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The map label for this gene is cefD [H]

Identifier: 86609330

GI number: 86609330

Start: 1957778

End: 1958914

Strand: Reverse

Name: cefD [H]

Synonym: CYB_1874

Alternate gene names: 86609330

Gene position: 1958914-1957778 (Counterclockwise)

Preceding gene: 86609331

Following gene: 86609328

Centisome position: 64.3

GC content: 60.6

Gene sequence:

>1137_bases
TTGTGGGGACTGGATCCGCAAGCCGTTTTTTTGAATCACGGCTCCTACGGCGCGACCCCGAAAAAGGTGTTGCAGGCCCA
GAGTGTATGGCGGGAACGCCTTGAGGCTCAGCCGGTGCAGTTTATGGGAGAGGAACTTCCTCGAGCCTTGCGGGCGGCGG
CAGCAGAACTGGCCCATTTCGTCGGGGCCGAACCCGAAAATTTGGTGTTTGTGGAAAATGCCACCAGCGGGGTCAATGCC
GTTTTGCGCTCGCTGCGGTTCCGGCCAGAGGATCAGATCGCCTGCACCAATCACGGTTACGGGGCCGTTCGTCAGGCATT
GCAGTACATCTGTGCCCAGTGGGGAGCCATCCCAGTTGAGGCGCAGATCCCTTTCCCCATTGCGGGGCCAGAGCAGGTTA
TCGCGGCCTTCGAGGCCATCCTCACTCCCCAAACTCGCCTGGCGGTGTTGGATCACCTCACCTCGCCTACGGCTCTGATC
TACCCCCTGCCTGAGCTGATCGGCCTCTGTCGGGAGCGCGGGATCCCGGTTTTGGTGGATGGCGCCCATGCCCCCGGAGT
TCTCCCCTTGGAGTTGGAAAAGCTAGGAGCCGATTGGTACACCGGCAATGCCCACAAGTGGCTGTTTGCCCCGAAAGGCT
GTGCCTTTTTGTGGGTGGCCCCCTATCGACAGGCCCAAACCCATCCTACGGTGATCTCCCACGGCTATAGGCAAGGGTTC
ACAGCGGAGTTCGACTGGGTGGGCACACGGGATCCCAGCGCTTGGTTGGCCATTTCAGCAGCCCTGGCGTTTATCCAAGG
GGTGGGACTGGACAATATCCGTCAGCACAACCACACTCTGCTGCTGCAGGCGCGGCAGTTGCTCTTGGAGGCTCTTGAGG
GGATCCCGCCCGCCCCCGAAAAGATGTTGGGCTTTATGGCCACCCTACCCTTGCCGCCATTTTGGCAGCAGTGGATCCCT
GAGCTTCCTCTTGAGGAGCGCGCCCGTCGGCTTCACGATTACCTCTGGTACAAGTACCGCATTGAGGTTCCGATCATCCC
CTTCGCCGGGCAGCTTTGGGTACGCATCTCGGCCCAGGTCTACAACCGTTTGGCAGAGTACGAACAGTTGGCGCTGGCCT
TGCAGCATCTGCCCTGA

Upstream 100 bases:

>100_bases
ACAGAGGGAGTGAACCATGCCTTTCTTCTCCAACCTGGGATCCGATTCTCCCCACTTTGGCGCTGCCCTCAGTGCTTGTG
CGCCACTTTGCGAACGCTCC

Downstream 100 bases:

>100_bases
GGTTTTGTCGGTTGAGCCGCTTTCACGACATTGCAAGTATAGTTGCTTCGCACACCAGTAAAACGATTCCAGATCTATGG
TCAATTCAGTTGAGGATGAG

Product: isopenicillin N-epimerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MWGLDPQAVFLNHGSYGATPKKVLQAQSVWRERLEAQPVQFMGEELPRALRAAAAELAHFVGAEPENLVFVENATSGVNA
VLRSLRFRPEDQIACTNHGYGAVRQALQYICAQWGAIPVEAQIPFPIAGPEQVIAAFEAILTPQTRLAVLDHLTSPTALI
YPLPELIGLCRERGIPVLVDGAHAPGVLPLELEKLGADWYTGNAHKWLFAPKGCAFLWVAPYRQAQTHPTVISHGYRQGF
TAEFDWVGTRDPSAWLAISAALAFIQGVGLDNIRQHNHTLLLQARQLLLEALEGIPPAPEKMLGFMATLPLPPFWQQWIP
ELPLEERARRLHDYLWYKYRIEVPIIPFAGQLWVRISAQVYNRLAEYEQLALALQHLP

