Click here to switch to the map view.

The map label for this gene is 86747190

Identifier: 86747190

GI number: 86747190

Start: 66038

End: 67363

Strand: Direct

Name: 86747190

Synonym: RPB_0063

Alternate gene names: NA

Gene position: 66038-67363 (Clockwise)

Preceding gene: 86747189

Following gene: 86747191

Centisome position: 1.24

GC content: 63.42

Gene sequence:

>1326_bases
ATGCCCGTGATTGGTGAATTTGGCCGTCCGCCGGAGCTGCCGGCCGAAGTCAGTCCGGCGATGACCACGCCGATGTACTG
GTTCTACGAACTCGCTCACGCCTCGCTCAATCCGGCGCGCGCGATGTCGGACGCAGCAAAGCTGATGTTCAAGAATCCGC
TGAACCCGTGGAGTCACACCGATCTCGGCAAGTCGATCGCGGCGGCCTGCGAAGTGTTCGAGCGTACCACGCGCCGTTAC
GGCAAGCCGGAATGGGGCCTCGACACCACCGAGGTCAACGGCATGCGGGTGCCGATCGAAATTCACAATGTGTGGGAAAA
GCCGTTCTGCCGGCTGTTGTATTTCGAACGCAAGCTGGAACACCCGCTGCGCCATCCGCAGCCGCGGGTGCTGATCGTGG
CGCCGATGTCGGGTCACTACGCGACGCTGCTGCGCGGCACCGTCGAGGCCTTCCTGCCGACCCACGAAGTGTACATCACC
GACTGGACCGATGCCCGGATGGTGCCGGTCGCCGCCGGCCGCTTCGACCTCGACGACTATGTCGACTATGTCATCGAGAT
GCTGCAGACGCTGGGCGGCAACGTCCACGTCGTCGCGGTGTGCCAGCCCTCGGTGCCGGTGCTGGCCGCGGTCTCGGTGA
TGGAAGCCAACGACGATCCGTTCGTGCCGCTGTCGATGACGCTGATGGGCGGCCCGATCGACACCCGCCGCAATCCGACC
GCGGTCAACAATCTCGCCGCCGAGAAGGGCATCGACTGGTTCCGCAGCCACGTCATCACCAAGGTGCCGTTCCCGCATCC
GGGCGTGATGCGCGACGTCTATCCGGGATTCCTGCAGCTCAATGGCTTCATCAGCATGAATCTCGACCGCCACATGGACG
CGCACAAGAACCTGTTCAACCATCTGGTTCAGGGCGACGGCGACCTCGCCGACAAGCATCGCGAGTTCTACGACGAATAT
CTGGCGGTGATGGACCTGACCGCGGAGTTCTATCTGCAGACCGTCGATGTGGTGTTCGTCAAGCACGCGCTGCCGAAGGG
CGAGATGATGCATCGGGGCAAGCTGGTCGAGCCGTGGAAGATCAAGCGCGTGGCGCTGATGACGGTCGAAGGCGAGAAGG
ACGACATCTCCGGCCTCGGACAGACCGAAGCGACGCACACGCTGTGCAGCTCGATTCCGGACGAGCGCCGCGTGCACTAC
GTGCAGAAGGGCGTCGGCCATTACGGCGTGTTCAACGGTTCGCGGTTCCGATCCGAAATCGTGCCGCGCATTTCCGATTT
CATGCTGTCGGCCGCGAACGGCGCACGCGCTAACGCGGCGGAGTAA

Upstream 100 bases:

>100_bases
TGGCCGCTGTCTGTGCTCATAATTCGATCTACCGATTGACGCACCCAGACCCAGCGGCCACTATGCTGCGGTGCGAGAAG
CGACCAGGGAAACAAATACG

Downstream 100 bases:

>100_bases
CAGGCGGCGCGGCGATCGCTGTGAAGATCGGGGATCGGCCTCGTCGCGCGCTCGGTTTACATCGAGGAATCAAGTGGAAG
CGCCGAGCCGAGGCAGTTCG

Product: polyhydroxyalkanoate depolymerase, intracellular

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Poly(3-Hydroxyalkanoate)Synthetase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MPVIGEFGRPPELPAEVSPAMTTPMYWFYELAHASLNPARAMSDAAKLMFKNPLNPWSHTDLGKSIAAACEVFERTTRRY
GKPEWGLDTTEVNGMRVPIEIHNVWEKPFCRLLYFERKLEHPLRHPQPRVLIVAPMSGHYATLLRGTVEAFLPTHEVYIT
DWTDARMVPVAAGRFDLDDYVDYVIEMLQTLGGNVHVVAVCQPSVPVLAAVSVMEANDDPFVPLSMTLMGGPIDTRRNPT
AVNNLAAEKGIDWFRSHVITKVPFPHPGVMRDVYPGFLQLNGFISMNLDRHMDAHKNLFNHLVQGDGDLADKHREFYDEY
LAVMDLTAEFYLQTVDVVFVKHALPKGEMMHRGKLVEPWKIKRVALMTVEGEKDDISGLGQTEATHTLCSSIPDERRVHY
VQKGVGHYGVFNGSRFRSEIVPRISDFMLSAANGARANAAE

Sequences:

>Translated_441_residues
MPVIGEFGRPPELPAEVSPAMTTPMYWFYELAHASLNPARAMSDAAKLMFKNPLNPWSHTDLGKSIAAACEVFERTTRRY
GKPEWGLDTTEVNGMRVPIEIHNVWEKPFCRLLYFERKLEHPLRHPQPRVLIVAPMSGHYATLLRGTVEAFLPTHEVYIT
DWTDARMVPVAAGRFDLDDYVDYVIEMLQTLGGNVHVVAVCQPSVPVLAAVSVMEANDDPFVPLSMTLMGGPIDTRRNPT
AVNNLAAEKGIDWFRSHVITKVPFPHPGVMRDVYPGFLQLNGFISMNLDRHMDAHKNLFNHLVQGDGDLADKHREFYDEY
LAVMDLTAEFYLQTVDVVFVKHALPKGEMMHRGKLVEPWKIKRVALMTVEGEKDDISGLGQTEATHTLCSSIPDERRVHY
VQKGVGHYGVFNGSRFRSEIVPRISDFMLSAANGARANAAE
>Mature_440_residues
PVIGEFGRPPELPAEVSPAMTTPMYWFYELAHASLNPARAMSDAAKLMFKNPLNPWSHTDLGKSIAAACEVFERTTRRYG
KPEWGLDTTEVNGMRVPIEIHNVWEKPFCRLLYFERKLEHPLRHPQPRVLIVAPMSGHYATLLRGTVEAFLPTHEVYITD
WTDARMVPVAAGRFDLDDYVDYVIEMLQTLGGNVHVVAVCQPSVPVLAAVSVMEANDDPFVPLSMTLMGGPIDTRRNPTA
VNNLAAEKGIDWFRSHVITKVPFPHPGVMRDVYPGFLQLNGFISMNLDRHMDAHKNLFNHLVQGDGDLADKHREFYDEYL
AVMDLTAEFYLQTVDVVFVKHALPKGEMMHRGKLVEPWKIKRVALMTVEGEKDDISGLGQTEATHTLCSSIPDERRVHYV
QKGVGHYGVFNGSRFRSEIVPRISDFMLSAANGARANAAE

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 49607; Mature: 49475

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVIGEFGRPPELPAEVSPAMTTPMYWFYELAHASLNPARAMSDAAKLMFKNPLNPWSHT
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
DLGKSIAAACEVFERTTRRYGKPEWGLDTTEVNGMRVPIEIHNVWEKPFCRLLYFERKLE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHCHHHHHHHHHHHHHHHHH
HPLRHPQPRVLIVAPMSGHYATLLRGTVEAFLPTHEVYITDWTDARMVPVAAGRFDLDDY
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCHHHH
VDYVIEMLQTLGGNVHVVAVCQPSVPVLAAVSVMEANDDPFVPLSMTLMGGPIDTRRNPT
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCC
AVNNLAAEKGIDWFRSHVITKVPFPHPGVMRDVYPGFLQLNGFISMNLDRHMDAHKNLFN
HHHHHHHHHCHHHHHHCCEEECCCCCCCCHHHHCCCCEEECCEEEECHHHHHHHHHHHHH
HLVQGDGDLADKHREFYDEYLAVMDLTAEFYLQTVDVVFVKHALPKGEMMHRGKLVEPWK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCE
IKRVALMTVEGEKDDISGLGQTEATHTLCSSIPDERRVHYVQKGVGHYGVFNGSRFRSEI
EEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHH
VPRISDFMLSAANGARANAAE
HHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PVIGEFGRPPELPAEVSPAMTTPMYWFYELAHASLNPARAMSDAAKLMFKNPLNPWSHT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
DLGKSIAAACEVFERTTRRYGKPEWGLDTTEVNGMRVPIEIHNVWEKPFCRLLYFERKLE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHCHHHHHHHHHHHHHHHHH
HPLRHPQPRVLIVAPMSGHYATLLRGTVEAFLPTHEVYITDWTDARMVPVAAGRFDLDDY
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCHHHH
VDYVIEMLQTLGGNVHVVAVCQPSVPVLAAVSVMEANDDPFVPLSMTLMGGPIDTRRNPT
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCC
AVNNLAAEKGIDWFRSHVITKVPFPHPGVMRDVYPGFLQLNGFISMNLDRHMDAHKNLFN
HHHHHHHHHCHHHHHHCCEEECCCCCCCCHHHHCCCCEEECCEEEECHHHHHHHHHHHHH
HLVQGDGDLADKHREFYDEYLAVMDLTAEFYLQTVDVVFVKHALPKGEMMHRGKLVEPWK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCE
IKRVALMTVEGEKDDISGLGQTEATHTLCSSIPDERRVHYVQKGVGHYGVFNGSRFRSEI
EEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHH
VPRISDFMLSAANGARANAAE
HHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA