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The map label for this gene is menE [H]

Identifier: 86609862

GI number: 86609862

Start: 2523864

End: 2525267

Strand: Direct

Name: menE [H]

Synonym: CYB_2428

Alternate gene names: 86609862

Gene position: 2523864-2525267 (Clockwise)

Preceding gene: 86609858

Following gene: 86609864

Centisome position: 82.84

GC content: 61.68

Gene sequence:

>1404_bases
ATGAGGTCAATTGTCTTGTCCGGGGATGGCGGAGACTTGAAGGAGTGCCTGTCCAAACTGGAAGAGCAACTGCGCCCTTT
GGCAGACAAGGCCGTTCCCCTGCTGGTGGCGGACTCGCATCCAGTGCAGGTGTGGGCGGCAGTTTTGACCTGCCAACGCT
TGGGGATCCCGCTGTTCCTGGGTAATCCCCACTGGGGAGAACGGGAATGGGGACAGGCTTTGGCGGATATGGGATCCGGC
TGGTGCTGGCGCCAGGGCCGACTGGAAGGGGATCCCGCCCCCTTGAATCCGCGCCTGCGAGAGTTGCGGGAAGTTTTGCC
CAGCTCCCATTGGCCGTTGATCGGCATCCCCACCGGTGGCTCCGGCGGCCAGATAAAATGGGCCATGCACTCTTGGCTGA
CCCTCAGTGCCTCGGCCAAAGCCCACCAACAGCACTTTGCCGTCGGGCCTGTACACAGCTATTGCCTTCTGCCCTTGTAC
CACGTCAGCGGCTTGATGCAGGCAGTCCGCTCCTGGCTGAGCGGTGGGCAGTGGGTTGTCTGGGATTGGAAACGGCAACA
GCAGGCCAAGGCTTGGGATCCCCCTTTGCCGGGGAGCTTTCTATCGCTGGTGCCCACTCAGTTGCAACGGCTGTTGCTCA
GCGCCCATCCGGGAGTTTTGGAGAGCCTGCGCCAATTTCGGGCCATTTTGGTCGGCGGTGGCCCCACCTGGCCCAGCTTG
CGGCAACAGGCCCGGCAACTTCACCTTCCCCTTGCCCTCACCTACGGTATGACCGAGACCGCCTCCCAGGTGTGTACGTT
GCTGCCAGAAGCCTTTTGGCAAGGGAGCGACAGTCTGGGTCGGCCTCTGCCCCACGCCACGCTCCAGATCCGCAGCGAAA
AAGGGGATCCCCTGCCCAGCCATGCTGTGGGCTACATTCACATTCAGGCAGAGTCGCTGGCACTGGGCTACTACCCGCAC
CTAAGTGCTACGCACCGCTTACGCGAAGGGCAACAGCAAGGCTTTACCCCCGGCGATTTGGGCTATCTAGACCGACAGGG
ATCCCTCTATTGGTTAGGTCGAGCCGATGACCTAATCCTAACCGGCGGGGAAAAGGTGATGGCTGCAGAAGTGGAAGGGG
AGATCCGAGCCAGCGGCTGGGTGGAAGATGTGTGCGTGTTGGGCCTACCGGATGCCGAATGGGGGCAGCGGGTTGTGGCG
GTGGTCGTCCCCAAAACGGGGATCCCCAAAGAGCTGGAAATGCACCTGAAGCAACACCTAAGCCGCCAACTGAGTCCCTT
CAAGCACCCCAAGCACTGGCTGTGGTGTACGGGGATCCCGCGCAACCCTCAGGGCAAAGTCAACCGCCAACAACTCCGGC
AGTGGGCAGCTCAGACGTTGGGGATCCCAACGCAGGCAAGTTAG

Upstream 100 bases:

>100_bases
AAACCTATCCTCACCGTTCCTTGCCAAGGAAATCGAGGGGGGATCCCCTAATAAAATGCAGGGGATCCGCGAAGCCGTTG
AGATGGCATACCCATAGCCC

Downstream 100 bases:

>100_bases
CGGGATCCCTCCCGAATGCCCAGACGGGCTAAATCTCGCTTGATTTCAAAGGCCAATTCTCGGTTGTGCGGCTCCGTGGC
CAAAGCTTTTCTCAAGTAGG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRSIVLSGDGGDLKECLSKLEEQLRPLADKAVPLLVADSHPVQVWAAVLTCQRLGIPLFLGNPHWGEREWGQALADMGSG
WCWRQGRLEGDPAPLNPRLRELREVLPSSHWPLIGIPTGGSGGQIKWAMHSWLTLSASAKAHQQHFAVGPVHSYCLLPLY
HVSGLMQAVRSWLSGGQWVVWDWKRQQQAKAWDPPLPGSFLSLVPTQLQRLLLSAHPGVLESLRQFRAILVGGGPTWPSL
RQQARQLHLPLALTYGMTETASQVCTLLPEAFWQGSDSLGRPLPHATLQIRSEKGDPLPSHAVGYIHIQAESLALGYYPH
LSATHRLREGQQQGFTPGDLGYLDRQGSLYWLGRADDLILTGGEKVMAAEVEGEIRASGWVEDVCVLGLPDAEWGQRVVA
VVVPKTGIPKELEMHLKQHLSRQLSPFKHPKHWLWCTGIPRNPQGKVNRQQLRQWAAQTLGIPTQAS

Sequences:

>Translated_467_residues
MRSIVLSGDGGDLKECLSKLEEQLRPLADKAVPLLVADSHPVQVWAAVLTCQRLGIPLFLGNPHWGEREWGQALADMGSG
WCWRQGRLEGDPAPLNPRLRELREVLPSSHWPLIGIPTGGSGGQIKWAMHSWLTLSASAKAHQQHFAVGPVHSYCLLPLY
HVSGLMQAVRSWLSGGQWVVWDWKRQQQAKAWDPPLPGSFLSLVPTQLQRLLLSAHPGVLESLRQFRAILVGGGPTWPSL
RQQARQLHLPLALTYGMTETASQVCTLLPEAFWQGSDSLGRPLPHATLQIRSEKGDPLPSHAVGYIHIQAESLALGYYPH
LSATHRLREGQQQGFTPGDLGYLDRQGSLYWLGRADDLILTGGEKVMAAEVEGEIRASGWVEDVCVLGLPDAEWGQRVVA
VVVPKTGIPKELEMHLKQHLSRQLSPFKHPKHWLWCTGIPRNPQGKVNRQQLRQWAAQTLGIPTQAS
>Mature_467_residues
MRSIVLSGDGGDLKECLSKLEEQLRPLADKAVPLLVADSHPVQVWAAVLTCQRLGIPLFLGNPHWGEREWGQALADMGSG
WCWRQGRLEGDPAPLNPRLRELREVLPSSHWPLIGIPTGGSGGQIKWAMHSWLTLSASAKAHQQHFAVGPVHSYCLLPLY
HVSGLMQAVRSWLSGGQWVVWDWKRQQQAKAWDPPLPGSFLSLVPTQLQRLLLSAHPGVLESLRQFRAILVGGGPTWPSL
RQQARQLHLPLALTYGMTETASQVCTLLPEAFWQGSDSLGRPLPHATLQIRSEKGDPLPSHAVGYIHIQAESLALGYYPH
LSATHRLREGQQQGFTPGDLGYLDRQGSLYWLGRADDLILTGGEKVMAAEVEGEIRASGWVEDVCVLGLPDAEWGQRVVA
VVVPKTGIPKELEMHLKQHLSRQLSPFKHPKHWLWCTGIPRNPQGKVNRQQLRQWAAQTLGIPTQAS

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=318, Percent_Identity=25.4716981132075, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI38505220, Length=266, Percent_Identity=25.9398496240602, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI42544132, Length=246, Percent_Identity=26.0162601626016, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI122937307, Length=240, Percent_Identity=27.0833333333333, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI115511026, Length=237, Percent_Identity=27.4261603375527, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI58082049, Length=218, Percent_Identity=27.0642201834862, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI157311624, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157311622, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI187761345, Length=335, Percent_Identity=28.0597014925373, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI187761343, Length=335, Percent_Identity=28.0597014925373, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788595, Length=346, Percent_Identity=30.3468208092486, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693145, Length=355, Percent_Identity=23.943661971831, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI221142682, Length=208, Percent_Identity=28.8461538461538, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788107, Length=352, Percent_Identity=25.8522727272727, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786810, Length=181, Percent_Identity=29.2817679558011, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790505, Length=272, Percent_Identity=23.8970588235294, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=343, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=377, Percent_Identity=26.525198938992, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=302, Percent_Identity=27.8145695364238, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=326, Percent_Identity=26.0736196319018, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=363, Percent_Identity=25.3443526170799, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=319, Percent_Identity=25.0783699059561, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=319, Percent_Identity=23.8244514106583, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=319, Percent_Identity=23.8244514106583, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=319, Percent_Identity=23.8244514106583, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=329, Percent_Identity=24.9240121580547, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=313, Percent_Identity=26.1980830670927, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=310, Percent_Identity=27.0967741935484, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24653035, Length=228, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI161076582, Length=234, Percent_Identity=27.3504273504274, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581924, Length=314, Percent_Identity=24.203821656051, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356441, Length=310, Percent_Identity=23.8709677419355, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281365686, Length=333, Percent_Identity=24.6246246246246, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648255, Length=299, Percent_Identity=22.742474916388, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20130357, Length=268, Percent_Identity=29.1044776119403, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648260, Length=266, Percent_Identity=27.0676691729323, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI62471679, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI62471683, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI62471685, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24586636, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI62471689, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI62471681, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI62471687, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24586634, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI22026970, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922652, Length=363, Percent_Identity=23.1404958677686, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358303, Length=179, Percent_Identity=27.9329608938547, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51727; Mature: 51727

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIVLSGDGGDLKECLSKLEEQLRPLADKAVPLLVADSHPVQVWAAVLTCQRLGIPLFL
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEE
GNPHWGEREWGQALADMGSGWCWRQGRLEGDPAPLNPRLRELREVLPSSHWPLIGIPTGG
CCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCC
SGGQIKWAMHSWLTLSASAKAHQQHFAVGPVHSYCLLPLYHVSGLMQAVRSWLSGGQWVV
CCCEEEEEECCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WDWKRQQQAKAWDPPLPGSFLSLVPTQLQRLLLSAHPGVLESLRQFRAILVGGGPTWPSL
EECCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCHHH
RQQARQLHLPLALTYGMTETASQVCTLLPEAFWQGSDSLGRPLPHATLQIRSEKGDPLPS
HHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCC
HAVGYIHIQAESLALGYYPHLSATHRLREGQQQGFTPGDLGYLDRQGSLYWLGRADDLIL
CCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEECCCCEEE
TGGEKVMAAEVEGEIRASGWVEDVCVLGLPDAEWGQRVVAVVVPKTGIPKELEMHLKQHL
ECCCEEEEEECCCCCCCCCCHHCEEEECCCCCHHCCEEEEEEECCCCCCHHHHHHHHHHH
SRQLSPFKHPKHWLWCTGIPRNPQGKVNRQQLRQWAAQTLGIPTQAS
HHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRSIVLSGDGGDLKECLSKLEEQLRPLADKAVPLLVADSHPVQVWAAVLTCQRLGIPLFL
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEE
GNPHWGEREWGQALADMGSGWCWRQGRLEGDPAPLNPRLRELREVLPSSHWPLIGIPTGG
CCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCC
SGGQIKWAMHSWLTLSASAKAHQQHFAVGPVHSYCLLPLYHVSGLMQAVRSWLSGGQWVV
CCCEEEEEECCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WDWKRQQQAKAWDPPLPGSFLSLVPTQLQRLLLSAHPGVLESLRQFRAILVGGGPTWPSL
EECCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCHHH
RQQARQLHLPLALTYGMTETASQVCTLLPEAFWQGSDSLGRPLPHATLQIRSEKGDPLPS
HHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCC
HAVGYIHIQAESLALGYYPHLSATHRLREGQQQGFTPGDLGYLDRQGSLYWLGRADDLIL
CCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEECCCCEEE
TGGEKVMAAEVEGEIRASGWVEDVCVLGLPDAEWGQRVVAVVVPKTGIPKELEMHLKQHL
ECCCEEEEEECCCCCCCCCCHHCEEEECCCCCHHCCEEEEEEECCCCCCHHHHHHHHHHH
SRQLSPFKHPKHWLWCTGIPRNPQGKVNRQQLRQWAAQTLGIPTQAS
HHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]