Sequences:

>Translated_378_residues
MWGLDPQAVFLNHGSYGATPKKVLQAQSVWRERLEAQPVQFMGEELPRALRAAAAELAHFVGAEPENLVFVENATSGVNA
VLRSLRFRPEDQIACTNHGYGAVRQALQYICAQWGAIPVEAQIPFPIAGPEQVIAAFEAILTPQTRLAVLDHLTSPTALI
YPLPELIGLCRERGIPVLVDGAHAPGVLPLELEKLGADWYTGNAHKWLFAPKGCAFLWVAPYRQAQTHPTVISHGYRQGF
TAEFDWVGTRDPSAWLAISAALAFIQGVGLDNIRQHNHTLLLQARQLLLEALEGIPPAPEKMLGFMATLPLPPFWQQWIP
ELPLEERARRLHDYLWYKYRIEVPIIPFAGQLWVRISAQVYNRLAEYEQLALALQHLP
>Mature_378_residues
MWGLDPQAVFLNHGSYGATPKKVLQAQSVWRERLEAQPVQFMGEELPRALRAAAAELAHFVGAEPENLVFVENATSGVNA
VLRSLRFRPEDQIACTNHGYGAVRQALQYICAQWGAIPVEAQIPFPIAGPEQVIAAFEAILTPQTRLAVLDHLTSPTALI
YPLPELIGLCRERGIPVLVDGAHAPGVLPLELEKLGADWYTGNAHKWLFAPKGCAFLWVAPYRQAQTHPTVISHGYRQGF
TAEFDWVGTRDPSAWLAISAALAFIQGVGLDNIRQHNHTLLLQARQLLLEALEGIPPAPEKMLGFMATLPLPPFWQQWIP
ELPLEERARRLHDYLWYKYRIEVPIIPFAGQLWVRISAQVYNRLAEYEQLALALQHLP

Specific function: Catalyzes the reversible isomerization between isopenicillin N and penicillin N [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1787970, Length=325, Percent_Identity=28, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =5.1.1.17 [H]

Molecular weight: Translated: 42262; Mature: 42262

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWGLDPQAVFLNHGSYGATPKKVLQAQSVWRERLEAQPVQFMGEELPRALRAAAAELAHF
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VGAEPENLVFVENATSGVNAVLRSLRFRPEDQIACTNHGYGAVRQALQYICAQWGAIPVE
HCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEEE
AQIPFPIAGPEQVIAAFEAILTPQTRLAVLDHLTSPTALIYPLPELIGLCRERGIPVLVD
EECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCEEEE
GAHAPGVLPLELEKLGADWYTGNAHKWLFAPKGCAFLWVAPYRQAQTHPTVISHGYRQGF
CCCCCCCCEEHHHHCCCCCEECCCCEEEECCCCCEEEEECCCCCCCCCCHHHHCCHHCCC
TAEFDWVGTRDPSAWLAISAALAFIQGVGLDNIRQHNHTLLLQARQLLLEALEGIPPAPE
CEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
KMLGFMATLPLPPFWQQWIPELPLEERARRLHDYLWYKYRIEVPIIPFAGQLWVRISAQV
HHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHEEEEEEEEEEECCCCHHHEEEHHHH
YNRLAEYEQLALALQHLP
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWGLDPQAVFLNHGSYGATPKKVLQAQSVWRERLEAQPVQFMGEELPRALRAAAAELAHF
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VGAEPENLVFVENATSGVNAVLRSLRFRPEDQIACTNHGYGAVRQALQYICAQWGAIPVE
HCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEEE
AQIPFPIAGPEQVIAAFEAILTPQTRLAVLDHLTSPTALIYPLPELIGLCRERGIPVLVD
EECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCEEEE
GAHAPGVLPLELEKLGADWYTGNAHKWLFAPKGCAFLWVAPYRQAQTHPTVISHGYRQGF
CCCCCCCCEEHHHHCCCCCEECCCCEEEECCCCCEEEEECCCCCCCCCCHHHHCCHHCCC
TAEFDWVGTRDPSAWLAISAALAFIQGVGLDNIRQHNHTLLLQARQLLLEALEGIPPAPE
CEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
KMLGFMATLPLPPFWQQWIPELPLEERARRLHDYLWYKYRIEVPIIPFAGQLWVRISAQV
HHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHEEEEEEEEEEECCCCHHHEEEHHHH
YNRLAEYEQLALALQHLP
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8437592 [H]