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The map label for this gene is ispH [H]

Identifier: 86610071

GI number: 86610071

Start: 2730695

End: 2731954

Strand: Direct

Name: ispH [H]

Synonym: CYB_2643

Alternate gene names: 86610071

Gene position: 2730695-2731954 (Clockwise)

Preceding gene: 86610070

Following gene: 86610072

Centisome position: 89.63

GC content: 57.7

Gene sequence:

>1260_bases
ATGCCTCATTCCCAACCCACTTCCCCGGATCCCTCTCCCCAAGACCCACGGGCCCTGCGCAAAGCCCTGCGCGGATCCAG
CACCTACTTTGCCAAGGGATTTGAAGAACACAAGGATCAGGCTCAACTGGAGCTGCAAACCACCTACAAAAGCCCCCTTG
TGGAGCAAATCCGTGCCCAGTCCTACCGCTATCAGCGGGGCGACATTACCATTCACCTGGCCAGGGCCTTTGGCTTTTGC
TGGGGCGTGGAACGGGCGGTGGAATATGCCTATGAAGCGCGGCGACGCTTTCCGGATCGGCAACTGTGGATCACCAACGA
GATCATCCACAACCCTTTGGTAAACCAGCACCTGAAGGAGATGGGGATCCGCTTTGTGGAGAAAGGCCCGGACGGCAAGA
AGGACTTTTCTCAGATCCGGCCTCAAGATGTGGTGATCTGGCCTGCTTTTGGGGCGAGTGCCTCGGAAATGGCGTTTTTC
CGGTCGCAGGGCAATGAGATCGTCGATACCACCTGCCCCTGGGTTTCCCGCGTGTGGAACCAAGTGGACAAGCACCGCGC
GGCTCAATTCACCTCTGTGATCCACGGCAAGTACAACCACGAGGAAACCGTAGCCACCAGCTCCTTTGCCGAAAAATATC
TGGTGGTGCTGAACCTGGAAGAAGCAGAATGGGTCGCTCGCTACATTTTGCAAGGCGGAGATGCGGCAGAATTTCGGCGG
CGCTTTGGCAAGGCCACTTCCCCCGGTTTTGATCCGGATCGCGATCTAGAGCGGATCGGCATCGCCAACCAAACCACGAT
GCTGGAAGGGGAGACCCGCGCCATTGCCAAGCTGTTTGAGACCACCATGCTGCGCAAATATGGCCCCCAGGAGTTAGACA
ACCACTTCATGAGCTTCAACACCATCTGCAATGCCACCCAAGAGCGGCAGGACGCCATGTTCGAGCTGCTCGAGCAGCCT
TTGGATGTCGTGGTGGTGATCGGGGGGTTTAATTCCTCGAATACCACCCACTTGCAGGAAATTGCCCTGGCCAAAGGCCT
GCCCTCCTTCCATATCGATGGTCCTGACTGCATCCAACCCAACAACCGCATCCGCCACAAGCCCCTTCACCAAGACCCGA
CAGTTACCGAAGGGTGGCTACCCGATGGGCCGGTGCAAATCGGCATTACCTCCGGTGCCTCTACCCCAGACCGCGTTGTT
GAAGAGGTGATTCAACGCATCTTCGCCCTGCGTGAAGAACCTCGGCGGATCCCTGCCTAG

Upstream 100 bases:

>100_bases
GGAAAGCGTGCCGAAATAGCTCGCCGACTGAGACTGGGCGTAAGCTGAGAAAGGATCCCTTCGCCGTCTGCTTGATCAGT
GCGTATCTCCAACCGTAACC

Downstream 100 bases:

>100_bases
GGATCCCTTCCCTCTTCCCTTTGAGCGTGCTCAACACCCTTTTTCAGGTTTATCCTCCCTTGCTGGGCTGGACGCTGCTG
GGGTTTGCTCTCCAGAAGGG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPHSQPTSPDPSPQDPRALRKALRGSSTYFAKGFEEHKDQAQLELQTTYKSPLVEQIRAQSYRYQRGDITIHLARAFGFC
WGVERAVEYAYEARRRFPDRQLWITNEIIHNPLVNQHLKEMGIRFVEKGPDGKKDFSQIRPQDVVIWPAFGASASEMAFF
RSQGNEIVDTTCPWVSRVWNQVDKHRAAQFTSVIHGKYNHEETVATSSFAEKYLVVLNLEEAEWVARYILQGGDAAEFRR
RFGKATSPGFDPDRDLERIGIANQTTMLEGETRAIAKLFETTMLRKYGPQELDNHFMSFNTICNATQERQDAMFELLEQP
LDVVVVIGGFNSSNTTHLQEIALAKGLPSFHIDGPDCIQPNNRIRHKPLHQDPTVTEGWLPDGPVQIGITSGASTPDRVV
EEVIQRIFALREEPRRIPA

