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The map label for this gene is rhdA [H]

Identifier: 86610136

GI number: 86610136

Start: 2801571

End: 2802398

Strand: Direct

Name: rhdA [H]

Synonym: CYB_2710

Alternate gene names: 86610136

Gene position: 2801571-2802398 (Clockwise)

Preceding gene: 86610135

Following gene: 86610137

Centisome position: 91.95

GC content: 61.47

Gene sequence:

>828_bases
ATGACACCAACGCCAGGTTGGGTGGTCAGCCCTGCAACCGCCATCCAGCTCATCCAACAAGGGGCAACGCTTCTGGATGC
ACGCAGTGCGGAATTGCAAGCCGACGGGATCCTGAAGGGTTCAATTCCGGTGAACTGGACGGAGTTTACTCAACCAGAGG
CTGCAAACAGCGGCAAGCTGTTGGCAGATGATGCCCTTCTCACCGAAAGGCTGCAGGGCCTTGGCGTGTTTCAGGGGCGT
CCGGTGGTGGTGGTGGCCGATCCGCTGCGGGGCTGGGGAGAAGATGGCTGGATCGTTTGGATGTTGCGCAGCTTGGGTCA
TCCCCAGGCAGTCATGGTGGACGGGGGCTATCCGGCCCTGGTGAAGGAGGGAGTGCTGACCACAACCCGACAAAGCGCCG
ACGAACCGCAACGGGGCGACTTTGTAGTGCAGCGAACCGACCGGTGGGAGATCGACCGGGACACTCTGCGGGCCAGCCTT
GGCTCCGACCGTGTGGCCATCCTCGACAGTCGCGAAGCCCAAGAATACAAGGGAGCCACCCCCTATGGTGAGGCGCGCGG
TGGACACATCCCCGGCGCTGTGCATCTCTACTACAAGGAGCTGATGGATGCGGCGGGATCCCTATTGCCGCCCGATGAAA
TTCACGCCCGCCTGCAAAAAGCCGGGATCCCTCCAGAAGCCGAGATCATTGCCTACTGCACAGCTGGGGTTCGCTCCGCT
TGGGTCACGGTGGTTTTAACCCATTTGGGCCGGCAGGCCAAAAGCTATTCCGGTTCCATCCTGGAATGGGCAGCCGGGGA
TCTCCAGAAGTTCCCGCTCCAGCCATGA

Upstream 100 bases:

>100_bases
GCCTGCAACGGCTTCTCTCCAAACAGATGGAAGAGGCCCATACCGGCCTACTCGTCCCGTAAGGTGGGATAGGATCTTCC
AGTTAAGGTTCTCTCAACCC

Downstream 100 bases:

>100_bases
GGGGATCCCTGCCCACTGTGATCTTGGCCGGATGTGGCTATGGAGCCATCGACGCCTTGCGGGCGCTCAAAGGGCAGGCA
CGGGTGATCGCCATCAACCC

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTPTPGWVVSPATAIQLIQQGATLLDARSAELQADGILKGSIPVNWTEFTQPEAANSGKLLADDALLTERLQGLGVFQGR
PVVVVADPLRGWGEDGWIVWMLRSLGHPQAVMVDGGYPALVKEGVLTTTRQSADEPQRGDFVVQRTDRWEIDRDTLRASL
GSDRVAILDSREAQEYKGATPYGEARGGHIPGAVHLYYKELMDAAGSLLPPDEIHARLQKAGIPPEAEIIAYCTAGVRSA
WVTVVLTHLGRQAKSYSGSILEWAAGDLQKFPLQP

Sequences:

>Translated_275_residues
MTPTPGWVVSPATAIQLIQQGATLLDARSAELQADGILKGSIPVNWTEFTQPEAANSGKLLADDALLTERLQGLGVFQGR
PVVVVADPLRGWGEDGWIVWMLRSLGHPQAVMVDGGYPALVKEGVLTTTRQSADEPQRGDFVVQRTDRWEIDRDTLRASL
GSDRVAILDSREAQEYKGATPYGEARGGHIPGAVHLYYKELMDAAGSLLPPDEIHARLQKAGIPPEAEIIAYCTAGVRSA
WVTVVLTHLGRQAKSYSGSILEWAAGDLQKFPLQP
>Mature_274_residues
TPTPGWVVSPATAIQLIQQGATLLDARSAELQADGILKGSIPVNWTEFTQPEAANSGKLLADDALLTERLQGLGVFQGRP
VVVVADPLRGWGEDGWIVWMLRSLGHPQAVMVDGGYPALVKEGVLTTTRQSADEPQRGDFVVQRTDRWEIDRDTLRASLG
SDRVAILDSREAQEYKGATPYGEARGGHIPGAVHLYYKELMDAAGSLLPPDEIHARLQKAGIPPEAEIIAYCTAGVRSAW
VTVVLTHLGRQAKSYSGSILEWAAGDLQKFPLQP

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=214, Percent_Identity=28.5046728971963, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI61835204, Length=195, Percent_Identity=25.6410256410256, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI194473681, Length=195, Percent_Identity=25.6410256410256, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI194473668, Length=195, Percent_Identity=25.6410256410256, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 29754; Mature: 29623

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTPGWVVSPATAIQLIQQGATLLDARSAELQADGILKGSIPVNWTEFTQPEAANSGKL
CCCCCCEEECHHHHHHHHHHCCHHHHCCCCCCCCCCEEECCCCCCHHHCCCCCCCCCCCE
LADDALLTERLQGLGVFQGRPVVVVADPLRGWGEDGWIVWMLRSLGHPQAVMVDGGYPAL
EEHHHHHHHHHHHCCEECCCCEEEEECCCCCCCCCCEEEEEEHHCCCCCEEEEECCCHHH
VKEGVLTTTRQSADEPQRGDFVVQRTDRWEIDRDTLRASLGSDRVAILDSREAQEYKGAT
HHCCCHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEECCHHHHHCCCC
PYGEARGGHIPGAVHLYYKELMDAAGSLLPPDEIHARLQKAGIPPEAEIIAYCTAGVRSA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHEEEEEHHHHHHH
WVTVVLTHLGRQAKSYSGSILEWAAGDLQKFPLQP
HHHHHHHHHHHHHHHCCCCCHHHHCCCHHHCCCCC
>Mature Secondary Structure 
TPTPGWVVSPATAIQLIQQGATLLDARSAELQADGILKGSIPVNWTEFTQPEAANSGKL
CCCCCEEECHHHHHHHHHHCCHHHHCCCCCCCCCCEEECCCCCCHHHCCCCCCCCCCCE
LADDALLTERLQGLGVFQGRPVVVVADPLRGWGEDGWIVWMLRSLGHPQAVMVDGGYPAL
EEHHHHHHHHHHHCCEECCCCEEEEECCCCCCCCCCEEEEEEHHCCCCCEEEEECCCHHH
VKEGVLTTTRQSADEPQRGDFVVQRTDRWEIDRDTLRASLGSDRVAILDSREAQEYKGAT
HHCCCHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEECCHHHHHCCCC
PYGEARGGHIPGAVHLYYKELMDAAGSLLPPDEIHARLQKAGIPPEAEIIAYCTAGVRSA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHEEEEEHHHHHHH
WVTVVLTHLGRQAKSYSGSILEWAAGDLQKFPLQP
HHHHHHHHHHHHHHHCCCCCHHHHCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]