCGTGGTGGTCGCGGTGATCGCGATCGCCGGCCTGGCCGTAGCGCTGAC
CTACAATACGTTTCGTCAGCCCTCGCGGCAGATCGACGTCGCTGCCGTTGCGCCGGTGGCGGTGGACGAGGACGGCGCCG
CGCGGCGGCTCGCCGAAGCGATCCGCTTCCCGACCATCTCCAACTTCCTCAACCCGGAGCAGGACGCCGAGGCGCTGCGC
GGCCTGCAGGCGCATATCGTGGCGAGCTTCCCGGCGTTTCATGCCGCCGCGACCCGCGAAGTCGTCAACGGCAAGAGCCT
GCTCTACACGTGGCAGGGCACCGACCCCCAGGCCCGGCCGATCGCGCTGCTGGCCCATCAGGACGTGGTGCCGATCGCGC
CGAAGACCGAACAGGACTGGCAGCACAAGCCATTCGATGGCGTGATCGCCGACGGCTTCGTCTGGGGCCGCGGCTCGTGG
GACGACAAGGGCAATCTCTACGCCATGCTGGAAGCCGTCGAGGCGATGGCGAAGCAGGGCTTCCGGCCGAAACGCACGAT
CTATTTCGCCTTCGGCCACGACGAAGAAGTCTCCGGCCTGCGCGGCGCCAGGCAGATCGCCGACCTGCTCGCCGCGCGAA
AAGTCCGGCTCGACTTCGTGCTCGACGAGGGCCTGCTGATCACCGACGGCATCATGAAGGGCCTCGACCGGCCGGCAGCG
CTGATCGGCGTCTCGGAAAAGGGCTACGCCACGCTGGTGCTGACCGCACGCGGCACGCCAGGCCATTCCTCGATGCCGCC
GCGCGACACCGCGATCGGAATGCTCGCGGCGGCGCTGACGCATCTCGAAGACAACCGCCTGCCGATGCGGGTGCGCGGCT
CGGTCGCCGACATGTTCGACACGCTGGCGCCGGAAATGCGCGGCTTCAATCGCGTCGTGCTGTCGAACCTGTGGCTGTTC
AAGCCGCTGTTGCTACGCGAATTCGCCAAGAGCGGCACCACCGAGGCGATGGTCCGCACCACCACCGCGCTGACCGTGTT
CAACGCCGGCGACAAGGACAACGTGCTGCCCGGCATTGCGGAGGCCAGCGTCAATTTCCGGCTGCTGCCGGGCGACACCC
AGGCCGGCATCACCGACCATGTCCGCAAGACCGTCGCCAACGACCGGATCGCGATCGCCGGATCGGAGGGCAATTTCGAT
CCGCCGCCGGTGACCGGCACCGCGAGCGCTTCCTACGAGGCCCTCAACCGCACCATCCGCGAAATCTTTCCGGACGTGGT
TGTGGCGCCCGGCCTGATGATCGCCGCCACCGACTCGCGGCACTATGCCGGCGTCGCCGACAATATCTTCCGGTTCTCGC
CGGTCCGCGCCACGTCCGAGGACCTCAAGCGCTTCCACGGCACCAACGAGCGCATCAGCATCGCCAACTACGCCGACATG
ATCCGGTTCTATGTGCGGCTGATCCAGAACACCGCCGGCTGA

Upstream 100 bases:

>100_bases
GCGCCGAGGATGCGCACAAGCTGATCGTCGAAGGCATCGAGCGGGCCAAGGCCGCAAAGAAATAAGCCGCGGCTGGCCCG
CGGCCTCAAAGGGGACTGCG

Downstream 100 bases:

>100_bases
GGCGGTCTGGTTCCCGCAGAAACCTCTCGACGAATACGCCGTCATCCTGAGGTGTCGCCGCAAAGCGGCGGCCTCGAAGG
ATGGGGCACGCGTACCGGCA

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRRLLRWIRNIVVVAVIAIAGLAVALTYNTFRQPSRQIDVAAVAPVAVDEDGAARRLAEAIRFPTISNFLNPEQDAEALR
GLQAHIVASFPAFHAAATREVVNGKSLLYTWQGTDPQARPIALLAHQDVVPIAPKTEQDWQHKPFDGVIADGFVWGRGSW
DDKGNLYAMLEAVEAMAKQGFRPKRTIYFAFGHDEEVSGLRGARQIADLLAARKVRLDFVLDEGLLITDGIMKGLDRPAA
LIGVSEKGYATLVLTARGTPGHSSMPPRDTAIGMLAAALTHLEDNRLPMRVRGSVADMFDTLAPEMRGFNRVVLSNLWLF
KPLLLREFAKSGTTEAMVRTTTALTVFNAGDKDNVLPGIAEASVNFRLLPGDTQAGITDHVRKTVANDRIAIAGSEGNFD
PPPVTGTASASYEALNRTIREIFPDVVVAPGLMIAATDSRHYAGVADNIFRFSPVRATSEDLKRFHGTNERISIANYADM
IRFYVRLIQNTAG

Sequences:

>Translated_493_residues
MRRLLRWIRNIVVVAVIAIAGLAVALTYNTFRQPSRQIDVAAVAPVAVDEDGAARRLAEAIRFPTISNFLNPEQDAEALR
GLQAHIVASFPAFHAAATREVVNGKSLLYTWQGTDPQARPIALLAHQDVVPIAPKTEQDWQHKPFDGVIADGFVWGRGSW
DDKGNLYAMLEAVEAMAKQGFRPKRTIYFAFGHDEEVSGLRGARQIADLLAARKVRLDFVLDEGLLITDGIMKGLDRPAA
LIGVSEKGYATLVLTARGTPGHSSMPPRDTAIGMLAAALTHLEDNRLPMRVRGSVADMFDTLAPEMRGFNRVVLSNLWLF
KPLLLREFAKSGTTEAMVRTTTALTVFNAGDKDNVLPGIAEASVNFRLLPGDTQAGITDHVRKTVANDRIAIAGSEGNFD
PPPVTGTASASYEALNRTIREIFPDVVVAPGLMIAATDSRHYAGVADNIFRFSPVRATSEDLKRFHGTNERISIANYADM
IRFYVRLIQNTAG
>Mature_493_residues
MRRLLRWIRNIVVVAVIAIAGLAVALTYNTFRQPSRQIDVAAVAPVAVDEDGAARRLAEAIRFPTISNFLNPEQDAEALR
GLQAHIVASFPAFHAAATREVVNGKSLLYTWQGTDPQARPIALLAHQDVVPIAPKTEQDWQHKPFDGVIADGFVWGRGSW
DDKGNLYAMLEAVEAMAKQGFRPKRTIYFAFGHDEEVSGLRGARQIADLLAARKVRLDFVLDEGLLITDGIMKGLDRPAA
LIGVSEKGYATLVLTARGTPGHSSMPPRDTAIGMLAAALTHLEDNRLPMRVRGSVADMFDTLAPEMRGFNRVVLSNLWLF
KPLLLREFAKSGTTEAMVRTTTALTVFNAGDKDNVLPGIAEASVNFRLLPGDTQAGITDHVRKTVANDRIAIAGSEGNFD
PPPVTGTASASYEALNRTIREIFPDVVVAPGLMIAATDSRHYAGVADNIFRFSPVRATSEDLKRFHGTNERISIANYADM
IRFYVRLIQNTAG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI269846968, Length=440, Percent_Identity=40.6818181818182, Blast_Score=315, Evalue=6e-86,
Organism=Homo sapiens, GI4501901, Length=384, Percent_Identity=27.34375, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI1788816, Length=413, Percent_Identity=23.728813559322, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982710, Length=393, Percent_Identity=25.9541984732824, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538640, Length=175, Percent_Identity=31.4285714285714, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71982082, Length=467, Percent_Identity=23.1263383297645, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71982085, Length=335, Percent_Identity=24.4776119402985, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538642, Length=391, Percent_Identity=23.0179028132992, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322289, Length=488, Percent_Identity=29.0983606557377, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24649202, Length=386, Percent_Identity=27.4611398963731, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24649204, Length=386, Percent_Identity=27.4611398963731, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24649206, Length=392, Percent_Identity=24.234693877551, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24649210, Length=447, Percent_Identity=24.6085011185682, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356353, Length=455, Percent_Identity=23.0769230769231, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24649212, Length=455, Percent_Identity=22.6373626373626, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24649208, Length=395, Percent_Identity=24.5569620253165, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24585880, Length=437, Percent_Identity=23.5697940503432, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 53834; Mature: 53834

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLLRWIRNIVVVAVIAIAGLAVALTYNTFRQPSRQIDVAAVAPVAVDEDGAARRLAEA
CHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCCEEEEEECCEEECCCCHHHHHHHH
IRFPTISNFLNPEQDAEALRGLQAHIVASFPAFHAAATREVVNGKSLLYTWQGTDPQARP
HCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
IALLAHQDVVPIAPKTEQDWQHKPFDGVIADGFVWGRGSWDDKGNLYAMLEAVEAMAKQG
EEEEECCCCCCCCCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCHHHHHHHHHHHHHCC
FRPKRTIYFAFGHDEEVSGLRGARQIADLLAARKVRLDFVLDEGLLITDGIMKGLDRPAA
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEHHHHHCCCCCEE
LIGVSEKGYATLVLTARGTPGHSSMPPRDTAIGMLAAALTHLEDNRLPMRVRGSVADMFD
EEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHH
TLAPEMRGFNRVVLSNLWLFKPLLLREFAKSGTTEAMVRTTTALTVFNAGDKDNVLPGIA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEEEEECCCCCCCCCCCH
EASVNFRLLPGDTQAGITDHVRKTVANDRIAIAGSEGNFDPPPVTGTASASYEALNRTIR
HCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
EIFPDVVVAPGLMIAATDSRHYAGVADNIFRFSPVRATSEDLKRFHGTNERISIANYADM
HHCCHHHHCCCEEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHCCCCCEEEHHHHHHH
IRFYVRLIQNTAG
HHHHHHHHHHCCC
>Mature Secondary Structure
MRRLLRWIRNIVVVAVIAIAGLAVALTYNTFRQPSRQIDVAAVAPVAVDEDGAARRLAEA
CHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCCEEEEEECCEEECCCCHHHHHHHH
IRFPTISNFLNPEQDAEALRGLQAHIVASFPAFHAAATREVVNGKSLLYTWQGTDPQARP
HCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
IALLAHQDVVPIAPKTEQDWQHKPFDGVIADGFVWGRGSWDDKGNLYAMLEAVEAMAKQG
EEEEECCCCCCCCCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCHHHHHHHHHHHHHCC
FRPKRTIYFAFGHDEEVSGLRGARQIADLLAARKVRLDFVLDEGLLITDGIMKGLDRPAA
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEHHHHHCCCCCEE
LIGVSEKGYATLVLTARGTPGHSSMPPRDTAIGMLAAALTHLEDNRLPMRVRGSVADMFD
EEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHH
TLAPEMRGFNRVVLSNLWLFKPLLLREFAKSGTTEAMVRTTTALTVFNAGDKDNVLPGIA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEEEEECCCCCCCCCCCH
EASVNFRLLPGDTQAGITDHVRKTVANDRIAIAGSEGNFDPPPVTGTASASYEALNRTIR
HCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
EIFPDVVVAPGLMIAATDSRHYAGVADNIFRFSPVRATSEDLKRFHGTNERISIANYADM
HHCCHHHHCCCEEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHCCCCCEEEHHHHHHH
IRFYVRLIQNTAG
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]