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The map label for this gene is selU [H]

Identifier: 86609458

GI number: 86609458

Start: 2113998

End: 2115062

Strand: Reverse

Name: selU [H]

Synonym: CYB_2008

Alternate gene names: 86609458

Gene position: 2115062-2113998 (Counterclockwise)

Preceding gene: 86609459

Following gene: 86609456

Centisome position: 69.42

GC content: 59.53

Gene sequence:

>1065_bases
GTGTCTCCTCCGATTCACTTCACTGCAGAGCCCTGGCTGCACACCTACAGCGAAATTATCGATGTGCGCTCTCCGGCAGA
GTTCGCAGAGGATCATTGGCCGGGAGCCATCAACTTGCCTGTACTCTACGATACAGAACGGGCGGAGGTGGGCACGCTCT
ACAAGCAGGTGGATCCCTTTACAGCCCGCAAACGAGGGGCAAGCTTGGTGGCCCAGAACATCAGTCGCCACTTGCAAACC
CATTTTGCCGATAAAGATCGCGATTACCGGCCCCTAATCTACTGCTGGCGGGGAGGGCAGCGCTCTGCCAGCTTGGCATT
GGTGTTGTCCCAGGTGGGCTGGCAGGTTACGGTTTTGCAAGGTGGCTACAAAACCTACCGGGCCTGGGTGCGCCGACAGC
TGCAGATCTTGCCCCAGGAATTTACTTACCGAGTGCTCTGCGGACCAACAGGGAGCGGCAAAACCCAGTTGCTTCACCAT
CTGCGCCAACAGGGATCCCAAGTCCTCGATCTGGAAAAGCTGGCCTGTCACCGGGGATCCCTCCTGGGGGCCGAGTGGGG
GGTGCCACAGCCCGGTCAGAAGAGCTTTGAAAGTCAACTGGTGCAGGTTTTTCTCCAGTGGGATCCCGCCAAGCCAGTCT
GGGTGGAGGCTGAGAGTGCCAAAGTGGGCCAGCTCCATCTGCCGCCTGCCCTTTGGCAAGCCCTTACCCAAGCCGACTGT
GTCGAAGTGCAGTTGCCTCTAGAGGAGCGGGTGAAGGGGCTGATCGCCCACTATCCCCACTGGATTGCTCACCCCCAAGC
GCTAAAGGGGAAATTGCTGCCTCTGAAGTCCCGTCTTGGCCAGGCCCGCCTGCAGCGTTGGTTTGAGCTGATCGACGCCC
AAGACTGGCCGCAGTTTGTGCAGGAGTTGCTGGAGCAGCACTATGACCCCACCTATCGGCACGGGCTGCAGCAAAACTTC
AATCGCCTTCGGGCCCGGATTGAGCTGTCTGACCTTTCCGAGACCAGCCTCAGGGAAGCTGCTGCTCGCCTCATCCAGAT
GCCGCTTGGATCCCTGGCTAGGTGA

Upstream 100 bases:

>100_bases
GATAGGCTAGGGTTAGCCCTGGGCTTCCTTGAGCAGCAGCGTTGGCTAAGCTAGAAACTGTTCAAGATTGCGCTAGCTTG
CCATTTCCATCCACAGGTTT

Downstream 100 bases:

>100_bases
GCACAATAAACTCCAAAGCCCGCAACACCTCAAACCACTCGGAAACTTCCAGCCGAACCGTGGAATCGGCAATCCACCGG
CAGCCGGGATAAAAAGACTT

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSPPIHFTAEPWLHTYSEIIDVRSPAEFAEDHWPGAINLPVLYDTERAEVGTLYKQVDPFTARKRGASLVAQNISRHLQT
HFADKDRDYRPLIYCWRGGQRSASLALVLSQVGWQVTVLQGGYKTYRAWVRRQLQILPQEFTYRVLCGPTGSGKTQLLHH
LRQQGSQVLDLEKLACHRGSLLGAEWGVPQPGQKSFESQLVQVFLQWDPAKPVWVEAESAKVGQLHLPPALWQALTQADC
VEVQLPLEERVKGLIAHYPHWIAHPQALKGKLLPLKSRLGQARLQRWFELIDAQDWPQFVQELLEQHYDPTYRHGLQQNF
NRLRARIELSDLSETSLREAAARLIQMPLGSLAR