Sequences:

>Translated_419_residues
MPHSQPTSPDPSPQDPRALRKALRGSSTYFAKGFEEHKDQAQLELQTTYKSPLVEQIRAQSYRYQRGDITIHLARAFGFC
WGVERAVEYAYEARRRFPDRQLWITNEIIHNPLVNQHLKEMGIRFVEKGPDGKKDFSQIRPQDVVIWPAFGASASEMAFF
RSQGNEIVDTTCPWVSRVWNQVDKHRAAQFTSVIHGKYNHEETVATSSFAEKYLVVLNLEEAEWVARYILQGGDAAEFRR
RFGKATSPGFDPDRDLERIGIANQTTMLEGETRAIAKLFETTMLRKYGPQELDNHFMSFNTICNATQERQDAMFELLEQP
LDVVVVIGGFNSSNTTHLQEIALAKGLPSFHIDGPDCIQPNNRIRHKPLHQDPTVTEGWLPDGPVQIGITSGASTPDRVV
EEVIQRIFALREEPRRIPA
>Mature_418_residues
PHSQPTSPDPSPQDPRALRKALRGSSTYFAKGFEEHKDQAQLELQTTYKSPLVEQIRAQSYRYQRGDITIHLARAFGFCW
GVERAVEYAYEARRRFPDRQLWITNEIIHNPLVNQHLKEMGIRFVEKGPDGKKDFSQIRPQDVVIWPAFGASASEMAFFR
SQGNEIVDTTCPWVSRVWNQVDKHRAAQFTSVIHGKYNHEETVATSSFAEKYLVVLNLEEAEWVARYILQGGDAAEFRRR
FGKATSPGFDPDRDLERIGIANQTTMLEGETRAIAKLFETTMLRKYGPQELDNHFMSFNTICNATQERQDAMFELLEQPL
DVVVVIGGFNSSNTTHLQEIALAKGLPSFHIDGPDCIQPNNRIRHKPLHQDPTVTEGWLPDGPVQIGITSGASTPDRVVE
EVIQRIFALREEPRRIPA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=342, Percent_Identity=26.3157894736842, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 47716; Mature: 47585

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHSQPTSPDPSPQDPRALRKALRGSSTYFAKGFEEHKDQAQLELQTTYKSPLVEQIRAQ
CCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCHHHHHHHHHEEHHHHHHHHHHHHHHHH
SYRYQRGDITIHLARAFGFCWGVERAVEYAYEARRRFPDRQLWITNEIIHNPLVNQHLKE
HHCEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHCHHHHHHHHH
MGIRFVEKGPDGKKDFSQIRPQDVVIWPAFGASASEMAFFRSQGNEIVDTTCPWVSRVWN
HHHHHHHCCCCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEECCCCHHHHHHHH
QVDKHRAAQFTSVIHGKYNHEETVATSSFAEKYLVVLNLEEAEWVARYILQGGDAAEFRR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEEEEEECCHHHHHHHHHHCCCCHHHHHH
RFGKATSPGFDPDRDLERIGIANQTTMLEGETRAIAKLFETTMLRKYGPQELDNHFMSFN
HHCCCCCCCCCCCCHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TICNATQERQDAMFELLEQPLDVVVVIGGFNSSNTTHLQEIALAKGLPSFHIDGPDCIQP
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
NNRIRHKPLHQDPTVTEGWLPDGPVQIGITSGASTPDRVVEEVIQRIFALREEPRRIPA
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PHSQPTSPDPSPQDPRALRKALRGSSTYFAKGFEEHKDQAQLELQTTYKSPLVEQIRAQ
CCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCHHHHHHHHHEEHHHHHHHHHHHHHHHH
SYRYQRGDITIHLARAFGFCWGVERAVEYAYEARRRFPDRQLWITNEIIHNPLVNQHLKE
HHCEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHCHHHHHHHHH
MGIRFVEKGPDGKKDFSQIRPQDVVIWPAFGASASEMAFFRSQGNEIVDTTCPWVSRVWN
HHHHHHHCCCCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEECCCCHHHHHHHH
QVDKHRAAQFTSVIHGKYNHEETVATSSFAEKYLVVLNLEEAEWVARYILQGGDAAEFRR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEEEEEECCHHHHHHHHHHCCCCHHHHHH
RFGKATSPGFDPDRDLERIGIANQTTMLEGETRAIAKLFETTMLRKYGPQELDNHFMSFN
HHCCCCCCCCCCCCHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TICNATQERQDAMFELLEQPLDVVVVIGGFNSSNTTHLQEIALAKGLPSFHIDGPDCIQP
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
NNRIRHKPLHQDPTVTEGWLPDGPVQIGITSGASTPDRVVEEVIQRIFALREEPRRIPA
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA