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The map label for this gene is map [H]

Identifier: 86747185

GI number: 86747185

Start: 61107

End: 61931

Strand: Direct

Name: map [H]

Synonym: RPB_0058

Alternate gene names: 86747185

Gene position: 61107-61931 (Clockwise)

Preceding gene: 86747184

Following gene: 86747188

Centisome position: 1.15

GC content: 64.48

Gene sequence:

>825_bases
ATGAGCTACGTGGACGCCTCCGACACCGCCCTGCGCAAGACCGGGCAGATCAAATTGCACGGCGCCGCCGGTTTCGCCGG
CATGCGCAAGGCCGGTCAGGTCGTGGCGCAATGCCTCGACGAACTGGTCGACATCGTCAAACCCGGGGTGCCGACCTCGG
CGATCGACGATTTCGTCCGCGATTTCGCCTTCAGCCACGGTGCCTTTCCGGCGACGCTGATGTATCGCGGCTATCGCTAT
TCGACCTGCACCTCGATCAATCACGTGGTCTGCCACGGCATGCCGGGCGACCGGCCGCTGAAGGAAGGCGACATCGTCAA
TGTCGACGTGACGATGATCGTCGACGGCTGGTACGGCGATTCCAGCCGGATGTATCCGGTCGGTCCGATTGCGCGCAAAG
CCGAGCGGCTGATCGACGTCACCTACGAGTCGCTGCTGCGCGGCATCGCGGCGGTCAAGCCGGGCGCCACCACCGGCGAC
ATCGGGCACGCGATCCAGAGTTTCGTCGAGCCGCAGCAGATGAGCGTGGTGCGGGATTTCTGCGGCCACGGCCTCGGCCG
GCTGTTCCACGATGAGCCGAACATCATCCATGTCGGCTCGCCCGGCCAGGGCGTTGTGCTGAAGCCCGGCATGTTCTTCA
CCATCGAGCCGATGATCAATCTCGGCAAACCCTATGTGAAGATCCTGTCCGACGGCTGGACCGCGGTGACCCGCGACCGC
TCGCTGTCGGCGCAGTTCGAGCACTCGATCGGCGTCACCAAGGACGGCTGCGAGATCTTCACGCTGTCGCCCAAGCAACT
CGACAAGCCGCCGTTCCAGAGCTGA

Upstream 100 bases:

>100_bases
GAGCCGTTCCGGCGCGCCCGCTGGGGCTTTTGCTTGCGCCCACCGCTGCGATGACTAAATTGGCCTCACATCCTCCGTTT
TCCTCAACCAGACCGGCCCG

Downstream 100 bases:

>100_bases
GATCCTTCCGATCGTCGCAAAGGCCAACGTCCCCGCGCGCCGCAGCGCGCCCCCTCGCCCGCTTGCGGGAGAGGGTTGGG
GTGAGGGCGAGCCGAGCACG

Product: methionine aminopeptidase, type I

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSYVDASDTALRKTGQIKLHGAAGFAGMRKAGQVVAQCLDELVDIVKPGVPTSAIDDFVRDFAFSHGAFPATLMYRGYRY
STCTSINHVVCHGMPGDRPLKEGDIVNVDVTMIVDGWYGDSSRMYPVGPIARKAERLIDVTYESLLRGIAAVKPGATTGD
IGHAIQSFVEPQQMSVVRDFCGHGLGRLFHDEPNIIHVGSPGQGVVLKPGMFFTIEPMINLGKPYVKILSDGWTAVTRDR
SLSAQFEHSIGVTKDGCEIFTLSPKQLDKPPFQS

Sequences:

>Translated_274_residues
MSYVDASDTALRKTGQIKLHGAAGFAGMRKAGQVVAQCLDELVDIVKPGVPTSAIDDFVRDFAFSHGAFPATLMYRGYRY
STCTSINHVVCHGMPGDRPLKEGDIVNVDVTMIVDGWYGDSSRMYPVGPIARKAERLIDVTYESLLRGIAAVKPGATTGD
IGHAIQSFVEPQQMSVVRDFCGHGLGRLFHDEPNIIHVGSPGQGVVLKPGMFFTIEPMINLGKPYVKILSDGWTAVTRDR
SLSAQFEHSIGVTKDGCEIFTLSPKQLDKPPFQS
>Mature_273_residues
SYVDASDTALRKTGQIKLHGAAGFAGMRKAGQVVAQCLDELVDIVKPGVPTSAIDDFVRDFAFSHGAFPATLMYRGYRYS
TCTSINHVVCHGMPGDRPLKEGDIVNVDVTMIVDGWYGDSSRMYPVGPIARKAERLIDVTYESLLRGIAAVKPGATTGDI
GHAIQSFVEPQQMSVVRDFCGHGLGRLFHDEPNIIHVGSPGQGVVLKPGMFFTIEPMINLGKPYVKILSDGWTAVTRDRS
LSAQFEHSIGVTKDGCEIFTLSPKQLDKPPFQS

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=273, Percent_Identity=43.2234432234432, Blast_Score=226, Evalue=1e-59,
Organism=Homo sapiens, GI40385867, Length=250, Percent_Identity=36.8, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1786364, Length=237, Percent_Identity=52.7426160337553, Blast_Score=249, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI71996291, Length=236, Percent_Identity=42.7966101694915, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323273, Length=262, Percent_Identity=39.3129770992366, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI21355531, Length=266, Percent_Identity=41.7293233082707, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24583427, Length=236, Percent_Identity=38.9830508474576, Blast_Score=187, Evalue=6e-48,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 29773; Mature: 29642

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYVDASDTALRKTGQIKLHGAAGFAGMRKAGQVVAQCLDELVDIVKPGVPTSAIDDFVR
CCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DFAFSHGAFPATLMYRGYRYSTCTSINHVVCHGMPGDRPLKEGDIVNVDVTMIVDGWYGD
HHHHCCCCCCHHHHCCCCCCHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEEEEEEEECC
SSRMYPVGPIARKAERLIDVTYESLLRGIAAVKPGATTGDIGHAIQSFVEPQQMSVVRDF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
CGHGLGRLFHDEPNIIHVGSPGQGVVLKPGMFFTIEPMINLGKPYVKILSDGWTAVTRDR
HCCCHHHHCCCCCCEEEECCCCCCEEECCCCEEEECCHHHCCCHHHHHHHCCCEEEECCC
SLSAQFEHSIGVTKDGCEIFTLSPKQLDKPPFQS
CCCHHHHHCCCCCCCCCEEEEECHHHCCCCCCCC
>Mature Secondary Structure 
SYVDASDTALRKTGQIKLHGAAGFAGMRKAGQVVAQCLDELVDIVKPGVPTSAIDDFVR
CCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DFAFSHGAFPATLMYRGYRYSTCTSINHVVCHGMPGDRPLKEGDIVNVDVTMIVDGWYGD
HHHHCCCCCCHHHHCCCCCCHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEEEEEEEECC
SSRMYPVGPIARKAERLIDVTYESLLRGIAAVKPGATTGDIGHAIQSFVEPQQMSVVRDF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
CGHGLGRLFHDEPNIIHVGSPGQGVVLKPGMFFTIEPMINLGKPYVKILSDGWTAVTRDR
HCCCHHHHCCCCCCEEEECCCCCCEEECCCCEEEECCHHHCCCHHHHHHHCCCEEEECCC
SLSAQFEHSIGVTKDGCEIFTLSPKQLDKPPFQS
CCCHHHHHCCCCCCCCCEEEEECHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]