Sequences:

>Translated_354_residues
MSPPIHFTAEPWLHTYSEIIDVRSPAEFAEDHWPGAINLPVLYDTERAEVGTLYKQVDPFTARKRGASLVAQNISRHLQT
HFADKDRDYRPLIYCWRGGQRSASLALVLSQVGWQVTVLQGGYKTYRAWVRRQLQILPQEFTYRVLCGPTGSGKTQLLHH
LRQQGSQVLDLEKLACHRGSLLGAEWGVPQPGQKSFESQLVQVFLQWDPAKPVWVEAESAKVGQLHLPPALWQALTQADC
VEVQLPLEERVKGLIAHYPHWIAHPQALKGKLLPLKSRLGQARLQRWFELIDAQDWPQFVQELLEQHYDPTYRHGLQQNF
NRLRARIELSDLSETSLREAAARLIQMPLGSLAR
>Mature_353_residues
SPPIHFTAEPWLHTYSEIIDVRSPAEFAEDHWPGAINLPVLYDTERAEVGTLYKQVDPFTARKRGASLVAQNISRHLQTH
FADKDRDYRPLIYCWRGGQRSASLALVLSQVGWQVTVLQGGYKTYRAWVRRQLQILPQEFTYRVLCGPTGSGKTQLLHHL
RQQGSQVLDLEKLACHRGSLLGAEWGVPQPGQKSFESQLVQVFLQWDPAKPVWVEAESAKVGQLHLPPALWQALTQADCV
EVQLPLEERVKGLIAHYPHWIAHPQALKGKLLPLKSRLGQARLQRWFELIDAQDWPQFVQELLEQHYDPTYRHGLQQNFN
RLRARIELSDLSETSLREAAARLIQMPLGSLAR

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=349, Percent_Identity=34.6704871060172, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 40511; Mature: 40380

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPIHFTAEPWLHTYSEIIDVRSPAEFAEDHWPGAINLPVLYDTERAEVGTLYKQVDPF
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCEECCCEEECCCHHHHHHHHHHCCCH
TARKRGASLVAQNISRHLQTHFADKDRDYRPLIYCWRGGQRSASLALVLSQVGWQVTVLQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEE
GGYKTYRAWVRRQLQILPQEFTYRVLCGPTGSGKTQLLHHLRQQGSQVLDLEKLACHRGS
CCHHHHHHHHHHHHHHCCHHCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
LLGAEWGVPQPGQKSFESQLVQVFLQWDPAKPVWVEAESAKVGQLHLPPALWQALTQADC
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCHHHHHHHHHCCC
VEVQLPLEERVKGLIAHYPHWIAHPQALKGKLLPLKSRLGQARLQRWFELIDAQDWPQFV
EEEECCHHHHHHHHHHHCCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
QELLEQHYDPTYRHGLQQNFNRLRARIELSDLSETSLREAAARLIQMPLGSLAR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPPIHFTAEPWLHTYSEIIDVRSPAEFAEDHWPGAINLPVLYDTERAEVGTLYKQVDPF
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCEECCCEEECCCHHHHHHHHHHCCCH
TARKRGASLVAQNISRHLQTHFADKDRDYRPLIYCWRGGQRSASLALVLSQVGWQVTVLQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEE
GGYKTYRAWVRRQLQILPQEFTYRVLCGPTGSGKTQLLHHLRQQGSQVLDLEKLACHRGS
CCHHHHHHHHHHHHHHCCHHCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
LLGAEWGVPQPGQKSFESQLVQVFLQWDPAKPVWVEAESAKVGQLHLPPALWQALTQADC
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCHHHHHHHHHCCC
VEVQLPLEERVKGLIAHYPHWIAHPQALKGKLLPLKSRLGQARLQRWFELIDAQDWPQFV
EEEECCHHHHHHHHHHHCCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
QELLEQHYDPTYRHGLQQNFNRLRARIELSDLSETSLREAAARLIQMPLGSLAR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]