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The map label for this gene is pilG [H]

Identifier: 86609395

GI number: 86609395

Start: 2030529

End: 2031671

Strand: Reverse

Name: pilG [H]

Synonym: CYB_1939

Alternate gene names: 86609395

Gene position: 2031671-2030529 (Counterclockwise)

Preceding gene: 86609397

Following gene: 86609394

Centisome position: 66.68

GC content: 57.22

Gene sequence:

>1143_bases
ATGCAAGGAAAACTACAAGAGATCGATATCCGCAGCATCCTTCAGCTCATCGAGCTGGGCCAGCGCACCGGTGAGCTTTT
TGTAGAGGCCAGCCCCTCTGTCTACTGGTTTGTCTTCTTCCTGAATGGGCGGATTATCTACGCCTCTGAAACCGAAGGGA
ACATTGCCCGCCTCAGAGACTATCTGCACCGCTTCAAGCTGGAAGCCGCCCTGGAGGAAATTCAAATTCCCCACAAAGCC
GCGGTCAACGCCCCCGAATACAACCGCCTCTGGTCTTTGCTGGAAAGCCACAAGCTCAACCCCAACCAAGGCAAGCAAAT
CCTGCTGGGCATGGTGCGGGAAGTCCTCTTCGACCTGCTTAGCCTCCACCACGGCTCCTTCATTTTTGAGGCCGGATCCC
CCCTGGCCCCCCAGTTGACCACCTTCCCCGTTACCCCCCTGCTCACCGAGATCGTAGCCCAAGTGCAGGAGTGGAAAAAG
CTGCACCCCTACATCCAAGCTCCCAACCAAGCTCCCACCATTGTCAACCCCCTGCCGCTCCAGCAAAACCTGCCCGCCCC
CGTCTTCAAAGCCCTGAGCGAGTGGTCAGACGGCAAAACCAGCATTCGCCAGATGGGTCGCTACCTGGGTCGAGACACCC
TCACAGTGGCCAAGGCCATCTATCCCTACGTCCGCCAAGGGGCCATTCAACTGTCCGAGCCCGTCTTGATCGCGGAGCCC
AAGCCCAGCCGTCCCCTCAAACCCAGCGATAAGCCCAGCCACATCCTTTGCATCGACGACTCCATGGCCATCCAAAAAAC
CGTAGAGTTCATCCTGCAAAACGAGGGCTACCAGGTCACCACCATCGGCAATCCTCTCAAAGCCTTGGGACAGGTGTTTC
TCCTCAACCCAGATATGATCCTCTGTGATGTGGCCATGCCCAAATTGGATGGGTATGAGCTGTGCGCCATGTTGCGCAAA
TCCAGCCAGTTTCGCGAAACCCCCATGATCATGCTGACCGGCAAGGATGGCTTCACCGATCGGATTAAGGCCCGCATGGC
CGGAGCGAACGAATTTTTGAGCAAACCCTTTGCGGAAAAGGAGTTGTTGACCATTGTCGAATCCTACGTGGGCCCTCCCC
CCAAACGGGGCGGTGGCGAATAG

Upstream 100 bases:

>100_bases
TTTAACCCTCTTCCCGAAAATGGCCTTACCGGCGGGGCAGCAACAGTTAAAGACTAGCGTCATAATGGACGTATCTTGCT
GGACTGCAGGCAGGGATCCC

Downstream 100 bases:

>100_bases
GCGTTGGGGTAGCCAAAATGTTGGCACAGAAGACTGTGGGCGGGGTACTGGACAGAAGAGTGGCCTCACCGAAACAATAG
GGGTAGCATGGCTGACTTGG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MQGKLQEIDIRSILQLIELGQRTGELFVEASPSVYWFVFFLNGRIIYASETEGNIARLRDYLHRFKLEAALEEIQIPHKA
AVNAPEYNRLWSLLESHKLNPNQGKQILLGMVREVLFDLLSLHHGSFIFEAGSPLAPQLTTFPVTPLLTEIVAQVQEWKK
LHPYIQAPNQAPTIVNPLPLQQNLPAPVFKALSEWSDGKTSIRQMGRYLGRDTLTVAKAIYPYVRQGAIQLSEPVLIAEP
KPSRPLKPSDKPSHILCIDDSMAIQKTVEFILQNEGYQVTTIGNPLKALGQVFLLNPDMILCDVAMPKLDGYELCAMLRK
SSQFRETPMIMLTGKDGFTDRIKARMAGANEFLSKPFAEKELLTIVESYVGPPPKRGGGE

Sequences:

>Translated_380_residues
MQGKLQEIDIRSILQLIELGQRTGELFVEASPSVYWFVFFLNGRIIYASETEGNIARLRDYLHRFKLEAALEEIQIPHKA
AVNAPEYNRLWSLLESHKLNPNQGKQILLGMVREVLFDLLSLHHGSFIFEAGSPLAPQLTTFPVTPLLTEIVAQVQEWKK
LHPYIQAPNQAPTIVNPLPLQQNLPAPVFKALSEWSDGKTSIRQMGRYLGRDTLTVAKAIYPYVRQGAIQLSEPVLIAEP
KPSRPLKPSDKPSHILCIDDSMAIQKTVEFILQNEGYQVTTIGNPLKALGQVFLLNPDMILCDVAMPKLDGYELCAMLRK
SSQFRETPMIMLTGKDGFTDRIKARMAGANEFLSKPFAEKELLTIVESYVGPPPKRGGGE
>Mature_380_residues
MQGKLQEIDIRSILQLIELGQRTGELFVEASPSVYWFVFFLNGRIIYASETEGNIARLRDYLHRFKLEAALEEIQIPHKA
AVNAPEYNRLWSLLESHKLNPNQGKQILLGMVREVLFDLLSLHHGSFIFEAGSPLAPQLTTFPVTPLLTEIVAQVQEWKK
LHPYIQAPNQAPTIVNPLPLQQNLPAPVFKALSEWSDGKTSIRQMGRYLGRDTLTVAKAIYPYVRQGAIQLSEPVLIAEP
KPSRPLKPSDKPSHILCIDDSMAIQKTVEFILQNEGYQVTTIGNPLKALGQVFLLNPDMILCDVAMPKLDGYELCAMLRK
SSQFRETPMIMLTGKDGFTDRIKARMAGANEFLSKPFAEKELLTIVESYVGPPPKRGGGE

Specific function: Is involved in pilus biosynthesis and twitching motility. May act as a single-domain response regulator whose function may be to receive a certain environmental signal(s) and then transduce that signal to the pilus assembly machinery via specific protein-

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=30.7017543859649, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87082012, Length=114, Percent_Identity=32.4561403508772, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1786911, Length=110, Percent_Identity=31.8181818181818, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789809, Length=114, Percent_Identity=31.5789473684211, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790860, Length=127, Percent_Identity=28.3464566929134, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 42629; Mature: 42629

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGKLQEIDIRSILQLIELGQRTGELFVEASPSVYWFVFFLNGRIIYASETEGNIARLRD
CCCCCHHCCHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCEEEEEECCCCCHHHHHH
YLHRFKLEAALEEIQIPHKAAVNAPEYNRLWSLLESHKLNPNQGKQILLGMVREVLFDLL
HHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
SLHHGSFIFEAGSPLAPQLTTFPVTPLLTEIVAQVQEWKKLHPYIQAPNQAPTIVNPLPL
HHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCC
QQNLPAPVFKALSEWSDGKTSIRQMGRYLGRDTLTVAKAIYPYVRQGAIQLSEPVLIAEP
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCCEEEECC
KPSRPLKPSDKPSHILCIDDSMAIQKTVEFILQNEGYQVTTIGNPLKALGQVFLLNPDMI
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCHHHHHCEEEEECCCEE
LCDVAMPKLDGYELCAMLRKSSQFRETPMIMLTGKDGFTDRIKARMAGANEFLSKPFAEK
EEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCHHHHHHCCCCHH
ELLTIVESYVGPPPKRGGGE
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQGKLQEIDIRSILQLIELGQRTGELFVEASPSVYWFVFFLNGRIIYASETEGNIARLRD
CCCCCHHCCHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCEEEEEECCCCCHHHHHH
YLHRFKLEAALEEIQIPHKAAVNAPEYNRLWSLLESHKLNPNQGKQILLGMVREVLFDLL
HHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
SLHHGSFIFEAGSPLAPQLTTFPVTPLLTEIVAQVQEWKKLHPYIQAPNQAPTIVNPLPL
HHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCC
QQNLPAPVFKALSEWSDGKTSIRQMGRYLGRDTLTVAKAIYPYVRQGAIQLSEPVLIAEP
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCCEEEECC
KPSRPLKPSDKPSHILCIDDSMAIQKTVEFILQNEGYQVTTIGNPLKALGQVFLLNPDMI
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCHHHHHCEEEEECCCEE
LCDVAMPKLDGYELCAMLRKSSQFRETPMIMLTGKDGFTDRIKARMAGANEFLSKPFAEK
EEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCHHHHHHCCCCHH
ELLTIVESYVGPPPKRGGGE
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8104179; 10984043 [H]