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The map label for this gene is ycjG [H]

Identifier: 86609592

GI number: 86609592

Start: 2245179

End: 2246231

Strand: Direct

Name: ycjG [H]

Synonym: CYB_2145

Alternate gene names: 86609592

Gene position: 2245179-2246231 (Clockwise)

Preceding gene: 86609586

Following gene: 86609593

Centisome position: 73.69

GC content: 61.92

Gene sequence:

>1053_bases
ATGCGGCTCTACGTCGAACCTTTCACCGTTCACAAGCGCTACGCCTTGACCATCAGCCGAGGTACCCACAGCGATACAAC
CTTGGCTTGGGTGCGAATTGAGGAGGAGGGAGTTGAAGGGTGGGGAGAGGCTTGCCCTTTTTCGGTGAGCGACGACTACC
ACCAGTCTCTGGACGACATCCTGATGGGTCTGGAGGTGGCCAAAGGGATCCTGCGCCGCCATTCCCCCTGGGAGCGACAG
CCAATCGAGGCAGAACTGCTGCGGGCCGGGATCCCTTCGGCAGCGCGGGCAGCGGTGGATATGGCCCTGCACGATTGGAT
GGGCAAACGGCTGGGGAGGCCCCTGTGGCAGTTGTGGGGGCTGGATCGCTCCCGCATTCCGCCCACGACGATGACGATCG
GCATCTCCACCCCGCAGGCGGCCCGCCAGCGCGCCTTGGCCTGGATGGAACAGACCCAAGCGCGGGCCTTTAAGATCAAG
CTGGGCAGCGGCTCAGGACTGATGGCGGATCAGAACATGCTGATTGCTGTACAAGAGGTGATCCCGCTGGGATCCCTGAT
TACAGTGGATGCCAACGGCGCTTGGACGGTGGAGCAAACCCTGAGCATGAGCAACTGGCTGGCGGGGCAAGGGGTGAGCT
ACCTGGAGCAGCCTTTGGAGAGGGGGCAGGAAGCGAAGCTGATCACCCTCTGGCACGAGTCGAAGCTGCCCATTTTTGTG
GATGAGAGCTGTATGGACAGCCGCGATATCCCCCCTCTGGTGGATCGCGTCCACGGGATCAACATTAAGCTGATGAAGTG
CGGAGGCCTCAGCGAAGCCTGGCGCATGATCCACACCGCCAAAGCCCACGGCCTACAGGTGATGTTGGGCTGCTACAGCA
ACACAGCCCTGGGGAATACAGCGGCAGCCCACTTGGCTCCCCTGGTGGATTACCTGGACTTGGATAGCCACCTCAACCTC
ATCGACGATCCCTTTGCCGGGGCCATCCTGCAGGACGGCTGCCTGCTCCCCACCGACCGACCGGGTTTGGGGGTAGAGCT
GCGGGAGGAATAA

Upstream 100 bases:

>100_bases
GGCTTGGGGACGGGGGCAGGCTGGGCTGGGAACCAAGCTAAGCTCAAAGGGGCGGCCCAGGCCCAATTGCCCCTCCAAGC
TTCCTCTTCTGGAGATGCCC

Downstream 100 bases:

>100_bases
TGGGAAGGCCGGAATCCCTGTCCAGCGAAAGTCGCGTGGCCATCCTGCTCCACGGGGGATTGCTGGGGGAGCATGGGAAA
ACGGGGCTGGGGCTGCTGCG

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRLYVEPFTVHKRYALTISRGTHSDTTLAWVRIEEEGVEGWGEACPFSVSDDYHQSLDDILMGLEVAKGILRRHSPWERQ
PIEAELLRAGIPSAARAAVDMALHDWMGKRLGRPLWQLWGLDRSRIPPTTMTIGISTPQAARQRALAWMEQTQARAFKIK
LGSGSGLMADQNMLIAVQEVIPLGSLITVDANGAWTVEQTLSMSNWLAGQGVSYLEQPLERGQEAKLITLWHESKLPIFV
DESCMDSRDIPPLVDRVHGINIKLMKCGGLSEAWRMIHTAKAHGLQVMLGCYSNTALGNTAAAHLAPLVDYLDLDSHLNL
IDDPFAGAILQDGCLLPTDRPGLGVELREE

Sequences:

>Translated_350_residues
MRLYVEPFTVHKRYALTISRGTHSDTTLAWVRIEEEGVEGWGEACPFSVSDDYHQSLDDILMGLEVAKGILRRHSPWERQ
PIEAELLRAGIPSAARAAVDMALHDWMGKRLGRPLWQLWGLDRSRIPPTTMTIGISTPQAARQRALAWMEQTQARAFKIK
LGSGSGLMADQNMLIAVQEVIPLGSLITVDANGAWTVEQTLSMSNWLAGQGVSYLEQPLERGQEAKLITLWHESKLPIFV
DESCMDSRDIPPLVDRVHGINIKLMKCGGLSEAWRMIHTAKAHGLQVMLGCYSNTALGNTAAAHLAPLVDYLDLDSHLNL
IDDPFAGAILQDGCLLPTDRPGLGVELREE
>Mature_350_residues
MRLYVEPFTVHKRYALTISRGTHSDTTLAWVRIEEEGVEGWGEACPFSVSDDYHQSLDDILMGLEVAKGILRRHSPWERQ
PIEAELLRAGIPSAARAAVDMALHDWMGKRLGRPLWQLWGLDRSRIPPTTMTIGISTPQAARQRALAWMEQTQARAFKIK
LGSGSGLMADQNMLIAVQEVIPLGSLITVDANGAWTVEQTLSMSNWLAGQGVSYLEQPLERGQEAKLITLWHESKLPIFV
DESCMDSRDIPPLVDRVHGINIKLMKCGGLSEAWRMIHTAKAHGLQVMLGCYSNTALGNTAAAHLAPLVDYLDLDSHLNL
IDDPFAGAILQDGCLLPTDRPGLGVELREE

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=318, Percent_Identity=34.2767295597484, Blast_Score=127, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 38744; Mature: 38744

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYVEPFTVHKRYALTISRGTHSDTTLAWVRIEEEGVEGWGEACPFSVSDDYHQSLDDI
CEEEECCEEECEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
LMGLEVAKGILRRHSPWERQPIEAELLRAGIPSAARAAVDMALHDWMGKRLGRPLWQLWG
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHC
LDRSRIPPTTMTIGISTPQAARQRALAWMEQTQARAFKIKLGSGSGLMADQNMLIAVQEV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCEECCCCEEEEHHH
IPLGSLITVDANGAWTVEQTLSMSNWLAGQGVSYLEQPLERGQEAKLITLWHESKLPIFV
CCCCCEEEEECCCCEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEE
DESCMDSRDIPPLVDRVHGINIKLMKCGGLSEAWRMIHTAKAHGLQVMLGCYSNTALGNT
CCCCCCCCCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCH
AAAHLAPLVDYLDLDSHLNLIDDPFAGAILQDGCLLPTDRPGLGVELREE
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCEECCCCCCCCCCEECCC
>Mature Secondary Structure
MRLYVEPFTVHKRYALTISRGTHSDTTLAWVRIEEEGVEGWGEACPFSVSDDYHQSLDDI
CEEEECCEEECEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
LMGLEVAKGILRRHSPWERQPIEAELLRAGIPSAARAAVDMALHDWMGKRLGRPLWQLWG
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHC
LDRSRIPPTTMTIGISTPQAARQRALAWMEQTQARAFKIKLGSGSGLMADQNMLIAVQEV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCEECCCCEEEEHHH
IPLGSLITVDANGAWTVEQTLSMSNWLAGQGVSYLEQPLERGQEAKLITLWHESKLPIFV
CCCCCEEEEECCCCEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEE
DESCMDSRDIPPLVDRVHGINIKLMKCGGLSEAWRMIHTAKAHGLQVMLGCYSNTALGNT
CCCCCCCCCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCH
AAAHLAPLVDYLDLDSHLNLIDDPFAGAILQDGCLLPTDRPGLGVELREE
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCEECCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]