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The map label for this gene is sigF [H]

Identifier: 86609923

GI number: 86609923

Start: 2585421

End: 2586302

Strand: Direct

Name: sigF [H]

Synonym: CYB_2489

Alternate gene names: 86609923

Gene position: 2585421-2586302 (Clockwise)

Preceding gene: 86609922

Following gene: 86609924

Centisome position: 84.86

GC content: 55.9

Gene sequence:

>882_bases
ATGATAACGGCCCCTCGCCTGACCTCTCCCAAGAACTCCGTCTCCCTGAGGCGACCGGCGGCCCCCCCTCCATTGCCTTT
GGCCAAGACCGGATTCCCAGCCGCATGTGGATCTATGGCATCTAGTGAGGATGTTAAAGCCCGAACTGCCGAACTGTTGC
GGCTGTACCACCAGAATCGCGATCCAGCCCTGCGCAACCGGCTGGTGGAAATGAACTTGGGCTTGGTGCGCAAAGAGGCC
CACCACTGGCGCAACCAATGCAATGAGGGGTACGACGACCTGGTGCAAGTTGGCTGTTTGGGTTTGATTCAAGCTATCGA
ACGCTTTGACATCAGCCGAGGCCTGGCTTTTAGCTCCTTTGCCCTGCCCTACATTCGCGGAGAAATTCAACACTACCTGC
GGGACAAAAGCCCCACCATGCGGATCCCACGCCGCTGGACGACCCTGCAAAGACAAGCCAAGAAGGTGATTGCCCGTCTG
CGGCAAGAGTTGGGCCGTTCTCCCTCTGAGGCCGAGGTGAGGGAAGCCCTGCAACTGACGGAGCAGGAATGGCGAGAGCT
GGAGCTGGCCCAACAAAACAGCCTTTTGCTCAGCCTGGATGCTCCGATTCAAACTGAGGAAGACAGTGTTGCTCTGGGGG
AGGTCGTTCCGGATCAGCGCTACCGCAGTTTTCAACTGGCTGAAGAAGATCGTATTCGCCTACAACATGCCTTGGCTCAG
CTGGAGGAACGTACCCGCAAGATTTTGGAATTTGTCTTCTTCTATGACCTCACGCAAAAAGAAACGGCAGAGCGGCTAGG
GATCAGTGCCGTTACCGTTTCCCGCCAAGTCAAAAAGGGGCTGGAACGCCTGGCTAAGTTGTTGAATCAAGATATTTTTT
GA

Upstream 100 bases:

>100_bases
GTGTTGGTTATGGGTGGGATCCTCAACCTTTGAATGGTTTTCGAGTTCCTTGAGTGCTTGTTCTACAGTTATTTTCGGCA
AATCGGTAAAGTGGTTGTCT

Downstream 100 bases:

>100_bases
GATACAGCCCGTTACAAATTAAGGGATATAAAAGGGTATAAAAATCGTGTTTCCGAGGCTTGACCCCAGTGAGGTTTAGA
ACAAGCAGGTTCATAGTCAT

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MITAPRLTSPKNSVSLRRPAAPPPLPLAKTGFPAACGSMASSEDVKARTAELLRLYHQNRDPALRNRLVEMNLGLVRKEA
HHWRNQCNEGYDDLVQVGCLGLIQAIERFDISRGLAFSSFALPYIRGEIQHYLRDKSPTMRIPRRWTTLQRQAKKVIARL
RQELGRSPSEAEVREALQLTEQEWRELELAQQNSLLLSLDAPIQTEEDSVALGEVVPDQRYRSFQLAEEDRIRLQHALAQ
LEERTRKILEFVFFYDLTQKETAERLGISAVTVSRQVKKGLERLAKLLNQDIF

Sequences:

>Translated_293_residues
MITAPRLTSPKNSVSLRRPAAPPPLPLAKTGFPAACGSMASSEDVKARTAELLRLYHQNRDPALRNRLVEMNLGLVRKEA
HHWRNQCNEGYDDLVQVGCLGLIQAIERFDISRGLAFSSFALPYIRGEIQHYLRDKSPTMRIPRRWTTLQRQAKKVIARL
RQELGRSPSEAEVREALQLTEQEWRELELAQQNSLLLSLDAPIQTEEDSVALGEVVPDQRYRSFQLAEEDRIRLQHALAQ
LEERTRKILEFVFFYDLTQKETAERLGISAVTVSRQVKKGLERLAKLLNQDIF
>Mature_293_residues
MITAPRLTSPKNSVSLRRPAAPPPLPLAKTGFPAACGSMASSEDVKARTAELLRLYHQNRDPALRNRLVEMNLGLVRKEA
HHWRNQCNEGYDDLVQVGCLGLIQAIERFDISRGLAFSSFALPYIRGEIQHYLRDKSPTMRIPRRWTTLQRQAKKVIARL
RQELGRSPSEAEVREALQLTEQEWRELELAQQNSLLLSLDAPIQTEEDSVALGEVVPDQRYRSFQLAEEDRIRLQHALAQ
LEERTRKILEFVFFYDLTQKETAERLGISAVTVSRQVKKGLERLAKLLNQDIF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for normal spore maturation [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=197, Percent_Identity=33.502538071066, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1789448, Length=207, Percent_Identity=29.951690821256, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1789098, Length=239, Percent_Identity=28.0334728033473, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33577; Mature: 33577

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITAPRLTSPKNSVSLRRPAAPPPLPLAKTGFPAACGSMASSEDVKARTAELLRLYHQNR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCC
DPALRNRLVEMNLGLVRKEAHHWRNQCNEGYDDLVQVGCLGLIQAIERFDISRGLAFSSF
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALPYIRGEIQHYLRDKSPTMRIPRRWTTLQRQAKKVIARLRQELGRSPSEAEVREALQLT
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EQEWRELELAQQNSLLLSLDAPIQTEEDSVALGEVVPDQRYRSFQLAEEDRIRLQHALAQ
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHHHHH
LEERTRKILEFVFFYDLTQKETAERLGISAVTVSRQVKKGLERLAKLLNQDIF
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MITAPRLTSPKNSVSLRRPAAPPPLPLAKTGFPAACGSMASSEDVKARTAELLRLYHQNR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCC
DPALRNRLVEMNLGLVRKEAHHWRNQCNEGYDDLVQVGCLGLIQAIERFDISRGLAFSSF
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALPYIRGEIQHYLRDKSPTMRIPRRWTTLQRQAKKVIARLRQELGRSPSEAEVREALQLT
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EQEWRELELAQQNSLLLSLDAPIQTEEDSVALGEVVPDQRYRSFQLAEEDRIRLQHALAQ
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHHHHH
LEERTRKILEFVFFYDLTQKETAERLGISAVTVSRQVKKGLERLAKLLNQDIF
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476207; 12000953 [H]